检索结果-南通市图书馆

Biomimetic“Gemini nanoimmunoregulators”orchestrated for boosted photoimmunotherapy by spatiotemporally modulating PD-L1 and tumor-associated macrophages

引用

Acta Pharmaceutica Sinica B 2024年第3期14卷 1345-1361页

作者： Honglin Huang Ningxi Li Xiaodan Wei Qingzhi Li Junhan Guo Geng Yang Hong Yang Lulu Cai Yiyao Liu chunhui wu Department of Pharmacy Personalized Drug Therapy Key Laboratory of Sichuan ProvinceSichuan Provincial People’s Hospitaland School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengdu 610054China TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610072China

A novel strategy of not only stimulating the immune cycle but also modulating the immunosuppressive tumor microenvironment is of vital importance to efficient cancer ***,a new type of spatiotemporal biomimetic“Gemini nanoimmunoregulators”was engineered to activate robust systemic photoimmunotherapy by integrating the triple-punch of amplified immunogenic cell death(ICD),tumor-associated macrophages(TAMs)phenotype reprogramming and programmed cell death ligand 1(PD-L1)***“Gemini nanoimmunoregulators”PM@RM-T7 and PR@RM-M2 were constructed by taking the biocompatible mesoporous polydopamine(mPDA)as nanovectors to deliver metformin(Met)and toll-like receptor 7/8 agonist resiquimod(R848)to cancer cells and TAMs by specific biorecognition via wrapping of red blood cell membrane(RM)inlaid with T7or M2 ***/Met@RM-T7(abbreviated as PM@RM-T7)was constructed to elicit an amplified in situ ICD effect through the targeted PTT and effectively stimulated the anticancer ***,PD-L1 on the remaining cancer cells was degraded by the burst metformin to prevent immune ***,mPDA/R848@RM-M2(abbreviated as PR@RM-M2)specifically recognized TAMs and reset the phenotype from M2 to M1 state,thus disrupting the immunosuppressive microenvironment and further boosting the function of cytotoxic T *** pair of sister nanoimmunoregulators cooperatively orchestrated the comprehensive anticancer activity,which remarkably inhibited the growth of primary and distant 4T1 tumors and prevented malignant *** study highlights the spatiotemporal cooperative modalities using multiple nanomedicines and provides a new paradigm for efficient cancer immunotherapy against metastatic-prone tumors.

关键词： Biomimetic immunoregulator Spatiotemporal delivery Targeted photothermal therapy Amplified immunogenic cell death Immunosuppressive tumor microenvironment PD-L1 degradation TAMs phenotype reversion Metastasis inhibition

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gray code based gradient-free optimization algorithm for parameterized quantum circuit

引用

Chinese Physics B 2024年第2期33卷 189-194页

作者：张安琪武春辉赵生妹 Institute of Signal Processing and Transmission Nanjing University of Posts and TelecommunicationsNanjing 210003China Key Laboratory of Broadband Wireless Communication and Sensor Network Technology(Ministry of Education) Nanjing University of Posts and TelecommunicationsNanjing 210003China

A Gray code based gradient-free optimization(GCO)algorithm is proposed to update the parameters of parameterized quantum circuits(PQCs)in this *** parameter of PQCs is encoded as a binary string,named as a gene,and a genetic-based method is adopted to select the *** individuals in the offspring are decoded in Gray code way to keep Hamming distance,and then are evaluated to obtain the best one with the lowest cost value in each *** algorithm is performed iteratively for all parameters one by one until the cost value satisfies the stop condition or the number of iterations is *** GCO algorithm is demonstrated for classification tasks in Iris and MNIST datasets,and their performance are compared by those with the Bayesian optimization algorithm and binary code based optimization *** simulation results show that the GCO algorithm can reach high accuracies steadily for quantum classification ***,the GCO algorithm has a robust performance in the noise environment.

关键词： gradient-free optimization Gray code genetic-based method

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于计算思维的任务驱动教学法研究——以“编译原理”为例

引用

无线互联科技 2024年第11期21卷 93-96页

作者：陈洪生吴亮吴春辉湖北科技学院湖北咸宁437100 湖北省通城县第一中学湖北通城437400

计算思维是以计算能力和思维能力为基础的一种思维方式。它是每个人需要具备的基本技能,也是人类求解问题的一条途径。任务驱动教学法就是将课程的教学内容设置成与其紧密结合而富有趣味性和挑战性的任务。文章介绍的教学法就是将计算... 详细信息

计算思维是以计算能力和思维能力为基础的一种思维方式。它是每个人需要具备的基本技能,也是人类求解问题的一条途径。任务驱动教学法就是将课程的教学内容设置成与其紧密结合而富有趣味性和挑战性的任务。文章介绍的教学法就是将计算思维和任务驱动教学法相结合。它不但可以更好地培养学生的思维模式,激发学生的学习积极性,还能达到较好的教学效果。

关键词：计算思维思维方式任务驱动式编译原理课程

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

恩格列净通过转录因子EB调控溶酶体自噬改善人原代脐静脉内皮细胞损伤

引用

温州医科大学学报 2024年第7期54卷 517-527页

作者：吴春慧吴慧洋吴懋牛超朱进顺褚茂平温州医科大学附属第二医院儿童心血管内科浙江温州325027 温州医科大学附属第一医院肿瘤内科浙江温州325015

目的:探究恩格列净(EMPA)改善人原代脐静脉内皮细胞(HUVECs)内皮损伤的机制。方法:用TNFα对HUVECs进行刺激,分为5组:①对照组:PBS处理;②TNFα组:TNFα刺激;③EMPA组:TNFα+EMPA;④BAF组:巴夫洛霉素A1(BafA1)+TNFα+EMPA;⑤siTFEB组:细胞造模前预干扰转录因子EB(TFEB),余同EMPA组。造模24h后收集细胞。分别进行Westernblot检测血管内皮钙黏蛋白(VE-cadherin)、细胞间黏附分子(ICAM)、血管细胞黏附分子(VCAM)、溶酶体相关膜糖蛋白1(LAMP1)、P62、LC3、TFEB、pTFEB等蛋白的表达情况;细胞免疫荧光检测血管内皮VE-cadherin、LAMP1、TFEB等指标;单核细胞黏附、细胞渗漏、RNA测序检测、腺病毒转染GFP-LC3检测细胞内的LC3堆积量;慢病毒转染RFP-GFP-LC3检测细胞内的自噬流。结果:Westernblot结果显示,TNFα组较对照组的P62、LC3、pTFEB表达水平升高(P<0.01),LAMP1、TFEB下降(P<0.01);EMPA组较TNFα组P62、LC3、pTFEB表达水平下降(P<0.05),LAMP1、TFEB升高(P<0.05);BAF组较EMPA组的P62、LC3表达量升高(P<0.01);干扰TFEB后,siTFEB组较EMPA组的P62、LC3、表达量升高(P<0.01),LAMP1下降(P<0.01),免疫荧光结果显示TNFα组较对照组的TFEB在胞质堆积增加(P<0.01),EMPA组较TNFα组TFEB在胞质堆积减少(P<0.01),RNA测结果提示EMPA激活自噬通路;RFP-GFP-LC3双荧光结果显示EMPA组较TNFα组自噬溶酶体形成增多(P<0.01)。结论:EMPA通过TFEB增强溶酶体自噬,改善HUVECs内皮损伤。

关键词：川崎病人脐静脉内皮细胞自噬内皮细胞损伤转录因子EB 钠-葡萄糖共转运体2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhanced in vitro anticancer activity of quercetin mediated by functionalized CdTe QDs

引用

Science China Chemistry 2014年第11期57卷 1579-1588页

作者： wu chunhui SHI LiXin wu ChangYu GUO DaDong SELKE Matthias WANG XueMei School of Life Science and Technology University of Electronic Science and Technology of China State Key Laboratory of Bioelectronics (Chien-Shiung wu Laboratory) Southeast University Department of Chemistry and Biochemistry California State University

We report in this study the effects of red-emitting CdTe QDs capped with cysteamine(Cys-CdTe) on the in vitro anticancer activity of the well-known flavenoid quercetin(Qu). Various techniques, including the methylthiazolyldiphenyl-tetrazolium bromide assay, the real-time cell electronic sensing system, the optical and fluorescence imaging, and electrochemical methods have been utilized to study the potential interactions of Cys-CdTe QDs with Qu. The observations demonstrate that the safe-dosage Cys-CdTe QDs can greatly improve the drug uptake and enhance the inhibition efficiency of Qu towards the proliferation of cancer cells such as HepG2 cells. This study implies that Cys-CdTe QDs may be used for cancer therapy and that they exert a synergic anticancer effect when bound to drug molecules.

关键词： quantum dots quercetin nanocomposites cancer cell inhibition

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bifunctional Shared Fibers for High‑Efficiency Self‑Powered Fiber‑Shaped Photocapacitors

引用

Advanced Fiber Materials 2023年第1期5卷 130-137页

作者： Chuan He Jianli Cheng chunhui wu Bin Wang Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 611731China Yangtze Delta Region Institute(Huzhou) University of Electronic Science and Technology of ChinaHuzhou 313001China Institute of Chemical Materials China Academy of Engineering PhysicsChengdu 621900China

Fiber-shaped photocapacitors(FPCs)based on shared bifunctional fiber electrodes for supercapacitors and solar cells hold great potential for the realization of self-powered systems for flexible wearable ***,the reported electrodes for FPCs still face certain limitations,such as limited specific energy density,low total photochemical–electric energy conver-sion efficiency(ηtotal),and poor ***,hollow fibers consisting of partially reduced graphene oxide and a highly conductive polymer are assembled by wet-spinning and employed as shared bifunctional fibers to fabricate self-powered ***,the FPCs achieve high flexibility and aηtotal of 4.2%.This study illustrates a feasible way to design high-performance FPCs and their applications in flexible electronics.

关键词： Photocapacitors Fiber electrodes Supercapacitors Solar cells Total efficiency

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

GaN-based LEDs for light communication

引用

Science China(Physics,Mechanics & Astronomy) 2016年第10期59卷 1-10页

作者： LiXia Zhao ShiChao Zhu chunhui wu Chao Yang ZhiGuo Yu Hua Yang Lei Liu Semiconductor Lighting Research and Development Center Institute of Semiconductors Chinese Academy of Sciences

Rapid improvement in the efficiency of GaN-based LEDs not only speed up its applications for general illumination, but offer the possibilities for data transmission. This review is to provide an overview of current progresses of GaN-based LEDs for light communications. The modulation bandwidth of GaN-based LEDs has been first improved by optimizing the LED epilayer structures and the modulation bandwidth of 73 MHz was achieved at the driving current density of 40 A/cm2 by changing the multi-quantum well structures. After that, in order to increase the current density tolerance, different parallel flip-chip micro-LED arrays were fabricated. With a high injected current density of ~7900 A/cm2, a maximum modulation bandwidth of ~227 MHz was obtained with optical power greater than 30 mW. Besides the increase of carrier concentrations, the radiative recombination coefficient B was also enhanced by modifying the photon surrounding environment based on some novel nanostructures such as resonant cavity, surface plasmon, and photonic crystals. The optical 3 dB modulation bandwidth of GaN-based nanostructure LEDs with Ag nanoparticles was enhanced by 2 times compared with GaN-based nanostructure LEDs without Ag *** results demonstrate that using the QW-SP coupling can effectively help to enhance the carrier spontaneous emission rate and also increase the modulation bandwidth for LEDs, especially for LEDs with high intrinsic IQE. In addition, we discuss the progress of the faster color conversion stimulated by GaN-based LEDs.

关键词： GaN-based LEDs modulation bandwidth carrier concentration radiative recombination coefficient

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High efficiency of protoplast preparation for artificially cultured Ulva prolifera(Ulvophyceae,Chlorophyta)

引用

Journal of Oceanology and Limnology 2018年第5期36卷 1806-1811页

作者： wu chunhui JIANG Peng ZHAO Jin FU Huihui Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China University of Chinese Academy of Sciences Beijing 100049China

Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither F_v/F_m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an ef fective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast((31.5±1.9)×10~6 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.

关键词： protoplast Ulva prolifera regeneration artificial culture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Homogentisic acid γ-lactone suppresses the virulence factors of Pseudomonas aeruginosa by quenching its quorum sensing signal molecules

引用

Chinese Chemical Letters 2018年第2期29卷 313-316页

作者： Shuihong Li Polly H.M. Leung Xinya Xu chunhui wu Department of Health Technology and Informatics The Hong Kong Polytechnic University Hong Kong China Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study University of South China Hengyang 421001 China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510201 Chino Center for Informational Biology University of Electronic Science and Technology of China Chengdu 610054 China

Bacterial quorum sensing（QS） molecules are involved in the coordination of certain behaviors such as biofilm formation, virulence and antibiotic resistance. QS molecules（autoinducers） and their corresponding receptors have been recognized as important therapeutic targets for drug-resistant infections and biofilm-associated infections（BAI）. This study assessed the multiple biological effects of homogentisic acid y-lactone（HgAL）, a furanone derivative. The anti-QS and anti-biofilm effects of HgAL against PAO1 strain were evaluated using CLSM, SEM, HPLC and other biochemical methods The results showed that HgAL could effectively inhibit the production of pyocyanin and extracellular matrix, as well as reduce the adherence ability and biofilm formation of Pseudomonas aeruginosa. Inhibition of virulence is attributed to the suppressive effect of HgAL on biosynthesis of 3-oxo-C12-HSLand C4-HSL（two kinds of QS signaling molecules in P. aeruginosa）. Our results support HgAL as a potential agent for prevention of BAI in the healthcare settings.

关键词： Homogentisicacid γ-lactone Quorum sensing Biofilm Pseudomonas aeruginosa Pyocyanin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhanced capacitive energy storage and dielectric temperature stability of A-site disordered high-entropy perovskite oxides

引用

Journal of Materials Science & Technology 2023年第14期145卷 66-73页

作者： Yating Ning Yongping Pu chunhui wu Shiyu Zhou Lei Zhang Jinbo Zhang Xian Zhang Yangchao Shang School of Materials Science and Engineering Shaanxi University of Science&TechnologyXi’an 710021China School of Electronic Information and Artificial Intelligence Shaanxi University of Science and TechnologyXi’an 710021China

In this work,a novel high entropy perovskite oxide(1-x)(Na_(0.2)Bi_(0.2)Ba_(0.2)Sr_(0.2)Ca_(0.2))TiO_(3-x)NaNbO_(3)(abbrevi-ated as(1-x)NBBSCT-x NN,x=0,0.05,0.1,0.15,and 0.2)was designed to improve temperature dielectric stability and energy storage performance by combining relaxor and antiferroelectric *** optimal composition of x=0.2 exhibits a high energy storage density of 3.51 J/cm^(3),together with wide temperature stable stability(■<15%,-70 to 110℃),excellent frequency stability(W rec andηvary by only±2.1%and±5.2%within the range of 1-600 Hz)and fast discharge rate(t_(0.9)=55.2 ns).This is mainly due to the enhancement of relaxation behavior and increase of E b caused by the decrease of grain *** results offer a new strategy for designing high entropy ceramic materials of high performance in the future.

关键词： Energy storage High-entropy perovskite Temperature stability Relaxor ferroelectrics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：