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Human umbilical cord mesenchymal stem cells ameliorate liver fibrosis in vitro and in vivo: From biological characteristics to therapeutic mechanisms

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World Journal of Stem Cells 2019年第8期11卷 548-564页

作者： Fei Yin wen-ying wang wen-Hua Jiang Department of Histology and Embryology Basic Medical College of Jilin University

Liver fibrosis is a wound-healing response to chronic injuries, characterized by the excessive accumulation of extracellular matrix or scar tissue within the liver;in addition, its formation is associated with multiple cytokines as well as several cell types and a variety of signaling pathways. When liver fibrosis is not well controlled, it can progress to liver cirrhosis, but it is reversible in principle. Thus far, no efficient therapy is available for treatment of liver fibrosis. Although liver transplantation is the preferred strategy, there are many challenges remaining in this approach, such as shortage of donor organs, immunological rejection, and surgical complications. Hence, there is a great need for an alternative therapeutic strategy. Currently, mesenchymal stem cell (MSC) therapy is considered a promising therapeutic strategy for the treatment of liver fibrosis;advantageously, the characteristics of MSCs are continuous self-renewal, proliferation, multipotent differentiation, and immunomodulatory activities. The human umbilical cord-derived (hUC)-MSCs possess not only the common attributes of MSCs but also more stable biological characteristics, relatively easy accessibility, abundant source, and no ethical issues (e.g., bone marrow being the adult source), making hUC-MSCs a good choice for treatment of liver fibrosis. In this review, we summarize the biological characteristics of hUC-MSCs and their paracrine effects, exerted by secretion of various cytokines, which ultimately promote liver repair through several signaling pathways. Additionally, we discuss the capacity of hUC-MSCs to differentiate into hepatocyte-like cells for compensating the function of existing hepatocytes, which may aid in amelioration of liver fibrosis. Finally, we discuss the current status of the research field and its future prospects.

关键词： Human umbilical cord mesenchymal stem cells Liver fibrosis Hepatocytelike cells Mechanism Cell therapy Paracrine effect Exosome Transdifferentiation

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Retinal vascular diameter changes assessed with a computer-assisted software after strabismus surgery

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International Journal of Ophthalmology(English edition) 2020年第4期13卷 620-624页

作者： Jin-Qiong Zhou Jing Fu Ji-Peng Li Xiao-Zhen wang wen-ying wang Bo-wen Zhao Meng Qi Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology&Visual Sciences Key LaboratoryBeijing 100730China

AIM: To quantitatively investigate the retinal vascular diameter changes, analyzing the early and long-term effects on the retinal circulation, with 6-month follow-up. METHODS: Patients underwent horizontal strabismus surger y were enrolled prospectively. Retinal vessel diameters on color fundus photographs were assessed before and 1, 7 d, 6 mo after surgery, using a computerassisted quantitative assessment software. To evaluate the retinal vascular caliber changes, retinal vascular diameters were calculated by means of the Parr–Hubbard formula as the central retinal arteriolar equivalent(CRAE) and central retinal venular equivalent(CRVE). The arteriovenous ratio(AVR) was calculated as CRAE divided by CRVE. RESULTS: A total of 154 eyes of 104 consecutive patients were included. Compared with the data before surgery(121.55±24.67), the mean CRAE(131.18±28.29) significantly increased 1 d af ter surger y(P=0.003), but went back to baseline level at 7 d(118.89±30.35, P=0.15), and 6 mo(123.22±15.32, P=0.60), so did the AVR(P<0.001, P=0.08, P=0.07). As for the mean CRVE, there was no significant difference between those four time points(172.43±33.25, 175.57±36.98, 174.03±40.18, 174.86±20.46, P=1.00). CONCLUSION: Strabismus surgery on both lateral and media rectus muscles, or single media rectus muscle may increase retinal blood flow during the early postoperative period, but would return to normal later. The number of transected anterior ciliary arteries rather might be the main cause of retinal hemodynamic changes early after strabismus surgery.

关键词： retinal blood flow retinal vascular diameter strabismus strabismus surgery

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MicroRNA-mediated Silencing of RhoC Inhibits Tumor Invasion and Increases Chemosensitivity to Paclitaxel in SKOV3 Cells in vitro

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Chemical Research in Chinese Universities 2011年第1期27卷 70-74页

作者： PAN ying DU Zhen-wu LENG Wei-chun ZHOU Jia-wen wang ying-jian SHENG Min-jia wang Jun-rong ZHANG Gui-zhen China-Japan Union Hospital of Jilin University Changchun 130031 P. R. China

RhoC is a member of the Ras-homologous family of genes which are implicated in tumorigenesis and tumor progression. Up-regulation of RhoC is associated with tumor progression in ovarian carcinoma and RhoC is significantly correlated with the invasive capability of ovarian cancer cell lines in vitro, We developed a system that blocks RhoC in the human ovarian cancer SKOV3 cells using specific MicroRNA（miRNA） interference. By transfecting SKOV3 cells with the plasmid vector to express specific MiRNA that targets human RhoC, we were able to establish a stable clone in which RhoC expression was significantly downregulated. This resulted in the decreased invasive potential of SKOV3 cells as well as increased chemosensitivity to paclitaxel. RhoC involves in invasion and chemosensitivity of SKOV3, indicating that RhoC may be a promising therapeutic target for ovarian cancer.

关键词： RhoC gene miRNA SKOV3 cell Cell invasion Paclitaxel

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High quality repair of osteochondral defects in rats using the extracellular matrix of antler stem cells

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World Journal of Stem Cells 2024年第2期16卷 176-190页

作者： Yu-Su wang wen-Hui Chu Jing-Jie Zhai wen-ying wang Zhong-Mei He Quan-Min Zhao Chun-Yi Li Institute of Antler Science and Product Technology Changchun Sci-Tech UniversityChangchun 130000Jilin ProvinceChina College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun 130118Jilin ProvinceChina School of Life Science Taizhou UniversityTaizhou 318000Zhejiang ProvinceChina Department of Oral Implantology Hospital of StomatologyJilin UniversityChangchun 130000Jilin ProvinceChina

BACKGROUND Cartilage defects are some of the most common causes of *** lesions caused by inflammation,trauma or degenerative disease normally result in osteochondral *** studies have shown that decellularized extracellular matrix(ECM)derived from autologous,allogenic,or xenogeneic mesenchymal stromal cells(MSCs)can effectively restore osteochondral *** To determine whether the decellularized ECM of antler reserve mesenchymal cells(RMCs),a xenogeneic material from antler stem cells,is superior to the currently available treatments for osteochondral *** We isolated the RMCs from a 60-d-old sika deer antler and cultured them in vitro to 70%confluence;50 mg/mL L-ascorbic acid was then added to the medium to stimulate ECM *** sheets of adipocyte-derived MSCs(aMSCs)and antlerogenic periosteal cells(another type of antler stem cells)were used as the *** weeks after ascorbic acid stimulation,the ECM sheets were harvested and applied to the osteochondral defects in rat knee *** The defects were successfully repaired by applying the *** highest quality of repair was achieved in the RMC-ECM group both in vitro(including cell attachment and proliferation),and in vivo(including the simultaneous regeneration of well-vascularized subchondral bone and avascular articular hyaline cartilage integrated with surrounding native tissues).Notably,the antler-stem-cell-derived ECM(xenogeneic)performed better than the aMSC-ECM(allogenic),while the ECM of the active antler stem cells was superior to that of the quiescent antler stem *** Decellularized xenogeneic ECM derived from the antler stem cell,particularly the active form(RMC-ECM),can achieve high quality repair/reconstruction of osteochondral defects,suggesting that selection of decellularized ECM for such repair should be focused more on bioactivity rather than kinship.

关键词： Osteochondral defect repair Mesenchymal stem cells Extracellular matrix Decellularization Antler stem cells Reserve mesenchymal cells Xenogeneic

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Clinical experiences in the management of critically ill patients with COVID‑19 in a designated children’s hospital in China

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World Journal of Pediatrics 2023年第6期19卷 605-608页

作者： Gong-Bao Liu ying Gu ying-wen wang Chuan-Qing wang Jian Ma Mei Zeng Guo-Ping Lu Zhong-Lin wang Ai-Mei Xia Jin-Hao Tao Xiao-wen Zhai wen-Hao Zhou Hong Xu Yong-Hao Gui Guo-ying Huang Xiao-Bo Zhang Department of Medical Affairs Children’s Hospital of Fudan UniversityShanghaiChina Department of Nursing Children’s Hospital of Fudan UniversityShanghaiChina Department of Nosocomial Infection Prevention and Control Children’s Hospital of Fudan UniversityShanghaiChina Department of Infectious Disease Children’s Hospital of Fudan UniversityShanghaiChina Department of Critical Medicine Children’s Hospital of Fudan UniversityShanghaiChina Department of Hematology Children’s Hospital of Fudan UniversityShanghaiChina Department of Neonate Children’s Hospital of Fudan UniversityShanghaiChina Department of Nephrology Children’s Hospital of Fudan UniversityShanghaiChina Department of Key Laboratory of Ministry of Health for Neonatal Disease Children’s Hospital of Fudan UniversityShanghaiChina Department of Cardiology Children’s Hospital of Fudan UniversityWanyuan RdNo.399ShanghaiChina Department of Respiratory Children’s Hospital of Fudan UniversityWanyuan RdNo.399ShanghaiChina

Omicron variant has been the dominant epidemic variant of novel coronavirus in the world since *** have shown that the incidence of severe or critical cases with coronavirus disease 2019(COVID-19)is much lower than that of the previous novel coronavirus variants[1,2];however,it is still a major challenge to treat critical pediatric patients with COVID-19,especially for those with underlying diseases.

关键词： patients Clinical cases

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Current Mycoplasma pneumoniae epidemic among children in Shanghai:unusual pneumonia caused by usual pathogen

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World Journal of Pediatrics 2024年第1期20卷 5-10页

作者： Xiao-Bo Zhang wen He Yong-Hao Gui Quan Lu Yong Yin Jian-Hua Zhang Xiao-Yan Dong ying-wen wang ying-Zi Ye Hong Xu Jia-Yu wang Bing Shen Dan-Ping Gu Li-Bo wang Yi wang Department of Respiratory Medicine Children's Hospital of Fudan University399 Wanyuan RoadShanghai 201102China State Key Laboratory of Genetic Engineering at School of Life Sciences Institute of Reproduction&DevelopmentChildren's Hospital of Fudan UniversityShanghaiChina Department of Respiratory Medicine Children's Hospital of Shanghai Jiao Tong UniversityShanghaiChina Department of Respiratory Medicine Shanghai Children's Medical CenterAffiliated to Shanghai Jiaotong University School of MedicineShanghaiChina Department of Pediatric Respiratory Xinhua HospitalAffiliated to Shanghai Jiaotong University School of MedicineShanghaiChina Department of Nursing Children's Hospital of Fudan UniversityShanghaiChina Department of Infectious Diseases Children's Hospital of Fudan UniversityShanghaiChina Department of Nephrology Children's Hospital of Fudan UniversityShanghaiChina NHC Key Laboratory of Neonatal Diseases Children's Hospital of Fudan UniversityShanghaiChina Shanghai Shenkang Hospital Development Center ShanghaiChina Center for Medical Quality Control Management of Shanghai ShanghaiChina Department of Neurology Children's Hospital of Fudan University399Wanyuan RoadShanghai 201102China

Mycoplasma pneumoniae(***),primarily transmitted through respiratory droplets when infected individuals cough or sneeze,is a common cause of communityacquired pneumonia,especially among school-age children and *** infection occurs endemically with an epidemic peak every few *** worldwide incidence confirmed by direct test methods was reported to be 8.61%between 2017 and 2020 across all age groups[1].

关键词： pneumonia epidemic respiratory

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Contrast-enhanced ultrasound using SonoVue mixed with oral gastrointestinal contrast agent to evaluate esophageal hiatal hernia: Report of three cases and a literature review

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World Journal of Clinical Cases 2021年第11期9卷 2679-2687页

作者： Jing-Yu wang Yan Luo wen-ying wang Shi-Cheng Zheng Lian He Chun-Yan Xie Li Peng Department of Ultrasound First People's Hospital of Longquanyi DistrictLongquan Hospital of West China Hospital of Sichuan UniversityChengdu 610100Sichuan ProvinceChina Department of Ultrasound West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Gastroenterology First People's Hospital of Longquanyi DistrictLongquan Hospital of West China Hospital of Sichuan UniversityChengdu 610100Sichuan ProvinceChina

BACKGROUND Due to a thicker abdominal wall in some patients,ultrasound artifacts from gastrointestinal gas and surrounding tissues can interfere with routine ultrasound examination,precluding its ability to display or clearly show the structure of a hernial sac(HS)and thereby diminishing diagnostic performance for esophageal hiatal hernia(EHH).Contrast-enhanced ultrasound(CEUS)imaging using an oral agent mixture allows for clear and intuitive identification of an EHH sac and dynamic observation of esophageal *** SUMMARY In this case series,we report three patients with clinically-suspected EHH,including two females and one male with an average age of 67.3±16.4 *** was administered with an oral agent mixture(microbubble-based SonoVue and gastrointestinal contrast agent)and identified a direct sign of supradiaphragmatic HS(containing the hyperechoic agent)and indirect signs[e.g.,widening of esophageal hiatus,hyperechoic mixture agent continuously or intermittently reflux flowing back and forth from the stomach into the supradiaphragmatic HS,and esophagus-gastric echo ring(i.e.,the“EG”ring)seen above the diaphragm].All three cases received a definitive diagnosis of EHH by esophageal manometry and *** lesions resolved upon drug treatment and one required *** recurrence rate in follow-up was 0%.The data from these cases suggest that the new non-invasive examination method may greatly improve the diagnosis of *** CEUS with the oral agent mixture can facilitate clear and intuitive identification of HS and dynamic observation of esophageal reflux.

关键词： SonoVue Oral gastrointestinal contrast agent Contrast-enhanced ultrasound Gastrointestinal Hiatal hernia Case report

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Voxel-based meta-analysis of grey matter changes in Alzheimer’s disease

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Translational Neurodegeneration 2015年第1期4卷 39-47页

作者： wen-ying wang Jin-Tai Yu Yong Liu Rui-Hua Yin Hui-Fu wang Jun wang Lin Tan Joaquim Radua Lan Tan Department of Neurology Qingdao Municipal HospitalSchool of MedicineQingdao UniversityNo.5 Donghai Middle RoadQingdaoShandong Province 266071China College of Medicine and Pharmaceutics Ocean University of ChinaQingdao 266011China Department of Neurology Qingdao Municipal HospitalNanjing Medical UniversityNanjing 266071China Brainnetome Center Institute of AutomationChinese Academy of SciencesBeijing 100190China National Laboratory of Pattern Recognition Institute of AutomationChinese Academy of SciencesBeijing 100190China Department of Psychosis Studies Institute of PsychiatryKing’s College LondonLondonUK Research Unit FIDMAG Germanes Hospitala’ries—CIBERSAMSant Boi de LlobregatBarcelonaSpain

Background:Voxel-based morphometry(VBM)using structural brain MRI has been widely used for the assessment of impairment in Alzheimer’s disease(AD),but previous studies in VBM studies on AD remain ***:We conducted meta-analyses to integrate the reported studies to determine the consistent grey matter alterations in AD based on VBM ***:The PubMed,ISI Web of Science,EMBASE and Medline database were searched for articles between 1995 and June *** searches were also conducted,and authors of studies were contacted for additional *** were extracted from clusters with significant grey matter difference between AD patients and healthy controls(HC).Meta-analysis was performed using a new improved voxel-based meta-analytic method,Effect Size Signed Differential Mapping(ES-SDM).Results:Thirty data-sets comprising 960 subjects with AD and 1195 HC met inclusion *** matter volume(GMV)reduction at 334 coordinates in AD and no GMV increase were found in the current *** reductions in GMV were robustly localized in the limbic regions(left parahippocampl gyrus and left posterior cingulate gyrus).In addition,there were GM decreases in right fusiform gyrus and right superior frontal *** findings remain largely unchanged in the jackknife sensitivity ***:Our meta-analysis clearly identified GMV atrophy in *** findings confirm that the most prominent and replicable structural abnormalities in AD are in the limbic regions and contributes to the understanding of pathophysiology underlying AD.

关键词： Voxel-based morphometry(VBM) Alzheimer’s disease(AD) Grey matter(GM) Meta-analysis Magnetic resonance imaging(MRI) Effect size signed differential mapping(ES-SDM)

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Retinal vessels caliber changes after strabismus surgery：results of 6mo follow-up

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International Journal of Ophthalmology(English edition) 2016年第9期9卷 1325-1328页

作者： Ji-Peng Li Jing Fu Jin-Qiong Zhou Xiao-Zhen wang wen-ying wang Ning-Pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical University Beijing Ophthalmology &Visual Sciences Key Lab Beijing 100005 China

AIM：To evaluate the effect of strabismus surgery on retinal vessels calibers with digital color fundus ***：Two hundred consecutive strabismus patients underwent surgery,and 118 patients（female/male,55/63） who finished 6-month follow-up were finally included in this *** disc-centered digital color fundus photographs of both eyes of all patients were taken prior to surgery and 6mo post *** retinal vascular caliber of 116 operated eyes were measured using the computer program *** operated eyes were divided into 3 groups according to the surgical methods,recession of one muscle,one muscle recession and one muscle folding,one muscle resection and one muscle *** effect of number of altered muscles on retinal vessels was analyzed using statistic software SPSS ***：The mean age was 12.4±*** central retinal artery equivalent（CRAE） of all patients was 120.31 ±23.02 μm preoperatively,and 122.87 ±15.93 μm six months after *** central retinal vein equivalent（CRVE） was 171.11 ±31.73 μm preoperatively and175.02±21.00 μm *** was no significant difference of averaged CRAE（P=0.22） or CRVE（P=0.19）before and after *** arteriole to venule ratio（AVR） was 0.71 ±0.07 before surgery and 0.70±0.07 after *** of preoperative and postoperative retinal vessels calibers among different surgical groups did not show significant ***,there was no advantage of rectus muscle folding to muscle ***：Up to 6mo after strabismus surgery,the retinal vascular calibers were not *** more than two muscles in one surgery are safe for retinal perfusion.

关键词： strabismus retinal vascular caliber image processing computer-assisted

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Intravitreal injection of conbercept for diabetic macular edema complicated with diabetic nephropathy

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 304-310页

作者： Yuan-Zhang Zhu Zhi-Zhi Dou wen-ying wang Qing-Yue Ma wen-Dan Yi Ning-Ning Yao Yi-Chong Liu Xiao-Di Gao Qian Zhang wen-Juan Luo Department of Ophthalmology the Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

AIM:To observe the therapeutic effect of conbercept on diabetic macular edema(DME)complicated with diabetic nephropathy(DN).METHODS:In this retrospective study,54 patients(54 eyes)that diagnosed as DME from January 2017 to October 2021 were *** patients were divided into two groups:DME patients with DN(25 eyes),and DME patients without DN(29 eyes).General conditions were collected before treatment,laboratory tests include fasting blood glucose,HbA1c,microalbumin/creatinine,serum *** coherence tomography(OCT)was used to check the ellipsoidal zone(EZ)and external limiting membrane(ELM)*** macular thickness(CMT),best corrected visual acuity(BCVA),and retinal hyperreflective foci(HF)as well as numbers of injections were ***:There were significant differences between fasting blood glucose,HbA1c,serum creatinine,urinary microalbumin/creatinine,and estimated glomerular filtration rate(eGFR)between the two groups(all P<0.05).EZ and ELM continuity in the DME+DN group was worse than that in the DME group(P<0.05).BCVA(logMAR)in the DME group was significantly better than that in the DME+DN group at the same time points during treatment(all P<0.05).CMT and HF values were significantly higher in the DME+DN group than that in the DME group at the all time points(all P<0.05)and significantly decreased in both groups with time during *** 6mo after treatment,the mean number of injections in the DME+DN and DME group was 4.84±0.94 and 3.79±0.86,***:Conbercept has a significant effect in short-term treatment of DME patients with or without DN,and can significantly ameliorate BCVA,CMT and the number of HF,treatment efficacy of DME patients without DN is better than that of DME patients with DN.

关键词： conbercept diabetic nephropathy diabetic macular edema optical coherence tomography

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