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Journal of Integrative Plant Biology 2024年第6期66卷 1106-1125页

作者： Wei Wang Jinyao Ouyang Yating Li Changsheng Zhai Bing He Huahan Si Kunsong Chen Jocelyn K.C.Rose wensuo jia College of Horticulture China Agricultural UniversityBeijing 100193China College of Agriculture and Biotechnology Zhejiang UniversityZijingang CampusHangzhou 310058China Plant Biology Section School of Integrative Plant ScienceCornell UniversityIthaca14853NYUSA Institute of Horticulture Crops Xinjiang Academy of Agricultural SciencesUrumqi 830000China

It is generally accepted that jasmonate-ZIM domain(JAZ)repressors act to mediate jasmonate(JA)signaling via CORONATINE-INSENSITIVE1(COI1)-mediated ***,we report a cryptic signaling cascade where a JAZ repressor,FvJAZ12,mediates multiple signaling inputs via phosphorylation-modulated subcellular translocation rather than the COI1-mediated degradation mechanism in strawberry(Fragaria vesca).FvJAZ12 acts to regulate flavor metabolism and defense response,and was found to be the target of Fv MPK6,a mitogen-activated protein kinase that is capable of responding to multiple signal ***6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues,thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2,which acts to control the expression of lipoxygenase 3(FvLOX3),an important gene involved in JA biosynthesis and a diverse array of cellular *** data reveal a previously unreported mechanism for JA signaling and decipher a signaling cascade that links multiple signaling inputs with fruit trait development.

关键词： defense response JAZ repressor phosphorylation strawberry subcellular translocation

Modulation of Root Signals in Relation to Stomatal Sensitivity to Root-sourced Abscisic Acid in Drought-affected Plants

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Journal of Integrative Plant Biology 2007年第10期49卷 1410-1420页

作者： Huibo Ren Kaifa Wei wensuo jia William John Davies jianhua Zhang College of Agronomy and Biotechnology State Key Laboratory of Plat Physiology and Biochemistry China Agricultural UniversityBeijing China100094 Department of Biological Sciences Lancaster Environment Centre University of Lancaster Lancaster LAl4YQ.UK Department of Biology Hong Kong Baptist University Kowloon Tong Hong Kong China

Stomatal sensitivity to root signals induced by soil drying may vary between environments and plant species. This is likely to be a result of the interactions and modulations ámong root signals. As a stress signal, abscisic acid （ABA） plays a central role in root to shoot signaling, pH and hydraulic signals may interact with ABA signals and thus, jointly regulate stomatal responses to changed soil water status, pH itself can be modified by several factors, among which the chemical compositions in the xylem stream and the live cells surrounding the vessels play crucial roles. In addition to the xylem pH, more attention should be paid to the direct modulation of leaf apoplastic pH, because many chemical compositions might strongly modify the leaf apoplastic pH while having no significant effect on the xylem pH. The direct modulation of the ABA signal intensity may be more important for the regulation of stomatal responses to soil drying than the ABA signal per se. The ABA signal is also regulated by the ABA catabolism and the supply of precursors to the roots if a sustained root to shoot communication of soil drying operates at the whole plant level. More importantly, ABA catabolism could play crucial roles in the determination of the fate of the ABA signal and thereby control the stomatal behavior of the root-sourced ABA signal.

关键词： abscisic acid drought pH root signal stomatal sensitivity

Localization of NOS-like protein in guard cells of Vicia faba *** its possible function

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Chinese Science Bulletin 2007年第1期52卷 84-90页

作者： LIU Xin WANG YouQun jia wensuo LOU ChengHou ZHANG ShuQiu State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beiiing 100094 China Department of Life Science Lai Yang Agricultural College Qingdao 266109 China

Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of ni- tric oxide synthase (NOS)-like proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide (NO) and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS in- hibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO genera- tion from NOS pathway is the main pathway in the stress and JA responses. The pharmacological ex- periment showed that increasing the Ca2+ at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca2+-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role in plant stress responses.

关键词：免疫细胞氧化一氮连位化合物生物化学

Roles of a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis

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Chinese Science Bulletin 2007年第4期52卷 484-491页

作者： REN HuiBo FAN Yijian GAO ZhiHui WEI KaiFa LI GuiFen LIU Jing CHEN Lin LI BingBing HU jianFang jia wensuo State Key Laboratory of Plant Biochemistry College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China

ABA, acting as a stress signal, plays crucial roles in plant resistance to water stress. Because ABA signal production is based on ABA biosynthesis, the regulation of NCED, a key enzyme in the ABA biosynthesis pathway, is normally thought of as the sole factor controlling ABA signal production. Here we demonstrate that ABA catabolism in combination with a synergistic regulation of ABA biosynthesis plays a crucial role in governing ABA signal production. Water stress induced a significant accumulation of ABA, which exhibited different patterns in detached and attached leaves. ABA catabolism followed a temporal trend of exponential decay for both basic and stress ABA, and there was little difference in the catabolic half-lives of basic ABA and stress ABA. Thus, the absolute rate of ABA catabolism, i.e. the amount of ABA catabolized per unit time, increases with increased ABA accumulation. From the dynamic processes of ABA biosynthesis and catabolism, it can be inferred that stress ABA accumulation may be governed by a synergistic regulation of all the steps in the ABA biosynthesis pathway. Moreover, to maintain an elevated level of stress ABA sustained activation of NCED3 should be required. This inference was supported by further findings that the genes encoding major enzymes in the ABA biosynthesis pathway, e.g. NCED3, AAO3 and ABA3 were all activated by water stress, and with ABA accumulation progressing, the expressions of NCED3, AAO3 and ABA3 remained activated. Data on ABA catabolism and gene expression jointly indicate that ABA signal production is controlled by a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism.

关键词：拟南芥 ABA信号 ABA生物合成分解代谢协同调节 NCED3 持续致活水胁迫干旱

CRISPR/Cas9-introduced single and multiple mutagenesis in strawberry

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Journal of Genetics and Genomics 2018年第12期45卷 685-687页

作者： Sinian Xing Meiru jia Lingzhi Wei Wenwen Mao Usman Ali Abbasi Yaoyao Zhao Yating Chen Minglin Cao Kai Zhang Zhengrong Dai Zhechao Dou wensuo jia Bingbing Li College of Horticulture China Agricultural University

CRISPR/Cas9-mediated genome editing is a powerful tool for life science research. Recently, strawberry (Fragaria × ananassa), an important horticultural crop, has emerged as a model organism for investigating the regulatory mechanisms of fruit development and ripening (Shulaev et al., 2011; jia et al., 2013, 2017; Kang et al., 2013; Han et al., 2015). While most cultivated strawberries

关键词： PDS CRISPR/Cas9-introduced single and multiple mutagenesis in strawberry

Drought tolerance evaluation of tobacco plants transformed with different set of genes under laboratory and field conditions

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Science Bulletin 2015年第6期60卷 616-628,M0003页

作者： Yuanhua Wang Ruihong Dang Jinxi Li Yu Han Ning Ding Xingliang Li Meiru jia Ziqiang Li Lingzhi Wei Jinzhu jiang Yijuan Fan Bingbing Li wensuo jia College of Agronomy and Biotechnology China AgriculturalUniversity Beijing 100193 China

Although extensive studies have demonstrated that many drought-responsive genes confer drought toler- ance to plants, comparisons of the drought tolerance capa- bilities conferred by different genes under various natural conditions have seldom been reported. We evaluated and compared the effects of two sets of transgenes, the drought- responsive genes （AtDREBIB and AtCBL1） and the root architecture-regulated genes （iaaM and AtCKX）, on drought tolerance in Nicotiana tabacum plants subjected to different conditions. The expression of AtCKX3 driven by a root- specific promoter PYKIO （designated hereafter as PIO; PIO：AtCKX3）, 35S：AtCKX3, or PlO：iaaM promoted root growth and development. Compared to plants harboring PIO：AtCKX3, 35S：AtCKX3, PlO：iaaM, or the empty vector, those carrying 35S：AtDREB1B, 35S：AtCBLl, or 35S：iaaM exhibited increased drought tolerance under laboratory- controlled conditions. Conversely, in field conditions, plants transformed with 35S：AtDREB1, 35S：AtCBL1, or 35S：iaaM were sensitive to drought stress. Under field conditions, drought stress dramatically reduced the growth and seed production of plants harboring 35S：AtDREBIB, 35S：A tCBL1, 35S：iaaM, or the empty vector, whereas it had little effect on plants carrying PIO：AtCKX3, 35S：AtCKX3, or PlO：iaaM. This study demonstrates that a plant＇s tolerance to drought stress changes with environmental conditions, and our results indicates that manipulating the expression of genes that control root architecture may be important for engineering plants with improved drought tolerance in natural conditions.

关键词： Drought tolerance Transgene Rootgrowth Environment Tobacco plants

Protein phosphorylation is involved in the water stress-induced ABA accumulation in the roots of Malus hupehensis Rehd

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Chinese Science Bulletin 2001年第10期46卷 855-858页

作者： ZHANG Dapeng, YANG Hongqiang, jia wensuo & HUANG Conglin1. Laboratory of Molecular Developmental Biology of Fruit Trees, China Agricultural University, Beijing 100094, China 2. The Key Laboratory of Plant Physiology and Biochemistry of Agricultural Ministry, China Agricultural University, Beijing 100094, China Laboratory of Molecular Developmental Biology of Fruit Trees China Agricultural University Beijing China The Key Laboratory of Plant Physiology and Biochemistry of Agricultural Ministry China Agricultural University Beijing China

Water stress-induced ABA accumulation plays a key role in the root to shoot communication and/or the cell to cell signaling under the soil stresses. The signaling of the water stress itself that leads to the accumulation, however, is less known. In this study, we subjected the roots of Malus hupehensis seedlings to water stress treatment and investigated the ABA accumulation in relation to protein phos-phorylation. Our results showed that ABA accumulation could be substantially triggered in 40 min and reached 4 folds in 100 min after treatment with 30% PEG 6000 (polyethylene glycol). The water stress treatment also led to a substantial enhancement of total kinase activity, assessed with histone-111 as substrate, in 15 min and a maximum enhancement in 30 min before it declined to initial level. The Ca2+-dependent kinase activity showed a similar, if not more sensitive, trend. When the roots were fed with labeled 32P-ATP, water stress enhanced the labeling of proteins, which showed a maximum labeling at

关键词： ABA (abscisic acid) water stress protein kinases Malus hupehensis Rehd.

An effector-reporter system to study cellular signal transduction in strawberry fruit (Fragaria ananassa)

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Horticulture Research 2021年第1期8卷 842-854页

作者： Baozhen Zeng Tianyu Li Wei Wang Zhengrong Dai Jie Li Zhiyuan Xi Kenan jia Yu Xing Bingbing Li jiaqi Yan wensuo jia College of Horticulture China Agricultural UniversityBeijing 100193China College of International Education Beijing University of Chemical TechnologyBeijing 100029China College of Plant Science and Technology Beijing University of AgricultureBeijing 102206China

An effector-reporter system is a powerful tool used to study cellular signal transduction,but this technique has been traditionally used in protoplasts.A similar system to study cellular signal transduction in fruits has not yet been *** this study,we aimed to establish an effector–reporter system for strawberry fruit,a model nonclimacteric *** first investigated the characteristics of transient gene expression in strawberry fruits and found marked variation in gene expression levels among individual fruits,and this variation has complicated the establishment of a technical *** overcome this difficulty,we investigated a sampling strategy based on a statistical analysis of the activity pattern of four different reporters(GUS,GFP,FLuc,and RLuc)among individual fruits and combinations of pairs of reporters(GUS/GFP and RLuc/FLuc).Based on an optimized sampling strategy,we finally established a step-by step protocol for the effector/reporter *** FaMYB10 and FaWRKY71 as the effectors and GUS driven by the FaCHS promoter as the reporter,we demonstrated that this effector/reporter system was practical and *** effector/reporter technique will contribute to an in-depth exploration of the signaling mechanism for the regulation of strawberry fruit ripening.

关键词： straw Fragaria system

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葡萄种子细胞中脱落酸的胶体金免疫电镜定位

中国农业大学学报 1999年第5期4卷 111-117页

作者：黄丛林张大鹏贾文锁中国农业大学园艺学院中国农业大学

用胶体金免疫电镜技术，对脱落酸（ABA）在葡萄种子细胞超微结构水平上的分市进行了研究。按葡萄种子细胞液泡中是否含有电子密度大的染色物质，可将其分为含酚和无酚2个主要类型。在含酚细胞中电子密度大的染色物质呈多种状态存在，先... 详细信息

用胶体金免疫电镜技术，对脱落酸（ABA）在葡萄种子细胞超微结构水平上的分市进行了研究。按葡萄种子细胞液泡中是否含有电子密度大的染色物质，可将其分为含酚和无酚2个主要类型。在含酚细胞中电子密度大的染色物质呈多种状态存在，先是均匀而稀疏地分布在液池中，然后逐渐凝聚成块状，最后浓缩成一薄层并均匀分布在液泡膜内侧。无酚细胞中金颗粒主要标记在细胞核和细胞质，特别是细胞核有大量金颗粒标记，细胞壁有少量金颗粒标记，液泡中没有发现金颗粒标记。和无酷细胞相似，在含酚细胞中金颗粒主要标记在细胞核和细胞质，特别是细胞核金颗粒密度很大，细胞壁有极少金颗粒标记。引人注目的是液泡中聚集态的染色物质上也发现金颗粒标记，但稀疏而均匀分布的染色物质上几乎找不到金颗粒标记，当染色物质最终浓缩成薄层状态时则发现有大量的金颗粒标记。无论用免疫前兑血清染色的对照切片还是材料，不被EDC固定的对照切片中都很难找到金颗粒标记，说明该ABA免疫胶体金电镜定位结果是特异、可靠的。

关键词：葡萄种子脱落酸胶体金免疫电镜定位

Cell biological mechanism for triggering of ABA accumulation under water stress in Vicia faba leaves

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Science China(Life Sciences) 2001年第4期44卷 421-428页

作者：张大鹏何芳莲贾文锁 1. Laboratory of Molecular Developmental Biology of Fruit Trees Laboratory of Plant Physiology and Biochemistry China Agricultural University 100094 Beijing China

Water stress-induced ABA accumulation is a cellular signaling process from water stress perception to activation of genes encoding key enzymes of ABA biosynthesis, of which the water stress-signal perception by cells or triggering mechanism of the ABA accumulation is the center in the whole process of ABA related-stress signaling in plants. The cell biological mechanism for triggering of ABA accumulation under water stress was studied in leaves of Vicia faba. Mannitol at 890 mmol· kg-1 osmotic concentration induced an increase of more than 5 times in ABA concentra-tion in detached leaf tissues, but the same concentration of mannitol only induced an increase of less than 40 % in ABA concentration in protoplasts. Like in detached leaf tissues, ABA concentra-tion in isolated cells increased more than 10 times under the treatment of mannitol at 890 mmol·kg-1 concentration, suggesting that the interaction between plasmalemma and cell wall was essential to triggering of the water stress-induced ABA accumulation. Neither Ca2+-che- lating agent EGTA nor Ca2+ channel activator A23187 nor the two cytoskeleton inhibitors, colchicine and cyto-chalasin B, had any effect on water stress-induced ABA accumulation. Interestingly water stress-induced ABA accumulation was effectively inhibited by a non-plasmalemma-perme- able sulfhy-dryl-modifier PCMBS (p-chloromercuriphenyl-sulfonic acid), suggesting that plasmalemma pro-tein(s) may be involved in the triggering of water stress-induced ABA accumulation, and the protein may contain sulfhydryl group at its function domain.

关键词： water stress, ABA (abscisic acid), PCMBS (p-chloromercuriphenyl-sulfonic acid), cytoskeleton, Ca2+, Vicia faba.