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Pituitary transcriptome profile of liver cancer mice with different syndromes reveals the relevance of pituitary to the cancer and syndromes

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Journal of Traditional Chinese Medicine 2014年第6期34卷 691-698页

作者： Xiaomei Liu Zhaoqin fang Zhiqiang Pan wenli Lu Zhonghua Wu Chao Liang Yuanyuan Zhang Department of Experimental Traditional Chinese Medicine School of Basic Medical Science Shanghai University of Traditional Chinese Medicine Experiment Center of Science and Technology Shanghai University of Traditional Chinese Medicine

OBJECTIVE: To investigate the relevance of the pituitary to liver syndromes and cancer by studying the pituitary transcriptome profile in liver cancer mice with different ***: The quantitative four diagnosis and syndrome differentiation methods were used to screen normal control mice without syndromes(NC), liver cancer mice with poisonous pathogenic factors syndrome(PPFS), and Qi deficiency syndrome mice(QDS). An Affymetrix Gene Chip MouseExon 1.0 ST Array was performed to detect the gene expression of different groups. Gene clustering was applied to analyze the gene expression patterns of the PPFS and QDS groups compared with the NC group. The transcriptional networks analysis tool, Fun Net, was used to enrich the biological categories of differentially expressed genes in the PPFS and QDS ***: Biological categories of differentially expressed genes showed that excessive metabolism and extracellular matrix interaction, insufficient communication of cells(especially nerve cells), and the bidirectional regulation of genetic information processing were enriched in both syndromes. However, the degree of excessive metabolism in the PPFS group was higher than that in the QDS *** hyperfunction of cancer and infection, and the hypofunction of the nervous and endocrine systems were obvious in the QDS ***: The pituitary plays an important role in the development of liver cancer and syndromes. This study further studied the role of the pituitary in the combination of disease and syndromes.

关键词： Liver neoplasms Syndrome Pituitarygland Transcriptome

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高镁对体外培养原代神经干细胞分化命运的影响及其机制

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国际医药卫生导报 2016年第11期22卷 1515-1519页

作者：廖旺金聪丽李梅范胜诺方文丽郑雨秋刘军中山大学孙逸仙纪念医院神经科广州510120 广东医科大学附属医院神经科湛江524023

目的探索高镁对神经发生过程中神经干细胞分化命运的影响及其潜在机制。方法从C57／BL6胎鼠大脑中分离神经干细胞，通过添加硫酸镁（MgSO4）调节培养基镁离子浓度并诱导分化，第6天通过免疫细胞化学荧光染色观察成熟神经元标记物β-Ⅲtu... 详细信息

目的探索高镁对神经发生过程中神经干细胞分化命运的影响及其潜在机制。方法从C57／BL6胎鼠大脑中分离神经干细胞，通过添加硫酸镁（MgSO4）调节培养基镁离子浓度并诱导分化，第6天通过免疫细胞化学荧光染色观察成熟神经元标记物β-Ⅲtubulin（Tuj1）和胶质细胞标记物胶质原纤维酸性蛋白（GFAP）的阳性细胞比例，Westernblot检测ERK1／2表达，qPCR检测Tujl、GFAP的mRNA水平。结果高镁组（0．8mM和1．0mM）相比于对照组（0．6mM），Tuj1阳性细胞的比例增加，GFAP阳性细胞比例减少，Tuj1表达上调，GFAP表达下调，pERK／ERK上调，差异具有统计学意义（P〈0．05），高镁组添加ERK抑制剂PD0325901后，以上指标与对照组比较差异无统计学意义（P〉0．05）。结论高镁可促进神经干细胞向神经元分化，抑制其向神经胶质细胞分化，其作用可能与ERK1／2的激活有关。

关键词：镁离子神经干细胞神经发生分化命运细胞外信号调节激酶

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Inhibitory effect of procyanidin B2 and tannin acid on cholesterol esterase and their synergistic effect with orlistat

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Food Science and Human Wellness 2024年第1期13卷 360-369页

作者： Xiangxin Li Yijing Pu Haitao Jiang Wenxiao Jiao Wenjun Peng wenli Tian Weibo Jiang Xiaoming fang Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China College of Food Science and Nutritional Engineering China Agricultural UniversityBeijing 100083China College of Food Science and Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353China

This study was aimed to analyze the effect of procyanidin B2(PC)and tannin acid(TA)on the activities of cholesterol esterase(CEase)and the inhibitory mechanisms of enzymatic *** interaction mechanisms were investigated by enzymatic kinetics,multi-spectroscopy methods,thermodynamics analysis,molecular docking,and dynamic *** and TA could bind with CEase and inhibit the activity of enzyme in a mixed-competitive manner and non-competitive manner,which was verified by molecular docking simulations and dynamics ***,PC and TA showed the synergistic inhibition with ***,UVvis and the thermodynamic analysis revealed that the complexes were formed from CEase and inhibitors by noncovalent *** revealed by the circular dichroism results,both PC and TA decreased enzymatic activities by altering the conformations of *** inhibition of PC and TA on CEase might be one mechanism for its cholesterol-lowering effect.

关键词： Cholesterol esterase Polyphenols Interaction mechanisms Molecular dynamic simulation Synergistic effect

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Dual inhibition of glycolysis and oxidative phosphorylation by aptamer-based artificial enzyme for synergistic cancer therapy

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Nano Research 2022年第7期15卷 6278-6287页

作者： Xiao fang Meng Yuan Junduan Dai Qianying Lin Yuhong Lin wenli Wang Yifan Jiang Haihui Wang fang Zhao Junye Wu Shumeng Bai Chunhua Lu Huanghao Yang MOE Key Laboratory for Analytical Science of Food Safety and Biology Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food SafetyState Key Laboratory of Photocatalysis on Energy and EnvironmentCollege of ChemistryFuzhou UniversityFuzhou 350108China College of Biological Science and Engineering Fuzhou UniversityFuzhou 350108China

Dual inhibition of glycolysis and oxidative phosphorylation(OXPHOS)can break the metabolic plasticity of cancer cells to inhibit most energy supply and lead to effective cancer ***,the pharmacokinetic difference among drugs hinders these two inhibitions to realize a uniform temporal and spatial ***,we report an aptamer-based artificial enzyme for simultaneous dual inhibition of glycolysis and OXPHOS,which is constructed by arginine aptamer modified carbon-dots-doped graphitic carbon nitride(AptCCN).AptCCN can circularly capture intracellular arginine attribute to the specific binding ability of arginine aptamers to arginine,and further catalyze the oxidation of enriched arginine to nitric oxide(NO)under red light *** vitro and in vivo experiments showed that arginine depletion and NO stress could inhibit glycolysis and OXPHOS,leading to energy blockage and apoptosis of cancer *** presented aptamer-based artificial enzyme strategy provides a new path for cell pathway regulation and synergistic cancer therapy.

关键词： aptamers artificial enzymes photochemistry energy blockage cancer therapy

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Adaptive pandemic management strategies for construction sites:An agent-based modeling approach

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Frontiers of Engineering Management 2024年第2期11卷 288-310页

作者： Chengqian LI Qi fang Ke CHEN Zhikang BAO Zehao JIANG wenli LIU School of Civil Engineering Hunan UniversityChangsha 410082China School of Civil Engineering Central South UniversityChangsha 410004China School of Civil and Hydraulic Engineering Huazhong University of Science and TechnologyWuhan 430074China School of Energy GeoscienceInfrastructureand SocietyHeriot-Watt UniversityEdinburghUK

In the face of sudden pandemics,it becomes crucial for project managers to quickly adapt and make informed decisions that anticipate the consequences of their *** highlights the need for proactive management strategies to enhance epidemic response ***,current research mainly emphasizes the negative impacts of pandemics,often neglecting the development of adaptable management approaches for construction *** study aims to fill this research void by developing strategies tailored to managing pandemics at construction *** agent-based modeling,the study simulates the movement patterns of workers and the consequent spread of an epidemic under different risk scenarios and management *** results indicate that measures such as wearing masks,managing group activities,and enforcing entry controls can significantly reduce epidemic spread on construction sites,with entry controls showing the greatest effectiveness.

关键词： epidemic transmission agent-based modeling safety management management strategy

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Recent advances in(4þ3)cycloaddition of allenes

Green Synthesis and Catalysis

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Green Synthesis and Catalysis 2024年第4期5卷 270-276页

作者： Yanyan Que wenli Lei Yin fang Shuzhong He Yang Chen School of Pharmaceutical Sciences Guizhou Engineering Laboratory for Synthetic DrugsGuizhou UniversityGuiyan 550025China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100191China

Allenes are a class of unsaturated compounds containing a propadiene structural moiety,exhibiting essential physiological,pharmacological,and various ***(4þ3)cycloaddition reaction has become an effective method for synthesizing seven-membered rings,especially for *** method has been continuously improved and ***,we review the(4þ3)cycloaddition of allenes,focusing on the developed methodologies and the outlook in this field from 2013 to 2023.

关键词： Allenes (4þ3)Cycloaddition Asymmetric catalysis Seven-membered heterocycles

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神经妥乐平对LPS刺激后小胶质细胞炎症因子及Toll样受体表达的干预效应

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国际医药卫生导报 2018年第9期24卷 1311-1317页

作者：郑雨秋廖旺范胜诺方文丽熊鹦廖劭伟李怡刘军中山大学孙逸仙纪念医院神经科广州510120

目的探讨神经妥乐平（NTP）对脂多糖（LPS）刺激后BV-2小胶质细胞炎症反应的干预效应及其潜在机制.方法用CCK-8法检测细胞活性,实时荧光逆转录PCR测定IL-1β、iNOS、TLR2、TLR4、TLR6和MyD88基因的mRNA表达,蛋白免疫印迹法检测TLR4和My... 详细信息

目的探讨神经妥乐平（NTP）对脂多糖（LPS）刺激后BV-2小胶质细胞炎症反应的干预效应及其潜在机制.方法用CCK-8法检测细胞活性,实时荧光逆转录PCR测定IL-1β、iNOS、TLR2、TLR4、TLR6和MyD88基因的mRNA表达,蛋白免疫印迹法检测TLR4和MyD88的蛋白表达.结果-定浓度范围内的LPS（100,1000ng/ml）和NTP（0.001,0.01,0.1NU/ml）处理细胞活力影响不大,与细胞对照组相比,差异无统计学意义（P〉0.05）.LPS刺激12h后小胶质细胞炎症因子（IL-1β、iNOS）和Toll样受体（TLR2、TLR4、TLR6）及其下游基因MyD88的mRNA表达上调,TLR4和MyD88蛋白表达增加,与对照组相比,差异有统计学意义（P〈0.05）;而预先用NTP（0.1NU/ml）处理细胞12h可明显抑制IL-1β、iNOS、TLR2、TLR4、TLR6、MyD88基因的mRNA表TLR4、MyD88的蛋白表达,以上指标与LPS处理组（1000ng/ml）相比,差异有统计学意义（P〈0.05）.结论NTP可抑制LPS诱导的BV-2细胞的炎症因子表达上调,其作用可能与NTP抑制Toll样受体及其下游基因表达有关.

关键词：神经妥乐平 LPS 小胶质细胞炎症因子 Toll样受体

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老年冠心病患者麻醉胃镜安全性的评估

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胃肠病学和肝病学杂志 2004年第3期13卷 310-312页

作者：方文莉吴云林刘宝宏江伟骏朱晨宋剑慧上海市同仁医院内科上海200050 上海第二医科大学附属瑞金医院消化内科

目的研究异丙酚和芬太尼联合静脉麻醉运用于老年冠心病患者胃镜检查中的安全性。方法选择胃镜检查中自愿接受麻醉的老年冠心病患者24例为麻醉胃镜组(A组),另30例常规胃镜检查的老年冠心病患者为非麻醉胃镜对照组(B组),A组于镜检前静... 详细信息

目的研究异丙酚和芬太尼联合静脉麻醉运用于老年冠心病患者胃镜检查中的安全性。方法选择胃镜检查中自愿接受麻醉的老年冠心病患者24例为麻醉胃镜组(A组),另30例常规胃镜检查的老年冠心病患者为非麻醉胃镜对照组(B组),A组于镜检前静脉推注异丙酚和芬太尼,检测两组患者在检查前、检查中、检查后的心率、心律、血压、氧饱和度和心电图表现等。结果麻醉组的各项生理指标较对照组稳定,引起心脏意外的风险明显减少,安全可行。结论异丙酚和芬太尼联合静脉麻醉运用于老年冠心病患者是安全可行的,但应在有经验的麻醉医师实施和监控下及有抢救条件的胃镜室进行。

关键词：老年人冠心病麻醉胃镜心电图临床资料

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Effect of different drying methods on the amino acids,α-dicarbonyls and volatile compounds of rape bee pollen

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Food Science and Human Wellness 2024年第1期13卷 517-527页

作者： Yanxiang Bi Jiabao Ni Xiaofeng Xue Zidan Zhou wenli Tian Valérie Orsat Sha Yan Wenjun Peng Xiaoming fang State Key Laboratory of Resource Insects Institute of Apicultural ResearchChinese Academy of Agricultural SciencesBeijing 100093China College of Engineering China Agricultural UniversityBeijing 100083China Bioresource Engineering Dept. McGill UniversitySte-Anne-de-Bellevue QC H9X 3V9Canada

The significant demand for high quality food has motivated us to adopt appropriate processing methods to improve the food nutritional quality and *** this study,the effects of five drying methods,namely,pulsed vacuum drying(PVD),freeze drying(FD),infrared drying(IRD),hot-air drying(HAD)and sun drying(SD)on free amino acids(FAAs),α-dicarbonyl compounds(α-DCs)and volatile compounds(VOCs)in rape bee pollen(RBP)were *** results showed that FD significantly released the essential amino acids(EAAs)compared with fresh samples while SD caused the highest *** was the dominantα-DCs in RBP and the highest loss was observed after *** were the dominant volatiles of RBP and SD samples contained more new volatile substances(especially aldehydes)than the other four drying ***,FD and PVD would be potential methods to effectively reduce the quality deterioration of RBP in the drying process.

关键词： Drying Bee pollen Free amino acids α-Dicarbonyl compounds Volatile compounds

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Facile Self-templating Melting Route Preparation of Biomass-derived Hierarchical Porous Carbon for Advanced Supercapacitors

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Chemical Research in Chinese Universities 2018年第6期34卷 983-988页

作者： WANG Can WANG Dianyu ZHENG Shuang fang Xueqing ZHANG wenli TIAN Ye LIN Haibo LU Haiyan JIANG Lei College of Chemistry Jilin University Changchun 130012 P. R. China University of Chinese Academy of Sciences Beijing 100049 P. R. China School of Materials Science and Engineering Tianjin University Tianjin 300350 P. R. China Technical Institute of Physics and Chemistry Chinese Academy of Sciences Beijing 100190 P. R. China Guangdong Guanghua Sei-Tech Co. Ltd. Shantou 515061 P R. China School of Chemistry Beihang Uiversity Beijing 100190 P. R. China

Biomass-derived porous carbons show great potential as electrode materials for supercapacitors due to the environmental friendliness. However, most of the carbonaceous electrode materials suffer from low specific capacitance and rate capacity because of the poor porosity. Here, we reported a simple and effective approach to prepare micro/nano-hierarchical structured carbon materials derived from rice husk by NaOH-KOH molten salt co-activation. The as-prepared activated carbons exhibit high porosity and suitable pore size distributions for more electrolyte ion adsorption, which are all beneficial for achieving remarkable electrochemical performances, such as high specific capacitance（194.6 F/g）, excellent rate capability（retention of 85.9%） and outstanding cycling stability. Thus, the above biomass-derived carbon materials with high porosity and micro/nano structures obtained by co-activation method offered a new insight into novel electrode material for the use in energy storage systems with high energy density and excellent rate performance.

关键词： Porosity co-Activation Electrode material Electrolyte Supercapacitor

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