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Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917

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Synthetic and Systems Biotechnology 2024年第1期9卷 165-175页

作者： Siyan Zhou Linlin Zhao wenjie zuo Yilin Zheng Ping Zhang Yanan Sun Yang Wang Guocheng Du Zhen Kang The Science Center for Future Foods Jiangnan UniversityWuxi214122China The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China

The probiotic bacterium Escherichia coli Nissle 1917(EcN)holds significant promise for use in clinical and biological ***,the reliance on antibiotics to maintain plasmid-borne genes has overshadowed its *** this study,we addressed this issue by engineering the endogenous cryptic plasmids pMUT1 and *** non-essential elements were removed to create more stable derivatives pMUT1NR△and pMUT2HBC△.Synthetic promoters by integrating binding motifs on sigma factors were further constructed and applied for expression of Bacteroides thetaiotaomicron heparinaseⅢand the biosynthesis of *** to traditional antibiotic-dependent expression systems,our newly constructed antibiotic-free expression systems offer considerable advantages for clinical and synthetic biology applications.

关键词： Cryptic plasmids Antibiotic-free Gene expression Plasmid stability E.coli Nissle 1917

Characterization of the effects of hyaluronan oligosaccharides on human gut microbiota using a simulated batch fermentation system

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Food Science and Human Wellness 2024年

作者： Xinwei Gao Hongkang Zhu Litao Hu Jieyu Sun wenjie zuo Ping Liu Yang Wang Guocheng Du Zhen Kang The Science Center for Future Foods， Jiangnan University The Key Laboratory of Carbohydrate Chemistry and Biotechnology， Ministry of Education， Jiangnan University The Key Laboratory of Industrial Biotechnology， Ministry of Education， School of Biotechnology， Jiangnan University School of Food Science and Technology， Jiangnan University

The prebiotic effects of hyaluronan (HA) are widely recognized， contributing to improved gut health and immune modulation. Despite its extensive use as dietary supplement， the specific interactions between HA oligosaccharides (o-HAs) and the gut microbiome remain largely unexplored. To investigate its role and metabolic fate in gut homeostasis， 200 mg/day of o-HAs (average molecule weight 1k Da) were added to an automated computer-controlled SIMulator of the Gastrointestinal tract (SIMGI). The results revealed a significant reshaping of the intestinal flora composition by o-HAs， notably reducing the Firmicutes/Bacteroides ratio. Fermentation of o-HAs by gut microbiota significantly increased the abundance of Bifidobacteria， Prevotellaceae＿Prevotella， Dialister， Eubacterium， and Sutterella， but decreased that of Catenibacterium， Oscillospira， Klebsiella， and Citrobacter (P < 0.05). This corresponded with significant enhancements in the content of short-chain fatty acids (SCFAs) such as acetic acid， propionic acid and n-butyric acid， highlighting the significant impact of o-HAs at the genus level. Furthermore， analysis of microbial function predicted the downregulation of pathological events in nine human diseases， particularly infectious ones (parasitic and bacterial). Potential inhibitions were observed in metabolic pathways associated with pentose and glucuronate interconversions as well as cationic antimicrobial peptide resistance. These findings underscore the in vitro prebiotic effects of o-HAs and their potential relevance in managing diverticular diseases or preventing metabolic disorders through the regulation of gut microbiota.

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芽胞杆菌JZB30-1的鉴定及其对番茄灰霉病的生防作用

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植物保护 2024年第3期50卷 165-175,202页

作者：赵娟左文杰张殿朋秦文韬卢彩鸽北京市农林科学院植物保护研究所北京100097 农业农村部北方果蔬有害生物绿色防控重点实验室(部省共建) 北京100097 北京农学院生物与资源环境学院北京102206

为获得对番茄灰霉病具有良好防效的生防芽胞杆菌,采用对峙培养、双层平板以及生防相关特性检测等方法筛选高活性拮抗菌株,根据形态学、生理生化特性及多基因测序进行菌株种类鉴定,通过离体果实和盆栽试验明确供试菌株的防病促生效果。... 详细信息

为获得对番茄灰霉病具有良好防效的生防芽胞杆菌,采用对峙培养、双层平板以及生防相关特性检测等方法筛选高活性拮抗菌株,根据形态学、生理生化特性及多基因测序进行菌株种类鉴定,通过离体果实和盆栽试验明确供试菌株的防病促生效果。结果表明,芽胞杆菌JZB30-1对番茄灰霉病菌等10余种植物病原真菌和细菌具有广谱抑菌活性;该菌株具有合成蛋白酶、纤维素酶、脂肽类物质,分泌铁载体、生长激素及溶磷等特性,经鉴定其为解淀粉芽胞杆菌Bacillus amyloliquefaciens;菌株JZB30-1无菌发酵滤液50倍液喷施处理,对番茄果实灰霉病防效为95.0%,发酵液10倍稀释液灌根,番茄株高、鲜重分别较对照增加25.7%和28.4%。相关结果为利用解淀粉芽胞杆菌JZB30-1进行番茄灰霉病生物防治提供理论依据。

关键词：番茄灰霉病解淀粉芽胞杆菌抑菌活性功能基因生物防治

Agricultural Vulnerability to Drought in China's Agro-pastoral Ecotone:A Case Study of Yulin City,Shaanxi Province

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中国地理科学:英文版 2023年第5期33卷 934-945页

作者： LI Yuheng CHENG Wenjing zuo wenjie ZHANG Lingyue Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing 100101China College of Resources and Environment University of the Chinese Academy of SciencesBeijing 100049China College of Public Administration and Law Northeast Agricultural UniversityHarbin 150030China College of Economics and Management Northwest A&F UniversityYangling 712100China

Agro-pastoral ecotone of northern China is the prominent area for agricultural production,but it is also the most typical ecological fragile area with frequent drought *** Yulin City at Shaanxi Province in China as the case area,the paper aims to investigate the spatio-temporal changes of agricultural vulnerability to drought in China’s agro-pastoral ecotone in the period 2000 *** results show that:1)the agricultural vulnerability to drought in Yulin City has shifted from high vulnerability in the period2000–2010 to low vulnerability in the period 2011–2020.2)There exist obvious spatio-temporal differences of the agricultural vulnerability to drought in Yulin City during the research period.3)Four sensitive events and 14 resilient events were identified in the research and the crops of Yulin had become more resilient to ***,the paper put forward with policy implications to make adaptive strategies of agriculture to climate change in China’s agro-pastoral ecotone in the future,e.g.,carrying out agricultural zoning based on agricultural production conditions,intensifying the construction of disaster prevention and relief system,and integrating with modern agricultural technology to develop new type agriculture.

关键词： climate change agricultural vulnerability drought agro-pastoral ecotone Yulin City,China

An adaptive reanalysis method for genetic algorithm with application to fast truss optimization

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Acta Mechanica Sinica 2010年第2期26卷 225-234页

作者： Tao Xu wenjie zuo Tianshuang Xu Guangcai Song Ruichuan Li State Key Laboratory of Automotive Dynamic Simulation Jilin University 130025 Changchun China Department of Mechanics Jilin University 130025 Changchun China

Although the genetic algorithm （GA） for structural optimization is very robust, it is very computationally intensive and hence slower than optimality criteria and mathematical programming methods. To speed up the design process, the authors present an adaptive reanalysis method for GA and its applications in the optimal design of trusses. This reanalysis technique is primarily derived from the Kirsch＇s combined approximations method. An iteration scheme is adopted to adaptively determine the number of basis vectors at every generation. In order to illustrate this method, three classical examples of optimal truss design are used to validate the proposed reanalysis-based design procedure. The presented numerical results demonstrate that the adaptive reanalysis technique affects very slightly the accuracy of the optimal solutions and does accelerate the design process, especially for large-scale structures.

关键词： Truss structure Adaptive reanalysis ·Genetic algorithm ·Fast optimization

Insight into structure evolution of carbon nitrides and its energy conversion as luminescence

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Carbon Energy 2024年第2期6卷 37-60页

作者： Hao Zhang Jingwei Zhang wenjie Chen Minjia Tao Xianguang Meng Yuanjian Zhang Guifu zuo Hebei Provincial Key Laboratory of Inorganic Nonmetallic Materials College of Materials Science and EngineeringNorth China University of Science and TechnologyTangshanHebeiChina College of Clinical Medicine North China University of Science and TechnologyTangshanHebeiChina Hebei Iron and Steel Laboratory TangshanHebeiChina Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical ResearchState Key Laboratory of BioelectronicsSchool of Chemistry and Chemical EngineeringMedical SchoolSoutheast UniversityNanjingJiangsuChina

A series of carbon nitride(CN)materials represented by graphitic carbon nitride(g-C_(3)N_(4))have been widely used in bioimaging,biosensing,and other fields in recent years due to their nontoxicity,low cost,and high luminescent quantum *** is more attractive is that the luminescent properties such as wavelength and intensity can be regulated by controlling the structure at the molecular ***,it is time to summarize the related research on CN structural evolution and make a prospect on future *** this review,we first summarize the research history and multiple structural evolution of ***,the progress of improving the luminescence performance of CN through structural evolution was ***,the relationship between CN structure evolution and energy conversion in the forms of photoluminescence,chemiluminescence,and electrochemiluminescence was ***,key challenges and opportunities such as nanoscale dispersion strategy,luminous efficiency improving methods,standardization evaluation,and macroscopic preparation of CN are highlighted.

关键词： carbon nitride chemiluminescence electrochemiluminescence energy conversion photoluminescence structural evolution

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考虑冲压工艺约束的车身骨架断面形状优化

汽车工程 2018年第8期40卷 897-903,966页

作者：桂春阳鞠伟左文杰吉林大学汽车仿真与控制国家重点实验室长春130022 吉林大学机械科学与工程学院长春130022 中国第一汽车股份有限公司技术中心长春130011

在汽车概念设计阶段,为快速评价车身性能,车身常由薄壁梁搭建而成,本文中对其关键技术薄壁梁复杂断面的形状设计进行研究。首先,推导了开口、单室、双室、三室和四室等复杂断面的力学特性计算公式。其次,对冲压工艺约束,包括最短线段、... 详细信息

在汽车概念设计阶段,为快速评价车身性能,车身常由薄壁梁搭建而成,本文中对其关键技术薄壁梁复杂断面的形状设计进行研究。首先,推导了开口、单室、双室、三室和四室等复杂断面的力学特性计算公式。其次,对冲压工艺约束,包括最短线段、拔模角、倒圆角半径,和装配条件等约束进行了数学定量描述。再次,在弯、扭惯性矩和冲压工艺约束下,对断面形状与板厚进行了优化,实现了断面面积最小化。最后,通过算例验证了该方法的有效性。

关键词：车身断面概念设计薄壁梁冲压工艺形状优化

基于等效静态载荷法的汽车前端结构抗撞性尺寸和形貌优化

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振动与冲击 2018年第7期37卷 56-61页

作者：陆善彬蒋伟波左文杰吉林大学汽车仿真与控制国家重点实验室长春130022 吉林大学汽车工程学院长春130022 吉林大学机械科学与工程学院长春130022

为了减少在低速正碰下汽车的维修成本以及实现汽车前端结构的轻量化。采用等效静态载荷方法对汽车前端结构的尺寸和形状进行了抗撞性优化设计,以整个结构质量最小为优化的目标函数,以前部结构主要部件的厚度尺寸以及节点坐标形貌为设计... 详细信息

为了减少在低速正碰下汽车的维修成本以及实现汽车前端结构的轻量化。采用等效静态载荷方法对汽车前端结构的尺寸和形状进行了抗撞性优化设计,以整个结构质量最小为优化的目标函数,以前部结构主要部件的厚度尺寸以及节点坐标形貌为设计变量,以侵入量以及加强筋的冲压工艺要求为约束进行了碰撞优化设计。等效静态载荷法将非线性瞬态碰撞优化问题转化为多工况线性静态优化问题,数值算例证明等效静态载荷法可高效地求解碰撞优化问题,并得到高精度的最优解。

关键词：汽车前端结构碰撞优化尺寸与形貌优化等效静态载荷法

考虑冲压工艺的前纵梁前端结构碰撞模型的标定

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汽车工程 2016年第5期38卷 561-566页

作者：左文杰白建涛李亦文吉林大学汽车仿真与控制国家重点实验室长春130022 汽车零部件先进制造技术教育部重点实验室(重庆理工大学) 重庆400054 吉林大学机械科学与工程学院长春130022 中国第一汽车股份有限公司技术中心长春130011

采用自主开发的SuperSection软件建立了前纵梁前端结构的简化模型,并通过碰撞力标定模型确定薄壁梁的最优断面形状。以最大峰值碰撞力和平均碰撞力与对标值的残差最小为目标,以吸能和冲压工艺要求为约束,以断面节点坐标和薄板厚度为设... 详细信息

采用自主开发的SuperSection软件建立了前纵梁前端结构的简化模型,并通过碰撞力标定模型确定薄壁梁的最优断面形状。以最大峰值碰撞力和平均碰撞力与对标值的残差最小为目标,以吸能和冲压工艺要求为约束,以断面节点坐标和薄板厚度为设计变量建立标定模型,采用遗传算法求解该标定模型。数值算例表明:随着迭代的进行,断面形状逐渐被改进,碰撞力曲线趋近于对标曲线,碰撞中吸收的能量增加且满足冲压工艺要求。

关键词：前纵梁碰撞模型标定形状优化遗传算法