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Transcriptome Analysis of the Digestive Tract of Tachypleus tridentatus and Carcinoscorpius rotundicauda

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Journal of Ocean University of China 2022年第3期21卷 591-600页

作者： LI Yuhong KWAN Kit Yue LI Sansu LIU Yingchun weng Bosen HUANG Wenshu weng zhaohong Department of Environmental Science and Engineering Huaqiao UniversityXiamen361021China College of Marine Sciences Beibu Gulf UniversityQinzhou535011China School of Biomedical Sciences Huaqiao UniversityXiamen361021China School of Basic Medical Sciences Fujian Medical UniversityFuzhou350108China Fisheries College Jimei UniversityXiamen361021China

Transcriptome assemblies for the stomach,midgut,and fecal mucosa of Tachypleus tridentatus and Carcinoscorpius rotundicauda are reported for the first time in this *** assembly resulted in 373069 unigenes with an N50 of 1314,while transcriptome assembly resulted in 736378 unigenes and an N50 of *** annotated unigenes showed the highest similarity to fishes,amphibians,and reptiles,with most unigenes closely related to metabolism,translation,biogenesis,signal transduction,energy production,immune response,and ***(4768)were mapped to KEGG pathways,and the most enriched pathways were involved in translation,environmental information signal processing,metabolism,endocrine system,immune system,nervous system,and varying *** 65889,2001,and 7162 differentially expressed genes(DEGs)were identified in the stomach,midgut,and fecal mucosa between the two horseshoe crab species,*** with ***,99.95%of the DEGs in the *** stomach were down-regulated,while 99.97%of the DEGs of *** fecal mucosa and 55.42%of the DEGs of the *** midgut were *** midgut DEGs were involved in hydrolase activity,protein metabolism,and cell cytoplasm,while most stomach and fecal mucosa DEGs were involved in catalytic activity and primary metabolic *** stomach DEGs were assigned to the cellular component in cellular macromolecular complexes,while most fecal mucosa DEGs were assigned to the cellular component in cell and cell *** results will benefit the investigation of the molecular mechanisms of the digestive tract related to the feeding habits and environmental traces of horseshoe crabs.

关键词： transcriptome digestive tract Tachypleus tridentatus Carcinoscorpius rotundicauda differentially expressed genes(DEGs)

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温度对中国鲎幼体蜕壳及成活的影响

中国水产科学 2024年第6期31卷 674-683页

作者：翁朝红陈瑞芳谢仰杰陈志钟赟民臧浩男吴思颖集美大学水产学院海水养殖生物育种全国重点实验室农业农村部东海海水健康养殖重点实验室广西科学院广西海洋生物种质资源利用技术创新中心福建省淡水水产研究所

为了评价温度对中国鲎(Tachypleus tridentatus)幼体培育效果的影响,在实验室条件下利用自行设计的中国鲎幼体培育系统,比较了仔鲎和稚鲎在4种水温(25、28、31和34℃)条件下的蜕壳率、蜕壳时间和死亡率。结果表明,经过120d的培育,仔稚... 详细信息

为了评价温度对中国鲎(Tachypleus tridentatus)幼体培育效果的影响,在实验室条件下利用自行设计的中国鲎幼体培育系统,比较了仔鲎和稚鲎在4种水温(25、28、31和34℃)条件下的蜕壳率、蜕壳时间和死亡率。结果表明,经过120d的培育,仔稚鲎的总存活率在各温度组之间无显著差异(P>0.05),但较高培育温度能显著促进仔稚鲎蜕壳。实验结束时, 34℃组多数幼体为4龄稚鲎,部分已进入5龄; 31℃组多数为4龄稚鲎; 28℃组多数为3龄稚鲎,部分进入4龄;25℃组多数为2龄稚鲎,仅少数个体进入3龄。在实验期间内,1龄仔鲎的死亡率为6.00%～19.33%,其中25℃温度组1龄仔鲎的死亡率显著高于31℃和34℃温度组(P0.05); 2～4龄稚鲎的死亡率在不同温度组之间无显著差异(P>0.05)。1龄仔鲎最早蜕壳时间在不同温度组之间差异显著(P<0.05),水温越高,开始出现蜕壳的时间越早。根据各龄幼体蜕壳的加权平均时间和最早蜕壳时间推测,在25～34℃条件下,2～4龄幼体各龄期发育所需时间介于27～43d。1龄期的发育时间受温度的影响显著大于2龄以上幼体。综合分析,在适宜温度范围内,水温升高能有效促进中国鲎仔鲎和稚鲎蜕壳, 31℃和34℃均能获得很好的培育效果。结果提示,在室内大规模培育大规格稚鲎是可行的,鲎卵孵出1龄仔鲎后,应及时采用较高温度进行培育,使其能尽快发育蜕壳进入2龄,避免低温导致仔鲎进入滞育状态。

关键词：中国鲎仔鲎稚鲎温度培育

Genetic difference of Chinese horseshoe crab(Tachypleus tridentatus) in southeast coast of China based on mitochondrial COI gene analysis

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Acta Oceanologica Sinica 2012年第3期31卷 132-137页

作者： weng zhaohong XIAO Zhiqun XIE Yangjie WANG Zhiyong GUI Jianfang Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture of the P.R.C.Fisheries CollegeJimei UniversityXiamen 361021China Graduate School of Chinese Academy of Sciences Beijing 100039China

Population genetic structure and historical demography of Chinese horseshoe crab (***)along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit Ⅰ (COI) *** sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities:Ninghai in Zhejiang Province,Meizhou and Zhangpu in Fujian Province,Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan *** variation was relatively low with a total of seven transitions *** all localities,Haplotype H3 was the dominant type observed among eight haplotypes defined previously,and was at the center of radiation in Median-Joining *** prolonged star-like network suggests a signature of population *** level of diversity was low in total,with haplotype diversity ( Hd) being equal to 0.765 and nucleotide diversity (π) being equal to 0.00118,*** genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou *** mismatch distribution analysis and Fu's Fs test provided consistent inference of historic population *** low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal.

关键词： Tachypleus tridentatus genetic difference cytochrome c oxidase subunit I gene mtDNA

Organic ferroelastic enantiomers with high Tc and large dielectric switching ratio triggered by order-disorder and displacive phase transition

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Chinese Chemical Letters 2024年第10期35卷 228-232页

作者： zhaohong Chen Mengzhen Li Jinfei Lan Shengqian Hu Xiaogang Chen Ordered Matter Science Research Center Nanchang UniversityNanchang 330031China

Molecular-based ferroelastics with dielectric switching properties are highly desirable for their applications on microelectronic dielectric switches,sensors,data storage,and so ***,the current reports mostly focus on organic-inorganic hybrids containing toxic heavy metal atoms,and the relatively low phase transition temperature limits their *** this paper,low-toxic organic salt ferroelastic enantiomers(R/S)-4-fluoro-1-azabicyclo[3.2.1]octonium chloride[(R/S)-F-321]were designed and synthesized under the introducing chirality *** undergo a 432F422-type ferroelastic phase transition with a high Curie temperature(Tc)of 470 K,simultaneously exhibiting excellent dielectric switching *** addition to the ordered-disordered movement of cations,the significant displacement of anions is also responsible for such high Tc and large dielectric switching ratios,which is very rare in molecular-based switching *** work enriches the development of molecular ferroelastic switching materials and gives inspiration for the exploration of environmentally friendly high Tc organic salt ferroelastics with prominent switching performances.

关键词： Organic salt Enantiomers Ferroelastic Dielectric switching Phasetransition

Prognostic Biomarkers for Hepatocellular Carcinoma Based on Serine and Glycine Metabolism-related Genes

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Journal of Clinical and Translational Hepatology 2024年第3期12卷 266-277页

作者： Xufan Cai Fang Xu zhaohong Wang Hui Chen Shengzhang Lin Department of Clinical Medicine School of MedicineHangzhou City UniversityHangzhouZhejiangChina Zhejiang Chinese Medical University HangzhouZhejiangChina The First Affiliated Hospital of Bengbu Medical College BengbuAnhuiChina Department of Hepatobiliary and Pancreatic Surgery The Second Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiangChina

Background and Aims:Targeted therapy and immunotherapy have emerged as treatment options for hepatocellular carcinoma(HCC)in recent *** significance of serine and glycine metabolism in various cancers is widely *** study aims to investigate their correlation with the prognosis and tumor immune microenvironment(TIME)of ***:Based on the public database,different subtypes were identified by cluster analysis,and the prognostic model was constructed through regression *** gene expression omnibus(GEO)data set was used as the validation set to verify the performance of the *** survival curve evaluated prognostic *** was used to evaluate the level of immune cell infiltration,and maftools analyzed the *** screened small molecule compounds related to prognostic ***:HCC was found to have two distinct ***,we constructed a risk score prognostic model through regression analysis based on serine and glycine metabolismrelated genes(SGMGs).A nomogram was constructed based on risk scores and other clinical *** patients with a higher risk score showed a poor prognosis,and there were significant differences in immune cell infiltration between the high-and low-risk *** addition,three potential drugs associated with prognostic genes,streptozocin,norfloxacin,and hydrocotarnine,were ***:This study investigated the expression patterns of SGMGs and their relationship with tumor characteristics,resulting in the development of a novel model for predicting the prognosis of HCC *** study provides a reference for clinical prognosis prediction and treatment of HCC patients.

关键词： Serine and glycine metabolism-related genes Hepatocellular carcinoma Risk group Prognosis Tumor immune microenvironment

Resource Allocation to Minimize the Makespan with Multi-Resource Operations

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Journal of Systems Science & Complexity 2024年第5期37卷 2054-2070页

作者： weng Wuyan CHU Chengbin WU Peng School of Economics and Management Fuzhou UniversityFuzhou 350108China Univ Gustave Eiffel ESIEE Paris&COSYS-GRETTIAF-77454 Marne-la-ValléeFrance

This paper investigates a new resource-allocation problem involving multi-resource operations,where completing an operation requires simultaneous use of multiple(renewable)resources,probably of different *** goal of the study is to provide a solution method that minimizes the *** authors formulate the problem into a novel mixed-integer linear program(MILP)*** efficiently solve practical-sized instances,an exact Benders decomposition algorithm is *** algorithm divides the original problem into a master problem of allocating resources and a subproblem of calculating the makespan,and both are linked via Benders *** convergence is sped up by improving the mathematical model and embedding the variable neighborhood search *** with CPLEX,a commonly used MILP solver,the computational results demonstrate that the proposed algorithm provides tighter upper and lower bounds in most *** particular,compared with CPLEX,the proposed method can on average improve the upper and lower bounds by 4.76%and 4.39%,respectively,in solving practical-sized instances.

关键词： Benders decomposition multi-resources operations resource allocation

Advancements in personalized stem cell models for aging-related neurodegenerative disorders

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Neural Regeneration Research 2024年第11期19卷 2333-2334页

作者： Mingxi weng Ralf Jauch School of Biomedical Sciences Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionChina Center for Translational Stem Cell Biology Hong Kong Special Administrative RegionChina

Neurodegenerative diseases(NDDs)are a class of disorders characterized by the gradual loss or malfunction of specific cell populations in the nervous system,which can be triggered by genetic or environmental *** a result,patients often experience a decline in mobility,sensation,memory,and cognition,which can ultimately lead to a fatal *** global incidence of NDDs,including Alzheimer’s disease,Parkinson’s disease,Huntington’s disease,amyotrophic lateral sclerosis(ALS),and multiple sclerosis,is increasing.

关键词： degenerative Huntington sensation

SETD2 in cancer:functions,molecular mechanisms,and therapeutic regimens

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Cancer Biology & Medicine 2024年第9期21卷 725-730页

作者： Yawen weng Jing Xue Ningning Niu State Key Laboratory of Systems Medicine for Cancer Stem Cell Research CenterRen Ji HospitalShanghai Cancer InstituteShanghai Jiao Tong University School of MedicineShanghai 200127China

In recent years,the histone methyltransferase SET domain containing 2(SETD2)has garnered significant attention for its involvement in *** we aim to summarize the research advances regarding SETD2 in tumors,elucidate the role in global epigenetic regulation,highlight potential therapeutic regimens for patients with SETD2 deficiency,and outline future research directions.

关键词： SETD2 regimen therapeutic

Vector Radiative Transfer in a Vertically Inhomogeneous Scattering and Emitting ***Ⅰ:A New Discrete Ordinate Method

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Journal of Meteorological Research 2024年第2期38卷 209-224页

作者： Ziqiang ZHU Fuzhong weng Yang HAN Chinese Academy of Meteorological Sciences China Meteorological AdministrationBeijing 100081 University of Chinese Academy of Sciences Beijing 100049 CMA Earth System Modeling and Prediction Centre China Meteorological Administration(CMA)Beijing 100081 State Key Laboratory of Severe Weather Chinese Academy of Meteorological SciencesChina Meteorological AdministrationBeijing 100081

The original vector discrete ordinate radiative transfer(VDISORT)model takes into account Stokes radiance vector but derives its solution assuming azimuthal symmetric surface reflective matrix and atmospheric scattering phase matrix,such as the phase matrix derived from spherical particles or randomly oriented non-spherical *** this study,a new VDISORT is developed for general atmospheric scattering and boundary *** vector is decomposed into both sinusoidal and cosinusoidal harmonic modes,and the radiance at arbitrary viewing geometry is solved directly by adding two zero-weighted points in the Gaussian quadrature *** complex eigenvalues in homogeneous solutions are also taken into full *** accuracy of VDISORT model is comprehensively validated by four cases:Rayleigh scattering case,the spherical particle scattering case with the Legendre expansion coefficients of 0th-13th orders of the phase matrix(hereinafter L13),L13 with a polarized source,and the randomoriented oblate particle scattering case with the Legendre expansion coefficients of 0th-11th orders of the phase matrix(hereinafter L11).In all cases,the simulated radiances agree well with the benchmarks,with absolute biases less than 0.0065,0.0006,and 0.0008 for Rayleigh,unpolarized L13,and L11,*** a polarized bidirectional reflection distribution function(pBRDF)matrix is used as the lower boundary condition,VDISORT is now able to handle fully coupled atmospheric and surface polarimetric radiative transfer processes.

关键词： radiative transfer discrete ordinate polarization Stokes vector vector discrete ordinate radiative transfer(VDISORT)