检索结果-南通市图书馆

Laboratory characteristics of recent hepatitis A in Korea:Ongoing epidemiological shift

World Journal of Gastroenterology 2010年第9期16卷 1115-1118页

作者： Hee won Moon Jin Hee Cho Mina Hur Yeo-Min Yun won hyeok choe So Young Kwon Chang Hong Lee Department of Laboratory Medicine Konkuk University School of MedicineKonkuk University Medical Center Department of Internal Medicine Konkuk University School of MedicineKonkuk University Medical Center

AIM:To evaluate seroprevalence of hepatitis A virus (HAV) antibody and investigate demographic,clinical,and laboratory features of recent cases in ***:For the evaluation of hepatitis A seroprevalence,we analyzed the data from 3127 subjects including,healthcare workers and patients who visited Konkuk University Hospital,a secondary referral center,from January to October *** sera with positive IgM were excluded from seroprevalence data for total HAV *** retrospectively reviewed the electronic medical records of 419 patients with HAV,who were diagnosed by the presence of serum IgM antibodies against *** patients presented at Konkuk University Hospital between August 2005 and September ***:Among 3127 sera tested,1428 (45.7%)were positive for anti-HAV *** seroprevalence was very low in teenagers or those in their twenties,increased in those in their thirties,and was > 90% in older *** children younger than 10 years,seroprevalence was increased *** patients with HAV hepatitis were in their twenties and *** γ-glutamyl transpeptidase increased with age and was significantly higher in patients older than 30 *** of severity,such as decreased albumin and increased bilirubin,were also more prominent in the older age group;however,the leukocyte count was higher and the frequency of leukopenia was lower in younger patients than in older ***:There has been an apparent epidemiological shift in HAV seroprevalence and a change in the peak age of HAV *** study could provide baseline data of recent hepatitis A in Asia.

关键词： Hepatitis A virus Seroprevalence Epidemiology Korea

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections,rt269L and rt269I

引用

World Journal of Gastroenterology 2023年第11期29卷 1721-1734页

作者： Kijeong Kim Yu-Min Choi Dong Hyun Kim Junghwa Jang won hyeok choe Bum-Joon Kim Department of Microbiology College of MedicineChung-Ang UniversitySeoul 06974South Korea Department of Microbiology and Immunology College of MedicineSeoul National UniversitySeoul 03080South Korea Department of Internal Medicine Konkuk University School of MedicineSeoul 05030South Korea Department of Biomedical Sciences College of MedicineSeoul National UniversitySeoul 03080South Korea Liver Research Institute College of MedicineSeoul National UniversitySeoul 03080South Korea Seoul National University Medical Research Center Seoul 03080South Korea

BACKGROUND The presence of two distinct hepatitis B virus(HBV)Pol RT polymorphisms,rt269L and rt269I,could contribute to the unique clinical or virological phenotype of HBV genotype ***,a simple and sensitive method capable of identifying both types in chronic hepatitis B(CHB)patients infected with genotype C2 should be *** To develop a novel simple and sensitive locked nucleic acid(LNA)-real timepolymerase chain reaction(RT-PCR)method capable of identifying two rt269 types in CHB genotype C2 *** We designed proper primer and probe sets for LNA-RT-PCR for the separation of rt269 *** synthesized DNAs of the wild type and variant forms,melting temperature analysis,detection sensitivity,and endpoint genotyping for LNA-RT-PCR were *** developed LNA-RT-PCR method was applied to a total of 94 CHB patients of genotype C2 for the identification of two rt269 polymorphisms,and these results were compared with those obtained by a direct sequencing *** The LNA-RT-PCR method could identify two rt269L and rt269I polymorphisms of three genotypes,two rt269L types[‘L1’(WT)and‘L2’]and one rt269I type(‘I’)in single(63 samples,72.4%)or mixed forms(24 samples,27.6%)in 87(92.6%sensitivity)of 94 samples from Korean CHB *** the results were compared with those obtained by the direct sequencing protocol,the LNA-RT-PCR method showed the same results in all but one of 87 positive detected samples(98.9%specificity).CONCLUSION The newly developed LNA-RT-PCR method could identify two rt269 polymorphisms,rt269L and rt269I,in CHB patients with genotype C2 *** method could be effectively used for the understanding of disease progression in genotype C2 endemic areas.

关键词： Hepatitis B virus Genotype C2 Polymerase rt269 Locked nucleic acid-real time-polymerase chain reaction Chronic hepatitis B

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Naturally occurring mutations in the reverse transcriptase region of hepatitis B virus polymerase from treatment-na?ve Korean patients infected with genotype C2

引用

World Journal of Gastroenterology 2017年第23期23卷 4222-4232页

作者： Ji-Eun Kim So-Young Lee Hong Kim Ki-Jeong Kim won-hyeok choe Bum-Joon Kim Department of Biomedical Sciences Microbiology and Immunologyand Liver Research InstituteSeoul National University College of Medicine Department of Microbiology School of MedicineJoong-Ang University Department of Internal Medicine Konkuk University School of Medicine

AIM To report naturally occurring mutations in the reverse transcriptase region(RT) of hepatitis B virus(HBV) polymerase from treatment na?ve Korean chronic patients infected with genotype *** Here, full-length HBV reverse transcriptase RT sequences were amplified and sequenced from 131 treatment na?ve Korean patients chronically infected with hepatitis B genotype C2. The patients had two distinct clinical statuses: 59 patients with chronic hepatitis(CH) and 72 patients with hepatocellular carcinoma(HCC). The deduced amino acids(AAs) at42 previously reported potential nucleos(t)ide analog resistance(NAr) mutation positions in the RT region were analyzed. RESULTS Potential NAr mutations involving 24 positions were found in 79 of the 131 patients(60.3%). Notably, AA substitutions at 2 positions(rt184 and rt204) involved in primary drug resistance and at 2 positions(rt80 and rt180) that functioned as secondary/compensatory mutations were detected in 10 patients(1 CH patient and 9 HCC patients) and 7 patients(1 CH and 6 HCC patients), respectively. The overall mutation frequencies in the HCC patients(3.17%, 96/3024 mutations) were significantly higher than the frequencies in the CH patients(2.09%, 52/2478 mutations)(P = 0.003). In addition, a total of 3 NAr positions, rt80, rt139 and rt204 were found to be significantly related to HCC from treatment na?ve Korean patients. CONCLUSION Our data showed that naturally occurring NAr mutations in South Korea might contribute to liver disease progression(particularly HCC generation) in chronic patients with genotype C2 infections.

关键词： Hepatitis B virus Polymerase Reverse transcriptase Potential nucleos(t)ide analog resistance Chronic hepatitis Hepatocellular carcinoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparative study for predictability of type 1 gastric variceal rebleeding after endoscopic variceal ligation:High-frequency intraluminal ultrasound study

引用

World Journal of Clinical Cases 2021年第34期9卷 10566-10575页

作者： Jeong Hwan Kim won hyeok choe Sun-Young Lee So Young Kwon In-Kyung Sung Hyung Seok Park Department of Internal Medicine Konkuk University School of MedicineKonkuk University Medical CenterSeoul 05030South Korea Department of Internal Medicine Konkuk University Medical CenterSeoul 143729South Korea Department of Internal Medicine Konkuk University School of MedicineSeoul 05030South Korea Department of Internal Medicine Digestive Disease CenterKonkuk University School of MedicineSeoul 05030South Korea

BACKGROUND The efficacy of endoscopic ultrasonography for the follow-up of gastric varices treated with endoscopic variceal ligation(EVL)has not been *** To evaluate the diagnostic correlation of esophagogastroduodenoscopy(EGD)and high-frequency intraluminal ultrasound(HFIUS)for type 1 gastric varices(GOV1)after EVL and to identify the predictability for rebleeding of EGD and *** In liver cirrhosis patients with GOV1,we performed endoscopic follow-up using EGD and HFIUS synchronously after EVL for hemorrhage from *** grading and red color signs were analyzed using EGD,and the largest variceal cross-sectional areas were measured using *** addition,1-year follow-up was *** rebleeding was defined as the presence of hematemesis,hematochezia,or melena without other evidence of bleeding on endoscopic *** In 26 patients with GOV1,variceal cross-sectional areas on HFIUS of GOV1 was poorly correlated with EGD grading of GOV1(r=0.36).In 17 patients who completed the 1-year follow-up,variceal cross-sectional areas on HFIUS was a good predictor of subsequent rebleeding,whereas EGD grading was not a predictor of subsequent *** HFIUS measurement is more predictive of GOV1 rebleeding than EGD grading,so HFIUS measurement may be necessary for endoscopic follow-up after EVL in patients with GOV1.

关键词： Endoscopic variceal ligation Esophagogastroduodenoscopy High-frequency intraluminal ultrasound Rebleeding Type 1 gastric varices

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Short-term interval combined chemoembolization and radiofrequency ablation for hepatocellular carcinoma

引用

World Journal of Gastroenterology 2014年第35期20卷 12588-12594页

作者： won hyeok choe Young Jun Kim Hee Sun Park Sang Woo Park Jeong Han Kim So Young Kwon Department of Internal Medicine Konkuk University School of Medicine Department of Radiology Konkuk University School of Medicine

AIM: To investigate hepatic function after combined transcatheter arterial chemoembolization (TACE) and radiofrequency ablation (RFA) with a short-term interval (0-2 d).

关键词： Child-Pugh score Liver cirrhosis Hepatocellular carcinoma Transcatheter arterial chemoembolization Radiofrequency ablation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Three-Tesla magnetic resonance elastography for hepatic fibrosis:Comparison with diffusion-weighted imaging and gadoxetic acid-enhanced magnetic resonance imaging

引用

World Journal of Gastroenterology 2014年第46期20卷 17558-17567页

作者： Hee Sun Park Young Jun Kim Mi Hye Yu won hyeok choe Sung Il Jung Hae Jeong Jeon Department of Radiology Konkuk University School of MedicineSeoul 143-729South Korea Digestive Disease Center Konkuk University School of MedicineSeoul 143-729South Korea

AIM: To evaluate the feasibility of 3-Tesla magnetic resonance elastography (MRE) for hepatic fibrosis and to compare that with diffusion-weighted imaging (DWI) and gadoxetic acid-enhanced magnetic resonance (MR) imag... 详细信息

关键词： Magnetic resonance elastography Hepatic fibrosis Diffusion weighted imaging Gadoxetic acid enhanced magnetic resonance imaging Serum markers

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tenofovir is a more suitable treatment than entecavir for chronic hepatitis B patients carrying naturally occurring rtM204I mutations

引用

World Journal of Gastroenterology 2019年第33期25卷 4985-4998页

作者： won hyeok choe Kijeong Kim So-Young Lee Yu-Min Choi So Young Kwon Jeong Han Kim Bum-Joon Kim Department of Internal Medicine Konkuk University School of MedicineSeoul 05030South Korea Department of Microbiology College of MedicineChung-Ang UniversitySeoul 06974South Korea Department of Biomedical Sciences Microbiology and ImmunologyLiver Research InstituteCancer Research Institute and SNUMRCCollege of MedicineSeoul National UniversitySeoul 03080South Korea

BACKGROUND Hepatitis B virus(HBV) DNA polymerase mutations usually occur to long term use of nucleos(t)ide analogues(NAs), but they can occur spontaneously in treatment-na?ve chronic hepatitis B(CHB) patients. The naturally occurring HBV DNA polymerase mutations might complicate antiviral therapy with NAs,leading to the generation of drug-resistant viral mutants and disease *** most common substitutions are known to be YMDD-motif mutations, but their prevalence and the influence on antiviral therapy is *** To investigate prevalence of the naturally occurring rtM204I mutations in treatment-na?ve CHB genotype C2 patients and their influence on antiviral *** A total of 410 treatment-na?ve CHB patients infected with HBV genotype C2 strains were enrolled in this retrospective study. Among the 410 patients, 232 were treated with NAs for at least 12 mo. Significant fibrosis was defined as fibrosis-4 index > 3.25 or aspartate aminotransferase to platelet ratio index > *** viral response(CVR) during NAs was defined as undetectable serum HBV DNA(< 24 IU/m L). The rtM204I variants were analyzed by a newly developed locked nucleotide probe(LNA probe) based real-time PCR(LNA-RTPCR) *** The LNA-RT-PCR could discriminate rtM204I mutant-type(17 patients, 4.2%)from rt M204 wild-type(386 patients, 95.8%) in 403 of 410 patients(98.3%sensitivity). Multivariate analysis showed that naturally occurring rtM204I variants were more frequently detected in patients with significant fibrosis [oddratio(OR) 3.397, 95% confidence-interval(CI) 1.119-10.319, P = 0.031]. Of 232 patients receiving NAs, multivariate analysis revealed that achievement of CVR was reversely associated with naturally occurring rtM204I variants prior to NAs treatment(OR 0.014, 95%CI 0.002-0.096, P < 0.001). Almost patients receiving tenofovir achieved CVR at 12 mo of tenofovir, irrespective of pre-existence of naturally occurring rtM204I mutations(CVR rates: patients with

关键词： Chronic hepatitis B Entecavir Hepatitis B virus Liver fibrosis Mutation Tenofovir

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Assessing significant fibrosis using imaging-based elastography in chronic hepatitis B patients: Pilot study

引用

World Journal of Gastroenterology 2019年第25期25卷 3256-3267页

作者： Hee Sun Park won hyeok choe Hye Seung Han Mi Hye Yu Young Jun Kim Sung Il Jung Jeong Han Kim So Young Kwon Department of Radiology Konkuk University School of MedicineSeoul 05030South Korea Department of Internal Medicine Konkuk University School of MedicineSeoul 05030South Korea Department of Pathology Konkuk University School of MedicineSeoul 05030South Korea

BACKGROUND Accurate detection of significant fibrosis(fibrosis stage 2 or higher on the METAVIR scale)is important especially for chronic hepatitis B(CHB)patients with high viral loads but with normal or mildly elevated alanine aminotransferase(ALT)levels because the presence of significant fibrosis is accepted as the indication for antiviral *** biopsy is the reference standard for diagnosing significant fibrosis,but it is an invasive ***,noninvasive imaging-based measurements,such as magnetic resonance elastography(MRE)or two-dimensional shear-wave elastography(2DSWE),have been proposed for the quantitative assessment of liver *** To explore MRE and 2D-SWE to identify fibrosis stage,and to compare their performance with that of serum-based *** The study enrolled 63 treatment-na?ve CHB patients with high viral loads but with normal or mildly elevated ALT levels who underwent liver biopsy before a decision was made to initiate antiviral *** and 2D-SWE were performed,and serum-based indices,such as FIB-4 and aspartate transaminase to platelet ratio index(APRI),were *** diagnostic performances of MRE,2D-SWE,FIB-4,and APRI for assessing significant fibrosis(≥F2)and cirrhosis(F4)were evaluated with liver histology as the reference standard,using receiver operating characteristic *** The liver fibrosis stage was F0/F1 in 19,F2 in 14,F3 in 14,and F4 in 16 patients,*** significantly discriminated F2 from F0/1(P=0.022),whereas 2D-SWE showed a broad overlap in distinguishing those *** showed a higher correlation coefficient value with fibrosis stage than 2D-SWE with fibrosis stage(0.869 vs 0.649,Spearman test;P<0.001).Multivariate linear regression analyses showed that fibrosis stage was the only factor affecting the values of MRE(P<0.001),whereas body mass index(P=0.042)and fibrosis stage(P<0.001)were independent factors affecting 2D-SWE *** performance for diagnos

关键词： Antiviral therapy Chronic hepatitis B Liver fibrosis Magnetic resonance elastography Ultrasound elastography

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rapid re-emergence of YMDD mutation of hepatitis B virus with hepatic decompensation after lamivudine retreatment

引用

World Journal of Gastroenterology 2008年第27期14卷 4416-4419页

作者： So Young Kwon won hyeok choe Chang Hong Lee Jong Eun Yeon Kwan Soo Byun Department of Internal Medicine Konkuk University School of Medicine Department of Internal Medicine Korea University College of Medicine

Lamivudine has a high rate of antiviral resistance. Sequential treatment of anti-hepatitis B virus (HBV) is commonly used for lamivudine resistance. We report 4 cases of patients with rapid redetection of HBV mutants during the lamivudine retreatment. The four patients received lamivudine as an initial treatment of HBV and adefovir and lamivudine as a rescue therapy consecutively. HBV-DNA level, YMDD mutations and adefovir -resistant mutations (RFMP) were tested every 3 mo during the sequential treatment. All the patients showed YMDD mutations during the initial lamivudine therapy. After adefovir therapy for lamivudine resistance, they showed viral breakthrough. Adefovir was switched to lamivudine, however, it did not induce viral suppression at all, rather increased HBV-DNA with rapid reemergence of the YMDD mutations. All the patients had ALT flares, and hepatic decompensation occurred in two patients. After switching to adefovir combined with entecavir or lamivudine for a rescue therapy, the patients had reduction in HBV-DNA and ALT improvement. These cases demonstrated that lamivudine retreatment of patients with preexposed lamivudine resistance leads to rapid reemergence of YMDD mutation with significant viral rebounds and subsequent hepatic decompensation. Sequential administration of lamivudine in patients with a prior history of YMDD mutation should be abandoned.

关键词： Hepatitis B Lamivudine Adefovir dipivoxil Mutations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clonal evolution of hepatitis B virus polymerase gene mutations during lamivudine-adefovir combination treatment

引用

World Journal of Gastroenterology 2012年第44期18卷 6437-6446页

作者： Soon Young Ko Byung Kook Kim So Young Kwon Kyun-Hwan Kim Jeong Han Kim won hyeok choe Chang Hong Lee Department of Internal Medicine Konkuk University School of MedicineSeoul 143-729South Korea Department of Pharmacology Konkuk University School of MedicineSeoul 143-729South Korea

AIM:To identify hepatitis B virus polymerase gene mutations during antiviral therapy using lamivudineadefovir sequential monotherapy followed by lamivudine-adefovir combination ***:The patient cohort included four adult chronic hepatitis B patients who had undergone sequential monotherapy,first with lamivudine(LMV) and then,after developing viral breakthrough,with adefovir(ADV) *** of the patients had non-response or viral breakthrough after LMV-ADV sequential monotherapy,which resulted in the switching of their antiviral regimen to LMV-ADV combination *** serum samples from the four patients who showed non-response to rescue LMV-ADV combination therapy were collected sequentially at a time before the antiviral treatment and then during the LMV monotherapy,ADV monotherapy,and LMV-ADV combination *** the genotypic analysis,the whole 1310-bp polymerase gene region was amplified,cloned and ***:All patients had been previously treated with 100 mg of LMV once daily for a 15-to 26-mo *** emergence of resistance mutations to LMV,such as rtM204V/I and/or rtL180M,were found in all *** antiviral regimens were switched to ADV monotherapy as the second line *** patients had viral breakthrough or non-response after the LMV-ADV sequential ***-resistant mutations were detected after 13 to 19 mo of LMV-ADV sequential *** rtA181V/T mutations were predominantly identified during the ADV treatment in the LMV-resistant ***-seven of 38 clones were combined with an amino acid change at rt181;three clones had mutations in rt236 and one clone had a combined *** rtA181V/T mutations were not suppressed by the LMV-ADV combination ***-nine of 64 clones showed an rtA181V/T mutation and six clones showed combined mutations in rt181 and *** in rt204 re-emerged during the combination *** rt181 and rt204 mutations did not co-exist in one clon

关键词： Hepatitis B virus Lamivudine Adefovir Mutation Drug resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：