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Enhancing Evolutionary Algorithms With Pattern Mining for Sparse Large-Scale Multi-Objective Optimization Problems

IEEE/CAA Journal of Automatica Sinica 2024年第8期11卷 1786-1801页

作者： Sheng Qi Rui Wang Tao Zhang weixiong huang Fan Yu Ling Wang College of Systems Engineering National University of Defense TechnologyChangsha 410073China College of Systems Engineering National University of Defense TechnologyChangsha 410073and also with the Xiangjiang LaboratoryChangsha 410205China College of Transportation and Engineering Central South UniversityChangsha 410073China Department of Automation Tsinghua UniversityBeijing 100084China

Sparse large-scale multi-objective optimization problems(SLMOPs)are common in science and ***,the large-scale problem represents the high dimensionality of the decision space,requiring algorithms to traverse vast expanse with limited computational ***,in the context of sparse,most variables in Pareto optimal solutions are zero,making it difficult for algorithms to identify non-zero variables *** paper is dedicated to addressing the challenges posed by *** start,we introduce innovative objective functions customized to mine maximum and minimum candidate *** substantial enhancement dramatically improves the efficacy of frequent pattern *** this way,selecting candidate sets is no longer based on the quantity of nonzero variables they contain but on a higher proportion of nonzero variables within specific ***,we unveil a novel approach to association rule mining,which delves into the intricate relationships between non-zero *** novel methodology aids in identifying sparse distributions that can potentially expedite reductions in the objective function *** extensively tested our algorithm across eight benchmark problems and four real-world *** results demonstrate that our approach achieves competitive solutions across various challenges.

关键词： Evolutionary algorithms pattern mining sparse large-scale multi-objective problems(SLMOPs) sparse large-scale optimization.

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Oomycete Pathogen Pectin Acetylesterase Targets Host Lipid Transfer Protein to Reduce Salicylic Acid Signaling

Oomycete Pathogen Pectin Acetylesterase Targets Host Lipid T...

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中国植物病理学会2024年学术年会

作者： Junjian Situ SONG Yu FENG Dinan WAN Lang LI Wen NING Yue huang weixiong LI Minhui XI Pinggen DENG Yizhen JIANG Zide KONG Guanghui National Key Laboratory of Green Pesticide/Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Integrative Microbiology Research Center South China Agricultural University

During initial stages of microbial invasion,the extracellular space(apoplast) of plant cells is a vital battleground between plants and *** oomycete plant pathogens secrete an array of apoplastic carbohydrate active enzymes,which are central molecules for understanding the complex plant-oomycete *** them,pectin acetylesterase(PAE) plays a critical role in the pathogenesis of plant pathogens including bacteria,fungi,and ***,we demonstrated that Peronophythora litchii(*** litchii) PlPAE5 suppresses litchi(Litchi chinensis) plant immunity by interacting with litchi lipid transfer protein 1(LcLTP1).The LcLTP1-binding activity and virulence function of PlPAE5 depend on its PAE domain but not on its PAE *** high expression of LcLTP 1 enhances plant resistance to oomycete and fungal pathogens,and this disease resistance depends on BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1(BAK1) and Suppressor of BIR1(SOBIR1) in Nicotiana *** 1 activates the plant salicylicacid(SA) signaling pathway,while PlPAE5 subverts the LcLTP1-mediated SA signaling pathway by destabilizing ***,this study reports a virulence mechanism of oomycete PAE suppressing plant LTP-mediated SA immune signaling and will be instrumental for boosting plant resistance breeding.

关键词： oomycete LTP-mediated SA virulence mechanism

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Characterization of full-length transcriptome and mechanisms ofsugar accumulation in Annona squamosa fruit

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BIOCELL 2020年第4期44卷 737-750页

作者： REN FANG weixiong huang JINYAN YAO XING LONG JI ZHANG ShuangYUN ZHOU BIAO DENG WENZHONG TANG ZHENYU AN Horticultural Research Institute Guangxi Academy of Agricultural SciencesNanning530007China

Annona squamosa is a multipurpose fruit tree employed in nutritional,medicinal,and industrial *** fruit is significantly enriched in sugars,making it an excellent species to study sugar accumulation in ***,the scarcity of genomic resources hinders genetic studies in this *** study aimed at generating large-scale genomic resources in *** and deciphering the molecular basis of its high sugar ***,we sequenced and characterized the full-length transcriptome of *** fruit using PacBio *** addition,we analyzed the changes in sugar content over five fruit growth and ripening stages,and we applied RNA-sequencing technology to investigate the changes in gene expression related to sugar accumulation.A total of 783,647 circular consensus sequences were generated,from which we obtained 48,209 high-quality,full-length ***,1,838 transcription factors and 1,768 long non-coding RNAs were ***,we identified 10,400 alternative splicing events from 2,541 unigenes having on average 2–4 isoforms.A total of 15,061 simple sequence repeat(SSR)motifs were discovered and up to three primer pairs were designed for each SSR *** mainly accumulate during the ripening stage in *** of the genes involved in sugar transport and metabolism in the fruit were progressively repressed overgrowth and ripening ***,sucrose phosphate synthase involved in sucrose synthesis and more importantly,isoamylase,alpha-amylase,beta-amylase,4-alphaglucanotransferase genes involved in starch degradation displayed positive correlations with sugar accumulation in ***,we provide here a high-quality,full-length transcriptome assembly which will facilitate gene discovery and molecular breeding of *** found that starch degradation during fruit ripening was the main channel for sugar accumulation in *** fruit,and the key genes positively linked to sugar accumulation could be f

关键词： PacBio sequencing Sugar metabolism Gene expression Alternative splicing Simple sequence repeat

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大豆膜下表层内环流通风技术研究

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粮食储藏 2024年第4期53卷 52-56页

作者：李展雄杨富东农世康黄伟雄唐斌欧兴宇黄安昭黄浙文防城港中储粮仓储有限公司 538001 中央储备粮海口直属库有限公司 571152

广西地处高温高湿第七储粮生态区,受气候因素影响,内环流技术应用普遍性和成熟性与北方存在一定差距。结合本区储粮特性及公司多年来的大豆储藏情况,通过对充氮气调情况下膜下粮堆表层内环流通风技术的研究,以解决大豆生霉及快速杀虫问... 详细信息

广西地处高温高湿第七储粮生态区,受气候因素影响,内环流技术应用普遍性和成熟性与北方存在一定差距。结合本区储粮特性及公司多年来的大豆储藏情况,通过对充氮气调情况下膜下粮堆表层内环流通风技术的研究,以解决大豆生霉及快速杀虫问题,确保储粮安全。结果表明,该技术可以有效降低粮堆表面墙壁及四周空间气体湿度,减少水分增加及快速均匀粮堆内氮气浓度,从而达到减少发霉等异常粮情、快速有效杀虫、减少储粮安全隐患和降低储粮成本的目的。

关键词：大豆膜下内环流生霉杀虫第七储粮生态区

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宜昌地区某中低品位磷矿光电选矿工艺试验研究

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资源环境与工程 2024年第4期38卷 457-462,471页

作者：孙唯衡黄伟雄李亮吴鹏董国亮曾靖钊郭安湖北省海外地质事业中心湖北武汉430034 中海油化学宜昌矿业有限公司湖北宜昌443145

为探索光电选矿工艺在宜昌某中低品位磷矿的适用性,分别对该磷矿Ph_(1)^(3)下磷层和Ph_(2)^(2)中磷层中低品位条带状矿石开展工艺矿物学研究,查明其化学组成、磷元素分布、矿物组成、嵌布关系等特征,然后开展光电选矿试验研究。结果表明... 详细信息

为探索光电选矿工艺在宜昌某中低品位磷矿的适用性,分别对该磷矿Ph_(1)^(3)下磷层和Ph_(2)^(2)中磷层中低品位条带状矿石开展工艺矿物学研究,查明其化学组成、磷元素分布、矿物组成、嵌布关系等特征,然后开展光电选矿试验研究。结果表明,磷元素主要分布在胶磷矿条带中,硅酸盐或碳酸盐等脉石条带中含磷较低,条带界线截然,宽度一般为0.5~3 cm,此矿石特性非常有利于通过光电选矿作业抛除废石实现预富集。将硅酸盐条带型和碳酸盐条带型磷矿石分别破碎筛分预处理后,利用XRT/CCD双融合智能光电选矿机对8~28 mm粒径组分进行选别试验,在抛废率为45%左右时,预处理粉矿(1.2,综合回收率>85%,抛废效果明显,选别指标良好。该研究证实光选工艺可满足矿山中低品位磷矿石选矿需求,可为矿山后期选矿工艺方案选择提供一定的指导。

关键词：中低品磷矿工艺矿物学光电选矿 XRT CCD

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Recent progress in co-detection of single-cell transcripts and proteins

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Nano Research 2024年第1期17卷 132-150页

作者： Shanqing huang Qian Fan Yidi Wang Zhi huang weixiong Shi Yanli Gong Ting Yang Jie Wang Lingling Wu Chaoyong Yang Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of General Surgery Xiang’an Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361101China Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China

Cellular heterogeneity is a universal property of living systems,and the interrogation of single cells facilitates in-depth understanding of distinct cellular states and functions in various biological ***-analysis of transcripts and proteins from the same single cells opens the way to decipher complex RNA regulatory frameworks and phenotypes,facilitating the understanding of cellular fate and function regulations,discovery of novel cell types,and construction of a high-resolution cell ***,we review the state-of-art advances in the development of methodologies for co-analysis of single-cell transcripts and ***,imaging-based methods are summarized with particular emphasis on optical and mass spectrometry ***,sequencing-based approaches for high-throughput and sensitive co-analysis of single-cell transcripts and proteins are described,including droplet-,microwell-,and split-pool-based ***,combined methods with more flexibility and universality are *** methods commonly employ different strategies or reactions to convert transcripts and proteins of single cells into distinct signals simultaneously,which can be detected by different instruments or ***,some perspectives on the future challenges and development trends in this field are presented.

关键词： single cell optical imaging mass spectrometry imaging single-cell RNA sequencing(scRNA-seq) co-detection

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开管离子色谱柱的制备与表征

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色谱 2020年第4期38卷 399-408页

作者：黄维雄中国地质大学(武汉)环境学院

近年来,开管离子色谱(OTIC)的研究已取得重大进展,尤其是兼容OH^-淋洗液的OTIC柱的成功研制使OTIC梯度洗脱得以实现。该文介绍了各种OTIC柱的制备和表征方法,涵盖早期的二氧化硅开管柱和新近出现的有机聚合物基质开管柱,对静电乳胶附聚... 详细信息

近年来,开管离子色谱(OTIC)的研究已取得重大进展,尤其是兼容OH^-淋洗液的OTIC柱的成功研制使OTIC梯度洗脱得以实现。该文介绍了各种OTIC柱的制备和表征方法,涵盖早期的二氧化硅开管柱和新近出现的有机聚合物基质开管柱,对静电乳胶附聚的OTIC柱的一系列表征方法进行了系统阐述,包括开管柱柱容量的实验测定和理论计算方法、固定相相同时填充柱与开管柱之间的联系、柱效改善方法以及开管柱均一性表征等。

关键词：开管柱制备毛细管柱乳胶附聚离子色谱综述

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Multi-omics Approach Reveals Influenza-A Virus Target Genes Associated Genomic,Clinical and Immunological Characteristics in Cancers

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Biomedical and Environmental Sciences 2024年第7期37卷 698-715页

作者： Jiaojiao Wang Yong Liao Pinglian Yang Weile Ye Yong Liu Chunxia Xiao weixiong Liao Chunbo Chen Zhiping Liu Zunnan huang Center of Scientific Research Department of CardiologyMaoming People’s HospitalMaoming 525000GuangdongChina Department of Pharmacy Maoming People’s HospitalMaoming 525000GuangdongChina State Key Laboratory of Bioactive Molecules and Druggability Assessment Jinan UniversityGuangzhou 510632GuangdongChina Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research College of PharmacyJinan UniversityGuangzhou 510632GuangdongChina Key Laboratory of Big Data Mining and Precision Drug Design of Guangdong Medical University Key Laboratory of Computer-Aided Drug Design of Dongguan CityKey Laboratory for Research and Development of Natural Drugs of Guangdong ProvinceSchool of PharmacyGuangdong Medical UniversityDongguan 523808GuangdongChina Department of Critical Care Medicine Maoming People’s HospitalMaoming 525000GuangdongChina

Objective To examine the precise function of influenza A virus target genes(IATGs)in *** Using multi-omics data from the TCGA and TCPA datasets,33 tumor types were evaluated for *** expression in cancer cells was analyzed using transcriptome *** number variation(CNV)was assessed using GISTICS ***’s analysis was used to correlate mRNA expression with methylation *** was used for the enrichment ***’s correlation analysis was used to examine the association between IATG mRNA expression and *** ImmuCellAI algorithm was used to calculate the infiltration scores of 24 immune cell *** In 13 solid tumors,IATG mRNA levels were atypically *** for UCS,UVM,KICH,PCPG,THCA,CHOL,LAMI,and MESO,most cancers contained somatic IATG *** main types of CNVs in IATGs are heterozygous amplifications and *** most tumors,IATG mRNA expression is adversely associated with ***-PCR demonstrated that EGFR,ANXA5,CACNA1C,CD209,UVRAG were upregulated and CLEC4M was downregulated in KIRC cell lines,consistent with the TCGA and GTEx *** Genomic changes and clinical characteristics of IATGs were identified,which may offer fresh perspectives linking the influenza A virus to cancer.

关键词： Genomic changes Immune microenvironment Prognosis Drug resistance Experimental validation

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“吉夫纳”番荔枝生物学特性及栽培技术

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广西农学报 2024年第3期39卷 148-152页

作者：劳素婵安振宇黄伟雄潘贞珍李健琪周春衡陈科文方仁广西硕果农业开发有限公司广西南宁532709 广西壮族自治区农业科学院园艺研究所广西南宁530007 广西南亚热带农业科学研究所广西崇左532406 云南省玉溪市元江哈尼族彝族傣族自治县农业广播电视学校云南玉溪653300 广西迦迦乐农业开发有限公司广西南宁532707

“吉夫纳”作为当前广西番荔枝产业发展的主要品种,其种植效益尤为突出。通过多年观察该品种的植物学特性、生长结果习性、果实经济性状和物候期等特性,发现其在广西种植区的综合表现优良,具体表现为:早结,定植2年即可投产;丰产,每667 m... 详细信息

“吉夫纳”作为当前广西番荔枝产业发展的主要品种,其种植效益尤为突出。通过多年观察该品种的植物学特性、生长结果习性、果实经济性状和物候期等特性,发现其在广西种植区的综合表现优良,具体表现为:早结,定植2年即可投产;丰产,每667 m2平均产量可达1151.7 kg;优质,可溶性固形物含量为21.68%、维生素C含量为32.40 mg/100 g、K含量为284.82 mg/100 g、Ca含量为25.02 mg/100 g;产期易调节,4月~10月修剪即可成花。并探索总结出该品种在高标准建园、强化水肥管理、科学产期调节、精细花果管理和综合防控病虫害等栽培领域上的技术要点,以期为“吉夫纳”在广西适栽区的推广应用提供理论参考。

关键词： “吉夫纳”番荔枝生物学特性栽培技术

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广东经济鱼类不饱和脂肪酸的含量分析与组成特征评价

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环境化学 2020年第5期39卷 1181-1191页

作者：张文凤黄伟雄中国广州分析测试中心广东省测试分析研究所广东省食品营养与安全快速检测仪器工程技术研究中心广州510070 广东省疾病预防控制中心国家食品安全风险监测参比实验室(重金属)广州511430

采用水解提取-气相色谱法测定鱼肉中脂肪酸的含量和组成,采用元素分析仪-同位素比值质谱法(EA-IRMS)测定鱼肉碳稳定同位素比值(δ13C).开展广东沿海地区主要经济鱼类肌肉脂肪酸的含量分析及组成特征评价研究.研究表明,25种经济鱼类鱼肉... 详细信息

采用水解提取-气相色谱法测定鱼肉中脂肪酸的含量和组成,采用元素分析仪-同位素比值质谱法(EA-IRMS)测定鱼肉碳稳定同位素比值(δ13C).开展广东沿海地区主要经济鱼类肌肉脂肪酸的含量分析及组成特征评价研究.研究表明,25种经济鱼类鱼肉中主要含有单不饱和脂肪酸、n-6和n-3系列多不饱和脂肪酸,以及少量反式脂肪酸(TFAs);鱼肉的优势脂肪酸为油酸(OA)、亚油酸(LA)和二十二碳六烯酸(DHA);鲻鱼含有较高的二十碳五烯酸(EPA)和DHA,合计370.0 mg·(100 g)^-1;鲻鱼鱼肉中TFAs的含量(280.0 mg·(100 g)^-1)远高于其它品种鱼类;鲢鱼和黑鲷EPA+DHA的含量较高,分别为(154.4±100.0 mg·(100 g)^-1)和1287±291.1 mg·(100 g)^-1,两种鱼均具有较好的商业开发价值;统计学分析结果表明了鱼类的不饱和脂肪酸在组成上具有种属差异,揭示了浮游食性的鱼类其鱼肉组织中MUFAs、n-6 PUFAs以及n-3 PUFAs的含量高于底栖食性的鱼类.鱼肉脂肪酸的组成差异较大,表现为品种、空间以及食性差异.建议市民在日常膳食中丰富食鱼的种类和数量,警惕过多地食用单一品种鱼类(尤其是鲻鱼)而导致TFAs摄入过多的风险,从而确保鱼类脂肪酸的平衡摄入.

关键词：不饱和脂肪酸组成特征健康风险经济鱼类差异分析

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