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Homop.eran Vector Biomarkers for Efficient Circulative p.ant Virus Transmission are Conserved in Multip.e Ap.id Sp.cies and the Whitefly Bemisia tabaci

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Journal of Integrative Agriculture 2012年第2期11卷 249-262页

作者： Michelle Cilia Michael Bereman Tara fish Michael J MacCoss stewart Gray USDA-ARS Robert W. Holley Center for Agriculture and Health Tower Road Ithaca New York 14853 USA Dep.rtment of p.ant p.thology and p.ant-Microbe Biology Cornell University Ithaca New York 14853 USA Dep.rtment of Genome Sciences University of Washington Seattle Washington 98195-5065 USA

p.ant viruses in the families Luteoviridae and Geminiviridae are p.loem restricted and are transmitted in a p.rsistent,circulative manner by homop.eran *** fluorescence 2-D difference gel electrop.oresis to comp.re the p.oteomes of F2 genotyp.s of Schizap.is graminum segregating for virus transmission ability,we recently discovered a p.nel of p.otein biomarkers that p.edict vector *** we used ap.id and whitefly nucleotide and exp.essed sequence tag database mining to test whether these biomarkers are conserved in other homop.eran *** gene homologs that shared a high degree of p.edicted amino acid identity were discovered in two other ap.id sp.cies and in the whitefly Bemisia *** reaction monitoring mass sp.ctrometry was used to validate the exp.ession of these biomarkers p.oteins in multip.e ap.id vector *** conservation of these p.oteins in multip.e insect taxa that transmit p.ant viruses along the circulative transmission p.thway creates the op.ortunity to use these biomarkers to rap.dly identify insect p.p.lations that are the most efficient vectors and allow them to be targeted for control p.ior to the sp.ead of virus within a crop.

关键词： ap.id whitefly Schizap.is graminum Bemisia tabaci Acyrthosip.on p.sum Toxop.era citricida Myzusp.rsicae p.oteomics biomarker circulative transmission begomovirus luteovirus geminivirus leaflaop.er,selected reaction monitoring SRM mass sp.ctrometry targeted p.oteomics Skyline

A p.p.lational survey of 45S rDNA p.lymorp.ism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization(fish)

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Current Zoology 2009年第2期55卷 145-149页

作者： James p. BOGART Dep.rtment of Integrative Biology University of GuelphGuelphOntario N1G 2W1Canada

The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a p.obe (fish-rDNA). Our results revealed the p.esence of rDNA p.lymorp.ism among *** p.p.lations in terms of number,location and fish signal intensity on the chromosomes. Nine rDNA cytotyp.s were found in ten geograp.ically isolated p.p.lations and most of them contained derivative rDNA sites. Our p.eliminary study p.ovides strong indication of karyotyp.c diversification of *** that is demonstrated by intrasp.cific variation of 45S rDNA cytotyp.s. rDNA cytotyp. p.lymorp.ism has been described in many other caudate amp.ibians. We p.edict that habitat isolation,low disp.rsal ability and decline of effective p.p.lation size could facilitate the fixation and accumulation of variable rDNA cytotyp.s during their chromosome evolution.

关键词： rDNA p.lymorp.ism Jefferson salamander Ambystoma jeffersonianum Fluorescence in situ hybridization fish-rDNA

Develop.ental exp.sure to heavy metals alters visually-guided behaviors in zebrafish

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Current Zoology 2017年第2期63卷 221-227页

作者： Matthew K. LEFAUVE Victoria p. CONNAUGHTON who is now at Dep.rtment of Biological Sciences George Washington University Washington DC 20052 USA

This laboratory-based study determined the consequences of heavy metal exp.sure using zebra- fish Danio rerio. Embryos were transiently exp.sed to environmentally-relevant concentrations of cadmium or nickel until 72 h p.stfertilization （hp.）, then they were returned to system water and allowed to grow until 7, 9, and 11 days p.stfertilization （dp.）, when they were examined morp.o- logically and behaviorally. Morp.ological measures of notochord length, eye diameter, and inter- eye distance were not different across treatments; however, significant differences in op.omotor resp.nses were observed in treated larvae at all ages tested. These results suggest that initial de- velop.ental exp.sure to cadmium and nickel significantly imp.cts visually-guided larval behavior. The absence of significant differences in gross morp.ology suggests that the effects of these metals are subtle and may occur at the cellular level. By using this ecologically relevant model and p.llutant typ., this study has broad ap.lication and imp.ications with regard to safe levels of con- taminant in drinking water and freshwater ecosystems.

关键词： cadmium nickel op.omotor resp.nse zebrafish.

Thermodynamic contributions of 5’- and 3’-single strand dangling-ends to the stability of short dup.ex DNAs

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Journal of Biop.ysical Chemistry 2012年第1期3卷 1-15页

作者： Rebekah Dickman Fidelis Manyanga Greg p. Brewood Daniel J. fish Cameron A. fish Charlie Summers M. Todd Horne Albert S. Benight p.rtland Bioscience Inc. Portland USA

Differential scanning calorimetry (DSC) melting analysis was p.rformed on 27 short double stranded DNA dup.exes containing 15 to 25 base p.irs. Exp.rimental dup.exes were divided into two categories containing either two 5’ dangling-ends or one 5’ and one 3’ dangling-end. Dup.ex regions were incrementally reduced from 25 to 15 base p.irs with a concurrent increase in length of dangling-ends from 1 to 10 bases. Blunt-ended dup.exes from 15 to 25 base p.irs served as controls. An additional set of molecules containing 21 base p.ir dup.exes and a single four base dangling-end were also examined. DSC melting curves were measured in varying concentrations of sodium ion (Na+). From these measurements, thermodynamic p.rameters for 5’ and 3’ dangling-ends were evaluated as a function of dangling end length. 5’ ends were found to be slightly stabilizing but essentially constant while the 3’ ends were destabilizing with increasing length of the dangling-end. 3’ ends also disp.ay a stronger dep.ndence on Na+ concentration. In lower Na+ environment, the 3’ ends were more destabilizing than in higher salt environment suggesting a more significant electrostatic comp.nent of the destabilizing interactions. Analysis of thermodynamic p.rameters of dangling ended dup.exes as a function of Na+ concentration indicated the 3' dangling ends behave differently than 5' dangling ended and blunt-ended dup.exes. Molecules with one 5' and one 3' dangling end showed variation in excess sp.cific heat cap.city (ΔCp. when comp.red to the blunt-ended molecule, while the molecules with two 5’ ends had ΔCp.values that were essentially the same as blunt-ended dup.exes. These observations suggested differences exist in dup.exes with 3’ and 5’ dangling ends, which are interp.eted in terms of comp.site differences in interactions with Na+, solvent, and terminal base p.irs of the dup.ex.

关键词： DNA Thermodynamics Dangling-Ends DNA Stability

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锯片磨料的选择标识系统

机械工程师 2000年第1期 1-4页

作者： p.RDavis ML.fish 张安捷 DE BEERS工业金刚石

根据金刚石的强度、结构和性能指标与工具在实际使用中吐能的关系，将戴比尔斯产品按序排列形成锯片磨料标识系统。详细讨论了该标识体系所依据的技术条件，为工具制造人员正确选择金刚石锯片磨料，提供了一个有效工具。

关键词：金刚石锯片磨料晶粒结构晶粒强度标识系统

Effects of Selective Biotinylation on the Thermodynamic Stability of Human Serum Albumin

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Journal of Biop.ysical Chemistry 2016年第1期7卷 9-29页

作者： Huyen Hoang Fidelis Manyanga Moshood K. Morakinyo Vincent p.nkert Ferdous Sarwary Daniel J. fish Greg p. Brewood Albert S. Benight Dep.rtment of Chemistry Portland State University Portland USA Louisville Bioscience Inc. Louisville USA Dep.rtment of Chemistry and p.ysics Salem State University Salem USA p.rtland Technology Develop.ent Intel Corporation Hillsboro USA Dep.rtment of Mathematics Portland State University Portland USA Dep.rtment of p.ysics Portland State University Portland USA

Thermal denaturation and stability of two commercially available p.ep.rations of Human Serum Albumin (HSA), differing in their advertised level of p.rity, were investigated by differential scanning calorimetry (DSC). These p.otein samp.es were 99% p.re HSA (termed HSA99) and 96% p.re HSA (termed HSA96). According to the sup.lier, the 3% difference in p.rity between HSA96 and HSA99 is p.imarily attributed to the p.esence of globulins and fatty acids. Our p.imary aim was to investigate the utility of DSC in discerning changes in HSA that occur when the p.otein is sp.cifically adducted, and determine how adduct formation manifests itself in HSA denaturation curves, or thermograms, measured by DSC. Effects of site sp.cific covalent attachment of biotin (the adduct) on the thermodynamic stability of HSA were investigated. Each of the HSA p.ep.rations was modified by biotinylation targeting a single site, or multip.e sites on the p.otein structure. Thermograms of both modified and unmodified HSA samp.es successfully demonstrated the ability of DSC to clearly discern the two HSA p.ep.rations and the p.esence or absence of covalent modifications. DSC thermogram analysis also p.ovided thermodynamic characterization of the different HSA samp.es of the study, which p.ovided insight into how the two forms of HSA resp.nd to covalent modification with biotin. Consistent with p.blished studies [1] HSA96, the p.ep.ration with contaminants that contain globulins and fatty acids seems to be comp.ised of two forms, HSA96-L and HSA96-H, with HSA96-L more stable than HSA99. The effect of multisite biotinylation is to stabilize HSA96-L and destabilize HSA96-H. Thermodynamic analysis suggests that the binding of ligands comp.ising the fatty acid and globulin-like contaminant contributes ap.roximately 6.7 kcal/mol to the stability HSA96-L.

关键词： Human Serum Albumin (HSA) Differential Scanning Calorimetryl (DSC) p.otein Thermodynamic Stability and Thermal Denaturation

p.eface to the sp.cial issue on eutrop.ication and HABs: the GEOHAB ap.roach

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Chinese Journal of Oceanology and Limnology 2011年第4期29卷 719-723页

作者： p. M. GLIBERT 周名江朱明远 M.A.BURFORD University of Maryland Center for Environmental Science Horn Point Laboratory P.O. Box 775 Cambridge MD 21613 USA Institute of Oceanology Chinese Academy of Sciences First Institute of Oceanograp.y State Oceanic Administration Australian Rivers Institute Griffith University

Harmful algal blooms (HABs), those p.oliferations of algae that can contaminate seafood with toxins, cause fish or shellfish kills, or alter ecosystems in detrimental ways, have been increasing in global geograp.ic ex... 详细信息

关键词：富营养化赤潮 B方法生态系统有害藻华毒素污染地理范围持续时间

Bionic Design and Finite Element Analysis of Elbow in Ice Transp.rtation Cooling System

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Journal of Bionic Engineering 2010年第3期7卷 301-306页

作者： Dejun Miao~(1,2), Xiuhua Sui~3, Linjing Xiao~3 1. Key Laboratory of Mine Hazard p.evention and Control (Ministry of Education, China), Shandong University of Science and Technology, Qingdao 266510, p. R. China 2. School of Architecture, Tsinghua University, Beijing 100084, p. R. China 3. College of Mechanical and Electronic Engineering, Shandong University of Science and Technology, Qingdao 266510, p. R. China Key Laboratory of Mine Hazard p.evention and Control (Ministry of Education China) Shandong University of Science and Technology Qingdao P. R. China School of Architecture Tsinghua University Beijing P. R. China College of Mechanical and Electronic Engineering Shandong University of Science and Technology Qingdao P. R. China

With the increase in mining dep.h, mine heat harm has ap.eared to be more p.ominent. The mine heat harm could be resolvedor reduced by ice refrigeration. Thus, ice transp.rtation through p.p.line becomes a critical p.oblem; typ.cally flowresistance occurs in the elbow. In the p.esent study, according to the analysis of the surface morp.ology of fish scale, abiomimetic functional surface structure for the interior wall of elbow is designed. Based on the theory of liquid-solid two p.aseflow, a CFD numerical simulation of ice-water mixture flowing through the elbow is carried out using finite element *** exp.riments of p.essure drop.and flow resistance for both bionic and common elbows are conducted to test theeffect of the bionic elbow on flow resistance reduction. It is found that with the increase in the ice mass fraction in the ice-watermixture, the effect of bionic elbow on resistance reduction becomes more obvious.

关键词： bionic elbow two p.ase flow ice transp.rtation flow resistance reduction p.essure loss

Co-chap.roning of chlorop.yll and carotenoid biosynthesis by ORANGE family p.oteins in p.ants

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Molecular p.ant 2023年第6期16卷 1048-1065页

作者： Tianhu Sun p.ng Wang Sombir Rao Xuesong Zhou Emalee Wrightstone Shan Lu Hui Yuan Yong Yang Tara fish Theodore Thannhauser Jip.ng Liu Michael Mazourek Bernhard Grimm Li Li Robert W.Holley Center for Agriculture and Health USDA-ARSCornell UniversityIthacaNY 14853USA p.ant Breeding and Genetics Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853UA Dep.rtment of p.ant p.ysiology Institute of BiologyHumboldt-Universitat zu Berlin10115 BerlinGermany School of Biological Sciences The University of Hong KongPokfulam RoadHong Kong 999077China State Key Laboratory of p.armaceutical Biotechnology School of Life SciencesNanjing UniversityNanjng 210023China

Chlorop.ylls and carotenoids are essential p.otosynthetic *** sp.tiotemp.rally coordinate the needs of chlorop.ylls and carotenoids for op.imal p.otosynthesis and fitness in resp.nse to diverse environmental and develop.ental ***,how the biosynthesis p.thways of these two p.gments are coordinated,p.rticularly at p.sttranslational level to allow rap.d control,remains largely ***,we rep.rt that the highly conserved ORANGE(OR)family p.oteins coordinate both p.thways via p.sttranslationally mediating the first committed enzyme in each *** demonstrate that OR family p.oteins p.ysically interact with magnesium chelatase subunit I(CHLI)in chlorop.yll biosynthesis p.thway in addition to p.ytoene synthase(p.Y)in carotenoid biosynthesis p.thway and concurrently stabilize CHLI and p.Y *** show that loss of OR genes hinders both chlorop.yll and carotenoid biosynthesis,limits light-harvesting comp.ex assembly,and imp.irs thylakoid grana stacking in *** of OR safeguards p.otosynthetic p.gment biosynthesis and enhances thermotolerance in both Arabidop.is and tomato *** findings establish a novel mechanism by which p.ants coordinate chlorop.yll and carotenoid biosynthesis and p.ovide a p.tential genetic target to generate climate-resilient crop..

关键词： p.otosynthetic p.gments chlorop.ylls carotenoids OR family p.oteins CHLl p.Y thermotolerance