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MAPK signaling： A key element in plant defense response to insects

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Insect Science 2015年第2期22卷 157-164页

作者： Christian Hettenhausen Meredith C. Schuman jianqiang wu Kunming Institute of Botany Chinese Academy of Sciences Kunming China Max Planck Institute for Chemical Ecology Hans-Knoell Str. 8 07745 Jena Germany

Insects have long been the most abundant herbivores, and plants have evolved sophisticated mechanisms to defend against their attack, In particular, plants can perceive specific patterns of tissue damage associated with insect herbivory. Some plant species can perceive certain elicitors in insect oral secretions （OS） that enter wounds during feeding, and rapidly activate a series of intertwined signaling pathways to orchestrate the biosynthesis of various defensive metabolites. Mitogen-activated protein kinases （MAPKs）, common to all eukaryotes, are involved in the orchestration of many cellular processes, including development and stress responses. In plants, at least two MAPKs, salicylic acid-induced protein kinase （SIPK） and wound-induced protein kinase （WlPK）, are rapidly activated by wounding or insect 0S; importantly, genetic studies us- ing transgenic or mutant plants impaired in MAPK signaling indicated that MAPKs play critical roles in regulating the herbivory-induced dynamics of phytohormones, such as jasmonic acid, ethylene and salicylic acid, and MAPKs are also required for transcriptional activation of herbivore defense-related genes and accumulation of defensive metabolites. In this review, we summarize recent developments in understanding the functions of MAPKs in plant resistance to insect herbivores.

关键词： defense FAC insect jasmonate mitogen-activated protein kinase (MAPK) plant-insect interaction

Arabidopsis Plants Having Defects in Nonsense-mediated mRNA Decay Factors UPF1, UPF2, and UPF3 Show Photoperiod-dependent Phenotypes in Development and Stress Responses

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Journal of Integrative Plant Biology 2012年第2期54卷 99-114页

作者： Chuan Shi Ian T. Baldwin jianqiang wu Department of Medical Engineering and Biotechnology University of Applied Sciences Jena 07745 Germany Department of Molecular Ecology Max Planck Institute for Chemical Ecology 07745 Germany

Nonsense-mediated mRNA decay （NMD） is an important mRNA quality surveillance pathway in all eukaryotes that eliminates aberrant mRNAs derived from various sources. Three NMD factor proteins, UPF1, UPF2, and UPF3 are required for the NMD process and were found to be also involved in certain stress responses in mammalian and yeast cells. Using Arabidopsis thaliana mutants of UPF1 and UPF3 and UPF2-silenced lines （irUPF2）, we examined the involvement of UPF1, UPF2, and UPF3 in development and in response to stresses, wounding and infection by Pseudomonas syringae pv. tomato strain DC3000. Under the long （16 h） photoperiod condition, Arabidopsis having a defect in NMD factors exhibited altered morphologies of various organs, disturbed homeostasis of wounding-induced jasmonic acid and pathogen-elicited salicylic acid, and abnormal wounding- and methyl jasmonate-induced changes in the transcript levels of two defense-related genes, LOX2 and VSP2. Importantly, when plants were cultivated under the short （10 h） photoperiod condition, mutants of UPF1 and UPF3 and irUPF2 showed smaller differences from the wild-type plants in growth and stress-induced responses. These data suggest a complex regulatory network, likely composed of light signaling and NMD factor-mediated pathways, in influencing plant development and adaption to environmental stresses.

关键词： Development nonsense-mediated mRNA decay pathogen photoperiod wounding

Silencing Brassinosteroid Receptor BRI1 Impairs Herbivory-elicited Accumulation of Jasmonic Acid-isoleucine and Diterpene Glycosides, but not Jasmonic Acid and Trypsin Proteinase Inhibitors in Nicotiana attenuata

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Journal of Integrative Plant Biology 2013年第6期55卷 514-526页

作者： Da-Hai Yang Ian T. Baldwin jianqiang wu Department of Molecular Ecology Max Planck Institute for Chemical Ecology Hans-Knll Stra e 8 D-07745 Jena Germany Plant Stress Biology Group Shanghai Center for Plant Stress Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Key Laboratory of Economic Plants and Biotechnology Kunming Institute of Botany the Chinese Academy of Sciences

The brassinosteroid （BR） receptor, BR insensitive 1 （BRI1）, plays a critical role in plant development, but whether BRI1-mediated BR signaling is involved in plant defense responses to herbivores was largely unknown. Here, we examined the function of BRI1 in the resistance of Nicotiana attenuata （Solanaceae） to its specialist insect herbivore Manduca sexta. Jasmonic acid （JA） and JA-isoleucine conjugate （JA-Ile） are important hormones that mediate resistance to herbivores and we found that after wounding or simulated herbivory NaBRI1 had little effect on JA levels, but was important for the induction of JA-Ile. Further experiments revealed that decreased JAR （the enzyme for JA-Ile production） activity and availability of Ile in NaBRI1-silenced plants were likely responsible for the low JA-Ile levels. Consistently, M. sexta larvae gained more weight on NaBRI1-silenced plants than on the control plants. Quantification of insect feeding-induced secondary metabolites revealed that silencing NaBRI1 resulted in decreased levels of carbon-rich defensive secondary metabolites （hydroxygeranyllinalool diterpene glycosides, chlorogenic acid, and rutin）, but had little effect on the nitrogen-rich ones （nicotine and trypsin proteinase inhibitors）. Thus, NaBRI1-mediated BR signaling is likely involved in plant defense responses to M. sexta, including maintaining JA-Ile levels and the accumulation of several carbon-rich defensive secondary metabolites.

关键词： BRI1 herbivore insect jasmonic acid Nicotiana attenuata secondary metabolites.

Inter-species mRNA transfer among green peach aphids,dodder parasites,and cucumber host plants

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Plant Diversity 2022年第1期44卷 1-10页

作者： Juan Song Jinge Bian Na Xue Yuxing Xu jianqiang wu Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China

mRNAs are transported within a plant through *** are phloem feeders and dodders(Cuscuta spp.)are parasites which establish phloem connections with host *** aphids feed on dodders,whether there is trafficking of mRNAs among aphids,dodders,and host plants and if aphid feeding a匚fects the mRNA transfer between dodders and hosts are *** constructed a green peach aphid(GPA,Myzus persicae)-dodder(Cuscuta austra/is)-cucumber(Cucumis sativus)tritrophic system by infesting GPAs on ***,which parasitized cucumber *** found that GPA feeding activated defense-related phytohormonal and transcriptomic responses in both *** and cucumbers and large numbers of mRNAs were found to be transferred between *** and cucumbers and between *** and GPAs;importantly,GPA feeding on *** greatly altered inter-species mobile mRNA ***,three cucumber mRNAs and three GPA mRNAs could be respectively detected in GPAs and ***,our statistical analysis indicated that mRNAs with high abundances and long transcript lengths are likely to be *** study reveals the existence of inter-species and even inter-kingdom mRNA movement among insects,parasitic plants,and parasite hosts,and suggests complex regulation of mRNA trafficking.

关键词： Dodder Cuscuta Green peach aphid Host plant mRNA transfer Intei^kingdom interaction

MYC2, MYC3, and MYC4 function additively in wounding-induced jasmonic acid biosynthesis and catabolism

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Journal of Integrative Plant Biology 2020年第8期62卷 1159-1175页

作者： Cuiping Zhang Yunting Lei Chengkai Lu Lei Wang jianqiang wu Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Jasmonic acid(JA)plays a critical role in plant defenses against insects and necrotrophic *** or lepidopteran insect feeding rapidly induces a burst of JA in plants,which usually reaches peak values within 1 to 2 *** induced JA is converted to JA-Ile and perceived by the COI1-JAZ co-receptor,leading to activation of the transcription factors MYC2 and its homologs,which further induce JA-responsive *** much is known about JA biosynthesis and catabolism enzymes and JA signaling,how JA biosynthesis and catabolism are regulated remain ***,we show that in Arabidopsis thaliana MYC2 functions additively with MYC3 and MYC4 to regulate wounding-induced JA accumulation by directly binding to the promoters of genes function in JA biosynthesis and catabolism to promote their ***2 also controls the transcription of JAV1 and JAM1,which are key factors controlling JA biosynthesis and catabolism,*** addition,we also found that MYC2 could bind to the MYC2 promoter and self-inhibit its own *** work illustrates the central role of MYC2/3/4 in controlling wounding-induced JA accumulation by regulating the transcription of genes involved in JA biosynthesis and catabolism.

关键词： expression. MYC2 catabolism

Silencing JA hydroxylases in Nicotiana attenuata enhances jasmonic acid-isoleucine-mediated defenses against Spodoptera litura

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Plant Diversity 2020年第2期42卷 111-119页

作者： Jinxiang Tang Dahai Yang jianqiang wu Suiyun Chen Lei Wang Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming650201China Tobacco Breeding and Biotechnology Research Center Yunnan Academy of Tobacco Agricultural SciencesKey Laboratory of Tobacco Biotechnological BreedingNational Tobacco Genetic Engineering Research CenterKunming650021China School of Life Science Yunnan UniversityKunming650091China

Jasmonic acid(JA)plays important roles in plant resistance to insect *** important derivative of JA is 12-OH-JA,which is produced by two independent pathways:direct hydroxylation of JA by jasmonate-induced oxygenases(JOXs)or hydrolyzation of *** the function of 12-OH-JA in plant-herbivore interactions remains largely *** this study,we silenced four JOX homologs independently in the wild tobacco Nicotiana attenuata by virus-induced gene silencing(VIGS),and found that all four JOX homologs are involved in JA *** silencing the four JA hydroxylases in VIGS-NaJOXs plants decreased herbivory-induced 12-OH-JA by 33%,but JA and JA-Ile levels increased by 45%and 30%,respectively,compared to those in control *** to direct hydroxylation from JA,hydrolyzation from 12-OH-JA-Ile is equally important for herbivory-induced 12-OHJA accumulation:in the 12-OH-JA-Ile deficient irJAR4/6 plants,12-OH-JA decreased 34%.Moreover,VIGSNaJOXs plants exhibited enhanced resistance to the generalist herbivore Spodoptera *** poor larval performance was strongly correlated with high levels of several JA-Ile-dependent direct defense metabolites in the VIGS-NaJOXs *** we simultaneously silenced all four JA hydroxylases in the JAIle-deficient irJAR4/6 background,the enhanced herbivore resistance diminished,demonstrating that enhanced herbivore resistance resulted from elevated JA-Ile *** that silencing these NaJOX-like genes did not detectably alter plant growth but highly increased plant defense levels,we propose that JOX genes are potential targets for genetic improvement of herbivore-resistant crops.

关键词： JA metabolism 12-OH-JA Spodoptera litura 2-Oxoglutarate oxygenase Herbivore defense Nicotiana attenuata

Bacterial community response to petroleum contamination in brackish tidal marsh sediments in the Yangtze River Estuary, China

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Journal of Environmental Sciences 2021年第1期33卷 160-167页

作者： Min Wang Chenyan Sha Jian wu Jinghua Su jianqiang wu Qing Wang Juan Tan Shenfa Huang Research Institute of Natural Ecology Conseruation Shanghai Academy of Enuvironmental Sciences508 Qinzhou RoadShanghai 200233China

The brackish tidal marsh in the Baimaosha area of the Yangtze River Estuary was severely contaminated by 400 tons of heavy crude petroleum from a tanker that sank in December *** spill accident led to severe environmental damage owing to its high toxicity,persistence and wide *** communities play vital roles in petroleum degradation in marsh ***,taxonomic analysis,high-throughput sequencing and 16 S rRNA functional prediction were used to analyze the structure and function of microbial communities among uncontaminated(CK),lightly polluted(LP),heavily polluted(HP),and treated(TD)*** bacterial communities responded with increased richness and decreased diversity when exposed to petroleum *** dominant class changed from Deltaproteobacteria to Gammaproteobacteria after petroleum *** phylum Firmicutes increased dramatically in oil-enriched sediment by 75.78%,346.19%and 267.26%in LP,HP and TD,*** of the suspected oil-degrading genera,Dechloromonas,increased the most in oil-contaminated sediment,by 540.54%,711.27%and 656.78%in LP,HP and TD,*** protease,quinate dehydrogenase(quinone)and glutathione-independent formaldehyde dehydrogenase,three types of identified enzymes,increased enormously with the increasing petroleum *** conclusion,petroleum contamination altered the community composition and microorganism structure,and promoted some bacteria to produce the corresponding degrading ***,the suspected petroleum-degrading genera should be considered when restoring oil-contaminated sediment.

关键词： Brackish marsh sediment Petroleum contamination Bacterial community Gene enzyme

The Essential Role of Jasmonic Acid in Plant-Herbivore Interactions-Using the Wild Tobacco Nicotiana attenuata as a Model

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Journal of Genetics and Genomics 2013年第12期40卷 597-606页

作者： Lei Wang jianqiang wu Key Laboratory of Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of Sciences

The plant hormone jasmonic acid （JA） plays a central role in plant defense against herbivores. Herbivore damage elicits a rapid and transient JA burst in the wounded leaves and JA functions as a signal to mediate the accumulation of various secondary metabolites that confer resistance to herbivores. Nicotiana attenuata is a wild tobacco species that inhabits western North America. More than fifteen years of study and its unique interaction with the specialist herbivore insect Manduca sexta have made this plant one of the best models for studying plant-herbivore interactions. Here we review the recent progress in understanding the elicitation of JA accumulation by herbivore-specific elicitors, the regulation of JA biosynthesis, JA signaling, and the herbivore-defense traits in N. attenuata.

关键词： Jasmonic acid Defense Plant--herbivore interaction Nicotiana attenuata Manduca sexta

The key cyclic electron flow protein PGR5 associates with cytochrome b_(6)f, and its function is partially influenced by the LHCⅡ state transition

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Horticulture Research 2021年第1期8卷 754-765页

作者： Xinyi wu jianqiang wu Yu Wang Meiwen He Mingming He Weikang Liu Sheng Shu Jin Sun Shirong Guo College of Horticulture Nanjing Agricultural UniversityNanjing 210095China Nanjing Agricultural University(Suqian)Academy of Protected Horticulture JiangsuSuqian 223800China

In plants and algae,PGR5-dependent cyclic electron flow(CEF)is an important regulator of acclimation to fluctuating environments,but how PGR5 participates in CEF is *** this work,we analyzed two PGR5s in cucumber(Cucumis sativus L.)under different conditions and found that CsPGR5a played the dominant role in PGR5-dependent *** results of yeast two-hybrid,biomolecular fluorescence complementation(BiFC),blue native PAGE,and coimmunoprecipitation(CoIP)assays showed that PGR5a interacted with PetC,Lhcb3,and ***,the intensity of the interactions was dynamic during state transitions,and the abundance of PGR5 attached to cyt b_(6)f decreased during the transition from state 1 to state 2,which revealed that the function of PGR5a is related to the state *** proposed that PGR5 is a small mobile protein that functions when attached to protein complexes.

关键词： transition. state attached