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Determination of double-and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity

Journal of Animal Science and Biotechnology 2023年第1期14卷 134-151页

作者： Jordi Ribas‑Maynou Ariadna Delgado‑Bermúdez Yentel Mateo‑Otero Estel Vinolas Carlos O.Hidalgo wsteven ward Marc Yeste Biotechnology of Animal and Human Reproduction(TechnoSperm) Faculty of SciencesInstitute of Food and Agricultural TechnologyUniversity of GironaC/Maria Aurèlia Campany69ES‑17003 GironaSpain. Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaES‑17003 GironaSpain. Institute for Biogenesis Research Department of AnatomyBiochemistry&PhysiologyJohn A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96822USA. Department of Animal Selection and Reproduction The Regional Agri-Food Research and Development Service of Asturias(SERIDA)ES‑33394 GijónSpain. Catalan Institution for Research and Advanced Studies(ICREA) ES‑08010 BarcelonaSpain.

Background:The analysis of chromatin integrity has become an important determinant of sperm *** frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well *** aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in ***:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and *** of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage

关键词： Cattle Chromatin Comet test DNA damage Fertility Sperm Sperm quality

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