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Structure of the catalytic domain of a state transition kinase homolog from Micromonas algae

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Protein & Cell 2013年第8期4卷 607-619页

作者： Jiangtao Guo Xuepeng Wei Mei Li Xiaowei Pan wenrui chang Zhenfeng Liu National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100039China

Under natural environments,plants and algae have evolved various photosynthetic acclimation mechanisms in response to the constantly changing light *** state transition and long-term response processes in photosynthetic acclimation involve remodeling and composition alteration of thylakoid membrane.A chloroplast protein kinase named Stt7/STN7 has been found to have pivotal roles in both state transition and longterm *** we report the crystal structures of the kinase domain of a putative Stt7/STN7 homolog from Micromonas ***299(MsStt7d)in the apo form and in complex with various nucleotide ***7d adopts a canonical protein kinase fold and contains all the essential residues at the active site.A novel hairpin motif,found to be a conserved feature of the Stt7/STN7 family and indispensable for the kinase stability,interacts with the activation loop and fi xes it in an active *** have also demonstrated that MsStt7d is a dualspecifi city kinase that phosphorylates both Thr and Tyr ***,preliminary in vitro data suggest that it might be capable of phosphorylating a consensus N-terminal pentapeptide of light-harvesting proteins Micromonas Lhcp4 and Arabidopsis Lhcb1 *** potential peptide/protein substrate binding site is predicted based on the location of a pseudo-substrate contributed by the adjacent molecule within the crystallographic *** structural and biochemical data presented here provide a framework for an improved understanding on the role of Stt7/STN7 in photosynthetic acclimation.

关键词： Stt7/STN7 kinase state transition phosphorylation

Crystal Structure of a Multilayer Packed Major Light-Harvesting Complex： Implications for Grana Stacking in Higher Plants

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Molecular Plant 2014年第5期7卷 916-919页

作者： Tao Wan Mei Li Xuelin Zhao Jiping Zhang Zhenfeng Liu wenrui chang National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences 15th Datun Road Chaoyang District Beijing 100101 People's Republic of China University of Chinese Academy of Sciences 19A Yuquan Road Beijing 100049 People's Republic of China

Dear Editor, Grana thylakoids in plants comprise multiple, tightly appressed thylakoid membranes in the chloroplast, which greatly increase the area-to-volume ratio and significantly improve the ability of chloroplasts to capture light. Grana layers are stacked and interact through the stromal surface of proteins embedded in the grana membranes,

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Structural insights into the catalytic mechanism of aldehyde-deformylating oxygenases

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Protein & Cell 2015年第1期6卷 55-67页

作者： Cheniun Jia Mei Li Jianjun Li Jingjing Zhang Hongmei Zhang Peng Cao Xiaowei Pan Xuefeng LU wenrui chang National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Key Laboratory ofBiofuels Shandong Provincial Key Laboratory of Energy Genetics Qingdao Instituie of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao 266101 China University of Chinese Academy of Sciences Beijing 100049 China

The fatty alk（a/e）ne biosynthesis pathway found in cyanobacteria gained tremendous attention in recent years as a promising alternative approach for biofuel production. Cyanobacterial aldehyde-deformylating oxygenase （cADO）, which catalyzes the conversion of Cn fatty aldehyde to its corresponding Cn-1 alk（ale）ne, is a key enzyme in that pathway. Due to its low activity, alk（a/e）ne production by cADO is an inefficient process. Previous biochemical and structural investi. gations of cADO have provided some information on its catalytic reaction. However, the details of its cata- lytic processes remain unclear. Here we report five crystal structures of cADO from the Synechococcus elongates strain PCC7942 in both its iron-free and iron-bound forms, representing different states during its catalytic process. Structural comparisons and functional enzyme assays indicate that Giu144, one of the iron-coordinating residues, plays a vital role in the catalytic reaction of cADO. Moreover, the helix where Glu144 resides exhibits two distinct conformations that correlates with the different binding states of the di-iron center in cADO structures. Therefore, our results provide a structural explanation for the highly labile feature of cADO di-iron center, which we pro- posed to be related to its low enzymatic activity. On the basis of our structural and biochemical data, a possible catalytic process of cADO was proposed, which could aid the design of cADO with improved activity.

关键词： aldehyde-deformylating oxygenase di-ironcenter crystal structure catalytic mechanism alk(a/e)neproduction

Structural implications of Dpy30 oligomerization for MLL/SET1 COMPASS H3K4 trimethylation

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Protein & Cell 2015年第2期6卷 147-151页

作者： Hongmei Zhang Mei Li Yu Gao Chenjun Jia Xiaowei Pan Peng Cao Xuelin zhao Jiping Zhang wenrui chang Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

Dear Editor, The methylation modifications of histone 3 lysine 4 （H3K4） have essential effects on biological processes including gene expression and transcription, cell cycle progression, and DNA repair. From yeast to mammals, the SET1 and MLL-like （mixed-lineage leukemia） multi-subunit protein complexes, known as SET1 or MLL COMPASS （Schneider et al., 2005）, are responsible for H3K4 methylation. In addition to the catalytic SET domain, the SET1 and MLL COMPASS complexes also contain a number of conserved subunits including WDR5 （Cps30）, RbBP5 （Cps50）.

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Crystal Structure Analysis of Extrinsic PsbP Protein of Photosystem II Reveals a Manganese- Induced Conformational change

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Molecular Plant 2015年第4期8卷 664-666页

作者： Peng Cao Yuan Xie Mei Li Xiaowei Pan Hongmei Zhang Xuelin Zhao Xiaodong Su Tao Cheng wenrui chang National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences 15th Datun Road Chaoyang District Beijing 100101 China University of Chinese Academy of Sciences 19A Yuquan Road Beijing 100049 China

The Mn cluster （Mn4CaO5） on the thylakoid luminal side of photosystem II （PSII） catalyzes the photosynthetic oxygen-evolving reaction, an essential process for life on Earth. In higher plants and green algae, the Mn cluster is surrounded by the membraneextrinsic proteins PsbO （33 kDa）, PsbP （23 kDa）, and PsbQ （17 kDa）（Murata and Miyao, 1985）.

关键词： conformational identified oleracea

REFINED CRYSTAL STRUCTURE OF INSULIN AT 1.8A RESOLUTION——HYDROGEN BONDS AND BOUND WATER

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Science in China,Ser.B 1982年第7期25卷 711-724页

作者：王大成常文瑞戴金壁 Institute of Biophysics Academia Sinica Beijing

The hydrogen bonds in insulin fall into three cases: the helical hydrogen bonds in α- or 310helices, the non-helical one formed by polar groups of insulin itself, and the hydrogen bondsformed between insulin and water. By using the information obtained, the results of a seriesof biochemical investigations on insulin analogs related to B-chain C-terminal peptide can beinterpreted and it can also be inferred that the complex behaviours of the aggregation ofinsulin may play a protective role for the unique conformation of the molecule. Water structure also appears in the refined model. About one third of the water in anasymmetric unit is hydrogen-bonded to insulin molecules or each other, which are referred toas bound water. The polar and charged groups of insulin all show the tendencies to bind towater molecules as many as possible, which is a significant factor for the stabilization of theunique conformation of the molecule. The binding way of water molecules to insulin mole-cules is also analysed.

关键词： NHB HYDROGEN BONDS AND BOUND WATER REFINED CRYSTAL STRUCTURE OF INSULIN AT 1.8A RESOLUTION

REFINED CRYSTAL STRUCTURE OF INSULIN AT 1.8A RESOLUTION——CRYST

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Science in China,Ser.B 1982年第6期25卷 603-610页

作者：王大成常文瑞戴金壁 Institute of Biophysics Academia Sinica Academia Sinica Beijing

The structure of insulin has been refined by difference Fourier method at 1.8A resolu-tion. A set of computer programs calculating the mF_o-nF_c Fourier synthesis and the cor-rections of parameters automatically has been set up. With the programs to refine theinsulin model obtained from MIR map at 1.8A resolution for 11 cycles, the R index(R=∑|KF_o- F_c|/∑KF_o) is reduced to 0.210 from the initial value of 0.388. Duringthe refinement the stereochemistry of the insulin molecules is constantly detected andadjusted to fit the reasonable geometry. The refinement has greatly improved the structuremodel of insulin and provided more detailed structure information. On the basis of this thesystems of hydrogen bond in an insulin dimer are determined and the interaction betweenwater and insulin molecules in the crystal is investigated.

关键词： REFINED CRYSTAL STRUCTURE OF INSULIN AT 1.8A RESOLUTION CRYST

Crystal Structure of 2-Haloacid Dehalogenase from Pseudomonas syringae pv. tomato DC3000

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Crystal Structure of 2-Haloacid Dehalogenase from Pseudomona...

Photoluminescence properties of Ca-doped BaMg Al_(10)O_(17):Eu^(2+),Mn^(2+) blue phosphor using BaF_2 and CaF_2 as co-flux

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作者： Zhiqiang Hou Hongmei Zhang Mei Li wenrui chang National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Graduate University of the Chinese Academy of Sciences

2-Haloacid dehalogenases（2-HADs） catalyse the hydrolylic dehalogenation of 2-haloalkanoic acid, cleaving the carbon-halide bond at the Cα atom position and releasing a halogen atom. These enzymes are of interest for their potential use in bioremediation and the synthesis of industrial chemicals. Here we report the crystal structure of 2-HAD from Pseudomonas syringae pv. tomato DC3000（ps-2-HAD） at 1.98 A resolution solved by the single wavelength anomalous dispersion method. A ps-2-HAD molecule consists of two structurally distinct domains: the core domain and the sub domain. Enzymatic activity analysis of ps-2-HAD revealed its capacity to catalyse both L- and D- substrates;however, the structure of ps-2-HAD is completely different to DehI, the only structurally characterised DL-2-HAD enzyme, but shows similar overall folding to L-HADs. Single mutations of four amino acid residues at the putative active site showed that they are related to its enzymatic activity, yet three of them are unconserved among the HADs. These observations imply that ps-2-HAD has a novel active site and a unique catalytic behaviour compared to other HADs. This study provides a structural basis and biochemical evidence for further elucidation of the catalytic mechanism of 2-HAD.

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Journal of Rare Earths 2014年第12期32卷 1109-1113页

作者：冶林琴朋兴平张水合王育华常文瑞 Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education Lanzhou University Department of Materials Science School of Physical Science and Technology Lanzhou University

Ca-doped Ba Mg Al10O17：Eu2＋,Mn2＋（BAM） blue phosphors were synthesized by flux assisted solid-state reaction method using Ca F2 and Ba F2 as *** dispersity and particle size homogenization of hexagonal pure phase BAM were obtained by sintering at 1400 ℃.The effects of the Ca2＋ ions content on the structure, morphology and photoluminescence properties of the phosphors were *** results indicated that the incorporation of Ca could decrease the lattice constant, improve the homogeneity and dispersity and enhance the photoluminescence（PL） intensity of the phosphor *** optimum Ba0.86Ca0.04Mg0.97Al10O17：0.1Eu2＋,0.03Mn2＋ PL intensity was enhanced for about 30% and relative brightness was improved about 4%.Furthermore, the synthesized BAM and commercial BAM phosphors were annealed for 30 min at 600 oC in *** Ca-doped phosphors had stronger emission intensity, higher brightness and better chromaticity stability than that of the commercial *** results indicated that Ca-doped blue phosphors had good potential applications in the commercial tricolor fluorescent lamps as well as in other display and lamps.

关键词： BaMgAl10O17:Eu2+ Mn2+ BAM CaF2 flux phosphor rare earths