检索结果-南通市图书馆

生物工程学报 2006年第5期22卷 829-834页

作者：王剑文郑丽屏谭仁祥苏州大学药学院苏州215123 苏州大学园艺系苏州215123 南京大学功能生物分子研究所南京210093

应用酸解法对黄花蒿(ArtemisiaannuaL.)内生胶孢炭疽菌(Colletotrichumgloeosporioides)菌丝体进行提取,在黄花蒿发根培养系统中比较了各制备提取物的青蒿素诱导活性。活性提取物经过SephadexG25层析后,部分纯化的内生菌寡糖提取物(MW<2... 详细信息

应用酸解法对黄花蒿(ArtemisiaannuaL.)内生胶孢炭疽菌(Colletotrichumgloeosporioides)菌丝体进行提取,在黄花蒿发根培养系统中比较了各制备提取物的青蒿素诱导活性。活性提取物经过SephadexG25层析后,部分纯化的内生菌寡糖提取物(MW<2500)可显著促进发根青蒿素的合成,培养23d的发根经诱导子(0.4mg/mL)处理4d后,青蒿素产量可达13.51mg/L,比同期对照产量提高51.63%,诱导作用与诱导子浓度、作用时间相关。内生菌寡糖诱导子的制备和使用,在青蒿素生物技术生产研究中为首次应用。

关键词：黄花蒿内生真菌胶孢炭疽菌诱导子青蒿素

Life Cycle of Morels-Fusion, Mating, Hybridization?

在线全文

学校读者我要写书评

暂无评论

Life Cycle of Morels-Fusion, Mating, Hybridization?

多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： He Xin-Sheng Zhao Miao Wang Ya-Zhou Zhang Neng Tan ren-Hao Wang Yin Zhu wen-Kun Chen Bo School of Life Science and Engineering Southwest University of Science and Technology

The hyphae of morels was cultured in flat plate, empty test tube and broken inclined medium. The fusion and non-fusion phenomenon between mycelia were observed in situ under the microscope. Including 9 species: Morchella sextelata, M. importuna, M. septimelata, Mel-21 and M. galilaea, Mes-16, Mes-19, Mes-20 and Mes-21. There are more than 2000 contact points between mycelia of more than 100 strains. After six years, mushroom production experiments were carried out with over 300 strains in more than 100 locations and over 500 hectares around the world. The results showed that the self-fusion of a few parts between the mycelial branches of the same monospora strain, and the fusion point did not produce new mycelia;the mycelia of the same species did not fuse, and the polyspore strain cultures of the same species did not fuse with each other;the mycelia of different species did not fuse;the incompatible mycelial contact points appeared: type II, type X, type Y, type T, typeπ, etc., the cells contacted but did not fuse;the sexual fusion between mycelia was not observed. The phenomena of death, winding, curling,knotting and swelling were observed after hyphal contact. The pure culture of morels mycelium could form asexual propagates such as conidiospores and sclerotium. The results of mushroom production test showed that the mushroom production rates of saprophytic Morchella species were 100% of more than 200 single-spore strains, 100% of more than 100 polyspores strains, less than 50% of more than 50 pure tissue isolates and less than 50% of more than 30 mixed *** were significant differences in morphology and yield of the mushroom producing strains,and none of the symbiotic Morchella strains produced mushrooms. The fruiting bodies of the same strain of mushroom-producing species showed morphological diversity after mushroom-producing. The fruiting bodies of different species after mixed sowing were the morphological characteristics of the original species. CO

关键词： Morchella hyphae Single-spore produce fruiting bodies Sexual fusion of hyphae Microscopic in situ direct observation Method of mycelium culture in empty test tube

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

基于IXP2400的安全网关实现研究

电子技术应用 2009年第7期35卷 142-144,148页

作者：文成玉任德浩文展成都信息工程学院四川成都610041

讨论了在IXP2400上应用IPSec实现安全网关的一种方案,可为通过的业务流提供较强的安全性。

关键词：网络处理器 IXP2400 微引擎 IPSec

Comparative transcriptome analyses on silk glands of six silkmoths imply the genetic basis of silk structure and coloration

在线全文

学校读者我要写书评

暂无评论

Comparative transcriptome analyses on silk glands of six sil...

第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会

作者： Yang Dong Fangyin Dai Yandong ren Hui Liu Lei Chen Pengcheng Yang Yanqun Liu Xin Li wen Wang Hui Xiang Kun ming University of Science and Technology State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences State Key Laboratory of Silkworm Genome Biology Key Sericultural Laboratory of Agricultural Ministry Institute of Sericulture and Systems Biology Southwest University Institute of Zoology Chinese Academy of Sciences Shenyang Agricultural University Center for Epigenetics John s Hopkins University School o f Medicine

Background: Silk has numerous unique properties that make it a staple of textile manufacturing for several thousand years. However, wider applications of silk in modern have been stalled due to limitations of traditional silk produced by Bombyx mori. While silk is commonly produced by B. mori, several wild non-mulberry silkmoths--especially members of family Saturniidae--produce silk with superior properties that may be useful for wider applications. Further utilization of such silks is hampered by the non-domestication status or limited culturing population of wild silkworms. To date there is insufficient basic genomic or transcriptomic data on these organisms or their silk production. Results: We sequenced and compared the transcriptomes of silk glands of six Saturniidae wild silkmoth species through next-generation sequencing technology, identifying 37758 51734 silkmoth unigenes, at least 36.3% of which are annotated with an e-value less than 10-5. Sequence analyses of these unigenes identified a batch of genes specific to Saturniidae that are enriched in growth and development. Analyses of silk proteins including fibroin and sericin indicate intra-genus conservation and inter-genus diversification of silk protein features among the wild silkmoths, e.g., isoelectric points, hydrophilicity profile and amino acid composition in motifs of silk H-fibroin. Interestingly, we identified p25 in two of the silkmoths, which were previously predicted to be absent in Saturniidae. There are rapid evolutionary changes in sericin proteins, which might account for the highly heterogeneity of sericin in Saturniidae silkmoths. Within the six sikmoths, both colored-cocoon silkmoth specific transcripts and differentially expressed genes between the colored-cocoon and non-colored-cocoon silkmoths are significantly enriched in catalytic activity, especially transferase activity, suggesting potentially viable targets for future gene mining or genetic manipulation. Conclusions: Our resu

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

深化医疗服务价格改革的背景、特点与思考

卫生经济研究 2021年第11期38卷 10-12,17页

作者：陈永成张雯雯任翔江西中医药大学经济与管理学院江西南昌330004 江西省医疗保障局江西南昌330046

《深化医疗服务价格改革试点方案》的审议通过,标志着我国医疗服务价格改革迈入一个新阶段。本文以医疗服务价格改革历程为切入点,通过文献调研和实地调查,探讨医疗服务价格改革的重点及挑战,并提出政策建议——加强与医保支付方式改革... 详细信息

《深化医疗服务价格改革试点方案》的审议通过,标志着我国医疗服务价格改革迈入一个新阶段。本文以医疗服务价格改革历程为切入点,通过文献调研和实地调查,探讨医疗服务价格改革的重点及挑战,并提出政策建议——加强与医保支付方式改革的协同、加快区块链医疗服务价格信息共享平台建设、构建一套有效的监测指标体系和综合评价方法。

关键词：医疗服务价格改革动态调整机制医保支付方式

在线全文

学校读者我要写书评

暂无评论

基于机器学习的高强度ODS合金成分设计

原子能科学技术 2020年第4期54卷 678-682页

作者：白冰郑全任帅张长义杨文胡长军中国原子能科学研究院反应堆工程技术研究部北京102413 北京科技大学北京100083

针对200~300组氧化物弥散强化(ODS)合金成分、工艺及力学性能数据,尝试借助机器学习的方法,建立了ODS合金中关键成分与拉伸性能的关联性。研究结果发现,在Cr、Y2O3、W和Ti含量与ODS合金抗拉强度的变化趋势中,均存在对应着抗拉强度极值... 详细信息

针对200~300组氧化物弥散强化(ODS)合金成分、工艺及力学性能数据,尝试借助机器学习的方法,建立了ODS合金中关键成分与拉伸性能的关联性。研究结果发现,在Cr、Y2O3、W和Ti含量与ODS合金抗拉强度的变化趋势中,均存在对应着抗拉强度极值的最优值,而添加Al对抗拉强度的提升无明显作用。获得了几种抗拉强度优化的ODS合金关键成分配比,预测出的室温抗拉强度均在1400 MPa以上,这将为快堆结构材料用ODS合金优化设计提供技术支持,加速推进ODS合金的材料优化。

关键词： ODS合金拉伸性能机器学习材料优化

Rapid detection of cAMP content in red jujube using near-infrared spectroscopy

在线全文

学校读者我要写书评

暂无评论

Optoelectronics Letters 2018年第5期14卷 380-383页

作者： YAN wen-li ren Shui-ying YUE Xia-xia TANG Jun CHEN Chen LU Xiao-yi MO Jia-qing School of lnformation Science and Engineering Xinjiang UniversityUrumqi 830046China Xinfiang Research Institute of Product Quality Supervision and Inspection Urumqi 830046China Physics and Chemistry Detecting Center Xinjiang UniversityUrumqi 830046China Institute of Health and Environmental Medicine Academy of Military Medical ScienceTianjin 300050China

In this paper, a new method for the rapid, economical and convenient detection of cyclic adenosine monophosphate （cAMP） in jujube is proposed and verified. Based on near-infrared （NIR） fiber spectroscopy combined with stoichi- ometric analysis, the cAMP content in red jujube can be quickly detected. 68 red jujube samples were used for the NIR spectroscopy data acquisition and the corresponding chemical values were determined. The sample set was adjusted based on the joint XY distance （SPXY） to select the correction sample set. After different preprocessing on the spectra, the partial least squares （PLS） method was used to establish the model, and the smoothed and normalized PLS model result was obtained better. The model＇s correction correlation coefficient （Re）, correction set mean square error （RMSEC）, prediction correlation coefficient （Rp）, and prediction and mean square error （RMsEe） are 0.95l5, 25.793 7, 0.910 8 and 28.228 0, respectively. The results show that NIR combined with specific chemometric methods can achieve rapid de- tection of cAMP in red iuiube.

关键词：光谱学红外线糖数据获得关联系数 NIR 均方差样品

绿色经济的内涵、演化逻辑及推进路径——基于经济——生态——社会复杂系统视角

在线全文

学校读者我要写书评

暂无评论

技术经济与管理研究 2020年第2期 88-93页

作者：任相伟孙丽文河北工业大学经济管理学院

能源危机和环境恶化倒逼绿色转型的背景下,绿色经济被纳入国家发展战略的范畴,并且已经进入经济—生态—社会复杂系统(E-E-S)阶段。针对当前将此复杂系统割裂研究,进而导致绿色经济演变逻辑轨迹不够清晰的问题,文章通过以下步骤对国内... 详细信息

能源危机和环境恶化倒逼绿色转型的背景下,绿色经济被纳入国家发展战略的范畴,并且已经进入经济—生态—社会复杂系统(E-E-S)阶段。针对当前将此复杂系统割裂研究,进而导致绿色经济演变逻辑轨迹不够清晰的问题,文章通过以下步骤对国内外相关文献进行梳理和研究。首先,对绿色经济在当前阶段的新内涵及内在协同演化机理进行了梳理和剖析,在原来以经济效率分析为主流视角的基础上,拓宽研究范围,并且将生态系统的核心自然资本纳入国家财富核算体系、社会系统的公平要素纳入发展框架,突破单一的生态治理;其次,厘清三大子系统的演化逻辑和相互作用机理,研究得出各子系统是交互影响、协同推进的关系,并且对社会系统公平要素起到调节经济效率和生态规模冲突的作用机理进行剖析。基于此,构建政策机制、技术创新和绿色投资三大因素为主的绿色转型动力模型,归纳绿色经济推进路径。

关键词：绿色经济 EES复杂系统绿色转型协同推进高质量发展

一种新的聚合物固载的1,3-二醇的合成及其对二醛的单保护性能

在线全文

学校读者我要写书评

暂无评论

化学学报 1989年第8期47卷 827-829页

作者：任启生黄文强何炳林南开大学高分子化学研究所

聚合物固载的1，2－二醇（1－3）和1，3－二醇（4，5）作为醛或酮的保护基团已有广泛的研究。聚合物固载的保护试剂的重要特性是能够对双功能基化合物进行单保护，上述二醇树脂用于对苯二甲醛单保护中只有在很低的醛固载量（0.2－0.38mm... 详细信息

聚合物固载的1，2－二醇（1－3）和1，3－二醇（4，5）作为醛或酮的保护基团已有广泛的研究。聚合物固载的保护试剂的重要特性是能够对双功能基化合物进行单保护，上述二醇树脂用于对苯二甲醛单保护中只有在很低的醛固载量（0.2－0.38mmol/g）时才是成功的，其单保护率可达95％以上。一旦固载的醛量增加，其单保护率迅速降低，而且这些二醇树脂多数不能再生重复使用，从这些二醇聚合物的结构分析可以看出，它们对二醛单保护率低的原因很可能是功能基通过醚键或酯键同聚合物相连，其柔性增加了二醛双结合的可能性。为了克服这些树脂的缺点，我们设计合成了二醇功能基直接与聚苯乙烯链的苯环相接的1，3－二醇树脂－－1－聚苯乙烯基－2，2－二甲基－1，3丙二醇（6），二醇功能基2位上的两个氢原子均被甲基所取代，减少了在被保护的醛解脱过程中二醇功能基脱水失活的可能性，以增加功能基的可再生性。

关键词：聚合物固载 1,3-二醇合成二醛保护基团单保护性能