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A CRISPR/RfxC as13d-mediated strategy for efficient RNA knockdown in mouse embryonic development

Science China Life Sciences 2024年

作者： Lin Zhang Shi-Meng Cao Hao wu Meng Yan Jinsong Li ling-ling Chen State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular Andrology Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of RNA Innovation Science and Engineering CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology University of Chinese Academy of Sciences Chinese Academy of Sciences Key Laboratory of Systems Health Science of Zhejiang Province School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences School of Life Science and Technology ShanghaiTech University New Cornerstone Science Laboratory Shenzhen

The growing variety of RNA classes, such as mRNAs, lncRNAs, and circRNAs, plays pivotal roles in both developmental processes and various pathophysiological conditions. Nonetheless, our comprehension of RNA functions in live organisms remains limited due to the absence of durable and effective strategies for directly influencing RNA levels. In this study, we combined the CRISPR-RfxCas13d system with spermlike stem cell-mediated semi-cloning techniques, which enabled the suppressed expression of different RNA species. This approach was employed to interfere with the expression of three types of RNA molecules: Sfmbt2 mRNA, Fendrr lncRNA, and circMan1a2(2,3,4,5,6). The results confirmed the critical roles of these RNAs in embryonic development, as their loss led to observable phenotypes, including embryonic lethality, delayed embryonic development, and embryo resorption. In summary, our methodology offers a potent toolkit for silencing specific RNA targets in living organisms without introducing genetic alterations.

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超重伴腹型肥胖的正常高值血压者的动脉僵硬度变化及相关影响因素

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中华高血压杂志 2020年第1期28卷 65-69页

作者：王鲁雁孙宁玲吴云涛吴寿岭刘小云北京大学人民医院心脏中心北京100044 开滦总医院心内科

目的了解超重合并腹型肥胖正常高值血压人群大动脉僵硬度的变化及其影响因素。方法入选体质量指数(BMI)≥24.0 kg/m^2同时腹围增加(男性≥90,女性≥85 cm),收缩压介于120~139 mm Hg或舒张压80~89 mm Hg者为超重腹型肥胖正常高值血压组(n... 详细信息

目的了解超重合并腹型肥胖正常高值血压人群大动脉僵硬度的变化及其影响因素。方法入选体质量指数(BMI)≥24.0 kg/m^2同时腹围增加(男性≥90,女性≥85 cm),收缩压介于120~139 mm Hg或舒张压80~89 mm Hg者为超重腹型肥胖正常高值血压组(n=87),同时入选BMI<24.0 kg/m^2、血压<120/80 mm Hg无疾病者为对照组(n=87)。所有入选者进行颈股动脉脉搏波传导速度(cfPWV)检测;同时留取清晨空腹血清测定血糖、血脂;并应用酶联免疫吸附试验(ELISA)法进行血清细胞间黏附分子-1(ICAM1)、单核细胞趋化蛋白-1(MCP1)、氧化型低密度脂蛋白(oxLDL)、巨噬细胞炎症蛋白2(GROβ)、中性粒细胞激活蛋白3(NAP3)测定。应用SPSS 17.0统计学软件对数据进行统计学分析。结果超重腹型肥胖正常高值血压组与对照组人群平均年龄[(50.6±9.1)比(51.3±10.4)岁],性别比例差异无统计学意义。超重腹型肥胖正常高值血压组BMI[(28.0±3.1)比(22.1±1.6)kg/m^2],腹围[(96.4±8.1)比(80.0±5.5)cm],血压[(132.3±7.3)/(77.4±7.5)比(114.3±6.0)/(71.1±6.5)mm Hg]高于对照组。超重腹型肥胖正常高值血压组cfPWV[(8.79±1.54)比(8.26±1.51)m/s]、低密度脂蛋白胆固醇(LDL-C)[(3.01±0.71)比(2.80±0.64)mmol/L]、三酰甘油[(1.86±1.32)比(1.10±0.63)mmol/L]、空腹血糖[(5.27±0.98)比(4.89±0.94)mmol/L]、oxLDL高于对照组,血高密度脂蛋白胆固醇(HDL-C)低于对照组。相关性分析结果显示,所有入选者中cfPWV与BMI、腹围、收缩压、舒张压呈正相关(r=0.186,0.211,0.185,0.163;均P<0.05),未发现cfPWV与血脂、空腹血糖、炎症指标相关。分组相关性分析结果显示,超重腹型肥胖正常高值血压组中cfPWV与年龄、收缩压呈正相关(r=0.301,0.218;均P<0.05),对照组未发现cfPWV与检测指标具有相关性。多元逐步线性回归分析结果显示,在排除其他因素影响后,腹围和年龄与cfPWV具有相关性(B=0.031,0.024;均P<0.05)。结论本研究发现在超重合并腹型肥胖的正常高值血压者中已存在大动脉僵硬度的改变,该变化发生的相关因素包括腹围、BMI和血压,而腹围是该人群大动脉僵硬度变化的影响因素之一。

关键词：颈股动脉脉搏波传导速度超重腹型肥胖正常高值血压炎症因子

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非糖尿病人群空腹血糖与脑梗死的队列研究

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中华高血压杂志 2017年第11期25卷 1044-1049页

作者：王丰王静黄喆陈朔华吴寿岭北京大学第六医院北京大学精神卫生研究所卫生部精神卫生学重点实验室(北京大学)国家精神心理疾病临床医学研究中心(北京大学第六医院) 北京100191 北京大学医学信息学中心开滦总医院心血管内科

目的研究非糖尿病人群中空腹血糖水平对新发脑梗死的影响。方法选择2006-2010年符合纳入排除标准的开滦集团职工为随访对象,分析不同空腹血糖水平下脑梗死事件的发生情况。结果本研究纳入样本84 002例,年龄为30~70(51.1±13.0)岁,男性66... 详细信息

目的研究非糖尿病人群中空腹血糖水平对新发脑梗死的影响。方法选择2006-2010年符合纳入排除标准的开滦集团职工为随访对象,分析不同空腹血糖水平下脑梗死事件的发生情况。结果本研究纳入样本84 002例,年龄为30~70(51.1±13.0)岁,男性66 464例。随访时间为(47.8±3.2)个月。随访期间有887例观察对象发生脑梗死,累积发病率为1.06%。按性别分层后,男性空腹血糖<4.6、4.6~<5.6及≥5.6mmol/L组的累积发病率分别为1.42%、1.02%、1.41%;女性空腹血糖<4.6、4.6~<5.6及≥5.6mmol/L组的累积发病率分别为0.35%、0.53%、0.67%。应用Cox回归模型校正收缩压、舒张压、总胆固醇、吸烟和饮酒等指标后,在男性人群中,与4.6~5.6mmol/L组相比,空腹血糖≥5.6mmol/L组发生脑梗死事件的相对风险为1.29(95%CI 1.08~1.55)。女性人群中未发现此相关性。结论在非糖尿病人群中,空腹血糖水平≥5.6mmol/L增加男性脑梗死的发生风险。

关键词：新发脑梗死糖尿病空腹血糖

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累积甘油三酯暴露与臂踝脉搏波传导速度的关联

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中国动脉硬化杂志 2017年第6期25卷 618-622,634页

作者：杨慧葛明安利杰吴寿岭开滦总医院肿瘤科河北省唐山市063000 唐山市同济医院河北省唐山市063000 开滦总医院心内科河北省唐山市063000

目的探讨累积甘油三酯暴露(cumTG)与臂踝脉搏波传导速度(BaPWV)的关系。方法选择开滦研究人群中卒中队列、老年人群队列和妊娠高血压队列23499例组成观察人群,最终纳入研究队列的为14662例。根据cumTG将研究对象进行四分位分组。采用偏... 详细信息

目的探讨累积甘油三酯暴露(cumTG)与臂踝脉搏波传导速度(BaPWV)的关系。方法选择开滦研究人群中卒中队列、老年人群队列和妊娠高血压队列23499例组成观察人群,最终纳入研究队列的为14662例。根据cumTG将研究对象进行四分位分组。采用偏相关分析cumTG与BaPWV的相关性,多因素线性回归和多因素Logistic回归分析cumTG对BaPWV的影响。结果随着cumTG的增加,平均BaPWV水平和BaPWV≥1400 cm/s的检出率均呈上升趋势。偏相关分析结果表明,cumTG与BaPWV呈正相关(r=0.512,P<0.05);校正了年龄、性别后cumTG与BaPWV仍呈正相关(r=0.322,P<0.05)。多因素线性回归分析显示,cumTG每增加1 mmol/L·year,BaPWV增加4.507 cm/s。Logistic回归分析表明,校正了其他混杂因素后,与cumTG第一分位组相比,cumTG第二分位组、第三分位组、第四分位组均是BaPWV≥1400 cm/s的危险因素,OR值(95%CI)分别为1.667(1.505~1.845)、2.384(2.111~2.691)、3.287(2.887~3.741)。结论 cumTG与BaPWV呈正相关关系。cumTG是脉搏波传导速度增加的危险因素。

关键词：甘油三酯累积甘油三酯暴露臂踝脉搏波传导速度偏相关分析回归分析

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Successful management of severe hypoglycemia induced by total parenteral nutrition in patients with hepatocellular injury: Three cases reports

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World Journal of Clinical Cases 2024年第1期12卷 157-162页

作者： ling-Zhi Fang Hui-Xin Jin Na Zhao Yu-Pei wu Ying-Qin Shi Department of Pharmacy Hebei Key Laboratory of Clinical PharmacyHebei General HospitalShijiazhuang 050051Hebei ProvinceChina

BACKGROUND Glucose imbalance is common in total parenteral nutrition(TPN).Hypoglycemia seems to be less frequent than hyperglycemia,but it influences the clinical outcome to a greater ***,it should be effectively prevented and ***,there is no relevant report on how to treat hypoglycemia caused by TPN in patients with liver cell *** SUMMARY We present three patients with liver cell injury who developed severe hypo-glycemia during or after TPN *** causes of severe hypoglycemia and glucose-raising strategies were *** to the physiological charac-teristics of the hepatocellular injury,the ratio of nutrition components prescribed in TPN was appropriately adjusted for the three *** simultaneously reduced the dose of insulin and fat emulsion,and increased the dose of glucose in *** blood glucose level was restored to normal range and clinical symptoms were *** When hypoglycemia occurs during or after TPN in patients with hepatocellular injury,physicians need to simultaneously reduce insulin and fat emulsion,and increase glucose,and correct severe hypoglycemia in time to reduce its adverse consequences.

关键词： Total parenteral nutrition Hepatocellular injury Severe hypoglycemia Treatment Causes Case report

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Retinal nerve fiber layer defects and chronic kidney disease:the Kailuan Eye Study

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International Journal of Ophthalmology(English edition) 2024年第9期17卷 1696-1706页

作者： Qian-Qian Wan Jin-Qiong Zhou Li-Jian Fang Ya-Xing Wang Ye-Nan Wang Qian Wang Yan-Ni Yan Xuan Yang shou-ling wu Shuo-Hua Chen Jost B Jonas Wen-Bin Wei Beijing Tongren Eye Center Beijing Key Laboratory of Intraocular Tumor Diagnosis and TreatmentBeijing Ophthalmology&Visual Sciences Key LabMedical Artificial Intelligence Research and Verification Key Laboratory of the Ministry of Industry and Information TechnologyBeijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Ophthalmology the Second Hospital of Anhui Medical UniversityHefei 230000Anhui ProvinceChina Department of Ophthalmology Beijing Liangxiang HospitalCapital Medical UniversityBeijing 102400China Beijing Institute of Ophthalmology Beijing Key Laboratory of Ophthalmology and Visual SciencesBeijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Ophthalmology Xuanwu HospitalCapital Medical UniversityBeijing 100730China Department of Cardiology Kailuan General HospitalTangshan 063000Hebei ProvinceChina Health Care Center Kailuan GroupTangshan 063000Hebei ProvinceChina Department of Ophthalmology Medical Faculty Mannheim of the Ruprecht-Karls-University of HeidelbergHeidelberg 69117Germany

AIM:To investigate whether retinal nerve fiber layer defects(RNFLDs)is a potential risk factor for chronic kidney disease(CKD)in Chinese ***:The Kailuan Eye Study was a populationbased study that included 14440 *** participants underwent detailed assessments,RNFLDs were diagnosed using color fundus ***:Overall,12507 participants[8533 males(68.23%)]had complete systemic examination data and at least one evaluable fundus *** were found in 621 participants[5.0%;95%confidence interval(CI):4.6%-5.34%],and 70 cases of multiple RNFLDs were found(11.27%).After adjusting multiple factors,RNFLDs was significantly associated with CKD severity,the ORs of CKD stage 3,stage 4 and stage 5 were 1.698,4.167,and 9.512,*** RNFLDs were also associated with CKD severity after adjusting multiple factors,the ORs of CKD stage 3 and stage 5 were 4.465 and 11.833 ***,2294 participants had CKD(18.34%,95%CI:17.68%-18.99%).After adjusting for other factors,CKD presence was significantly correlated with the presence of ***:The strongest risk factors for RNFLDs are CKD and ***,RNFLDs can be an ocular feature in patients with *** can help detect systemic diseases,and assessment for RNFLDs should be considered in CKD patients.

关键词： chronic kidney disease retinal nerve fiber layer defects Kailuan Eye Study fundus examination

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Synthesis of Long-Chain Oligomeric Donor and Acceptors via Direct Arylation for Organic Solar Cells

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Chinese Journal of Chemistry 2024年第5期42卷 523-532页

作者： Yu wu Xin-Yu He Xu-Min Huang ling-Jun Yang Peng Liu Na Chen Chang-Zhi Li Shi-Yong Liu Jiangxi Provincial Key Laboratory of Functional Molecular Materials Chemistry College of MaterialsMetallurgical and ChemistryJiangxi University of Science and TechnologyGanzhouJiangxi341000 China Department of Polymer Science and Engineering Zhejiang UniversityHangzhouZhejiang310027 China

The rapid synthesis of structurally complicated electron donors&acceptors still remains a major challenge in organic solar cells(OSC).In this work,we developed a highly efficient strategy to access long-chain oligomeric donor and acceptors for OSC applications.A series of cyclopentadithiophene(CPDT)and benzothiadiazole(BT)-basedπ-conjugated oligomers,i.e.,three oligomeric acceptors(BTDT)n-IC(n=1—3)and one long-chain oligomeric donor(BTDT)4-RD,are facilely synthesized by an atom-and step-economical,and labor-saving direct C—H arylation(DACH)reaction(i.e.,C—H/C—Br cross coupling).Note that(BTDT)4-RD involving five CPDT,four BT and two rhodamine(RD)building blocks is the longest oligomeric donor in the fullerene-free OSC devices ever *** dependence of the structure-property-performance correlation of(BTDT)n-IC(n=1—3)and(BTDT)4-RD on theπ-conjugation lengths is thoroughly investigated by opto-electrochemical measurements,bulk heterojunction(BHJ)OSC devices and ***(BTDT)1-IC:PBDB-T and(BTDT)4-RD:Y6 BHJs achieve power conversion efficiencies of 9.14%and 4.51%,*** findings demonstrate that DACH reaction is a powerful tool to tune the opto-electronic properties and device performances by regulating the lengths ofπ-conjugated oligomers with varied numbers of repeating units.

关键词： Long-chainπ-conjugated oligomer Organic solar cells Direct arylation Atom-economical synthesis Cyclopentadithiophene Benzothiadiazole C—H activation Conjugation Oligomers

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Multi-performance evaluation of high-throughput accelerated corrosion test for high-strength low-alloy 921A steel

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Journal of Iron and Steel Research International 2024年第5期31卷 1260-1278页

作者： Cong-cong Du Ming-hua Qin Zhan-fang wu Dong-ling Li Lei Zhao Xiang-yang Li Hai-zhou Wang Beijing Advanced Innovation Center for Materials Genome Engineering National Center for Materials Service SafetyUniversity of Science and Technology BeijingBeijing100083China China Iron and Steel Research Institute Group Co. Ltd.Beijing100081China Beijing Advanced Innovation Center for Materials Genome Engineering Central Iron and Steel Research InstituteBeijing100081China

The corrosion behavior of high-strength low-alloy 921A steel in a simulated marine atmospheric environment was studied using a high-throughput experimental *** corrosion behavior,corrosion morphology,and corrosion products of 921A steels were analyzed using various techniques,including corrosion mass loss method,polarization curve,white-light interferometry,scanning electron microscopy,energy-dispersive spectrometry,microbeam X-ray fluorescence spectrometry,X-ray diffraction technique,and X-ray photoelectron *** test results indicated that 921A steel exhibits better corrosion resistance than Q450NQR1 steel in simulated harsh atmospheric environments,as evidenced by a lower corrosion mass loss rate throughout the corrosion *** corrosion products of both steels consisted ofα-FeOOH,Fe_(3)O_(4),andγ-FeOOH,withα-FeOOH being more prevalent in the rust layer of 921A steel than in Q450NQR1 *** inner rust layer of 921A steel also exhibited an appositional enrichment region of Cr,Ni,Mo,and V,leading to its superior corrosion resistance compared to that of Q450NQR1 *** efficacy of high-throughput accelerated corrosion experimental methods was highlighted for evaluating the corrosion resistance of steel materials in harsh environmental *** findings suggest that 921A steel exhibits better corrosion resistance compared to Q450NQR1 steel and has the potential to be more suitable in harsh marine atmospheric *** characterization of the rust layer structures and composition reveals the parallel enrichment of certain elements in the inner rust layer of 921A steel,which enhances its corrosion resistance.

关键词： Atmospheric corrosion High-throughput experimental method High-strength low-alloy steel Corrosion characteristics Rust layer Element enrichment

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甘油三酯与臂踝动脉脉搏波传导速度的关系

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中国循环杂志 2017年第5期32卷 470-474页

作者：杨慧吴寿岭李晓芳姜宇武爱萍刘颖开滦总医院肿瘤科河北省唐山市036000 开滦总医院心内科河北省唐山市036000 开滦总医院神经内科河北省唐山市036000

目的:探讨甘油三酯与臂踝动脉脉搏波传导速度(baPWV)的关系。方法:选择开滦研究人群(卒中队列5 219例、老年人群队列1 754例、妊娠高血压队列1 561例、外周血管疾病筛查人群14 088例)共22 622例完成baPWV检测,采用偏相关分析甘油三酯与b... 详细信息

目的:探讨甘油三酯与臂踝动脉脉搏波传导速度(baPWV)的关系。方法:选择开滦研究人群(卒中队列5 219例、老年人群队列1 754例、妊娠高血压队列1 561例、外周血管疾病筛查人群14 088例)共22 622例完成baPWV检测,采用偏相关分析甘油三酯与baPWV的相关性,多因素线性回归、多因素Logistic回归分析甘油三酯对baPWV的影响。结果:在22 622例人群中,排除甘油三酯缺失909例,最终纳入研究队列的为21 713例。结果显示,随着甘油三酯水平的增加,平均baPWV水平和baPWV≥1400 cm/s的检出率均呈上升趋势,偏相关分析结果表明甘油三酯与baPWV之间呈正相关(r=0.235),校正年龄、性别后甘油三酯与baPWV仍呈正相关(r=0.080)。Logistics回归分析表明,校正其他混杂因素后甘油三酯第二分位组、第三分位组、第四分位组与甘油三酯第一分位组相比均是baPWV≥1 400 cm/s的危险因素,比值比及95%可信区间分别为1.161(1.035~1.302)、1.312(1.165~1.476)、1.652(1.463~1.865)。结论:甘油三酯与脉搏波传导速度呈正相关,甘油三酯与脉搏波传导速度独立关联。

关键词：甘油三酯脉搏波传导速度动脉僵硬度

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Revealing the Structure-Property Difference of Natural Rubber Prepared by Different Methods: Protein and Gel Content are Key Factors

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Chinese Journal of Polymer Science 2024年第4期42卷 457-467页

作者： Shao-Qi Huang Jun-Qi Zhang Yong Zhu ling-Min Kong Lu-Sheng Liao Fu-Quan Zhang Zheng-Tian Xie Jin-Rong wu State Key Laboratory of Polymer Materials Engineering College of Polymer Science and EngineeringSichuan UniversityChengdu610065China Hainan Provincial Key Laboratory of Natural Rubber Processing Agricultural Products Processing Research Institute of Chinese Academy of Tropical Agricultural SciencesZhanjiang524001China

Natural rubber (NR) is widely used in various fields including aerospace, military industry and transportation due to its superior elasticity and comprehensive mechanical properties. Nonetheless, the commercial NR prepared by different methods usually exhibits different mechanical properties, primarily due to variations in processing conditions during the conversion from latex to bulk rubber material. Consequently, this poses challenges in scientific research and industrial production of NR. In order to assess the properties of various commercially available NR and identify key structural and compositional components, this study systematically compares and analyzes four representative NR raw materials: air dried sheet (ADS), ribbed smoked sheets (RSS), constant viscidity NR (CV), and whole field latex rubber (WF). The investigation focuses on evaluating their static mechanical behavior, SIC behavior, wear resistance, and fatigue resistance. The findings indicate that protein and gel content exhibit a crucial influence on the NR properties. These constituents contribute to the formation of a high-crosslinking density region, generating a heterogeneous network structure within the rubber. This structure amplifies strains during deformation, leading to earlier and stronger strain-induced crystallization (SIC). Among the four commercial NR brands, RSS demonstrates superior overall mechanical and dynamic properties owing to its high protein and gel content. This study serves as a valuable reference for comprehending the differences in properties among various commercial NR, thereby offering guidance for the actual processing and selection of NR.

关键词： Natural rubber Processingmethod Protein Gel content Property

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