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The Effect of Vineyard Cover Crop on Main Monomeric Phenols of Grape Berry and Wine in Vitis viniferal L. cv. Cabernet Sauvignon

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Agricultural Sciences in China 2010年第3期9卷 440-448页

作者： XI Zhu-mei ZHANG Zhen-wen cheng Yu-feng li Hua College of Enology Northwest A＆F University/Shaanxi Engineering Research Center for Viti-Viniculture Yangling 712100 P.R.China

This study was conducted to determine the effect of cover crop inter-row in vineyard on main mono-phenol content of grape berry and wine. Three such cover crops, two perennial legumes （white clover and alfalfa） and a perennial gramineous grass （tall fescue） were sown in vineyard. The main phenolic compounds of mature grape berry and wines vinified under the same conditions were extracted with ethyl acetate and diethyl ether and analyzed by high- performance liquid chromatography （HPLC） by comparing to soil tillage. A total of ten phenolic compounds were identified and quantified in the different grape berry and wines, including nonflavonoids （hydroxybenzoic and hydroxycinnamic acids） and flavonoids （flavanols and flavonols）. The concentration of flavonoid compounds （409.43 to 538.63 mg kg^-1 and 56.16 to 81.30 mg L^-1） was higher than nonflavonoids （76.91 to 98.85 mg kg^-1 and 30.65 to 41.22 mg L^-1） for Cabernet Sauvignon grape and wine under different treatments, respectively. In the flavonoid phenolics, Catechin was the most abundant in the different grapes and wines, accounting for 74.94 to 79.70% and 48.60 to 50.62% of total nonanthocyanin phenolics quantified, respectively. Compared to soil tillage, the sward treatments showed a higher content of main mono-phenol and total nonanthocyanin phenolics in grapes and wines. There were significant differences between two cover crop treatments （tall fescue and white clover） and soil tillage for the content of benzoic acid, salicylic acid, caffeic acid, catechin, and total phenolics in the grape berry （P 〈 0.05 or P〈0.01）. The wine from tall fescue cover crop had the highest gallic acid, caffeic acid and catechin. Cover crop system increased the total nonanthocyanin phenolics of grapes and wines in order of the four treatments： tall fescue, white clover, alfalfa, and soil tillage （control）. Cover crop in vineyard increased total phenols of grape berry and wine, and thus improved the quality of wine evidently.

关键词： vineyard cover crops inter-row Cabernet Sauvignon grape berry wine monomeric phenols

MiR-183-5p-PNPT1 Axis Enhances Cisplatin-induced Apoptosis in Bladder Cancer Cells

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Current Medical Science 2022年第4期42卷 785-796页

作者： Qing-gang HU Zhi YANG Jia-wei CHEN Gallina KAZOBINKA liang TIAN wen-cheng li Department of Urology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Hepatobiliary Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Urology Luoshan County People’s HospitalXinyang 464000China Urology Unit La Nouvelle Polyclinique Centrale de BujumburaBujumbura 378Burundi Department of Urology Wuhan Red Cross HospitalWuhan 430015China

Objective:It has been reported that intrinsic apoptosis is associated with the progression of bladder cancer(BC).Recent evidence suggests that polyribonucleotide nucleotidyltransferase 1(PNPT1)is a pivotal mediator involved in RNA decay and cell ***,the regulation and roles of PNPT1 in bladder cancer remain largely ***:The upstream miRNA regulators were predicted by in silico *** expression levels of PNPT1 were evaluated by real-time PCR,Western blotting,and immunohistochemistry(IHC),while miR-183-5p levels were evaluated by qPCR in BC cell lines and *** vitro and in vivo assays were performed to investigate the function of miR-183-5p and PNPT1 in apoptotic RNA decay and the tumorigenic capability of bladder cancer ***:PNPT1 expression was decreased in BC tissues and cell *** of PNPT1 significantly promoted cisplatin-induced intrinsic apoptosis of BC cells,whereas depletion of PNPT1 potently alleviated these ***,oncogenic miR183-5p directly targeted the 3′UTR of PNPT1 and reversed the tumor suppressive role of ***,miR-183-5p modulated not only PNPT1 but also Bcl2 modifying factor(BMF)to inhibit the mitochondrial outer membrane permeabilization(MOMP)in BC ***:Our results provide new insight into the mechanisms underlying intrinsic apoptosis in BC,suggesting that the miR-183-5p-PNPT1 regulatory axis regulates the apoptosis of BC cells and might represent a potential therapeutic avenue for the treatment of BC.

关键词： bladder cancer polyribonucleotide nucleotidyltransferase 1 bcl2 modifying factor mitochondrial outer membrane permeabilization microRNA

Cyclooxygenase-2 expression is dependent upon epidermal growth factor receptor expression or activation in androgen independent prostate cancer

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Asian Journal of Andrology 2008年第5期10卷 758-764页

作者： Rui-Peng Jia Lu-Wei Xu Qi Su Jian-Hua Zhao wen-cheng li Feng Wang Zheng Xu Department of Urology Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing 210006 China Department of Pathology Nanjing First Hospital Affiliated to NanjingMedical University Nanjing 210006 China Center Laboratory Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing 210006 China

Aim： To investigate the expression of cyclooxygenase-2 （COX-2） and epidermal growth factor receptor （EGFR） and the possible mechanism in the development in androgen independent prostate cancer （AIPC）. Methods： Immunohistochemistry was performed on paraffin-embedded sections with goat polyclonal against COX-2 and mouse monoclonal antibody against EGFR in 30 AIPC and 18 androgen dependent prostate cancer （ADPC） specimens. The effect of epidermal growth factor （EGF） treatments on the expression of COX-2 and signal pathway in PC-3 and DU-145 cells was studied using reverse transcription-polymerase chain reaction （RT-PCR） and Western blot analysis. EliSA was used to measure prostaglandin E2 （PGE2） levels in the media of PC-3 and DU-145 incubated with EGF for 24 h. Results： COX-2 was positively expressed in AIPC and ADPC, which were predominantly in endochylema of prostate cancer （PCa） cells. Intense staining was seen in AIPC （80%） and in ADPC （55.5%）, but there was no significant association between the two groups. EGFR expression was also positive in the two groups （61.8% in ADPC and 90% in AIPC, P 〈 0.01）. A significant association was found between EGFR expression and a higher Gleason score （P 〈 0.05） or tumor stage （P 〈 0.05）. The expression of PGE2 was increased in PC-3 and DU-145 cells after being incubated with EGF. Both p38MAPK and PI-3K pathway were involved in the PC-3 cell COX-2 upregulation course. In DU- 145, only p38MAPK pathway was associated with COX-2 upregulation. Conclusion： EGFR activation induces COX-2 expression through PI-3K and/or p38MAPK pathways. COX-2 and EGFR inhibitors might have a cooperative anti-tumor effect in PCa.

关键词： cyclooxygenase-2 epidermal growth factor receptor prostatic neoplasms

An idiopathic hypogonadotropic hypogonadism patient with metabolic disorder and diabetes： case report

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Asian Journal of Andrology 2015年第2期17卷 341-342页

作者： Man-Na Zhang Bin Su Chun-Hua Qian Le Bu Xiao-Yun cheng wen-Jun li Shen Qu Department of Endocrinology and Metabolism Shanghai Tenth People's HospitalTongji University School of Medicine 301 Middle Yanchang RoadShanghai 200072 China.

Dear Editor, Congenital idiopathic hypogonadotropic hypogonadism （CIHH） is a rare congenital disorder characterized by delayed or absent sexual maturation and infertility associated with inappropriately low gonadotropin and sex steroid levels. We report a 34-year-old patient with CIHH accompanying with metabolic syndrome （MS） and diabetes.

关键词： @@

Authors＇ Reply to Letter to the Editor ＂Comparing Sedation Regimens for Awake Fiberoptic Intubation＂

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Chinese Medical Journal 2016年第4期129卷 503-504页

作者： cheng-wen li Yan-Dong li Hai-Tao Tian Department of Anesthesiology Jining No. 1 People's Hospital Jining Shandong 272011 China Department of Anesthesiology Affiliated Hospital of Jining Medical University Jining Shandong 272029 China

We appreciate Prof. Xue et al. for their thoughtful comments on our studyJ H We agree with them that history of smoking and respiratory comorbidities can increase airway reactivity to airway irritation, resulting in an increased severity of cough. In our study, patients with a history of smoking and respiratory comorbidities were excluded and the demographic data of patients were comparable between tile groups.

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Dexmedetomidine-midazolam versus Sufentanil-midazolam for Awake Fiberoptic Nasotracheal Intubation： A Randomized Double-blind Study

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Chinese Medical Journal 2015年第23期128卷 3143-3148页

作者： cheng-wen li Yan-Dong li Hai-Tao Tian Xian-Gang Kong Kui Chen Department of Anesthesiology Jining No. 1 People's Hospital Jining Shandong 272011 China Department of Anesthesiology Affiliated Hospital of Jining Medical University Jining Shandong 272029 Chin

Background： Awake fiberoptic intubation （AFOI） is usually performed in the management of the predicted difficult airway. The aim of this study was to evaluate the feasibility of dexmedetomidine with midazolam （DM） and sufentanil with midazolam （SM） for sedation for awake fiberoptic nasotracheal intubation. Methods： Fifty patients with limited mouth opening scheduled for AFOI were randomly assigned to two groups （n = 25 per group） by a computer-generated randomization schedule. All subjects received midazolam 0.02 mg/kg as premedication and airway topical anesthesia with a modified ＂spray-as-you-go＂ technique. Group DM received dexmedetomidine at a loading dose of 0.5 μg/kg over 10 min followed by a continuous infllsion of 0.25 μ***^-1.h ^-1, whereas Group SM received sufentanil at a loading dose of 0.2 μg/kg over 10 rain followed by a continuous infusion of 0.1 μ***^-1.h ^-1. As necessary, since the end of the administration of the loading dose of the study drug, an additional dose ofmidazolam 0.5 mg at 2-min intervals was given to achieve a modified Observers＇Assessment of Alertness/Sedation of 2-3. The quality of intubation conditions and adverse events were observed.Results： The scores of ease of the AFOI procedure, patient＇s reaction during AFOI, coughing severity, tolerance after intubation, recall of the procedure and discomfort during the procedure were comparable in both groups （z = 0.572, 0.664, 1.297, 0.467, 0.895, and 0.188, respectively, P 〉 0.05）. Hypoxic episodes similarly occurred in the two groups, but the first partial pressure of end-tidal CO, after intubation was higher in Group SM than that in Group DM （45.2 ± 4.2 mmHg vs. 42.2 ±4.3 mmHg, t = 2.495, P 〈 0.05）. Conclusions： Both dexmedetomidine and sufentanil are effective as an adjuvant for AFOI under airway topical anesthesia combined with midazolam sedation, but respiratory depression is still a potential risk in the sufentanil regimen.

关键词： Awake Fiberoptic lntubation: Conscious Sedation: Dexmedetomidine Midazolam Sufentanil

Ultrasonography-guided Percutaneous Nephrolithotomy with Chinese One-shot Tract Dilation Technique Based on Stimulated Diuresis:A Report of 67 Cases

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2016年第6期36卷 881-886页

作者： Ying SHI Hua-geng liANG Xiong YANG Bo HAI liang WANG Yi-fei XING wen JU Fu-qing ZENG Xiao-ping ZHANG wen-cheng li Department of Urology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

The safety and effectiveness of a novel Chinese one-shot dilation technique based on stimulated diuresis for percutaneous nephrolithotomy（PCNL） were *** the feasibility of the Chinese one-shot dilation based on stimulated diuresis was verified by an animal study,this technique was applied in the clinical practice.A total of 67 patients in our department underwent the modified PCNL from July 2014 to June *** the renal infundibulum was distended by stimulated diuresis,the kidney was punctured under the ultrasonographic guidance via the fornix of the target *** working channel was dilated using a special designed pencil-shaped fascial *** successful access rate,nephrostomy tract creation time,pre-and postoperative hemoglobin values and serum creatinine concentrations,stone-free rate and complications were recorded and *** renal infundibulum was successfully distended in all of the patients by the diuresis *** the ultrasonographic guidance,the successful access rate was 100% and the mean tract creation time was 2.0 min（range：1.5–5.0 min）.The stone-free rate right after surgery was 91.0%.Although the postoperative hemoglobin was significantly reduced（P〈0.01）,transfusion was not clinically *** was no significant difference in serum creatinine concentrations before and after operation（P〉0.05）.No severe complication occurred during or after the *** was suggested that this Chinese one-shot dilation technique based on stimulated diuresis is an efficient and safe innovation for PCNL,and is even helpful for those patients with non-dilated pelvicaliceal systems.

关键词： percutaneous nephrolithotomy dilation stimulated diuresis ultrasonography

Real-world evidence on the efficacy and safety of vonoprazanamoxicillin dual therapy for Helicobacter pylori treatment in elderly patients

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World Journal of Gastroenterology 2025年第1期31卷 68-78页

作者： wen Gao Jing-wen li Hui Ye Xue-Zhi Zhang Jian-Xiang liu Hong cheng Department of Gastroenterology Peking University First HospitalBeijing 100034China School of Medicine Tsinghua MedicineTsinghua UniversityBeijing 100034China Department of Traditional Chinese Medicine and Integrative Medicine Peking University First HospitalBeijing 100034China

BACKGROUND A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of Helicobacter pylori(***).While substantial research supports the efficacy and safety of vonoprazan and amoxicillin(VA)dual therapy in the general population,there is still a lack of studies specifically focusing on its safety in elderly *** To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for *** in elderly *** As a real-world retrospective study,data were collected from elderly patients aged 60 years and above who accepted VA dual therapy(vonoprazan 20 mg twice daily+amoxicillin 1000 mg thrice daily for 14 days)for *** eradication in the Department of Gastroenterology at Peking University First Hospital between June 2020 and January *** status was evaluated by^(13)C-urease breath test 6 weeks after *** adverse events(AEs)during treatment were *** In total,401 cases were ***-one cases were excluded due to loss to follow-up,lack of re-examination,or unwillingness to take *** total of 380 included cases comprised 250 who received VA dual therapy as first-line treatment and 130 who received VA dual therapy as rescue *** was successfully eradicated in 239 cases(95.6%)in the first-line treatment group and 116 cases(89.2%)in the rescue treatment *** overall incidence of AEs was 9.5%for both ***,9.2%of patients experienced an AE in the first-line treatment group and 10.0%in the rescue treatment *** patients discontinued treatment due to AE,with a discontinuation rate of 1.3%.No serious AE *** The VA dual therapy regimen as a first-line treatment and a rescue therapy was effective and safe for elderly patients aged 60 and older.

关键词： Helicobacter pylori Vonoprazan Amoxicillin Dual therapy Elderly

Alprostadil liposome microsphere preparation stabilizes vascular plaques and inhibits intra-plaque inflammation

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Chinese Medical Journal 2012年第24期125卷 4380-4385页

作者： CHEN li cheng wen-li WANG Yong KE Yuan-nan FAN Shu-ying PAN lin GUO Yan-ru li Hong GUO Jian Department of National Integrative Medicine Center for Cardiovascular Disease China-Japan Friendship Hospital Beijing 100029 China Clinical Medical Research Institute China-Japan Friendship Hospital Beijing 100029 China

Background Vulnerable plaques play an important role in the onset of sudden cardiac events and strokes. How to stabilize vulnerable plaques is still a challenge to medical science. Alprostadil is a biologically active substance with strong activity on vessel. Our study assessed the stabilizing effects of an alprostadil liposome microsphere preparation （ALMP） on vulnerable plaques in the brachiocephalic artery of apolipoprotein E （Apo E） knockout mice. Methods Seventy-two male Apo E-knockout mice were fed a high-fat diet beginning at eight weeks of age. At week 17, they were divided randomly into groups for treatment with a high dose （3.6 μg, kg-1. d-1） or low dose （1.8 μg. kg-1 . d-1） of an ALMP, or 0.2 ml/d normal saline （control group）, The drug was administered using a micro-capsule pump. Twenty weeks after drug administration, pathological changes in the vulnerable plaques within the brachiocephalic artery were assessed, and levels of anti-mouse monocyte/macrophage monoclonal antibody （MOMA-2） and superoxide anions in the plaques were detected using immunofluorescence. The soluble intercellular adhesion molecule-1 （ICAM-1） expression was measured by EliSA, and the expression of matrix metalloproteinase-9 （MMP-9） and CD40 mRNA was measured using RT-PCR. Thrombospindin-1 （TSP-1） expression was detected using Western blotting. Results Compared with the control group, ALMP treatment significantly reduced the plaque area in the brachiocephalic artery （P 〈0.01）, significantly lowered the contents of the lipid core （P 〈0.01 ）, significantly reduced the number of ruptured fibrous caps （P 〈0.05）, and increased the thickness of the fibrous cap and significantly reduced the incidence of intra-plaque hemorrhage （P 〈0.05）. ALMP treatment significantly reduced the expression of MOMA-2, superoxide anion, MMP-9, ICAM-1 and CD40 in the plaques （P 〈0.01）, decreased plasma ICAM-1 expression （P 〈0.01 ）, and increased the expression of TSP-1. Conclusions Treatment with ALMP can stab

关键词： vulnerable plaque atherosclerosis alprostadil liposome microsphere preparation