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The outpost against cancer:universal cancer only markers

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Cancer Biology & Medicine 2023年第11期20卷 806-815页

作者： Chengchen Qian Xiaolong Zou Wei Li Yinshan Li wenqiang yu Shanghai Epiprobe Biotechnology Co. LtdShanghai 200233China Department of General Surgery the First Affiliated Hospital of Harbin Medical UniversityHarbin 150001China Shandong Epiprobe Medical Laboratory Co. LtdHeze 274108China People's Hospital of Ningxia Hui Autonomous Region Ningxia Medical UniversityYinchuan 750002China Shanghai Public Health Clinical Center&Department of General Surgery Huashan Hospital&Cancer Metastasis Institute&Laboratory of RNA EpigeneticsInstitutes of Biomedical SciencesShanghai Medical CollegeFudan UniversityShanghai 200032China

Cancer is the leading cause of death worldwide.Early detection of cancer can lower the mortality of all types of cancer;however,effective early-detection biomarkers are lacking for most types of cancers.DNA methylation has always been a major target of interest because DNA methylation usually occurs before other detectable genetic changes.While investigating the common features of cancer using a novel guide positioning sequencing for DNA methylation,a series of universal cancer only markers(UCOMs)have emerged as strong candidates for effective and accurate early detection of cancer.While the clinical value of current cancer biomarkers is diminished by low sensitivity and/or low specificity,the unique characteristics of UCOMs ensure clinically meaningful results.Validation of the clinical potential of UCOMs in lung,cervical,endometrial,and urothelial cancers further supports the application of UCOMs in multiple cancer types and various clinical scenarios.In fact,the applications of UCOMs are currently under active investigation with further evaluation in the early detection of cancer,auxiliary diagnosis,treatment efficacy,and recurrence monitoring.The molecular mechanisms by which UCOMs detect cancers are the next important topics to be investigated.The application of UCOMs in real-world scenarios also requires implementation and refinement.

关键词： Cancer detection cancer screening DNA methylation cancer epigenetics cancer biomarkers

Steering Against Wind： A New Network of NamiRNAs and Enhancers

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Genomics, Proteomics & Bioinformatics 2017年第5期15卷 331-337页

作者： ying liang qingping zou wenqiang yu Laboratory of RNA Epigenetics.Institutes of Biomedical Sciences&Department of General Surgery Huashan Hospital Cancer M etastasis Institute Fudan UniversityShanghai 200032China Department of Biochemistry and Molecular Biology Shanghai Medical College MOE Key Laboratory of Metabolism and Molecular MedicineDepartment of Molecular BiologyFudan UniversityShanghai 200032China Collaborative Innovation Center for Genetics and Development School of Life SciencesFudan UniversityShanghai 200433China

MicroRNAs （miRNAs） are a class of endogenous non-coding RNAs with regulatory functions. Traditionally, miRNAs are thought to play a negative regulatory role in the cytoplasm by binding to the yuTR of target genes to degrade mRNA or inhibit translation. However, it remains a challenge to interpret the potential function of many miRNAs located in the nucleus. Recently, we reported a new type of miRNAs present in the nucleus, which can activate gene expres- sion by binding to the enhancer, and named them nuclear activating miRNAs （NamiRNAs）. The discovery of NamiRNAs showcases a complementary regulatory mechanism of miRNA, demon- strating their differential roles in the nucleus and cytoplasm. Here, we reviewed miRNAs in nucleus to better understand the function of NamiRNAs in their interactions with the enhancers. Accord- ingly, we propose a NamiRNA--enhancer-target gene activation network model to better under- stand the crosstalk between NamiRNAs and enhancers in regulating gene transcription. Moreover, we hypothesize that NamiRNAs may be involved in cell identity or cell fate determina- tion during development, although further study is needed to elucidate the underlying mechanisms in detail.

关键词： Nuclear activating miRNAs Tissue-specific enhancers Transcriptional gene activa-tion Cell identity Cell fate

Mutually exclusive epigenetic modification on SIX6 with hypermethylation for precancerous stage and metastasis emergence tracing

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2683-2686页

作者： Shihua Dong Zhicong Yang Peng Xu Wanxiang Zheng Baolong Zhang Fangqiu Fu Zhanrui Mao Jianlin yuan Haiquan Chen wenqiang yu Shanghai Epiprobe Biotechnology Co. LtdShanghaiChina Shanghai Public Health Clinical Center&Department of General Surgery Huashan Hospital&Cancer Metastasis Institute&Institutes of Biomedical SciencesShanghai Medical CollegeFudan UniversityShanghaiChina Department of Urology Xijing HospitalAir Force Medical UniversityXi’anShaanxiChina Department of Thoracic Surgery Fudan University Shanghai Cancer Center&Institute of Thoracic Oncology&State Key Laboratory of Genetic EngineeringFudan UniversityShanghaiChina

Dear Editor,Aberrant DNA methylation gets involved in cancer initiation,progression,and recurrence,which in turn makes it an ideal cancer biomarker.Various methylation markers or their panels have been developed in diverse cancer types.However,the model-constructing based marker mining strategy and incompatibility of application have greatly impeded their ways to clinic.Thus,single methylation marker applicable to all/most cancer types and multiple clinical scenarios is desperately needed.The hope came from the unexpected observation that HIST1H4F was universally hypermethylated in all 17 cancer types;thus,we raised the concept of“Universal Cancer Only Marker(UCOM)”and established a paradigm for discovery and clinical application of UCOM.1 Recently,a novel UCOM,hypermethylated PCDHGB7,was identified and found to advance cervical cancer(CC)screening to the precancerous stage.2 During the screening of UCOM,we discerned a bunch of cancer cell-differentially methylated regions.1 Among them,sine oculis(SIX)homeobox family of transcription factors,which were found to function as tumorigenesis regulator by promoting epithelial-to-mesenchymal transition and metastasis recently in addition to their traditional roles in tissue formation and organogenesis,3 sparked our special attention.Herein,we interrogate whether SIX6 methylation could serve as a novel UCOM and its potential applications.

关键词： metastasis precancerous raised

四川盆地中部中侏罗统沙溪庙组沉积期古环境与古气候演化——以永浅1井为例

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地质学报 2024年第4期98卷 1211-1228页

作者：余文强杨田蔡来星李晓芳何青油气藏地质及开发工程全国重点实验室(成都理工大学) 沉积地质研究院四川成都610059

四川盆地中部中侏罗统沙溪庙组沉积期古环境与古气候研究对揭示该时期盆内的沉积格局、重建古地理及油气形成与演化具有重要的理论和实践意义。本文以四川盆地中部永浅1井的47件泥岩样品为研究对象,通过主微量元素的变化特征恢复研究区... 详细信息

四川盆地中部中侏罗统沙溪庙组沉积期古环境与古气候研究对揭示该时期盆内的沉积格局、重建古地理及油气形成与演化具有重要的理论和实践意义。本文以四川盆地中部永浅1井的47件泥岩样品为研究对象,通过主微量元素的变化特征恢复研究区沙溪庙组沉积期的古环境与古气候演化过程。研究结果表明,Sr/Ba比值、Rb/K_(2)O比值、Ca/Mg比值和Ca/(Ca+Fe)比值指示川中地区中侏罗统沙溪庙组沉积期整体为淡水环境,但在沙溪庙组一段沉积中、晚期出现了短暂的水体盐度升高;V/(V+Ni)比值、U/Th值、V/Cr比值与Ni/Co比值指示沙溪庙组沉积期整体为间歇暴露的浅水氧化环境,局部为弱氧化或还原环境;C值、CIA值和Rb/Sr比值等指标显示,沙溪庙组沉积期古气候总体为温暖湿润的气候环境,但期间发生波动性干旱,在温暖湿润和半干旱—半湿润之间频繁波动。多元地球化学参数为川中地区中侏罗统沙溪庙组沉积期的古气候演化和区域性古气候对比提供了新的证据。

关键词：川中地区中侏罗世中晚期沙溪庙组古环境古气候

基于混沌理论、变分模态分解和长短期记忆网络的地磁变化预测方法

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地震学报 2024年第1期46卷 92-105页

作者：于文强李厚朴刘敏宋立忠海军工程大学电气工程学院武汉430079 91001部队北京100081

针对地磁变化场的非平稳性、非线性以及物理模型难以预测的特点,提出了一种改进的长短期记忆网络预测方法并进行了验证。首先应用变分模态分解方法对地磁台站数据进行去噪,再根据地磁变化的混沌特性引入混沌理论对样本集进行优化,最终... 详细信息

针对地磁变化场的非平稳性、非线性以及物理模型难以预测的特点,提出了一种改进的长短期记忆网络预测方法并进行了验证。首先应用变分模态分解方法对地磁台站数据进行去噪,再根据地磁变化的混沌特性引入混沌理论对样本集进行优化,最终以长短期记忆网络预测地磁变化并对改进前后的方法进行了对比。结果显示,优化方法的预测效果较稳定,平均绝对误差小于2 nT,相关指数R~2超过0.8,预测值与实测值的拟合度较高,有效预测时长可达2.5天,且在中国大陆的泛用性较好。

关键词：变分模态分解混沌理论长短期记忆地磁变化

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3D打印颈椎枕的压力疼痛评估及信度分析

中国康复医学杂志 2024年第4期39卷 522-528页

作者：任富超姚远于文强马振江鲁德志王金武潍坊医学院康复医学院山东省潍坊市261053 上海交通大学医学院附属第九人民医院上海大学医学院

目的:通过信度分析压痛测量仪评估颈肩部压痛的可靠性,并为3D打印个性化颈椎枕的临床效果评估提供新方式。方法:选取健康受试者36例,分别由2位评估者使用压痛测量仪对其颈肩部肌肉的7个测量点进行2次压痛阈测量,间隔7天后再次测量,采用S... 详细信息

目的:通过信度分析压痛测量仪评估颈肩部压痛的可靠性,并为3D打印个性化颈椎枕的临床效果评估提供新方式。方法:选取健康受试者36例,分别由2位评估者使用压痛测量仪对其颈肩部肌肉的7个测量点进行2次压痛阈测量,间隔7天后再次测量,采用Spearson相关系数计算评估者内和评估者间的信度,并使用Mann-Whitney U检验比较男性与女性的压痛阈值(pressure pain thresholds,PPT)。个性化颈椎枕使用前后,使用压痛测量仪对13例颈椎病患者选取具有代表性的4个测量点进行PPT测量,用以评估3D打印颈椎枕的治疗效果。结果:除了受测点C5、C6的棘突间旁1cm右侧和肩胛上角左侧外,各受测点评估者内信度均>0.65,各受测点评估者间信度均>0.75,可信度高;C5、C6的棘突间旁1cm右侧和肩胛上角左侧的评估者内组内相关系数(intraclass correlation coefficient,ICC)分别为0.65和0.56,中等信度(P<0.01);健康男性的斜方肌右侧、肩胛上角R、C5、C6的棘突间旁1cm左侧平均值要大于女性(P<0.05);斜方肌右侧、C5、C6的棘突间旁1cm右侧和C5、6的棘突间旁1cm左侧受测点3D打印颈椎枕治疗后的PPT平均值比治疗前大,差异具有显著性意义(P<0.05),说明疼痛有改善。结论:压痛测量仪评估正常健康人颈部压痛阈值信度较高,该方法稳定可靠,可作为3D打印颈椎枕临床效果评估的有效工具。

关键词：压痛阈值测痛仪信度 3D打印颈椎枕

Hymecromone:a clinical prescription hyaluronan inhibitor for efficiently blocking COVID-19 progression

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Signal Transduction and Targeted Therapy 2022年第4期7卷 1287-1296页

作者： Shuai Yang yun Ling Fang Zhao Wei Li Zhigang Song Lu Wang Qiuting Li Mengxing Liu Ying Tong Lu Chen Daoping Ru Tongsheng Zhang Kaicheng Zhou Baolong Zhang Peng Xu Zhicong Yang Wenxuan Li yuanlin Song Jianqing Xu Tongyu Zhu Fei Shan wenqiang yu Hongzhou Lu Laboratory of RNA Epigenetics Institutes of Biomedical Sciences&Shanghai Public Health Clinical Center&Department of General SurgeryHuashan HospitalCancer Metastasis Institute Shanghai Medical CollegeFudan UniversityShanghaiP.R.China Shanghai Public Health Clinical Center Fudan UniversityShanghaiP.R.China Department of Radiology Shanghai Public Health Clinical CenterFudan UniversityShanghaiP.R.China Zhongshan Hospital Fudan UniversityShanghaiP.R.China The Third People’s Hospital of Shenzhen ShenzhenP.R.China

Currently,there is no effective drugs for treating clinically COVID-19 except dexamethasone.We previously revealed that human identical sequences of SARS-CoV-2 promote the COVID-19 progression by upregulating hyaluronic acid(HA).As the inhibitor of HA synthesis,hymecromone is an approved prescription drug used for treating biliary spasm.Here,we aimed to investigate the relation between HA and COVID-19,and evaluate the therapeutic effects of hymecromone on COVID-19.Firstly,HA was closely relevant to clinical parameters,including lymphocytes(n=158;r=−0.50;P<0.0001),C-reactive protein(n=156;r=0.55;P<0.0001),D-dimer(n=154;r=0.38;P<0.0001),and fibrinogen(n=152;r=0.37;P<0.0001),as well as the mass(n=78;r=0.43;P<0.0001)and volume(n=78;r=0.41;P=0.0002)of ground-glass opacity,the mass(n=78;r=0.48;P<0.0001)and volume(n=78;r=0.47;P<0.0001)of consolidation in patient with low level of hyaluronan(HA<48.43 ng/mL).Furthermore,hyaluronan could directly cause mouse pulmonary lesions.Besides,hymecromone remarkably reduced HA via downregulating HAS2/HAS3 expression.Moreover,89%patients with hymecromone treatment had pulmonary lesion absorption while only 42%patients in control group had pulmonary lesion absorption(P<0.0001).In addition,lymphocytes recovered more quickly in hymecromone-treated patients(n=8)than control group(n=5)(P<0.05).These findings suggest that hymecromone is a promising drug for COVID-19 and deserves our further efforts to determine its effect in a larger cohort.

关键词： prescription clinical identical

基于POA的师生合作评价在大学英语写作教学中的实践探索

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创新创业理论研究与实践 2024年第9期7卷 171-176页

作者：于文强张静广西外国语学院欧美语言文化学院

师生合作评价是产出导向法创设的一种新型评价模式，它将评、教、学融合为一体，既解决了教师在写作评价中遇到的难题，同时又以学生为中心，以教师为主导，让学生参与评价。该文以英语专业二年级学生为研究对象，开展1学期的师生合作... 详细信息

师生合作评价是产出导向法创设的一种新型评价模式，它将评、教、学融合为一体，既解决了教师在写作评价中遇到的难题，同时又以学生为中心，以教师为主导，让学生参与评价。该文以英语专业二年级学生为研究对象，开展1学期的师生合作评价教学实践，以期探究师生合作评价的可行性、可操作性和有效性。研究表明：师生合作评价可以激发学生写作的兴趣和动力，提高学生写作水平和能力，缓解大规模班额评价不足问题。

关键词： POA 师生合作评价大学英语写作教学实践效果写作能力

Distributed Solving Linear Algebraic Equations with Switched Fractional Order Dynamics

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Journal of Systems Science & Complexity 2023年第2期36卷 613-631页

作者： yu wenqiang CHENG Songsong HE Shuping Anhui Engineering Laboratory of Human-Robot Integration System and Intelligent Equipment School of Electrical Engineering and AutomationAnhui UniversityHefei 230601China

This paper proposes a novel distributed optimization algorithm with fractional order dynamics to solve linear algebraic equations.Firstly,the authors proposed“Consensus+Projection”flow with fractional order dynamics,which has more design freedom and the potential to obtain a better convergent performance than that of conventional first order algorithms.Moreover,the authors prove that the proposed algorithm is convergent under certain iteration order and step-size.Furthermore,the authors develop iteration order switching scheme with initial condition design to improve the convergence performance of the proposed algorithm.Finally,the authors illustrate the effectiveness of the proposed method with several numerical examples.

关键词： Distributed optimization fractional order dynamics initial condition iteration order linear equations