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Phase transition of multi-component(TiZrVNb)C ceramics—Part II:From single phase to multiple phases via adjusting V content

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Journal of Advanced Ceramics 2024年第5期13卷 689-698页

作者： Qingyi Kong Lei Chen Sijia Huo Kunxuan Li wenyu lu Yujin Wang Yu Zhou Institute for Advanced Ceramics School of Materials Science and EngineeringHarbin Institute of TechnologyHarbin 150080China Key Laboratory of Advanced Structure‒Function Integrated Materials and Green Manufacturing Technology Harbin Institute of TechnologyHarbin 150001China National Key Laboratory of Precision Hot Processing of Metals Harbin Institute of TechnologyHarbin 150001China

To address the relatively mediocre mechanical properties of single-phase multi-component carbide ceramics,a phase transition from a single phase to multiple phases was proposed to achieve superior mechanical properties.A series of(TiZrVxNb)C0.8 ceramics with different V contents were fabricated by spark plasma sintering(SPS).The influence of the V content on the phase composition,microstructural evolution,and mechanical properties was investigated in detail.The transition behavior from a single phase to multiple phases is discovered and discussed.The formation of the Zr-rich phase and Zr-poor phase can be attributed to the increase in lattice distortion and mixed enthalpy caused by the addition of V.A nanometer lamellar structure with a semi-coherent interface obtained via in situ decomposition is reported for the first time in multi-component carbide ceramics.The semi-coherent interfaces with high dislocation density and strain concentration effectively improve the mechanical properties,grain refinement,and multi-phase formation.The optimal comprehensive mechanical properties of the Vickers hardness(26.3 GPa),flexural strength(369 MPa),and fracture toughness(3.1 MPa·m1/2)were achieved for the sample with 20 mol%V.

关键词： multi-component ceramics multiple phases microstructure evolution phase decomposition mechanical properties

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Phase transition of multi-component(TiZrVNb)C ceramics—Part I:Phase decomposition induced by carbon content

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Journal of Advanced Ceramics 2024年第5期13卷 679-688页

作者： Qingyi Kong Rao Zhang Lei Chen Sijia Huo wenyu lu Yujin Wang Boxin Wei Yu Zhou Institute for Advanced Ceramics School of Materials Science and EngineeringHarbin Institute of TechnologyHarbin 150080China Key Laboratory of Advanced Structure‒Function Integrated Materials and Green Manufacturing Technology Harbin Institute of TechnologyHarbin 150001China School of Materials Science and Chemical Engineering Harbin University of Science and TechnologyHarbin 150001China National Key Laboratory of Precision Hot Processing of Metals Harbin Institute of TechnologyHarbin 150001China

Phase decomposition can effectively enhance the mechanical properties of carbide ceramics and can overcome the difficulty of enhancing the mechanical properties of single-phase multicomponent carbide ceramics.In this work,a series of nonstoichiometric(TiZrVNb)Cx ceramics were prepared by spark plasma sintering(SPS)at different temperatures.The effects of the carbon content on the phase composition,microstructure evolution,and mechanical properties were investigated in detail.Phase decomposition occurred with decreasing carbon content.Two different solid solutions of(Ti,V)-rich and Zr-rich phases formed from the decomposition of equimolar single-phase solid solutions,namely,the Zr-poor phase and Zr-rich phase,respectively.The distribution of Nb element is relatively uniform.The semicoherent interfaces between the Zr-poor phase and the Zr-rich phase can harden and strengthen effectively under the synergistic effect of grain refinement.Ceramics with phase decomposition structures have apparent advantages compared to single-phase high-entropy carbides.This work provides an important train of thought for the microstructure tailoring and properties optimization of multi-component carbide ceramics.

关键词： multicomponent ceramics nontoichiometry microstructure evolution phase decomposition mechanical properties

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Low thermal conductivity of dense(TiZrHfVNbTa)C_(x) high-entropy carbides by tailoring carbon stoichiometry

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Journal of Advanced Ceramics 2023年第1期12卷 49-58页

作者： Lei CHEN Wen ZHANG wenyu lu Boxin WEI Sijia HUO Yujin WANG Yu ZHOU Institute for Advanced Ceramics School of Materials Science and EngineeringHarbin Institute of TechnologyHarbin 150001China Key Laboratory of Advanced Structural–Functional Integration Materials&Green Manufacturing Technology Harbin Institute of TechnologyHarbin 150001China School of Materials Science and Chemical Engineering Harbin University of Science and TechnologyHarbin 150001China

Transition metal carbides are promising candidates for thermal protection materials due to their high melting points and excellent mechanical properties.However,the relatively high thermal conductivity is still a major obstacle to its application in an ultra-high-temperature insulation system.In this work,the low thermal conductivity of dense(TiZrHfVNbTa)Cx(x=0.6-1)high-entropy carbides has been realized by adjusting the carbon stoichiometry.The thermal conductivity gradually decreases from 10.6 W·m^(−1)·K^(−1) at room temperature to 6.4 W·m^(−1)·K^(−1) with carbon vacancies increasing.Due to enhanced scattering of phonons and electrons by the carbon vacancies,nearly full-dense(97.9%)(TiZrHfVNbTa)C_(0.6) possesses low thermal conductivity of 6.4 W·m^(−1)·K^(−1),thermal diffusivity of 2.3 mm^(2)·s^(−1),as well as electrical resistivity of 165.5μΩ·cm.The thermal conductivity of(TiZrHfVNbTa)C_(0.6) is lower than that of other quaternary and quinary high-entropy carbide ceramics,even if taking the difference of porosity into account in some cases,which is mainly attributed to compositional complexity and carbon vacancies.This provides a promising route to reduce the thermal conductivity of high-entropy carbides by increasing the number of metallic elements and carbon vacancies.

关键词： high-entropy carbides thermal conductivity compositional complexity carbon vacancies

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杭州国家版本馆

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建筑学报 2024年第3期 46-53页

作者：无吉云(摄影) 业余建筑工作室不详

业主杭州国家版本馆设计团队陈立超、宋曙华、申屠团兵、赵远鹏、陈永兵、黄迪、汤恒、陈春吉,等初步设计中国美术学院风景建筑设计研究总院有限公司施工图配合设计浙江省建筑设计研究院全过程咨询中国联合工程有限公司基地面积 8.68...

业主杭州国家版本馆设计团队陈立超、宋曙华、申屠团兵、赵远鹏、陈永兵、黄迪、汤恒、陈春吉,等初步设计中国美术学院风景建筑设计研究总院有限公司施工图配合设计浙江省建筑设计研究院全过程咨询中国联合工程有限公司基地面积 8.68 hm2建筑面积 10.32万m2结构形式钢筋混凝土框架与钢木混合结构

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Engineering Corynebacterium glutamicum for Geraniol Production

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Transactions of Tianjin University 2021年第5期27卷 377-384页

作者： Man Li Shuo Xu wenyu lu School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China Key Laboratory of System Bioengineering(Tianjin University) Ministry of EducationTianjin 300072China SynBio Research Platform Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin 300072China

Geraniol is a monoterpenoid alcohol with various applications in food,cosmetics,and healthcare.Corynebacterium glutamicum is a potential platform for terpenoids production because it harbors the methylerythritol phosphate pathway.To engineer C.glutamicum to produce geraniol,two different truncated geraniol synthases (GESs) were respectively expressed,and strain LX02 expressing the truncated GESs from Valeriana officinalis (t Vo GES) produced 0.3 mg/L of geraniol.Then,three geranyl diphosphate synthases (GPPSs) were combinatorially co-expressed with t Vo GES to improve geraniol production.The amounts of produced geraniol were all higher than that produced by strain LX02.Strain LX03 co-expressing ERG20 F96W–N127W (ERG20 WW) and t Vo GES produced the highest amount,5.4 mg/L.Subsequently,the co-overexpression of1-deoxy-D-xylulose-5-phosphate synthase (dxs) and isopentenyl diphosphate isomerase (idi) further increased the production to 12.2 mg/L in strain LX03.Lastly,the production of geraniol was increased to 15.2 mg/L via fermentation optimization.To our knowledge,this is the first report on the engineering of C.glutamicum to produce geraniol and thus can serve as a reference for other monoterpenoid production studies.

关键词： Corynebacterium glutamicum Geraniol Geraniol synthase Overexpression Metabolic engineering

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Characterization and analysis of petrochemical wastewater through particle size distribution, biodegradability, and chemical composition

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Chinese Journal of Chemical Engineering 2019年第2期27卷 444-451页

作者： Xiaoqiang Jia Dayao Jin Chen Li wenyu lu Department of Biochemical Engineering School of Chemical Engineering and Technology Tianjin University Key Laboratory of Systems Bioengineering Tianjin University Synthetic Biology Platform Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)

The centralized treatment method is a widely used form of wastewater treatment that tends to be less effective at removing toxic substances. Therefore, a detailed analysis of the composition of wastewater can provide important information for the design of an effective wastewater treatment process. The objective of this paper was to investigate particle size distribution(PSD), biodegradability, and the chemical composition of the petrochemical wastewater discharges. For this purpose, this project selected the petrochemical wastewater and treated wastewater of China National Offshore Oil Corporation Zhongjie Petrochemical Co, Ltd. as the analysis objects.The step-by-step filtration method, along with a molecular weight classification method, was selected to build the chemical oxygen demand(COD) and biochemical oxygen demand(BOD) fingerprints of petrochemical wastewater and treated wastewater. The results showed that the main pollutants were settleable particles in petrochemical wastewater, which contributed to over 54.85% of the total COD. The colloidal particles with particle sizes in the range of 450–1000 nm had the highest COD value in the treated wastewater, which contributed34.17% of the total COD of treated wastewater. The results of the BOD analysis showed that the soluble fractions were the main reason that treated wastewaters did not meet the treatment standards. Tests on the organic compounds in petrochemical wastewater found that there were mainly linear paraffins, branched paraffins, benzene series compounds, and some plasticizers in the influent of the petrochemical wastewater. The most abundant pollutants in treated petrochemical wastewater were the adjacent diisobutyl phthalate and the linear alkanes.Fourier transform infrared(FTIR) transmission spectroscopy analysis showed that the settleable particles of petrochemical wastewater and membrane bioreactor(MBR)-treated wastewater contained multiple types of organic substances. The results also indicated that removing the oil-settleable substances, the

关键词： Petrochemical wastewater Gradient membrane separation Chemical oxygen demand Biochemical oxygen demand

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OsBLS6.2:A rice bacterial leaf streak resistance gene identified by GWAS and RNA-seq

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The Crop Journal 2023年第6期11卷 1862-1871页

作者： Huabin Xie Chunzi Lin wenyu lu Zhikai Han Danhong Wei Xing Huo Tianjiao Li Jian Zhang Yongqiang He Chun Chen Hui Wang Tao Guo Jiafeng Wang National Engineering Research Center of Plant Space Breeding Guangdong Key Laboratory of Plant Molecular BreedingSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Key Laboratory of New Technology in Rice Breeding Guangdong Rice Engineering LaboratoryRice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina Guangxi Key Laboratory of Agro-environment and Agro-products Safety College of AgricultureGuangxi UniversityNanning 530004GuangxiChina

Bacterial leaf streak(BLS),caused by Xanthomonas oryzae pv.oryzicola(Xoc),is a bacterial disease affecting rice production in Asia and Africa,whose severity is expected to increase with climate change.Identification of new quantitative-trait loci(QTL)or resistance genes for BLS resistance is essential for developing resistant rice.A genome-wide association study to identify QTL associated with BLS resistance was conducted using phenotypic and genotypic data from 429 rice accessions.Of 47 QTL identified,45 were novel and two co-localized with previously reported QTL or genes conferring BLS resistance.qBLS6.2 on chromosome 6 explained the greatest phenotypic variation.Combined analysis of differential expression and annotations of predicted genes near qBLS6.2 based on haplotype and disease phenotype identified OsBLS6.2(LOC_Os06g02960)as a candidate gene for qBLS6.2.OsBLS6.2 knockout plants showed higher resistance to Xoc than wild-type plants.Many other candidate genes for resistance to Xoc were identified.

关键词： Rice Bacterial leaf streak Xanthomonas oryzae pv.oryzicola Genome-wide association study RNA sequencing

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Distribution of Bacterial Communities in Petroleum-Contaminated Soils from the Dagang Oilfield, China

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Transactions of Tianjin University 2020年第1期26卷 22-32页

作者： Xueke Feng Zhen Liu Xiaoqiang Jia wenyu lu School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China Key Laboratory of System Bioengineering(Tianjin University) Ministry of EducationTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) SynBio Res PlatformTianjin 300072China

Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.

关键词： Petroleum-contaminated soil Dagang Oilfield Bacterial community diversity 16s rRNA Illumina sequencing Environmental factor correlation analysis

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Research of 1,3-Dihydroxyacetone Production by Overexpressing Glycerol Transporter and Glycerol Dehydrogenase

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Transactions of Tianjin University 2019年第5期25卷 549-558页

作者： Jinxiu Tan Xiaona Yang wenyu lu School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China Key Laboratory of System Bioengineering (Tianjin University) Ministry of EducationTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) SynBio Research PlatformTianjin 300072China

1,3-Dihydroxyacetone (DHA), a natural ketose, is widely used in the chemical, cosmetic, and pharmaceutical industries. The current method for DHA production is Gluconobacter oxydans ( G. oxydans ) fermentation, but the high concentration of glycerol in the fermentation broth inhibits cells growth. To overcome this obstacle, in this study, we overexpressed the glycerol transporter (GlpFp) by the use of promoters P tufB , P gmr , P glp1 , and P glp2 in G. oxydans 621H. The results show that the glycerol tolerances of strains overexpressing G lpF were all much better than that of the control strain. The glycerol dehydrogenase gene (G dh) was overexpressed by the promoters P tufB and P gdh , which increased the DHA titer by 12.7% compared with that of the control group. When G lpF and Gdh genes were co-overexpressed in G. oxydans 621H, the OD600 value of the engineered strains all increased, but the DHA titers decreased in di erent degrees, as compared with strains that overexpressed only G dh . This study provides a reference for future research on DHA production.

关键词： G. oxydans 621H 1,3-dihydroxyacetone Glycerol Glycerol transporter Glycerol dehydrogenase

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Fed-Batch Fermentation for Spinosad Production in an Improved Reactor

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Transactions of Tianjin University 2017年第6期23卷 530-537页

作者： Chunzhe lu Jing Yin Chuanbo Zhang wenyu lu School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China Key Laboratory of System Bioengineering Ministry of EducationTianjin 300072China SynBio Research Platform Collaborative Innovation Center of Chemical Science and EngineeringTianjin 300072China

As a kind of aerobic bacteria, Saccharopolyspora spinosa exhibits a high demand for oxygen. In the fermentation process, the methods of increasing ventilation and improving agitation speed are usually adopted to achieve higher values of dissolved oxygen. These methods decrease the efficiency of spinosad biosynthesis.In this study, an improved reactor was designed to solve these problems. The exhaust gas reflux device, impellers,and baffles were improved. Furthermore, we established the kinetic models for the cell growth, substrate consumption and spinosad generation in batch fermentation process. The simulation results were in good agreement with the experimental data. Spinosad production reached 583.86 mg/L after employing the suitable feeding strategy by fed-batch fermentation in the improved reactor, whereas it was only 157.01 mg/L before optimization. The method described can provide insight to strengthen spinosad production and can be extended to the culturing process of filamentous aerobic bacteria.

关键词： Spinosad Bioreactors Fed-batch fermentation Saccharopolyspora spinosa

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