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Expression of hypoxia-inducible factor 1 alpha and oligodendrocyte lineage gene-1 in cultured brain slices after oxygen-glucose deprivation

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Neural Regeneration Research 2013年第4期8卷 328-337页

作者： Hong Cui weijuan han Lijun Yang Yanzhong Chang College of Life Science Hebei Normal University Department of Pediatrics Beijing Friendship HospitalCapital Medical University

Oligodendrocyte lineage gene-1 expressed in oligodendrocytes may trigger the repair of neuronal myelin impairment, and play a crucial role in myelin repair. Hypoxia-inducible factor la, a transcription factor, is of great significance in premature infants with hypoxic-ischemic brain damage There is little evidence of direct regulatory effects of hypoxia-inducible factor le on oligodendrocyte lineage gene-l. In this study, brain slices of Sprague-Dawley rats were cultured and subjected to oxygen-glucose deprivation. Then, slices were transfected with hypoxia-inducible factor la or oligodendrocyte lineage gene-1. The expression levels of hypoxia-inducible factor la and oligodendrocyte lineage gene-1 were significantly up-regulated in rat brains prior to transfection, as detected by immunohistochemical staining. Eight hours after transfection of slices with hypoxia-inducible factor la, oligodendrocyte lineage gene-1 expression was upregulated, and reached a peak 24 hours after transfection. Oligodendrocyte lineage gene-1 transfection induced no significant differences in hypoxia-inducible factor la levels in rat brain tissues with oxygen-glucose deprivation. These experimental findings indicate that hypoxia-inducible factor la can regulate oligodendrocyte lineage gene-1 expression in hypoxic brain tissue, thus repairing the neural impairment.

关键词： neural regeneration brain injury biological factors hypoxia-inducible factor la oligodendrocyte lineage gene-1 oxygen-glucose deprivation brain slice culture immunohistochemistry oligodendrocyte myelin repair premature delivery rat grants-supported paper photographs-containing paper neuroregeneration

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Oligodendrocyte transcription factor 1 mRNA and protein expression in organotypic rat brain slices

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Neural Regeneration Research 2010年第21期5卷 1639-1643页

作者： Hong Cui Lijun Yang Dezhuang Huang Wandong Zhang weijuan han Yanqing Yao Wenxing Jiang Pediatrics Department Beijing Friendship Hospital Capital Medical University Beijing 100050 China Hepatitis Institute Beijing YouAn Hospital Capital Medical University Beijing 100054 China Institute for Biological Sciences National Research Council of Canada Ottawa K1A OR6 Ontario Canada

Numerous studies have confirmed that oligodendrocyte transcription factor 1 （Olig-1） is vital for myelin repair. However, the effects of hypoxia and ischemia on Olig-1 expression remain unknown. In this study, Olig-1 mRNA and protein expressions were analyzed by in situ hybridization and immunohistochemistry, to determine the expression profile of Olig-1 in rat brain slices exposed to hypoxia and ischemia. Brains were obtained from 2-day-old Sprague-Dawley rats, and sections were randomly assigned to control and hypoxia/ischemia groups. Hematoxylin-eosin staining revealed karyorrhexis and karyopyknosis in cells from the hypoxia/ischemia group. Under electron microscopy, mitochondria swelling and neuropil edema were observed in the hypoxiaJischemia group. Olig-1 mRNA and protein expressions were increased at 1 day after hypoxia and ischemia treatment. These results suggest that in situ hybridization and immunohistochemistry could be used simultaneously to detect mRNA and protein expression in brain slices.

关键词： oligodendrocyte transcription factor 1 double staining brain slices immunohistochemistry in situ hybridization hypoxia and ischemia

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Expression and regulation of HIF-1α/Olig-1 in neonatal rats after hypoxic ischemic brain damage

Expression and regulation of HIF-1α/Olig-1 in neonatal rats...

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第二十三次全国儿科中西医结合学术会议

作者： Lijun Yang Yingxue Ding weijuan han Nan Zhang Hong Cui Beijing Friendship Hospital Capital Medical UniversityPediatrics

Objective: To investigate the expression and interaction of HIF-1α and Olig-1 in neonatal rats after hypoxic ischemic brain damage(HIBD). Methods: To generate a model of HIBD, neonatal rats were randomly divided into a control group, a hypoxic ischemic group and a transfection group. The transfection group was transfected with either HIF-1α or Olig-1 using an adenoviral vector. Both mRNA and protein levels were detected at different time points. An electrophoretic mobility shift assay(EMSA) was employed to determine whether there was mutual regulation between HIF-1α and Olig-1. Microstructural changes to myelin, synapses, and axons were observed under an electron microscope. A water maze test was used to evaluate the learning and memory ability of rats at post-natal day(P) 28. Results: HIF-1α mRNA reached peak levels in neonatal rats at 24 h post HIBD, followed by a significant decrease at day 3, although still significantly increased compared to the control group. After day 3, HIF-1α mRNA was kept at low expression levels. The expression trend of HIF-1α mRNA in the transfection groups was similar to the control group. There were two peaks in the expression levels of Olig-1 mRNA following HIBD;Olig-1 mRNA was significantly increased in the group transfected with HIF-1α, displaying a second expression peak on day 7 post-HIBD. Olig-1 transfection did not affect the mR NA levels of Olig-1. The protein levels of HIF-1α and Olig-1 were increased at day 14 post HIBD relative to the control group. EMSA data showed that HIF-1α bound to Olig-1. A water maze test conducted at P28 revealed that the maze escape latency was significantly increased in the HIBD group, and decreased in rats transfected with Olig-1 or HIF-1a, compared to rats in the HIBD group. The rates of nuclear pyknosis and fragmentation were decreased, and the neuronal viability and synapse numbers were increased, in rats that received HIF-1α/Olig-1 transfection. Conclusion: HIF-1α and Olig-1 expression are upregulated after HIBD in neonatal rats, with regul

关键词： hypoxia ischemia oligodendrocyte transcription factor 1,hypoxia-inducible factor 1 α

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论温儒敏以“母语教育观”为核心的语文教育思想

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天津师范大学学报（社会科学版） 2023年第4期 89-95页

作者：韩卫娟北京联合大学师范学院北京100101

温儒敏将语文教育视为“母语教育”,在继承百年来母语教育良好经验的基础上,遵从母语教育的实际和基本规律,充分肯定了语文学科的基础地位和独特价值。“阅读教学观”“课程改革观”“高考作文观”是温儒敏对母语教育思考的三个方面。... 详细信息

温儒敏将语文教育视为“母语教育”,在继承百年来母语教育良好经验的基础上,遵从母语教育的实际和基本规律,充分肯定了语文学科的基础地位和独特价值。“阅读教学观”“课程改革观”“高考作文观”是温儒敏对母语教育思考的三个方面。“阅读教学观”发展了阅读教学史上零散的“精读”“略读”“课外阅读”思想,建构了“教读—自读—课外阅读”三位一体的理念,将“读书为要”落到实处。“课程改革观”致力于规避语文课改中盲目追求人文性、任务驱动的倾向,提倡语言文字听说读写基本能力的培养,保证大多数学生需要的“基本口粮”。在“高考作文观”方面,温儒敏则主张从高考作文命题到平时的作文训练,都应该注重学生谋篇布局时理性精神和逻辑思辨能力的培养,抵制泛文学化和片面强调创意能力、修辞技巧的文风。

关键词：温儒敏母语教育观阅读教学观课程改革观高考作文观

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Maps Preserving Semi-Fredholm Operators with Fixed Nullity

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Journal of Mathematical Research with Applications 2024年第4期 533-540页

作者： Mengran NI weijuan SHI Department of Mathematics Shaanxi Normal University

Let X be an infinite-dimensional complex Banach space, and B(X) the algebra of all bounded linear operators on X. Given an integer n ≥ 1, we characterize all the bijective maps on B(X) preserving the difference of semi-Fredholm operators with nullity equal to n in both directions, and establish the structure of the given maps.

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Seismic-induced surficial failure of cohesive slopes using three-dimensional limit analysis:A case study of the Wangjiayan landslide in Beichuan, China

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Earthquake Engineering and Engineering Vibration 2024年第3期23卷 537-545页

作者： Gao Yufeng Liu Yang Geng weijuan Zhang Fei Key Laboratory of the Ministry of Education for Geomechanics and Embankment Engineering College of Civil and Transportation EngineeringHohai UniversityNanjing 210098China Department of Civil Engineering Faculty of Civil Engineering and MechanicsJiangsu UniversityZhenjiang 212013China

A seismic-induced landslide is a common geological catastrophe that occurs in nature.The Wangjiayan landslide,which was triggered by the Wenchuan earthquake,is a typical case in point.The Wanjiayan landslide caused many casualties and resulted in enormous property loss.This study constructs a simple surficial failure model based on the upper bound approach of three-dimensional(3D)limit analysis to evaluate the slope stability of the Wangjiayan case,while a traditional two-dimensional(2D)analysis is also conducted as a reference for comparison with the results of the 3D analysis.A quasi-static calculation is used to study the effect of the earthquake in terms of horizontal ground acceleration,while a parametric study is conducted to evaluate the critical cohesion of slopes.Rather than employing a 3D analysis,using the 2D analysis yields an underestimation regarding the safety factor.In the Wangjiayan landslide,the difference in the factors of safety between the 3D and 2D analyses can reach 20%.The sliding surface morphology as determined by the 3D method is similar to actual morphology,and the parameters of both are also compared to analyze the reliability of the proposed 3D method.

关键词： landslide Wenchuan earthquake surficial failure limit analysis stability quasi-static

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1904年—1935年的语文教育变革与五四新文学的互动

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天津师范大学学报（社会科学版） 2020年第4期40卷 70-75页

作者：韩卫娟北京联合大学师范学院北京100101 上海师范大学教育学院博士后流动站

1904年到1935年的语文教育变革与五四新文学关系密切。二者的互动体现于三个层面,一是现代语文教育变革之初即以"能自由发表思想"为目标,与五四"人的文学"理念相辅相成;二是语文教科书内容由文言向白话倾斜,新文学作品大量入选,加快了... 详细信息

1904年到1935年的语文教育变革与五四新文学关系密切。二者的互动体现于三个层面,一是现代语文教育变革之初即以"能自由发表思想"为目标,与五四"人的文学"理念相辅相成;二是语文教科书内容由文言向白话倾斜,新文学作品大量入选,加快了新文学的普及进度,教科书可以视为新文学经典建构过程中最重要的选本;三是教学方法上尊重学生的主动性、重视儿童的视角,此为五四人文精神在教育中的落实。这一时期的经验规划、思路观点,对于现在的语文教育改革,仍具有重要的参照价值。

关键词：语文教育变革五四新文学课程目标课程内容人的文学

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在一项宏大的系统工程之中--试论金宏宇教授的版本批评和副文本研究

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晋阳学刊 2020年第5期 59-67页

作者：韩卫娟北京联合大学师范学院北京100101

史料建设工作是中国现代文学学科20世纪80年代再出发后成果最为丰硕的部分,但该研究也面临着方法论层面的发展和调试:如何承继此前朴学研究的方法和精神,同时在西方文学资料问题的理论考察中吸纳一切有益的成果,拓展研究的视野,丰富考... 详细信息

史料建设工作是中国现代文学学科20世纪80年代再出发后成果最为丰硕的部分,但该研究也面临着方法论层面的发展和调试:如何承继此前朴学研究的方法和精神,同时在西方文学资料问题的理论考察中吸纳一切有益的成果,拓展研究的视野,丰富考察的手段,从根本上完成现代文学史料工作的现代化转型,这是当下急迫且具有重大理论价值的问题。在这一方面,金宏宇教授进行了非常有益的探索,他所倡导的“版本批评”和“副文本研究”,对于史料研究范式的转变,具有关键性的推动之效。

关键词：现代文学史料版本批评副文本研究学科史

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纬编单珠地网眼毛圈面料编织工艺设计

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针织工业 2022年第4期 9-12页

作者：徐伟许玉红郎苹韩伟娟宁波大千纺织品有限公司浙江宁波315800

针对市面上经典的纬编毛圈结构往往要求专用机器编织、结构花型变化困难的问题,利用针织厂常见的机型,将衬垫组织、添纱衬垫组织与经典的单珠地网眼结合,设计开发3款大圆机单珠地网眼毛圈面料。详细介绍面料的编织工艺参数、织针三角排... 详细信息

针对市面上经典的纬编毛圈结构往往要求专用机器编织、结构花型变化困难的问题,利用针织厂常见的机型,将衬垫组织、添纱衬垫组织与经典的单珠地网眼结合,设计开发3款大圆机单珠地网眼毛圈面料。详细介绍面料的编织工艺参数、织针三角排列图、线圈图和面料实物图等,并对纬编单珠地网眼毛圈面料的特点、设计原理进行阐述。毛圈结构与网眼结构的结合拓宽了普通大圆机的生产能力,丰富了纬编毛圈产品的多样性。

关键词：单珠地网眼毛圈布衬垫组织添纱衬垫组织编织工艺成圈系统

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Sanhua Tang(三化汤) protects against ischemic stroke by preventing blood-brain barrier injury: a network pharmacology and in vivo experiments

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Journal of Traditional Chinese Medicine 2024年第4期 794-803页

作者： LUO Shan YANG Fan CHEN Yuanchun ZHAO Ruoxi LIU Haiye GAO Fei MA Wencan GAO weijuan YU Wentao College of Basic Medicine Hebei University of Chinese Medicine Graduate School Hebei University of Chinese Medicine College of Acupuncture and Massage Hebei University of Chinese Medicine Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Diseases Hebei International Joint Research Centre for Acupuncture and Moxibustion of Traditional Chinese Medicine

OBJECTIVE: To assess the effect and mechanism of Sanhua Tang(三化汤, SHT) in treating ischemic stroke(IS) through the "Kaitong Xuanfu" theory by using network pharmacology and animal experiments. METHODS: The active ingredients and targets of SHT and IS were screened by public databases such as Traditional Chinese Medicine systems pharmacology, GeneCards, and online mendelian inheritance in man. Visual network topographies were constructed using R, Cytoscape 3.6.0, AutoDockTools, a user-sponsored molecular visualization system on an open-source foundation, and other software to analyze the correlation between targets and active ingredients. The middle cerebral artery occlusion(MCAO) model was established by operation. Animals were divided into the Sham group, MCAO group(M group), aloe-emodin(AE) group(MCAO rats treated with aloe-emodin), SHT at low dosage(SL group)(MCAO rats treated with SL), SHT at medium dosage(SM group), and SHT at high dosage(SH group). 2,3,5-triphenyl tetrazolium chloride staining was used to detect the volume of cerebral infarction; Nissl staining was used to observe the morphology of neuronal cells; transmission electron microscopy was used to observe the integrity of the blood-brain barrier(BBB); enzymelinked immunosorbent assay was used to detect the content of interleukin-6(IL-6), IL-10, tumor necrosis factor α(TNF-α) in serum. Western blot was used to detect the expression of vascular endothelial growth factor A(VEGFA) protein in the cerebral ischemic penumbra. RESULTS: Using network pharmacology and molecular docking validation, four active ingredients(lignan, naringenin, aloe-rhodopsin, and β-sitosterol), seven target proteins(protein kinase b 1, IL-6, TNF, VEGFA, TP53, jun proto-oncogene, and cysteinyl aspartate specific proteinase 3), and inflammatory signaling pathways were identified. Animal experiments showed that the SH and AE groups had fewer neurological deficits, reduced brain infarct volumes, decreased serum inflammatory factor levels, increased expression of VEGFA protein, an

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