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A new luciferase immunoprecipitation system assay provided serological evidence for missed diagnosis of severe fever with thrombocytopenia syndrome

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Virologica Sinica 2022年第1期37卷 107-114页

作者： Shengyao Chen Minjun Xu Xiaoli wu Yuan Bai junming Shi Min Zhou Qiaoli wu Shuang Tang Fei Deng Bo Qin Shu Shen State Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan430071China Shaoxing Women and Children©s Hospital No.305 East Street RoadShaoxing312000China University of Chinese Academy of Sciences Beijing100049China Shaoxing People©s Hospital Zhejiang University School of MedicineShaoxing312000China

Severe fever with thrombocytopenia syndrome(SFTS),caused by SFTS virus(SFTSV)infection,was first reported in 2010 in China with an initial fatality of up to 30%.The laboratory confirmation of SFTSV infection in terms of detection of viral RNA or antibody levels is critical for SFTS diagnosis and *** this study,a new luciferase immunoprecipitation system(LIPS)assay based on p REN2 plasmid expressing SFTSV NP gene and tagged with Renilla luciferase(Rluc),was established and used to investigate the levels of antibody responses to *** 464 serum samples from febrile patients were collected in the hospital of Shaoxing City in Zhejiang Province in *** results showed that 82 of the 464 patients(17.7%)had antibody response to SFTSV,which were further supported by immunofluorescence assays(IFAs).Further,q RT-PCR and microneutralization tests showed that among the 82 positive cases,15 patients had viremia,10 patients had neutralizing antibody,and one had both(totally 26 patient).However,none of these patients were diagnosed as SFTS in the hospital probably because of their mild symptoms or subclinical *** the results indicated that at least the 26 patients having viremia or neutralizing antibody were the missed diagnosis of SFTS *** findings suggested the occurrence of SFTS and the SFTS incidence were higher than the reported level in Shaoxing in 2019,and that LIPS may provide an alternative strategy to confirm SFTSV infection in the laboratory.

关键词： Severe fever with thrombocytopenia syndrome(SFTS) Severe fever with thrombocytopenia syndrome virus(SFTSV) Luciferase immunoprecipitation systems(LIPS) Shaoxing Serological evidence Missed diagnosis

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Long-term mercury variations in tree rings of the permafrost forest,northeastern China

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Science China Earth Sciences 2022年第7期65卷 1328-1338页

作者： Huhu KANG Xiaohong LIU junming GUO Qiuliang ZHANG Yabo WANG Jie HUANG Guobao XU Guoju wu Wensen GE Shichang KANG State Key Laboratory of Cryospheric Science Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China School of Geography and Tourism Shaanxi Normal UniversityXi'an 710119China University of Chinese Academy of Sciences Beijing 100049China Forest College Inner Mongolia Agricultural UniversityHohhot 010018China Key Laboratory of Tibetan Environment Changes and Land Surface Processes Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of SciencesBeijing 100101China

Permafrost is a potential mercury(Hg)pool released by thawing,which can raise the risk of Hg pollution under global *** rings are useful archives of environment-specific Hg exposure over long *** determined Hg concentrations in tree rings of two dominant tree species(Larix gmelinii *** Pinus sylvestris ***)at permafrost sites in northeastern *** biweighted mean Hg concentrations ranged from 0.36 to 3.96 ng g^(-1) from 1840 to *** tree-ring width had no significant influence on the Hg *** Hg increased slightly before the 1970 s and peaked in the 1990 ***,the pine Hg concentration increased continuously until the 1930 s,decreased rapidly until the 1970 s,then rose to a peak in the late 1980 *** change of Hg concentrations in larch and pine revealed a time offset of 4 to 5 years,which implied possibly high mobility of Hg in pine tree *** Hg concentrations from 1920 to 1960 and subsequent decreases in isolated permafrost forests revealed the local geographical Hg cycling *** Hg concentrations and faster increases in larch suggest the role of additional winter Hg loading for the evergreen pine and species-specific differences in root absorption in response to melting *** results highlight possible geographical impacts on tree-ring Hg records,improve understanding of Hg cycles in permafrost forest,and suggest a need to sample additional species in a range of permafrost environments.

关键词： Tree rings Mercury Long-term variations Larch Pine Geographic environment Permafrost forest

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经甘草药汁炮制引入的主要活性成分助力黄药子的减毒作用及其机制研究

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中华中医药杂志 2024年第5期39卷 2369-2376页

作者：宋玲玲王君明段雅倩纪丽婕何庆文张天柱王彦嵋巫晓慧河南中医药大学药学院郑州450046 河南中医药大学呼吸疾病中医药防治省部共建协同创新中心郑州450046 豫药全产业链研发河南省协同创新中心郑州450046

目的:探究经甘草药汁炮制引入的主要活性成分甘草苷(LQ)、甘草酸(GA)对黄药子毒性成分黄独素B(DB)诱导主要毒性肝毒性的减毒作用,并初探其机制。方法:在前期发现甘草药汁制法能够对黄药子主要毒性肝毒和止咳祛痰主要功效起到减毒增效作... 详细信息

目的:探究经甘草药汁炮制引入的主要活性成分甘草苷(LQ)、甘草酸(GA)对黄药子毒性成分黄独素B(DB)诱导主要毒性肝毒性的减毒作用,并初探其机制。方法:在前期发现甘草药汁制法能够对黄药子主要毒性肝毒和止咳祛痰主要功效起到减毒增效作用且炮制减毒机制涉及引入炮制辅料药甘草汁的主要活性成分LQ和GA以及对DB含量的抑制的基础上,本研究进一步通过对DB与LQ和GA联用前后小鼠肝损伤敏感指标血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)以及肝组织病理的检测分析综合验证引入的主要活性成分LQ和GA对DB诱导的主要毒性肝毒的减毒作用。此外,围绕DB肝毒的主要机制炎症和氧化损伤,初探其减毒机制。结果:与正常组比较,DB给药致使小鼠血清ALT、AST、ALP水平均显著升高(P<0.01),肝细胞变性,并伴有炎性浸润及血管瘀血,磷酸化核转录因子κB(p-NF-κB)/环氧合酶2(COX-2)蛋白表达水平显著上调,肝磷酸化磷酯酰肌醇3激酶(p-PI3K)/蛋白激酶B(p-Akt)/核因子E2相关因子2(Nrf2)蛋白表达水平显著下调(P<0.01);而经LQ、GA干预均在不同程度上逆转了上述指标的异常(P<0.05,P<0.01)。值得注意的是,对ALT、AST水平的逆转程度分析,LQ比GA对DB诱导的肝毒性的减毒作用分别高3.3%和2.8%;对ALP水平分析,GA比LQ对DB诱导的肝毒性的减毒作用高7.1%;对炎症信号通路关键分子NF-κB、COX-2分析,LQ比GA对DB诱导肝脏炎症反应的抑制作用分别高3.2%和2.5%;对抗氧化信号通路核心分子Nrf2分析,GA比LQ对DB诱导肝脏抗氧化水平低下的上调作用高18.2%。结论:LQ和GA均能够对DB诱导的肝毒性起到减毒作用;减毒机制涉及抑制NF-κB/COX-2信号介导的肝脏炎症反应并增强转录因子Nrf2介导的抗氧化防御。

关键词：药汁活性成分引入甘草炮制黄药子成分减毒甘草苷甘草酸黄独素B

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Revealing component synergy of Ni‒Fe/black phosphorous composites synthesized by self-designed electrochemical method for enhancing photoelectrocatalytic oxygen evolution reaction

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Frontiers of Materials Science 2023年第2期17卷 143-153页

作者： He Xiao Shoufeng Xue Zimei Fu Man Zhao Li Zhang junming Zhang Haishun wu Jianfeng Jia Nianjun Yang Key Laboratory of Magnetic Molecules&Magnetic Information Materials(Ministry of Education) School of Chemistry and Materials ScienceShanxi Normal UniversityTaiyuan 030032China Department of Chemistry Hasselt University3590 DiepenbeekBelgium

Developing high-activity and low-cost catalysts is the key to eliminate the limitation of sluggish anodic oxygen evolution reaction(OER)during electrocatalytic overall water ***,Ni‒Fe/black phosphorous(BP)composites are synthesized using a simple three-electrode system,where exfoliation of bulky BP and synthesis of NiFe composites are simultaneously *** light illumination,the optimized Ni‒Fe/BP composite exhibits excellent photoelectrocatalytic OER performance(e.g.,the overpotential is 58 mV lower than a commercial RuO_(2) electrocatalyst at a current density of 10 mA·cm^(-2)).The electron transfer on this composite is proved to follow a Ni‒BP‒Fe *** electronic structure of this Ni‒Fe/BP composite is effectively regulated,leading to optimized adsorption strength of the intermediate OH*and improved intrinsic activity for the *** with active sites on the support,this Ni‒Fe/BP composite possesses abundant electrochemical active sites and a bug surface area for the *** introduction of light further accelerates the electrocatalytic *** work provides a novel and facile method to synthesize high-performance metal/BP composites as well as the approaches to reveal their OER mechanisms.

关键词： black phosphorous (photo-)electrocatalysis oxygen evolution reaction

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General approach for atomically dispersed precious metal catalysts toward hydrogen reaction

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Carbon Energy 2023年第7期5卷 100-111页

作者： Ruisong Li Daoxiong wu Peng Rao Peilin Deng Jing Li junming Luo Wei Huang Qi Chen Zhenye Kang Yijun Shen Xinlong Tian State Key Laboratory of Marine Resource Utilization in South China Sea Hainan Provincial Key Lab of Fine ChemistrySchool of Chemical Engineering and TechnologyHainan UniversityHaikouChina

As a carbon-free energy carrier,hydrogen has become the pivot for future clean energy,while efficient hydrogen production and combustion still require precious metal-based ***-atom catalysts(SACs)with high atomic utilization open up a desirable perspective for the scale applications of precious metals,but the general and facile preparation of various precious metal-based SACs remains ***,a general movable printing method has been developed to synthesize various precious metal-based SACs,such as Pd,Pt,Rh,Ir,and Ru,and the features of highly dispersed single atoms with nitrogen coordination have been identified by comprehensive *** importantly,the synthesized Pt-and Ru-based SACs exhibit much higher activities than their corresponding nanoparticle counterparts for hydrogen oxidation reaction and hydrogen evolution reaction(HER).In addition,the Pd-based SAC delivers an excellent activity for photocatalytic hydrogen *** for the superior mass activity of Ru-based SACs toward HER,density functional theory calculations confirmed that the adsorption of the hydrogen atom has a significant effect on the spin state and electronic structure of the catalysts.

关键词： hydrogen evolution reaction hydrogen oxidation reaction photocatalytic hydrogen evolution reaction precious metals single-atom catalysts

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Dayside poleward moving auroral forms and ionospheric convection under stable interplanetary magnetic field (IMF) conditions

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Science China(Technological Sciences) 2013年第4期56卷 910-916页

作者： XING ZanYang YANG HuiGen HAN DeSheng wu ZhenSen LIU junming HU ZeJun ZHANG QingHe HU HongQiao LIU YongHua School of Science Xidian University SOA Key Laboratory for Polar Science Polar Research Institute of China

Using high temporal resolution optical data obtained from three-wavelength all-sky imagers over six winters continuously at Yellow River Station （78.92°N,11.93°E） in Arctic,we statistically investigated the dependence of location of poleward moving auroral forms （PMAFs） on the interplanetary magnetic field （IMF） B z and B y components as a function of MLT and MLAT under stable IMF *** is found that more PMAFs occurred in lower latitude for Bz＆lt;0 and there was less evident IMF B y-related prenoon-postnoon asymmetry for Bz＆lt;0 than for Bz＆gt;*** found that the PMAFs were distributed over a wide range of MLT when Bz＆lt;0,which indicates that the reconnection X-line might spread like an ’S’ ***,during northward IMF,PMAFs were observed predominantly prenoon for IMF By＆gt;0 and postnoon for IMF By＆lt;0 associating with the effect of the high-latitude reconnection,which is largely consistent with the theoretical model of the convection flow.

关键词： poleward moving auroral forms (PMAFs) reconnection prenoon-postnoon asymmetry

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Luminescent ionic lattice occupation and wide tunable emission spectra of La_(2)MgZrO_(6):Bi^(3+),Eu^(3+)double perovskite phosphors for white light LED

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Journal of Rare Earths 2023年第4期41卷 489-497页

作者： Chen Yang Chenli Fan Fayaz Hussain Weiqing Sheng Kaixin Song Jun wu Qingming Huang Weitao Su junming Xu Shikuan Sun Dawei Wang College of Electronics Information Hangzhou Dianzi UniversityHangzhou 310018China Department of Materials Engineering NED University of Engineering and TechnologyMain University RoadKarachi75270Pakistan Testing Center of Fuzhou University Fuzhou UniversityFuzhou 350116China College of Sciences Hangzhou Dianzi UniversityHangzhou 310018China School of Material Science and Energy Engineering Foshan UniversityFoshan 528000China Shenzhen Institute of Advanced Electronic Materials Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesSciencesShenzhen 55China

La_(2)Mg_(1-x/2)Zr_(1-x/2)O_(6):xBi^(3+)(x=0.01-0.035,abbreviated as LMZ:Bi^(3+))and La_(2-y)Mg_(0.99)Zr_(0.99)O_(6):0.02Bi^(3+),yEu^(3+)(y=0.1-0.11,abbreviated as LMZ:Bi^(3+),Eu^(3+))double-perovskite phosphors were prepared through high-temperature solid-phase *** emission spectrum of LMZ:xBi^(3+)(x=0.01-0.035)phosphors excited at 353 nm is asymmetric in the range between 375 and 650 nm,showing strong green *** are two luminescent centers of[Mg1/Zr2-O_(6)]and[Mg2/Zr1-O_(6)]for Bi^(3+)occupation,which were analyzed through different excitation wavelengths,Gaussian fitting peaks,fluorescence decay curves and Rietveld refinement of powder X-ray diffraction *** deep study of the luminescent lattices in the LMZ matrix,the green to blue tunning-emission is observed by different excitation *** addition,red emission is obtained by co-doping Bi^(3+)/Eu^(3+),and adjustable emission was investigated by changing the content of Eu^(3+)in the co-doped phosphor formulation,so it is converted from green emission to red *** above results demonstrate how to tune emission color by co-doping rare earth ions in the double perovskite phosphor,which is attractive for future applications.

关键词： Double perovskite Phosphor Tunable emission Energy transfer Rare earths

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熵增工程在电催化反应中的研究进展

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物理化学学报 2024年第7期40卷 36-42页

作者：张新义任楷刘妍宁谷振一黄志雄郑硕航王晓彤郭晋芝 Igor V.Zatovsky 曹峻鸣吴兴隆东北师范大学化学学院长春130024 东北师范大学紫外光发光材料与技术教育部重点实验室长春130024 乌克兰国家科学院生物胶体化学研究所乌克兰基辅03142

目前对高性能与高稳定性的电催化剂进行精准合成仍然是亟待解决的问题。熵作为是最重要的热力学参数之一,是描述体系无序程度的物理量,其数值主要由材料的结构、磁矩、原子和电子振动共同决定。根据体系的构型熵值,我们通常将材料分为... 详细信息

目前对高性能与高稳定性的电催化剂进行精准合成仍然是亟待解决的问题。熵作为是最重要的热力学参数之一,是描述体系无序程度的物理量,其数值主要由材料的结构、磁矩、原子和电子振动共同决定。根据体系的构型熵值,我们通常将材料分为低熵材料(ΔS_(mix)1.5R)。随着熵值的增加,材料本征的物理与化学性质也会随之发生相应的变化。得益于不同金属元素的共存,在界面处原子级的多组分排列,所产生的协同性高熵效应能够有效地提升电催化反应的活性,因此在电催化领域中得到了广泛的研究关注。本综述对高熵电催化剂的基本概念、合成路线(“自上而下”与“自下而上”)以及在不同电催化反应类型中的高熵材料结构与性能之间的构效关系进行了系统总结,主要包括析氢(HER)、析氧(OER)、氧还原(ORR)、醇氧化(AOR)、氮还原(NRR)和二氧化碳还原反应(CO_(2)RR)等,从而阐明熵增工程对高性能电催化剂设计与应用的优势与潜力。同时,针对目前高熵催化剂研究所面临的主要问题与挑战,对未来基于熵增工程的高熵电催化剂的设计思路与合成方法进行展望。

关键词：熵增合成电化学高熵催化剂电催化

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BmCH25H,a vertebrate interferon-stimulated gene(ISG)homolog,inhibits BmNPV infection dependent on its hydroxylase activity in Bombyx mori

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Insect Science 2023年第2期30卷 321-337页

作者： Hongyun wu junming Xia Shigang Fei Yeyuan Wang Mengmeng Zhang Yiyao Guo Xian Li Luc Swevers Jingchen Sun Min Feng Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina Insect Molecular Genetics and Biotechnology National Center for Scientific Research DemokritosInstitute of Biosciences and ApplicationsAthensGreece

Cholesterol-25-hydroxylase(CH25H)has been identified as an interferon-stimulated gene(ISG)in mammals that exerts its antiviral effects by catalyzing the conversion of cholesterol to 25-hydroxycholesterol(25HC).However,invertebrates lack an antiviral system homologous to vertebrate interferons(IFNs)because the genomes of invertebrates do not encode IFN-like ***,CH25H is present in insect genomes and it therefore deserves further study of whether and by which mechanism it could exert an antiviral effect in *** this study,the Bombyx mori CH25H(BmCH25H)gene,of which the encoded protein has high homology with other lepidopteran species,was identified and located on chromosome ***,we found that the expression of BmCH25H was significantly upregulated in *** nucleopolyhedrovirus(BmNPV)-infected BmN cells and silkworm(***)larvae at the early infection *** inhibitory effect of BmCH25H on BmNPV replication was further demonstrated to depend on its catalytic residues to convert cholesterol to *** importantly,we demonstrated that during BmNPV infection,BmCH25H expression was increased through the Janus kinase–signal transducer and activator of transcription(JAK–STAT)pathway,similar to the induction of ISGs following virus infection in *** is the first report that CH25H has antiviral effects in insects;the study also elucidates the regulation of its expression and its mechanism of action.

关键词： Bombyx mori nucleopolyhedrovirus Bombyx mori Cholesterol 25-hydroxylase Interferon-stimulated genes Signal transducer and activator of transcription

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船用薄板双丝气体保护焊焊接工艺性研究

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南钢科技与管理 2018年第4期 10-12页

作者：汪晶洁田浩吴君明研究院

对南钢生产的5mm厚的船用薄板A36进行了双丝气体保护焊接工艺适用性研究。结果表明:在焊接速度达到150cm/min时,焊接热影响区最高硬度为250HV,焊接接头的综合力学性能良好,达到标准要求。

关键词：双丝气体保护焊焊接方法热影响区力学性能

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