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Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A

Journal of Integrative Plant Biology 2024年第9期66卷 1915-1933页

作者： Miqi Xu Yi‐Yuan Wang Yujie wu Xiuhong Zhou Ziyan shan Kunying Tao Kaiqiang Qian Xuncheng Wang Jian Li Qingqing wu Xing Wang Deng Jun‐Jie Ling National Engineering Laboratory of Crop Stress Resistance Breeding School of Life SciencesAnhui Agricultural UniversityHefei 230036China Province Key Laboratory of Resource Insect Biology and Innovative Utilization School of Life SciencesAnhui Agricultural UniversityHefei 230036China Biotechnology Center State Key Laboratory of Tea Plant Biology and UtilizationSchool of Tea and Food Sciences and TechnologyAnhui Agricultural UniversityHefei 230036China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Life Sciences Nanjing Normal UniversityNanjing 210023China State Key Laboratory of Protein and Plant Gene Research Peking–Tsinghua Center for Life SciencesSchool of Advanced Agriculture Sciencesand School of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Wheat Improvement Shandong Laboratory of Advanced Agricultural Sciences at WeifangPeking University Institute of Advanced Agricultural SciencesWeifang 261000China

Although green light(GL)is located in the middle of the visible light spectrum and regulates a series of plant developmental processes,the mechanism by which it regulates seedling development is largely *** this study,we demonstrated that GL promotes atypical photomorphogenesis in Arabidopsis thaliana via the dual regulations of phytochrome B(phyB)and *** the Pr-to-Pfr conversion rates of phyB and phyA under GL were lower than those under red light(RL)in a fluence rate-dependent and time-dependent manner,long-term treatment with GL induced high Pfr/Pr ratios of phyB and ***,GL induced the formation of numerous small phyB photobodies in the nucleus,resulting in atypical photomorphogenesis,with smaller cotyledon opening angles and longer hypocotyls in seedlings compared to *** abundance of phyA significantly decreased after short-and long-term GL *** determined that four major PHYTOCHROME-INTERACTING FACTORs(PIFs:PIF1,PIF3,PIF4,and PIF5)act downstream of phyB in GL-mediated cotyledon *** addition,GL plays opposite roles in regulating different *** example,under continuous GL,the protein levels of all PIFs decreased,whereas the transcript levels of PIF4 and PIF5 strongly increased compared with dark *** together,our work provides a detailed molecular framework for understanding the role of the antagonistic regulations of phyB and phyA in GL-mediated atypical photomorphogenesis.

关键词： cotyledon development green light photobody phytochrome PIF3

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Differentiation and immunosuppressive function of CD19^(+)CD24^(hi)CD27^(+) regulatory B cells are regulated through the miR-29a-3p/NFAT5 pathway

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Hepatobiliary & Pancreatic Diseases International 2024年第5期23卷 472-480页

作者： Jin-Yang Li Tian-Shuo Feng Ji Gao Xin-Xiang Yang Xiang-Cheng Li Zhen-Hua Deng Yong-Xiang Xia Zheng-shan wu Hepatobiliary Center the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China Key Laboratory of Liver Transplantation Chinese Academy of Medical SciencesNanjing 210029China NHC Key Laboratory of Living Donor Liver Transplantation Nanjing Medical UniversityNanjing 210029China

Background: Regulatory B cells(Bregs) is an indispensable element in inducing immune tolerance after liver transplantation. As one of the microRNAs(miRNAs), mi R-29a-3p also inhibits translation by degrading the target mRNA, and yet the relationship between Bregs and mi R-29a-3p has not yet been fully explored. This study aimed to investigate the impact of miR-29a-3p on the regulation of differentiation and immunosuppressive functions of memory Bregs(m Bregs) and ultimately provide potentially effective therapies in inducing immune tolerance after liver transplantation. Methods: Flow cytometry was employed to determine the levels of Bregs in peripheral blood mononuclear cells. TaqMan low-density array miRNA assays were used to identify the expression of different miRNAs, electroporation transfection was used to induce mi R-29a-3p overexpression and knockdown, and dual luciferase reporter assay was used to verify the target gene of miR-29a-3p. Results: In patients experiencing acute rejection after liver transplantation, the proportions and immunosuppressive function of m Bregs in the circulating blood were significantly impaired. mi R-29a-3p was found to be a regulator of m Bregs differentiation. Inhibition of miR-29a-3p, which targeted nuclear factor of activated T cells 5(NFAT5), resulted in a conspicuous boost in the differentiation and immunosuppressive function of m Bregs. The inhibition of mi R-29a-3p in CD19~+ B cells was capable of raising the expression levels of NFAT5, thereby promoting B cells to differentiate into m Bregs. In addition, the observed enhancement of differentiation and immunosuppressive function of m Bregs upon mi R-29a-3p inhibition was abolished by the knockdown of NFAT5 in B cells. Conclusions: mi R-29a-3p was found to be a crucial regulator for m Bregs differentiation and immunosuppressive function. Silencing mi R-29a-3p could be a potentially effective therapeutic strategy for inducing immune tolerance after liver transplantation.

关键词： Regulatory B cells miR-29a-3p NFAT5 Liver transplantation

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中国城镇劳动力工资收入差距的长期演变

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财经问题研究 2023年第7期 16-30页

作者：李实吴珊珊邢春冰浙江大学公共管理学院浙江杭州310030 浙江大学长三角智慧绿洲创新中心浙江嘉兴314102 首都经济贸易大学劳动经济学院北京100070 中国人民大学农业与农村发展学院北京100086

随着中国经济的转型和共同富裕的不断推进,城镇劳动力工资收入差距不断扩大受到了学术界和管理层的广泛关注。本文使用1988—2018年中国家庭收入调查(CHIP)数据,考察了中国城镇劳动力工资收入差距的长期演变及其影响因素。研究结果表明,... 详细信息

随着中国经济的转型和共同富裕的不断推进,城镇劳动力工资收入差距不断扩大受到了学术界和管理层的广泛关注。本文使用1988—2018年中国家庭收入调查(CHIP)数据,考察了中国城镇劳动力工资收入差距的长期演变及其影响因素。研究结果表明,1988—2018年,中国城镇劳动力工资收入差距不断扩大。本文采用三种方法考察中国城镇劳动力工资收入差距扩大的影响因素,其中,采用明瑟(Mincer)工资方程的回归结果表明,城镇劳动力受教育程度对工资收入的影响持续上升;运用Shorrocks分解方法发现,受教育程度在工资收入分配中起到的作用不断加强,地区差异带来的影响不断弱化;使用反事实方法在将工资收入差距分解为条件分布(价格效应)和特征分布(组成效应)后发现,价格效应在工资收入差距扩大中起主导作用。本文的研究结果对于理解中国劳动力市场的演变、工资收入分配变化以及推动共同富裕具有重要参考价值。

关键词：工资收入差距劳动力受教育程度 Shorrocks分解反事实分解方法

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Integrated Chinese and Western Medicine in Treatment of Bronchopleural Fistula with Mycobacterium abscessus:A Case Report

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Chinese Journal of Integrative Medicine 2024年第8期30卷 742-746页

作者： WANG Qian-yun CHENG Miao BAN Cheng-jun ZHANG Li-shan WANG Hong-wu YANG Bing ZOU Heng WANG Ming-zhe The First Clinical Medical College of Beijing University of Chinese Medicine Beijing100700China Department of Respiratory Dongzhimen HospitalBeijing University of Chinese MedicineBeijing100700China

Bronchopleural fistula(BPF)is defined as a pathological connection between the trachea or bronchus and the pleural *** may occur as a serious complication after lung surgery.^((1))It has a low incidence of 1%-3%^((2.3... 详细信息

关键词： pleural lung surgery

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Research on Early Warning of Banking Crises from the Perspective of Credit Structures

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Contemporary Social Sciences 2024年第3期9卷 45-63页

作者： Zhou Yuqin Luo Zixuan wu shan Chongqing Normal University Nanjing University of Finance and Economics

The relationship between credit expansion and banking crises is complex and cannot be fully explained by total credit alone.A systematic analysis of the relationship between the amount and structure of total credit and banking crises is important for an objective prediction of the influence of potential financial *** paper,drawing on data from 15 selected countries,delves into the power of credit indicators in the early warning of banking crises from the perspectives of industrial structure,sector structure,and term structure of *** machine learning methods were used,including Logistic Regression,Random Forest,Decision Tree,Support Vector Machine(SVM),Bagging,and Boosting *** empirical findings indicate that credit expansion plays a crucial role in triggering banking ***,total credit is better suited for the early warning of short-term banking crises,whereas credit structure is more useful for the early warning of long-term banking ***,in an early warning system,identifying key early warning indicators is more meaningful than merely increasing the number of *** learning can somewhat enhance the early warning power,but it may not always be ***,more attention should be paid to potential systemic banking crises resulting from an imbalance in credit structure while regulating the total credit threshold.

关键词： banking crises credit expansion transnational empirical evidence structural perspective

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引入三相不平衡度的低压电网理论线损计算

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电力自动化设备 2007年第11期27卷 51-54页

作者：郭峰姚莉娜刘恒吴珊珊福建省电力勘测设计院线路设计部福建福州350003 郑州轻工业学院电气信息工程学院河南郑州450002 西安理工大学电气工程系陕西西安710048

提出采用均方根电流法计算低压电网理论线损,通过分析三相不平衡线路的线损,推导出线损与不平衡度的关系公式,并将该公式用于三相不平衡配电线路的理论线损计算。系统引入三相不平衡度采集装置,以单片机为主控制芯片,给出了系统结构框... 详细信息

提出采用均方根电流法计算低压电网理论线损,通过分析三相不平衡线路的线损,推导出线损与不平衡度的关系公式,并将该公式用于三相不平衡配电线路的理论线损计算。系统引入三相不平衡度采集装置,以单片机为主控制芯片,给出了系统结构框图。在采集三相电流时,使用该采集装置挂接在变压器出口端,定时实时采集和存储三相电流,以供计算三相不平衡度。以某台区实际运行为研究实例,介绍了计算步骤,计算结果表明所提出的计算系统是可行的。

关键词：三相不平衡线路线损率不平衡度

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痰热清注射液治疗全身炎症反应综合征的系统评价

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山东医药 2015年第39期55卷 79-81页

作者：武珊珊于强天津医科大学天津300070 天津市南开医院

目的系统评价痰热清注射液治疗全身炎症反应综合征的效果。方法检索Ovid/Pubmed、Embase、中国知网、万方数据知识服务平台、维普期刊资源整合服务平台、中国生物医学文献数据库,查找痰热清注射液治疗全身炎症反应综合征的随机对照试验... 详细信息

目的系统评价痰热清注射液治疗全身炎症反应综合征的效果。方法检索Ovid/Pubmed、Embase、中国知网、万方数据知识服务平台、维普期刊资源整合服务平台、中国生物医学文献数据库,查找痰热清注射液治疗全身炎症反应综合征的随机对照试验（RCT）,观察指标包括临床总有效率、WBC、血清C-反应蛋白（CRP）、TNF-α、血清降钙素原（PCT）、体温（T）。根据Jadad质量评分法对文献质量进行评价。结果最终纳入11篇文献,共811例患者,对照组396例、痰热清组415例。纳入文献质量总体不高,仅1篇为高质量文献。纳入文献存在发表偏倚。与对照组相比,痰热清组总有效率提高（OR=3.05,95%CI：1.46~6.39,P=0.003）,WBC下降（WMD=1.39,95%CI：2.25~0.53,P=0.002）,血清CRP（WMD=13.78,95%CI：19.96~7.60,P〈0.00 01）、PCT（SMD=2.17,95%CI：4.23 0.11,P=0.04）、TNF-α（SMD=-1.50,95%CI：2.14~0.85,P〈0.00 001）、IL-6（WMD=-24.04,95%CI：36.03~12.05,P〈0.00 01）降低,T降低（WMD=-0.66,95%CI：0.85~0.47,P〈0.00 01）。所有文献未报告痰热清注射液有不良反应。结论痰热清注射液治疗全身炎症反应综合征效果较好。

关键词：痰热清注射液全身炎症反应综合征 Meta分析

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Retinomorphic X-ray detection using perovskite with hydrionconductive organic cations

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The Innovation 2024年第4期5卷 111-117,110页

作者： Hang Yin Jincong Pang shan Zhao Haodi wu Zihao Song Xing Li Zhiping Zheng Ling Xu Jiang Tang Guangda Niu wuhan National Laboratory for Optoelectronics and School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China Optical Valley Laboratory Wuhan 430074China Institute of Microelectronics of the Chinese Academy of Sciences Beijing 100029China Research Institute of Huazhong University of science and technology in Shenzhen Shenzhen 518057China

X-ray detection is crucial across various sectors,but traditional techniques face challenges such as inefficient data transmission,redundant sensing,high power consumption,and *** innovative idea of a retinomorphic X-ray detector shows great ***,its implementation has been hindered by the absence of active layers capable of both detecting X-rays and serving as memory *** response to this critical gap,our study integrates hybrid perovskite with hydrion-conductive organic cations to develop a groundbreaking retinomorphic X-ray *** novel device stands at the nexus of technological innovation,utilizing X-ray detection,memory,and preprocessing capabilities within a single hardware *** core mechanism underlying this innovation lies in the transport of electrons and holes within the metal halide octahedral frameworks,enabling precise X-ray ***,the hydrion movement through organic cations endows the device with short-term resistive memory,facilitating rapid data processing and ***,our retinomorphic X-ray detector boasts an array of formidable features,including reconfigurable shortterm memory,a linear response curve,and an extended retention *** practical terms,this translates into the efficient capture of motion projections with minimal redundant data,achieving a compression ratio of 18.06%and an impressive recognition accuracy of up to 98.6%.In essence,our prototype represents a paradigm shift in X-ray detection *** its transformative capabilities,this retinomorphic hardware is poised to revolutionize the existing X-ray detection landscape.

关键词： perovskite conductive redundant

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β-1,3-1,4葡聚糖酶在大肠杆菌中的高效分泌表达

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食品工业科技 2015年第21期36卷 139-142,147页

作者：陈珊珊吴珊珊吴斌何冰芳南京工业大学药学院江苏南京211816

在大肠杆菌中实现β-1,3-1,4-葡聚糖酶的高效分泌表达。将实验室自主开发的信号肽ff53与β-1,3-1,4-葡聚糖酶成熟肽基因(bgl)进行融合连接到p ET-28a(+)上;通过优化诱导表达条件,28℃、8 g/L乳糖诱导10 h,重组菌*** BL21/p ET-ff53-bgl... 详细信息

在大肠杆菌中实现β-1,3-1,4-葡聚糖酶的高效分泌表达。将实验室自主开发的信号肽ff53与β-1,3-1,4-葡聚糖酶成熟肽基因(bgl)进行融合连接到p ET-28a(+)上;通过优化诱导表达条件,28℃、8 g/L乳糖诱导10 h,重组菌*** BL21/p ET-ff53-bgl发酵液上清中β-1,3-1,4-葡聚糖酶活力达到1093 U/m L,与IPTG诱导的重组菌*** BL21/p ET-bgl(583 U/m L)相比,提高了0.87倍。本研究为高密度发酵制备该酶奠定了基础。

关键词：信号肽ff53 β-1 3-1 4葡聚糖酶分泌表达大肠杆菌

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The proximity between hydroxyl and single atom determines the catalytic reactivity of Rh1/CeO_(2) single-atom catalysts

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Nano Research 2024年第1期17卷 397-406页

作者： Danfeng wu Shuyun Zhou Congcong Du Juan Li Jianyu Huang Hong-xia Shen Abhaya K.Datye shan Jiang Jeffrey T.Miller Sen Lin Haifeng Xiong State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China State Key Laboratory of Photocatalysis on Energy and Environment College of ChemistryFuzhou UniversityFuzhou 350002China Clean Nano Energy Center State Key Laboratory of Metastable Materials Science and TechnologyYanshan UniversityQinhuangdao 066004China College of Biology and Chemical Engineering Jiaxing UniversityJiaxing 314000China Department of Chemical&Biological Engineering University of New MexicoAlbuquerqueNM 8731-0001USA Davidson School of Chemical Engineering Purdue UniversityWest LafayetteIN 47907-2100USA Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM) Xiamen 361102China Fujian Key Laboratory of Rare-earth Functional Materials Fujian Shanhai Collaborative Innovation Center of Rare-earth Functional MaterialsLongyan 366300China

The local structure of the metal single-atom site is closely related to the catalytic activity of metal single-atom catalysts(SACs).However,constructing SACs with homogeneous metal active sites is a challenge due to the surface heterogeneity of the conventional ***,we prepared two Rh1/CeO_(2)SACs(0.5Rh1/r-CeO_(2)and 0.5Rh1/c-CeO_(2),respectively)using two shaped CeO_(2)(rod and cube)exposing different facets,i.e.,CeO_(2)(111)and CeO_(2)(100).In CO oxidation reaction,the T100 of 0.5Rh1/r-CeO_(2)SACs is 120°C,while the T100 of 0.5Rh1/c-CeO_(2)SACs is as high as 200°*** in-situ CO diffuse reflectance infrared Fourier transform spectroscopy(CO-DRIFTS),we found that the proximity between OH group and Rh single atom on the plane surface plays an important role in the catalytic activity of Rh1/CeO_(2)SAC system in CO *** Rh single atom trapped at the CeO_(2)(111)crystal surface forms the Rh1(OH)adjacent species,which is not found on the CeO_(2)(100)crystal surface at room ***,during CO oxidation,the OH group far from Rh single atom on the 0.5Rh1/c-CeO_(2)disappears and forms Rh1(OH)adjacent species when the temperature is above 150°*** formation of Rh1(OH)adjacentCO intermediate in the reaction is pivotal for the excellent catalytic activity,which explains the difference in the catalytic activity of Rh single atoms on two different CeO_(2)*** formed Rh1(OH)adjacent-O-Ce structure exhibits good stability in the reducing atmosphere,maintaining the Rh atomic dispersion after CO oxidation even when pre-reduced at high temperature of 500°*** functional theory(DFT)calculations validate the unique activity and reaction path of the intermediate Rh1(OH)adjacentCO species *** work demonstrates that the proximity between metal single atom and hydroxyl can determine the formation of active intermediates to affect the catalytic performances in catalysis.

关键词： crystal plane Rh1/CeO_(2)single-atom catalyst proximity of Rh and hydroxyl coordination structure CO oxidation

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