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Intracellular recording and morphology of tectal neurons activated by contralateral nucleus isthmi in toads

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Chinese Science Bulletin 1997年第19期42卷 1647-1651页

作者： Wang, SR wu, gy Laboratory for Visual Information Processing Institute of Biophysics Chinese Academy of Sciences Beijing China

THE nucleus isthmi (NI) in lower vertebrates and its mammalian analogue, the nucleus para-bigeminalis, are all visual centres. They receive their tectal input ipsilaterally and project backipsilaterally in teleosts and birds, or bilaterally in amphibians and reptiles, as well as in mam-mals. It is well known that NI receives its visual excitation through the ipsilateral optic tec-tum (OT). NI mainly exerts inhibition on the ipsilateral OT in frogs.A recent study

关键词： nucleus isthmi tectum intracellular recording morphology toad

Subcellular localization and functional analyses of structural domains of COP1 in transgenic tobacco

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Chinese Science Bulletin 2002年第15期47卷 1250-1257,1321-1322页

作者： Wang, CX Huang, CH wu, gy wu, XY Zhao, JD Peking Univ Coll Life Sci Beijing 100871 Peoples R China

Plants have evolved an extremely exquisite light signal regulatory network to adapt to the changing ambient light conditions, in which COP1 plays a critical role of the light signal transduction. Based on the cloned pea COP1 cDNA sequence and its protein structure, four individual gene fragments encoding different structural domains of the COP1 were designed to fuse to the GFP gene. The plant expression vectors containing these fusion genes as well as the COPIGFP fusion gene were constructed and used to transform tobacco by Agribacterium as confirmed by South-]ern analyses. Antibodies were raised against the recombi-nant GFP-COP1 overproduced in Escherichia coli. 1m-munoblotting results demonstrated that all of the fusion genes were constitutively expressed in transgenic tobacco plants. We systematically investigated the different subcell-ular localization of these fusion proteins and the resulting phenotypic characteristics of these transgenic plants under light and dark conditions. Our data show that (

关键词： photomorphogenesis COP1 tobacco GFP subcellular localization.

Islet separation and islet cell culture in vitro from human embryo-pancreas

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World Journal of Gastroenterology 1999年第5期5卷 458-460页

作者： Yuan Z wu gy He YS Shao CM Zhan Y Kunming Med Coll Dept Histol & Embryol Kunming 650031 Yunnan Province Peoples R China First Affiliated Hosp Kunming Med Coll Dept Nucl Med Kunming 650032 Yunnan Province Peoples R China Kunming Med Coll Dept Biol Kunming 650031 Yunnan Province Peoples R China

INTRODUCTIONDiabetes mellitus is the most common disease and its death rate ranks the 8th in the world. Up to now, its incidence has a tendency to increase[1]. Since disorder of sugar metabolism might result in microvascular degeneration and injury of important human organs, it will endanger human health seriously. In 1969, Younszai first reported the method that could decrease diabetes symptoms by islet tissue transplantation. In 1981, our country began to treat diabetes type Ⅰ by transplanting cultured islet tissues. In our study, we digested the pieces of pancreas with collagenase and made morphological observation on islet cells cultured for 3d, 5d, 7d, 9d and 13d respectively, and the contents of insulin and C-peptide in the supernatant were detected by radioimmunoassay. The experimental model of rabbit diabetes was established, and certain curative effect was achieved in the treatment of experimental diabetes in rats.

关键词： cell culture islet cell islets of langerhans transplantetion pancreas/ embryology

Effects of Lactosucrose on intestinal barrier in TNBS-lnduced Colitis in Rats

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Effects of Lactosucrose on intestinal barrier in TNBS-lnduce...

Effect of synergistic adsorption of anion surfactants and heavy metal ions onto kaolinite surfaces

生猪优质健康养殖关键技术国际学术研讨会

作者： Z Ruan~1 YLYin~(*1 2) gy wu~2 (State Key Laboratory of Food Science and Technology Nanchang University Nanchang 330047 China Institute of Subtropical Agriculture the Chinese Academy of Sciences Changsha 410125 China)

＜正＞To investigate the effects of Lactosucrose on intestinal barrier in TNBS-Induced *** Lactosucrose were administered rats with experimental ulcerative colitis induced by 2,4,6-trinitrobenzene sulfonic acid（TNBS）.The colon structure was observed hematoxylin-eosin ***,a kind of tight junction protein,were determined by immunohistochemical *** mRNA expression was assessed by reverse transcriptase-polymerase chain reaction（RT-PCR）.Results Lactosucrose significantly improved

关键词： Lactosucrose rats

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Chinese Science Bulletin 2001年第22期46卷 1930-1932页

作者： wu, DQ Diao, gy Peng, JL Chinese Acad Sci Guangzhou Inst Geochem Guangzhou 510640 Peoples R China

The experiments were performed in solution for the synergistic adsorption of dodecylbenzene sulfonate and copper ions onto kaolinite surfaces. The results showed that the former enhances the binding constant of copper ions onto kaolinite surfaces, but copper ions deduce the binding constant of dodecylbenzene sulfonate onto kaolinite surfaces. At the same time, they depress the proton release or absorb each other during their adsorption on kaolinite surfaces.

关键词： kaolinite copper ion anion surfactant synergistic ad- sorption.

Protein synthesis and translation initiation activation in weaned piglets fed a low-protein diet supplemented with essential amino acids

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Protein synthesis and translation initiation activation in w...

Design of selective excitation pulses by using finite impulse response filtration method

2007年动物营养、保健与饲料添加剂国际学术研讨会

作者： D.Deng,W.Y.Chu,T.J.Li,R.L.Huang,K.Yao,Z.J.Liu,X.F.Kong, gy.wu and Y.L.Yin~* a Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology,Institute of Subtropical Agriculture,The Chinese Academy of Sciences P.O.Box 10,Changsha 410125

This study was conducted to determine the effects of a low-protein diet with essential amino acids（EAAs）(Lys,Met,Thr,Trp,Leu,Ile,or Val) on tissue protein fractional synthesis rate（FSR） and translation initiation factor activation in weaned *** three-line crossbred piglets（Duroc×Landrace×Yorkshine）,weaned at 21 days of age,were fed diets containing one of three crude protein（CP） levels（20.7,16.7,or 12.7%）.On d 10 of the trial all pigs were fitted with an indwelling jugular catheter for injecting L-Phe(1.5 mmol/kg body weight（BW） containing 0.6 mmol/kg BW L-[ring-H]Phe) and blood *** d 14 of the trial pigs were injected with with a flooding dose of L-Phe and tissue samples collected before all pigs were *** CP levels significantly affected（P<0.05） the FSR in pancreas, liver,kidney,and longissimus *** of eukaryotic initiation factor（eIF） 4E and ribosomal protein S6 kinase 1（S6K1） decreased with reductions in dietary CP *** liver, the association of eIF4G with eIF4E was lower,33.08%（P<0.01）,in pigs fed a 12.7%CP diet than that in pigs fed the 20.7%CP *** CP level significantly affected phosphorylation of 4E binding protein 1（4E-BP1） in longissimus muscle and liver（P<0.05）.Phosphorylation of mTOR（%total mTOR） in pigs fed the 16.7%CP and 12.7%CP diets was lower,32.97%and 31.88%)（P<0.01） respectively,than that in pigs fed the 20.7%CP diet..These results indicated that chronically feeding dietary protein to piglets lowers their known protein requirement and can reduce protein synthesis in tissues including the liver and skeletal muscle while changes in translation factor activation resulted decreased protein deposition.

关键词： Protein synthesis weaned piglets low-protein diet essential amino acids

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Chinese Science Bulletin 1997年第15期42卷 1271-1274页

作者： Li, gy Chen, SZ wu, XW Analytical Center East China Normal University Shanghai China

RECENTLY, frequency selective excitation was extensively studied and applied successfullyto various types of nuclear magnetic resonance (NMR) experiments. Solvent suppression,double resonance, multiple quantum coherence, as well as multi-dimensional NMR are all re-lated to selective excitation. Probably, its most important application is in NMR ***, selective excitation unit (SEU) has become an important accessory in modernNMR spectrometer.

关键词： nuclear magnetic resonance selective excitation finite impulse response filtration.

一氧化氮吸入治疗新生儿肺动脉高压疗效的初步评价

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新生儿科杂志 2001年第3期16卷 97-99页

作者：吴琳黄国英林其珊孙斌孙波复旦大学医学院附属儿科医院 200032

利用彩色多普勒超声心动图技术 ,评价一氧化氮 (NO)吸入治疗新生儿肺动脉高压的疗效。对 8例新生儿因肺炎、肺透明膜病、持续胎儿循环和先天性支气管肺发育不良并发呼吸衰竭而采用机械通气和NO吸入治疗。NO吸入浓度为 3～2 0ppm ,吸入... 详细信息

利用彩色多普勒超声心动图技术 ,评价一氧化氮 (NO)吸入治疗新生儿肺动脉高压的疗效。对 8例新生儿因肺炎、肺透明膜病、持续胎儿循环和先天性支气管肺发育不良并发呼吸衰竭而采用机械通气和NO吸入治疗。NO吸入浓度为 3～2 0ppm ,吸入时间为 1小时～ 35天。多普勒超声心动图利用三尖瓣返流或动脉导管未闭定量估测NO吸入前后肺动脉收缩压 (SPAP) ,同时测量上肢收缩压 (SBP) ,计算肺 /体压力 (Pp/Ps)比值。结果显示 :NO吸入 30～ 1 2 0分钟和 1 8～ 2 4小时后 ,SPAP分别由NO吸入前的 5 7± 1 1 .6mmHg降至 41± 1 0 8mmHg和 43± 1 8 2mmHg ;Pp/Ps分别由 0 87± 0 1 5降至 0 6 1± 0 1 5和 0 6 3± 0 2 1。NO吸入前后SBP无明显变化。NO吸入后卵圆孔、动脉导管和室间隔缺损水平右向左或双向分流较吸入前明显减少。结论　NO吸入治疗新生儿肺动脉高压安全有效。

关键词：一氧化氮肺动脉高压新生儿治疗

Progress and trend of sensor technology for on-line oil monitoring

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Science China(Technological Sciences) 2013年第12期56卷 2914-2926页

作者： wu TongHai wu HongKun DU Ying PENG ZhongXiao Key Laboratory of Modern Design and Rotor Bearing System of Ministry Xi’an Jiaotong University School of Mechanical and Manufacturing Engineering the University of New South Wales Sydney (2052) Australia

Oil monitoring constitutes an important and essential component of condition monitoring technologies and has distinguished advantages in revealing wear,lubrication and friction conditions of *** developed on-line/in-line oil monitoring technologies extend the merits into real-time applications and demonstrate significant benefits in maintenance and management of *** paper comprehensively reviews the progress of on-line/in-line oil monitoring techniques including sensor technologies,their scopes and industrial *** on the existing developments and applications of the sensors for oil quality and wear debris measurements,the trends for future sensor developments are discussed with focuses on accurate,integrated and intelligent features along with exploring a fundamental issue,that is,acquiring the knowledge on degradation mechanisms which has not received sufficient attention until *** status of applications of on-line oil monitoring is also *** limited reports have been found on this topic,increasing awareness and encouraging progress in on-line monitoring techniques are recognized in applications such as aircraft,shipping,railway,mining,*** fundamental issues for further extending the on-line oil monitoring techniques in industries are proposed and they include long-term reliability of sensors in harsh conditions,and agreement with fault or maintenance determination.

关键词： on-line oil monitoring sensor condition based monitoring oil analysis