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Chinese Journal of Chemical Engineering 2024年第1期65卷 276-285页

作者： yin wu Yan Sun Department of Biochemical Engineering School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education)Tianjin UniversityTianjin 300350China

Biocatalysis in organic solvents(OSs)has numerous important applications,but native enzymes in OSs often exhibit limited catalytic ***,we proposed a computation-aided surface charge engineering strategy to improve the catalytic performance of haloalkane dehalogenase DhaA in OSs based on the energetic analysis of substrate binding to the DhaA *** variants with enhanced OS resistance were obtained by replacing negative charged residues on the surface with positive charged residue(Arg).Particularly,a four-substitution variant E16R/E93R/E121R/E257R exhibited the best catalytic performance(five-fold improvement in OS resistance and seven-fold half-life increase in 40%(vol)dimethylsulfoxide).As a result,the overall catalytic performance of the variant could be at least 26 times higher than the wild-type *** spectroscopy and molecular dynamics simulation studies revealed that the residue substitution mainly enhanced OS resistance from four aspects:(a)improved the overall structural stability,(b)increased the hydrophobicity of the local microenvironment around the catalytic triad,(c)enriched the hydrophobic substrate around the enzyme molecule,and(d)lowered the contact frequency between OS molecules and the catalytic *** findings validate that computationaided surface charge engineering is an effective and ingenious rational strategy for tailoring enzyme performance in OSs.

关键词： Surface charge engineering Organic solvent resistance Molecular dynamics simulation Haloalkane dehalogenase

LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus

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Zoological Research 2024年第1期45卷 25-35页

作者： Xian-Hui Ning Bing Han Ye Peng Shao-wu yin College of Marine Science and Engineering Nanjing Normal UniversityNanjingJiangsu 210023China CAS Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdaoShandong 266071China Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province LianyungangJiangsu 222005China

Long non-coding RNAs(lncRNAs)function as key modulators in mammalian immunity,particularly due to their involvement in lncRNA-mediated competitive endogenous RNA(ceRNA)*** their recognized significance in mammals,research on lncRNAs in lower vertebrates remains *** the present study,we characterized the first immune-related lncRNA(pol-lnc78)in the teleost Japanese flounder(Paralichthys olivaceus).Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein(SARM),the fifth discovered member of the Toll/interleukin 1(IL-1)receptor(TIR)adaptor *** ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor(TLR)signaling ***,SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1βand tumor necrosis factor-α(TNF-α).Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3’untranslated region(UTR),thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial ***,pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM *** infection,the negative regulators pol-lnc78 and SARM were significantly down-regulated,while pol-miR-n199-3p was significantly up-regulated,thus favoring host antibacterial *** findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.

关键词： LncRNA SARM miRNA ceRNA Antibacterial response

Red blood cell membrane-coated FLT3 inhibitor nanoparticles to enhance FLT3-ITD acute myeloid leukemia treatment

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Chinese Chemical Letters 2024年第9期35卷 303-308页

作者： Jisheng Liu Junli Chen Xifeng Zhang yin wu Xin Qi Jie Wang Xiang Gao Department of Neurosurgery and Institute of Neurosurgery State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalWest China Medical SchoolSichuan UniversityChengdu 610041China West China School of Basic Medical Sciences&Forensic Medicine Sichuan UniversityChengdu 610041China Department of Hematology West China HospitalSichuan UniversityChengdu 610041China

FMS-like tyrosine kinase 3(FLT3)is a viable and important therapeutic target for acute myeloid leukemia(AML).FLT3 internal tandem duplication(FLT3-ITD)mutations have been identified in approximately 30%of AML patients,and are associated with unfavorable prognosis,higher risk of relapse,drug resistance,and poor clinical *** FLT3 inhibitors have demonstrated promising efficacy,they cannot cure AML or even significantly extend the lives of patients with FLT3-ITD *** is partly because of poor water solubility,insufficient membrane penetration and short half-life of small molecule ***,the presence of enzymes like CYP3A4 in bone marrow accelerate the elimination and metabolism of FLT3 inhibitors,resulting in low plasma concentrations and side *** we report the erythrocyte membrane-camouflaged FLT3 inhibitor nanoparticles to enhance FLT3-ITD AML ***,we physically coextruded red blood cell(RBC)membrane vesicles with nanoparticles derived from FLT3 inhibitor F30 to obtain F30@RBC-M,which exhibited comparable potent FLT3-ITD inhibitory effects compared to free F30 in vitro,while displaying a higher potent antitumor efficacy in xenograft models due to the prolonged circulation ***,administration of F30@RBC-M significantly extended the survival of mice in a transplanted mouse model than F30 free *** findings suggest that RBC membrane-coated nanoparticles derived from FLT3 inhibitors hold promise as a tool to enhance the therapeutic efficacy to treat FLT3-ITD AML.

关键词： FLT3-ITD Acute myeloid leukemia AML Red blood cell membrane Biomimetics

Spatiotemporal evolution and future simulation of land use/land cover in the Turpan-Hami Basin,China

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Journal of Arid Land 2024年第10期16卷 1303-1326页

作者： CHEN Yiyang ZHANG Li YAN Min wu yin DONG Yuqi SHAO Wei ZHANG Qinglan Key Laboratory of Digital Earth Science Aerospace Information Research InstituteChinese Academy of SciencesBeijing100094China International Research Center of Big Data for Sustainable Development Goals Beijing 100094China University of Chinese Academy of Sciences Beijing 100094China Xinjiang Uygur Autonomous Region Natural Resources Planning and Research Institute Urumqi 830011China School of Geomatics and Marine Information Jiangsu Ocean UniversityLianyungang 222001China College of Geomatics and Geoinformation Guilin University of TechnologyGuilin 541004China

The Turpan-Hami(Tuha)Basin in Xinjiang Uygur Autonomous Region of China,holds significant strategic importance as a key economic artery of the ancient Silk Road and the Belt and Road Initiative,necessitating a holistic understanding of the spatiotemporal evolution of land use/land cover(LULC)to foster sustainable planning that is tailored to the region's unique resource ***,existing LULC classification methods demonstrate inadequate accuracy,hindering effective regional *** this study,we established a two-level LULC classification system(8 primary types and 22 secondary types)for the Tuha *** employing Landsat 5/7/8 imagery at 5-a intervals,we developed the LULC dataset of the Tuha Basin from 1990 to 2020,conducted the accuracy assessment and spatiotemporal evolution analysis,and simulated the future LULC under various scenarios via the Markov-Future Land Use Simulation(Markov-FLUS)*** results revealed that the average overall accuracy values of our LULC dataset were 0.917 and 0.864 for the primary types and secondary types,*** with the seven mainstream LULC products(GlobeLand30,Global 30-meter Land Cover with Fine Classification System(GLC_FCS30),Finer Resolution Observation and Monitoring of Global Land Cover PLUS(FROM_GLC PLUS),ESA Global Land Cover(ESA_LC),Esri Land Cover(ESRI_LC),China Multi-Period Land Use Land Cover Change Remote Sensing Monitoring Dataset(CNLUCC),and China Annual Land Cover Dataset(CLCD))in 2020,our LULC data exhibited dramatically elevated overall accuracy and provided more precise delineations for land features,thereby yielding high-quality data backups for land resource analyses within the *** 2020,unused land(78.0%of the study area)and grassland(18.6%)were the dominant LULC types of the basin;although cropland and construction land constituted less than 1.0%of the total area,they played a vital role in arid land development and primarily situated within oases that form the urban co

关键词： land use/land cover(LULC) future simulation manual interpretation Markov-Future Land Use Simulation(Markov-FLUS)model Turpan-Hami(Tuha)Basin Xinjiang

Pneumocephalus Following Combined Spinal-Epidural Anesthesia: A Case Report Analysis

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Case Reports in Clinical Medicine 2024年第9期13卷 418-424页

作者： Tao Li Xiaoqin Zeng yin wu Department of Anaesthesiology The Second Peoples Hospital of Jingzhou Jingzhou China

Background: Combined spinal-epidural anesthesia (CSEA) is widely used in clinical anesthesia due to its rapid onset, reliable anesthetic effect, and strong controllability. Although advancements in technique have reduced the frequency and severity of common complications, reports of rare and serious complications such as pneumocephalus, remain scarce. Case Report: This article presents a case of pneumocephalus following CSEA in a middle-aged female patient undergoing surgery for an intrauterine space-occupying lesion. The patient experienced severe headache postoperatively, and imaging confirmed the presence of intracranial air. After receiving active symptomatic treatment, the patient recovered and was discharged. Conclusion: This case underscores the importance of adhering to standard anesthesia protocols and increasing awareness of rare CSEA complications, particularly the risk of pneumocephalus. Early recognition and timely management are crucial. There is a need to further enhance training and research in anesthetic procedures to improve clinical anesthesia quality and ensure patient safety.

关键词： Combined Spinal-Epidural Anesthesia Pneumocephalus Intrauterine Space-Occupying Lesion Anesthesia Complications Clinical Anesthesia Quality

基于CTGAN与GDMPA-RF算法的活立木含水率诊断方法优化研究

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传感技术学报 2024年第6期37卷 1025-1034页

作者：杨能飞吴寅南京林业大学信息科学技术学院江苏南京210037

活立木含水率的精准实时诊断是智慧林业领域的重要研究内容,其可为植物生理状态分析、林区生态水文调控、林火预警防范等做出关键指征。基于无线声发射传感器网络(Wireless Acoustic Sensor Network,WASN)系统的含水率测定方法既可实现... 详细信息

活立木含水率的精准实时诊断是智慧林业领域的重要研究内容,其可为植物生理状态分析、林区生态水文调控、林火预警防范等做出关键指征。基于无线声发射传感器网络(Wireless Acoustic Sensor Network,WASN)系统的含水率测定方法既可实现高效无损探测,又能长期野外部署,尤为适合林场实际需求。为了进一步提升WASN的辨识准确率,首先利用条件表格生成对抗网络(Conditional Tabular GAN,CTGAN)对所采集的AE特征进行数据增广,其次基于分布式梯度提升框架(Light Gradient Boosting Machine,LightGBM)对扩增后的混合数据集进行特征优选,然后提出了黄金正弦动态海洋捕食者算法优化的随机森林(Golden-Sine Dynamic Marine Predators Algorithm-Random Forests,GDMPA-RF)策略,并以此建立含水率精准反演模型。实验对比结果显示,基于优选特征子集构建的GDMPA-RF模型在立木含水率诊断性能强化方面效果最佳,其准确率(Accuracy)、精确率(Precision)、F1分数(F1-Score)、加权平均(Weighted Average)和AUC分别为99.17%、99.52%、98.14%、0.9943和0.9850,均高于鲸鱼优化算法等结合RF模型的评估指标,说明方法具有优良的监测效能,较好地优化了活立木树干含水率的在线实时推演精度。

关键词：无线声发射传感器网络活立木含水率条件表格生成对抗网络黄金正弦动态海洋捕食者算法随机森林

肿瘤靶向免疫治疗时代难点问题及中医药切入点

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中国中西医结合杂志 2024年第8期44卷 901-905页

作者：吴万垠广东省中医院肿瘤科广州510120

近20年来,随着现代临床肿瘤学分子靶向药物及免疫药物的陆续问世,使得各期恶性肿瘤的总体疗效均得到了大幅度的提高,特别是在晚期恶性肿瘤的药物治疗方面,已由20世纪90年代单纯的以化学药物治疗为主的模式,转变为靶向免疫药物与化疗药... 详细信息

近20年来,随着现代临床肿瘤学分子靶向药物及免疫药物的陆续问世,使得各期恶性肿瘤的总体疗效均得到了大幅度的提高,特别是在晚期恶性肿瘤的药物治疗方面,已由20世纪90年代单纯的以化学药物治疗为主的模式,转变为靶向免疫药物与化疗药物鼎立甚至超越的模式。

关键词：化学药物治疗临床肿瘤学分子靶向药物晚期恶性肿瘤化疗药物中医药免疫药物难点问题

荧光原位杂交扩增探针的结构光照明超分辨成像与计数

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激光与光电子学进展 2024年第4期61卷 183-188页

作者：吴寅梁永张洁李辉中国科学技术大学生物医学工程学院(苏州) 生命科学与医学部江苏苏州215163 中国科学院苏州生物医学工程技术研究所江苏省医用光学重点实验室江苏苏州215163

人类表皮生长因子受体-2(HER2)的异常扩增会导致癌细胞的过度增殖和肿瘤恶化。在采用常规光学显微成像技术检测扩增水平较高的乳腺癌细胞HER2基因时,荧光原位杂交探针的荧光信号斑点呈簇状分布,难以精确计数。应用结构光照明超分辨成像... 详细信息

人类表皮生长因子受体-2(HER2)的异常扩增会导致癌细胞的过度增殖和肿瘤恶化。在采用常规光学显微成像技术检测扩增水平较高的乳腺癌细胞HER2基因时,荧光原位杂交探针的荧光信号斑点呈簇状分布,难以精确计数。应用结构光照明超分辨成像技术对HER2基因荧光原位杂交的病理切片进行成像,从而分辨距离较近的荧光探针。通过大视场扫描成像和图像拼接,对数百个细胞进行成像和统计分析,提高了高扩增水平病理切片上HER2探针计数的准确性。

关键词：乳腺癌病理诊断荧光原位杂交结构光照明超分辨成像图像拼接

跨行政区域公路交通的一体化对区域经济发展的影响

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公路 2024年第4期69卷 208-215页

作者：吴寅恺王虹安徽省社会科学院区域现代化研究院合肥市230053

跨行政区域公路交通的一体化是推进区域一体化发展的基础和先导。基于对长三角省际毗邻县域地区公路交通建设情况的分析,构建多期DID模型,研究了跨行政区域公路交通一体化对省际毗邻地区经济发展的影响效应。结果显示,省际毗邻县域地区... 详细信息

跨行政区域公路交通的一体化是推进区域一体化发展的基础和先导。基于对长三角省际毗邻县域地区公路交通建设情况的分析,构建多期DID模型,研究了跨行政区域公路交通一体化对省际毗邻地区经济发展的影响效应。结果显示,省际毗邻县域地区公路交通一体化为地区经济发展带来了“水平效应”,却没有带来“增长效应”。同时,公路交通一体化会促进省际毗邻地区间人口的流动,但在短期内却无法优化地区的产业结构。此外,相较于发达地区,公路交通一体化能够通过“扩散效应”让发达地区带动欠发达地区经济的发展。为此,建议要进一步加大省际毗邻地区公路基础设施的路网密度,以公路交通一体化带动欠发达地区的经济发展,以实现省际毗邻地区间的协调发展。

关键词：公路交通一体化省际毗邻地区经济发展多期DID模型

以金融科技创新和高质量发展全面推进金融强国建设

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中国井冈山干部学院学报 2024年第3期17卷 81-90页

作者：吴垠西南财经大学经济学院四川成都611130

金融是现代经济的核心,是国家发展的重要核心竞争力,是促进经济高质量发展的重要一环。在新的历史阶段,系统性纵深推进科技创新,使其与金融领域结合并全面深化拓展,更好地赋能金融科技,是我们深入实施创新驱动发展战略、规避金融领域各... 详细信息

金融是现代经济的核心,是国家发展的重要核心竞争力,是促进经济高质量发展的重要一环。在新的历史阶段,系统性纵深推进科技创新,使其与金融领域结合并全面深化拓展,更好地赋能金融科技,是我们深入实施创新驱动发展战略、规避金融领域各种风险、建设现代化金融科技强国的必由之路。立足于新发展阶段,我们要坚持以人民为中心的发展思想,坚定不移地贯彻新发展理念,全方位落实金融科技创新对高质量发展的积极作用。尤其是面对中国式现代化发展的战略机遇、战略挑战和目标要求,需要立足于新发展阶段在金融领域实现新的突破,更好地实现金融高质量发展,以金融赋能全面推进金融强国建设。

关键词：金融科技创新金融高质量发展中国式现代化金融强国