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Microcomputer hardware, software, and troubleshooting for engineering and technology / James L. Antonakos, Kenneth C. mansfield.

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Endo-β-1,4-glucanases impact plant cell wall development by influencing cellulose crystallization

2000年

作者： James L. Antonakos Kenneth C. mansfield.

来源：畅想之星图书光盘版（南... 评论

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Journal of Integrative Plant Biology 2015年第4期57卷 396-410页

作者： Magdalena Glass Sarah Barkwill Faride Unda Shawn D.mansfield Department of Wood Science University of British Columbia

Cell walls are vital to the normal growth and development of plants as they protect the protoplast and provide rigidity to the stem. Here, two poplar and Arabidopsis orthologous endoglucanases, which have been proposed to play a role in secondary cell wall development, were examined. The class B endoglucanases, Pt GH9B5 and At GH9B5, are secreted enzymes that have a predicted glycosylphosphatidylinositol anchor, while the class C endoglucanases, Pt GH9C2 and At GH9C2, are also predicted to be secreted but instead contain a carbohydrate-binding *** poplar endoglucanases were expressed in Arabidopsis using both a 35 S promoter and the Arabidopsis secondary cell wall-specific Ces A8 promoter. Additionally, Arabidopsis t-DNA insertion lines and an RNAiconstruct was created to downregulate At GH9C2 in Arabidopsis. All of the plant lines were examined for changes in cell morphology and patterning, growth and development, cell wall crystallinity, micro fibril angle, and proportion of cell wall carbohydrates. Misregulation of Pt GH9B5/At GH9B5 resulted in changes in xylose content, while misregulation of Pt GH9C2/At GH9C2 resulted in changes in crystallinity, which was inversely correlated with changes in plant height and rosette diameter. Together, these results suggest that these endoglucanases affect secondary cell wall development by contributing to the cell wall crystallization process.

关键词： Cellulose cell walls crystallinity endoglucanase expansion glucose xylose

Psychotropic Medication Refusal:Reasons and Patients’Perception at a Secure Forensic Psychiatric Treatment Centre

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Journal of Forensic Science and Medicine 2016年第1期2卷 12-17页

作者： Olajide O Adelugba mansfield Mela Inam U Haq Regional Psychiatric Center University of SaskatchewanSaskatoonSaskatchewanCanada Department of Psychiatry University of SaskatchewanSaskatoonSaskatchewanCanada

Poor adherence to prescribed medication regimens can undermine the effectiveness of *** study was conducted to determine the demographic profile of forensic psychiatric inpatients refusing medications and to identify the reasons for *** were collected through interviews using a questionnaire including Drug Attitude Inventory‑*** refusal was more common among Aboriginals(68%,n=34)than Caucasians(32%,n=16)and was highest among the patients 21–30 years of age(44%,n=22).Antisocial personality disorder and substance use disorder featured prominently among patients refusing *** main reasons for medication refusal were inconvenience(34%,n=17)followed by side effects(22%,n=11),ineffective medication(20%,n=10),illness‑related(16%,n=8),and no reasons(8%,n=6).Antipsychotic medications topped the list of the major classes of medications refused followed by Antidepressants and Mood Stabilizers.

关键词： Forensic medication nonadherence psychiatry refusal

Management of hilar cholangiocarcinoma in the North of England: Pathology, treatment, and outcome

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World Journal of Gastroenterology 2005年第48期11卷 7625-7630页

作者： SD mansfield O Barakat RM Charnley BC Jaques CB O'Suilleabhain PJ Atherton D Manas Department of Hepato-Pancreatico-Biliary Surgery

AIM: To assess the management and outcome of hilar cholangiocarcinoma (Klatskin tumor) in a single tertiary referral ***: The notes of all patients with a diagnosis of hilar cholangiocarcinoma referred to our unit for over an 8-year period were identified and retrospectively reviewed. Presentation, management and outcome were ***: Seventy-five patients were identified. The median age was 64 years (range 34-84 years). Male to female ratio was 1:1. Eighty-nine percent of patients presented with jaundice. Most patients referred were under Bismuth classification 3a, 3b or 4. Seventy patients required biliary drainage, 65 patients required 152percutaneous drainage procedures, and 25 had other complications. Forty-one patients had 51 endoscopic drainage procedures performed (15 failed). Of these,36 subsequently required percutaneous drainage. The median number of drainage procedures for all patients was three, 18 patients underwent resection (24%), nine had major complications and three died *** 5-year survival rate was 4.2% for all patients, 21%for resected patients and 0% for those who did not undergo resection (P = 0.0021). The median number of admissions after diagnosis in resected patients was two and three in non-resected patients (P＜0.05).Twelve patients had external-beam radiotherapy, seven brachytherapy, and eight chemotherapy. There was no significant benefit in terms of survival (P = 0.46) or hospital ***: Resection increases survival but carries the risk of significant morbidity and *** biliary drainage is almost always necessary and endoscopic drainage should be avoided if possible.

关键词： Hilar cholangiocarcinoma Pathology Treatment Outcome England

Manipulating Chladni Patterns of Ferromagnetic Materials by an External Magnetic Field

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Sound & Vibration 2021年第3期55卷 235-240页

作者： Kenneth R.Podolak Vihan A.W.Wickramasinghe Gareth A.mansfield Alex M.Tuller State University of New York Plattsburgh12901USA

Ernst Chladni is called the father of acoustics for his work,which includes investigating patterns formed by vibrating *** these patterns helps research involving standing waves and other harmonic behaviors,including studies of single electron orbits in *** experiment vibrates circular plates which result in well-known *** to traditional experiments that used sand or salt,we use magnetic materials,namely iron filings and nickel *** then manipulate the patterns by applying a localized external magnetic field to one of the rings that moves a segment of the magnetic material in that ring to the next inner *** results show a significant decrease in magnetic field necessary to move the magnetic material at higher frequencies as well as a significant decrease in the magnetic field required to move the magnetic material as nickel powder is substituted for iron filings while keeping the mass constant.

关键词： Chladni ferromagnetism acoustics

Effects of intracellular application of CsCL on action potential and contractio,

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中国药理学与毒理学杂志 1995年第1期9卷 73-74页

作者： CUI Yi Derek A TERRAR(University Department of Pharmacology, mansfield jRoad,Oxford OX13QT,UK) Univ. Department of Pharmacology Mansfield Road Oxford OX1 3QT United Kingdom

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关键词： myocardium, sarcoplasmicreticulum, ion channels, K+ contraction, cesium

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Sun and Moon

英语学习 1994年第3期 3-9页

作者： Katherine mansfield 乐安

In the afternoon the chairs came,a whole big cart full of lit-tle gold ones with their legs in the *** then the *** you stared down from the balcony at the people car-rying them the flower pots looked like funny awfully nice hatsnodding up the *** thought they were *** said:"***'s aman wearing a palm on his head."But she never knew the differ-

关键词： down over

Characterisation of Thin Films Using a Coherence Scanning Interferometry

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Journal of Materials Science and Chemical Engineering 2015年第1期3卷 15-21页

作者： Yang Yu Daniel mansfield Taylor Hobson Ltd. PO Box 36 2 New Star Road Leicester LE4 9JQ UK

Accurate measurement of film thickness and its uniformity are very important to the performance of many coated surfaces and for many applications are critical. Effective inspection of the film thickness and uniformity is the key to high performance. Conventionally, film thickness is meas- ured using a spectrophotometer/reflectometer, ellipsometer or a physical step measurement;however, these techniques all have limitations. Coherence Scanning Interferometry (CSI) is an established method to measure surface topography as this technique offers many advantages such as speed, ease of use and accuracy. The measurement of “thick” films (exceeding ~1.5 μm) which gives rise to clearly separate fringe bunches is a well-established CSI capability. However, a methodology known as the ‘helical complex field’ (HCF) [1] [2] function allows film thickness to be measured down to ~25 nm. This new method, combined with Coherence Correlation Interferometry (CCI) [3] offers film thickness measurements with sub-nanometre vertical resolution and ~1 μm lateral resolution. It is ideally suited for detailed analysis of coated optical surfaces. In this paper, the fundamentals of the techniques are described and some case studies are presented.

关键词： Film Thickness CCI Thick Film HCF

Initial Validation of Cytokine Measurement by ELISA in Canine Feces

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Open Journal of Veterinary Medicine 2013年第6期3卷 282-288页

作者： Nathalee Prakash Phil Stumbles Caroline mansfield School of Veterinary and Biomedical Sciences Murdoch University Perth Australia

Measurement of fecal cytokines has been used as a marker of intestinal inflammation in people and correlates with endoscopic findings. The aim of this study was to evaluate the use of canine-specific enzyme-linked immunosorbant assays (ELISAs) for quantification of cytokines in canine fecal samples as a non-invasive biomarker. Interleukin (IL)-6, -8, -10, -23/12p40 and TNF-a were assessed by using spiked fecal samples from 3 healthy dogs. Standard curve validation was performed, and the impact of time to freeze, duration of storage and number of freeze-thaw cycles on cytokine concentration were also examined. All the cytokines assayed could be detected, with varying accuracy. The mean coefficient of variation (CV) for all standard curves ranged from 2.95%-9.8%. The mean intra-assay CV ranged from 3.1%-11.14%, and inter-assay CV from 4.36%-18.83%. Recovery of IL-23 was poor (7.23%-17.12%), precluding further interpretation of stability studies. Mean recovery did not appear to be affected by time to freeze and repeat freeze-thaw cycles in all cytokines investigated. Recovery for all cytokines after short-term storage of 30 days at -80℃ showed a recovery of 70% or >130%. In conclusion, although fecal IL-6, -8, -10, and TNF-a could be used as biomarkers of intestinal inflammation in the dog, the quality of laboratory performance and poor recovery at lower concentrations limit their application. Bench-top and freeze-thaw stability was acceptable, and samples should ideally be analyzed within a week. Investigation involving dogs with acute and chronic inflammatory intestinal disease is required to determine the role of this methodology in a clinical setting.

关键词： Fecal Cytokines ELISA Validation Canine Enteritis