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The long non-coding RNA PILNCR2 increases low phosphate tolerance in maize by interfering with miRNA399-guided cleavage of ZmPHT1s

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Molecular Plant 2023年第7期16卷 1146-1159页

作者： Yafei Wang Zhonghua Wang Qingguo Du Kai Wang Chunqin Zou wen-xue li National Engineering Laboratory for Crop Molecular Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Resources and Environmental Sciences National Academy of Agriculture Green DevelopmentKey Laboratory of Plant-Soil InteractionsMinistry of EducationChina Agricultural UniversityBeijing100193China

Theopen reading regions of ZmPHT1s(inorganic phosphate[Pij transporters)inmaize possess target sites of microRNA399(miR399).However,the relationship between miR399 and ZmPHT1s and its functional importance in response to Pi deficiency remain to be explored.We show here that ZmPHT1;1,ZmPHT1;3,and ZmPHT1;13 are the targets of ZmmiRNA399.We found that a long non-coding RNA,PILNCR2(Pi-deficiency-induced IncRNA 2),is transcribed from the opposing DNA strand of ZmPHT1;1 and predominantly localized in the cytoplasm.A ribonuclease protection assay and an RNA-RNA binding assay showed that PILNCR2 and ZmPHT1s could form the RNA/RNA duplexes in vivo and in vitro.A co-expression assay in N.benthamiana revealed that the PILNCR2/ZmPHT1 RNA/RNA duplexes interfere with miR399-guided cleavage of ZmPHT1 mRNAs.Overexpression of PILNCR2 increased low-Pi tolerance in maize,whereas its knockout and knockdown decreased low-Pi tolerance in maize.Consistently,ZmPHT1;3 and ZmPHT1;13 mRNA abundance was increased in transgenic plants overexpressing PILNCR2 but reduced in its knock-out mutants,suggesting that PILNCR2 positively regulates the mRNA abundance of ZmPHT1;3 and ZmPHT1;13 in maize.Collectively,these results indicate that PILNCR2 plays an important role in maize Pihomeostasisby interfering with miRNA399-guided cleavageof ZmPHT1mRNAs.

关键词： long non-coding RNA miRNA RNA/RNA duplex post-transcriptional regulation maize

Changes of serum inflammatory factors and miR-145 expression in patients with osteoarthritis before and after treatment and their clinical value

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World Journal of Clinical Cases 2019年第19期7卷 2963-2975页

作者： Xiao-Zhen Wang wen-xue li Department of Orthopedics Dongying Shengli Hospital

BACKGROUND Osteoarthritis is a chronic degenerative disease with an incidence of 50%in people over 65 years old and 80%in people over 80 years old worldwide.It is the second leading reason of loss of working capacity after cardiovascular diseases and severely affects the society and families.Therefore,finding biological markers related to the diagnosis and treatment of osteoarthritis is of great significance in clinical practice.AIM To observe the changes and clinical value of serum inflammatory factors and miR-145 expression in patients with osteoarthritis before and after treatment.METHODS Eighty-three patients with knee osteoarthritis(observation group)who were admitted to our hospital from April 2013 to June 2015,and 60 healthy people(control group)during the same period were selected.After 4 wk of treatment,the levels of miR-145,tumor necrosis factor(TNF)-α,interleukin(IL)-6,and IL-10 were compared between the control group and the observation group before treatment.The correlation of miR-145,TNF-α,IL-6,and IL-10 levels with visual analogue scale(VAS),Lysholm,and Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)scores was assessed by Pearson correlation analysis.The correlation of the expression of miR-145,TNF-α,IL-6,and IL-10 with Kellgren-Lawrence(K-L)grades was assessed by Spearman correlation analysis.The critical levels of miR-145,TNF-α,IL-6,and IL-10 in distinguishing different K-L grades were determined by receiver operating characteristic(ROC)curve analysis.RESULTS The expression level of miR-145 in the observation group was significantly higher than that in the control group before treatment(P<0.05).After treatment,the expression level of miR-145 in the observation group was significantly lower than that before treatment(P<0.05).The levels of TNF-αand IL-6 in the observation group were significantly higher than those in the control group(P<0.05),and the level of IL-10 was significantly lower than that in the control group(P<0.05).After treatment,the levels of TNF-αand IL-6 in the

关键词： Osteoarthritis Treatment Inflammatory factor miR-145

Identification and functional characterization of the AGO1 ortholog in maize

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Journal of Integrative Plant Biology 2016年第8期58卷 749-758页

作者： Dongdong Xu Hailong Yang Cheng Zou wen-xue li Yunbi Xu Chuanxiao Xie Institute of Crop Science Chinese Academy of Agricultural Sciences

Eukaryotic Argonaute proteins play primary roles in mi RNA and si RNA pathways that are essential for numerous developmental and biological processes. However, the functional roles of the four Zm AGO1 genes have not yet been characterized in maize（Zea mays L.）. In the present study, Zm AGO1 a was identified from four putative Zm AGO1 genes for further characterization. Complementation of the Arabidopsis ago1-27 mutant with Zm AGO1 a indicated that constitutive overexpression of Zm AGO1 a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype. The expression profiles of Zm AGO1 a under five different abiotic stresses indicated that Zm AGO1 a shares expression patterns similar to those of Argonaute genes in rice, Arabidopsis, and wheat.Further, variation in Zm AGO1 a alleles among diverse maize germplasm that resulted in several amino acid changes revealed genetic diversity at this locus. The present data suggest that Zm AGO1 a might be an important AGO1 ortholog in maize. The results presented provide further insight into the function of ZmAGO1a.

关键词： AGO1 functional complementation maize ZmAGO1a

Meta‐analysis and candidate gene mining of low‐phosphorus tolerance in maize

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Journal of Integrative Plant Biology 2014年第3期56卷 262-270页

作者： Hongwei Zhang Mohammed Shalim Uddin Cheng Zou Chuanxiao Xie Yunbi Xu wen‐xue li Institute of Crop Science National Key Facility of Crop Gene Resources and Genetic Improvement the Chinese Academy of Agricultural Sciences Bangladesh Agricultural Research Institute (BARI) International Maize and Wheat Improvement Center (CIMMYT)

Plants with tolerance to low-phosphorus （P） can grow better under Iow-P conditions, and understanding of genetic mechanisms of Iow-P tolerance can not only facilitate identifying relevant genes but also help to develop Iow-P tolerant cultivars. QTL meta-analysis was conducted after a comprehensive review of the reports on O, TL mapping for Iow-P tolerance-related traits in maize. Meta-analysis pro- duced 23 consensus QTL （cQTL）, 17 of which located in similar chromosome regions to those previously reported to influence root traits. Meanwhile, candidate gene mining yielded 215 genes, 22 of which located in the cQTL regions. These 22 genes are homologous to 14 functionally characterized genes that were found to participate in plant Iow-P tolerance, including genes encoding miR399s, Pi transporters and purple acid phosphatases. Four cQTL loci （cQTL2-L cQTLS-3, cQTL6-2, and cQTL10-2） may play important roles for low-P tolerance because each contains more original QTL and has better consistency across previous reports.

关键词： Candidate gene consensus QTL low-phosphorustolerance maize meta-analysis

Nitrogen supply affects ion homeostasis by modifying root Casparian strip formation through the miR528-LAC3 module in maize

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Plant Communications 2023年第4期4卷 88-98页

作者： Yu Guo Yafei Wang Huan Chen Qingguo Du Zhonghua Wang Xiaoping Gong Qing Sun wen-xue li National Engineering Laboratory for Crop Molecular Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China Qingdao Agricultural University Qingdao 266109China

Although nitrogen(N)is known to affect mineral element homeostasis in plants,the molecular mechanisms of interactions between N and other nutrients remain largely unclear.Wereport here that N supply affects ion homeostasis inmaize.Berberine hemisulfate staining and a propidiumiodide penetration assay showed that N luxury significantly delayed Casparian strip(CS)formation in maize roots.We further demonstrated that N-mediated CS formation in maize was independent of RBOHF-activated H2O2 production.N luxury induced the expression of ZmmiR528 inwhole roots and root tips.Knockdown and loss-of-function ofZmmiR528 promoted CS formation under both N-luxury and N-deficient conditions.Both ZmMIR528a and ZmMIR528b contribute to early CS formation under different N conditions.RNA-seq and real-time RT-PCR analysis demonstrated that ZmLAC3,but not ZmLAC5,responded to N treatments.Consistent with results obtained with ZmmiR528 TM transgenic maize and mir528a/b loss-of-function mutants,transgenic maize overexpressing ZmLAC3 displayed early CS formation under different N conditions.Under field conditions,K,Ca,Mn,Cu,Mg,and Zn concentrations were greater in the ear leaf of ZmLAC3-overexpressing transgenicmaize than in the wild type.These results indicate that ZmmiR528 affects CS formation in maize by regulating the expression of ZmLAC3,and modification of CS formation has the potential to improve maize quality.

关键词： nitrogen ion homeostasis miR528 Casparian strip maize

The development of laser-produced plasma EUV light source

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Chip 2022年第3期1卷 50-60页

作者： De-Kun Yang Du Wang Qiu-Shi Huang Yi Song Jian Wu wen-xue li Zhan-Shan Wang Xia-Hui Tang Hong-Xing Xu Sheng liu Cheng-Qun Gui The Institute of Technological Sciences Wuhan UniversityWuhan 430072China Key Laboratory of Advanced Micro-Structured Materials MOE Institute of Precision Optical EngineeringSchool of Physics Science and EngineeringTongji UniversityShanghai 200092China State Key Laboratory of Precision Spectroscopy East China Normal UniversityShanghai 200241China School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China School of Physics and Technology Wuhan UniversityWuhan 430072China

Extreme ultraviolet lithography(EUVL)has been demonstrated to meet the industrial requirements of new-generation semiconductor fabrication.The development of high-power EUV sources is a long-term critical challenge to the implementation of EUVL in high-volume manufacturing(HVM),together with other technologies such as photoresist and mask.Historically,both theoretical studies and experiments have clearly indicated that the CO 2 laser-produced plasma(LPP)system is a promising solution for EUVL source,able to realize high conversion efficiency(CE)and output power.Currently,ASML’s NXE:3400B EUV scanner configuring CO_(2) LPP source sys-tem has been installed and operated at chipmaker customers.Mean-while,other research teams have made different progresses in the development of LPP EUV sources.However,in their technologies,some critical areas need to be further improved to meet the requirements of 5 nm node and below.Critically needed improvements include higher laser power,stable droplet generation system and longer collector life-time.In this paper,we describe the performance characteristics of the laser system,droplet generator and mirror collector for different EUV sources,and also the new development results.

关键词： Extreme ultraviolet source Laser-produced plasma Sn target Long lifetime collector

MicroRNA528 Affects Lodging Resistance of Maize by Regulating lignin Biosynthesis under Nitrogen-Luxury Conditions

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Molecular Plant 2018年第6期11卷 806-814页

作者： Qing Sun Xiaogang liu Juan Yang wenwen liu Qingguo Du Hongqiu Wang Chunxiang Fu wen-xue li National Engineering Laboratory for Crop Molecular Breeding Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China Shandong Provincial Key Laboratory of Energy Genetics Qingdae Engineering Research Center of Biomass Resources and Environment and CAS Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao 266101 China

Lodging under nitrogen （N）-Iuxury conditions substantially reduces crop yield and seed quality. However, the molecular mechanisms of plant lodging resistance remain largely unclear, especially in maize. We report here that the expression of ZmmiR528, a monocot-specific microRNA, is induced by N luxury but reduced by N deficiency. We show by the thioacidolysis and acetyl bromide analysis that N luxury signifi- cantly reduces the generation of H, G, and S monomers of the lignin as well as its total content in maize shoots. We further demonstrate that ZmLACCASE3 （ZmLAC3） and ZmLACCASE5 （ZmLAC5）, which encode the copper-containing laccases, are the targets of ZmmiR528. In situ hybridization showed that ZmmiR528 is mainly expressed in maize vascular tissues. Knockdown of ZmmiR528 or overexpression of ZmLAC3 significantly increased the lignin content and rind penetrometer resistance of maize stems. In contrast, transgenic maize plants overexpressing ZmmiR528 had reduced lignin content and rind penetrometer resistance and were prone to lodging under N-luxury conditions. RNA-sequencing analysis revealed that ZmPAL7 and ZmPAL8 are upregulated in transgenic maize lines downregulating ZmmiR528. Under N-lux- ury conditions, the expression levels of ZmPALs were much higher in ZmmiR528-knockdown lines than in the wild type and transgenic maize lines overexpressing ZmmiR528. Taken together, these results indicate that, by regulating the expression of ZmLAC3 and ZmLAC5, ZmmiR528 affects maize lodging resistance un- der N-luxury conditions.

关键词： maize nitrogen ZmmiR528 ZmLACs lodging resistance

Identification of traits and genes associated with lodging resistance in maize

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The Crop Journal 2021年第6期9卷 1408-1417页

作者： Yu Guo Yumei Hu Huan Chen Pengshuai Yan Qingguo Du Yafei Wang Hongqiu Wang Zhonghua Wang Dingming Kang wen-xue li Key Laboratory of Crop Heterosis and Utilization Ministry of EducationCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Engineering Laboratory for Crop Molecular Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Lodging is a major problem limiting maize yield worldwide. However, the mechanisms of lodging resistance remain incompletely understood for maize. Here, we evaluated 443 maize accessions for lodging resistance in the field. Five lodging-resistant accessions and five lodging-sensitive accessions were selected for further research. The leaf number, plant height, stem diameter, and rind penetrometer resistance were similar between lodging-resistant and-sensitive inbred lines. The average thickness of sclerenchymatous hypodermis layer was thicker and the vascular area was larger in the lodging-resistant lines compared with lodging-sensitive lines. Although total lignin content in stem tissue did not significantly differ between lodging-resistant and-sensitive lines, phloroglucinol staining revealed that the lignin content of the cell wall in the stem cortex and in the stem vascular tissue near the cortex was higher in the lodging-resistant lines than in the lodging-sensitive lines. Analysis of strand-specific RNA-seq transcriptome showed that a total of 793 genes were up-regulated and 713 genes were down-regulated in lodging-resistant lines relative to lodging-sensitive lines. The up-regulated genes in lodging-resistant lines were enriched in cell wall biogenesis. These results indicated that modification of cell wall biosynthesis would contribute to lodging resistance of maize.

关键词： Lodging Sclerenchyma cell lignin Differentially expressed genes Maize

Multiple skin abscesses associated with bacteremia caused by Burkholderia gladioli:A case report

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World Journal of Clinical Cases 2022年第7期10卷 2286-2293页

作者： Yi-Ting Wang xue-wen li Pan-Yang Xu Chun Yang Jian-Cheng Xu The First Hospital of Jilin University Laboratory MedicineChangchun 130021Jilin ProvinceChina

BACKGROUND Burkholderia gladioli(B.gladioli)is regarded as a rare opportunistic pathogen.Only a few patients with abscesses caused by B.gladioli infections have been reported,and these are usually abscesses at the incision caused by traumatic surgery.CASE SUMMARY A 74-year-old male patient with abscesses and pain throughout his body for 1 mo was admitted to our hospital.Some of the abscesses had ruptured with purulent secretions on admission.Color Doppler ultrasound examination of the body surface masses showed mixed masses 75 mm×19 mm,58 mm×17 mm,17 mm×7 mm,and 33 mm×17 mm in size in the muscle tissues of both the right and left forearms,the posterior area of the right knee and the left leg,respectively.Abscess secretions and blood cultures grew B.gladioli.The following 3 methods were used to jointly identify the bacterium:an automatic microbial identification system,matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,and full-length 16 S rDNA sequencing.After 27 d of treatment with meropenem,etimicin,trimethoprim-sulfamethoxazole and other antibiotics,most of his skin abscesses were flat and he was discharged without any symptoms.CONCLUSION This is the first reported case of multiple skin abscesses associated with bacteremia caused by B.gladioli.Our study provides important reference values for the clinical diagnosis and treatment of B.gladioli infections.

关键词： Burkholderia gladioli Multiple skin abscesses Bacteremia Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Case report