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A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level

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Neuroscience Bulletin 2013年第3期29卷 348-354页

作者： Guang-Fei Deng Shu-Jing Liu Xun-Sha Sun wei-Wen Sun Qi-Hua Zhao wei-ping liao Yong-Hong Yi Yue-Sheng Long Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes Guangzhou Medical University

LIM kinase 1 （LIMK1）, a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI （8.0） software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt ＋884 to ＋966 in the human LIMK1 3＇ untranslated region （UTR） was highly conserved in the mouse Limkl 3＇ UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3＇ UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3＇-UTR mutant and neuroloqical disorders.

关键词： LIM kinase 1 region post-transcriptsecondary structurethree-prime untranslatedonal regulation RNA

Carbon steel anticorrosion performance and mechanism of sodium lignosulfonate

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Rare Metals 2024年第1期43卷 356-365页

作者： Bo-Kai liao Rui-Xuan Quan ping-Xian Feng Huan Wang wei Wang Li Niu School of Chemistry and Chemical Engineering Guangzhou Key Laboratory of Sensing Materials and DevicesJoint Institute of Guangzhou University and Institute of Corrosion Science and TechnologyGuangzhou UniversityGuangzhou 510006China

Lignin is a typical biological macromolecule with a three-dimensional network structure and abundant functional groups. It has excellent ionic complexation ability and amphiphilic molecular structure *** this study, the carbon steel anticorrosion performance of sodium lignosulfonate(SLS) in an acid solution was evaluated using the weight loss method, electrochemical measurements, scanning vibration electrode technique(SVET), and surface characterization methods. SLS exhibited excellent corrosion inhibition efficiency for Q235carbon steel in 1 mol·L^(-1) HCl, reaching a maximum value of 98%. A low SLS concentration of 20 mg·L^(-1) resulted in the maximum corrosion inhibition efficiency, which remained nearly constant at higher SLS *** SVET test demonstrated that the formation of an SLS adsorption film can impede corrosion. This study confirms the significance of the application of green biomass resources in the field of metal corrosion protection and green functional materials.

关键词： Lignin Sodium lignosulfonate Corrosion inhibitor Carbon Steel

Functional Investigation of a GRIN2A Variant Associated with Rolandic Epilepsy

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Neuroscience Bulletin 2018年第2期34卷 237-246页

作者： Xing-Xing Xu Xiao-Rong Liu Cui-Ying Fan Jin-Xing Lai Yi-Wu Shi wei Yang Tao Su Jun-Yu Xu Jian-Hong Luo wei-ping liao Department of Neurobiology Key Laboratory of Medical Neurobiology (Ministry of Health of China) Collaborative Innovation Center for Brain Science Zhejiang University School of Medicine Institute of Neuroscience Department of the Second Affiliated Hospital of Guangzhou Medical University Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China

N-methyl-D-aspartate receptors（NMDARs）, a subtype of glutamate-gated ion channels, play a central role in epileptogenesis. Recent studies have identified an increasing number of GRIN2 A（a gene encoding the NMDAR GluN2A subunit） mutations in patients with epilepsy. Phenotypes of GRIN2 A mutations include epilepsy-aphasia disorders and other epileptic encephalopathies, which pose challenges in clinical treatment. Here we identified a heterozygous GRIN2 A mutation（c.1341 T[A, p.N447 K） from a boy with Rolandic epilepsy by whole-exome sequencing. The patient became seizurefree with a combination of valproate and *** investigation was carried out using recombinant NMDARs containing a GluN2A-N447 K mutant that is located in the ligand-binding domain of the GluN2A subunit. Whole-cell current recordings in HEK 293 T cells revealed that the N447 K mutation increased the NMDAR current density by;.2-fold, enhanced the glutamate potency by 2-fold, and reduced the sensitivity to Mg;inhibition. These results indicated that N447 K is a gain-offunction mutation. Interestingly, alternative substitutions by alanine and glutamic acid at the same residue（N447 A and N447 E） did not change NMDAR function, suggesting a residual dependence of this mutation in altering NMDAR function. Taken together, this study identified human GluN2A N447 K as a novel mutation associated with epilepsy and validated its functional consequences in *** of this mutation is also helpful for advancing our understanding of the role of NMDARs in epilepsy and provides new insights for precision therapeutics in epilepsy.

关键词： Epilepsy NMDA receptors GRIN2A Mutation

Epilepsy Genetics:Associations with EEG changes and the underlying mechanism

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Epilepsy Genetics:Associations with EEG changes and the unde...

第三届CAAE中国脑电图和神经电生理大会

作者： wei-ping liao

It is well known that generalized epilepsy differs from partial epilepsy in many clinical and electroencephalo-graphical aspects;and there are also cases with features of both generalized and partial *** studies have demonstrated their differences in genetics,which provided an inside of the molecular basis and underlying

关键词：

Generalized Epilepsy Versus Partial Epilepsy

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Generalized Epilepsy Versus Partial Epilepsy

中华医学会神经病学分会第十次全国脑电图与癫痫诊治进展高级讲授班及学术研讨会

作者： wei-ping liao Institute of Neuroscience of Guangzhou Medical University & Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China

It is well known that generalized epilepsy differs from partial epilepsy in many clinical and electroencephalographical aspects;and there are also cases with features of both generalized and partial *** studies have demonstrated their differences in genetics,which provided an inside of the molecular basis and underlying mechanism of *** are certain genes that tend to associate with generalized *** an example,absence seizures

关键词：

Epilepsy Genetics: generalized epilepsy v.s. partial epilepsy

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Epilepsy Genetics: generalized epilepsy v.s. partial epileps...

2012年浙江省神经病学学术年会

作者： wei-ping liao Institute of Neuroscience of Guangzhou Medical University & Key Laboratory of Neurogenetics and Channelopathies of the Ministry of Education of China

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关键词：

The molecular bases of generalized epilepsy,partial epilepsy,and epilepsy with both generalized and partial features

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The molecular bases of generalized epilepsy,partial epilepsy...

中国神经科学学会第十届全国学术会议

作者： wei-ping liao Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China

Generalized epilepsy differs from partial epilepsy in many clinical and electroencephalographical aspects;there are also cases with features of both generalized and partial *** is hoped that genetic findings will provide insights into their distinctions in molecular *** review the gene abnormalities identified in human epilepsy and analyze their correlations with epilepsy *** explore the potential relationship between a functional pathway and a kind of epilepsy,we analyze genes in an order according to meir *** is found that epilepsies caused by channels or channel-regulatory gene mutations are eiuier generalized or partial epilepsies in the majority;whereas most of the genetic disorders with general cellular dysfunction or brain developmental malformations are associated with epilepsies that exhibit commonly bom generalized and partial features,or are undetermined whether generahzed or *** are apparent overlaps of genetic abnormalities among generalized epilepsies,potentially suggesting a relatively common underlying *** contrast,genetic partial epilepsies appear relatively specific bom clinically and *** explain this,involvement of specific systems of gene expression should be *** present data suggests that each of generahzed epilepsy,partial epilepsy,and epilepsy with bom generahzed and partial features has their own molecular ***,while it is considered that epilepsy is due to an increased excitability or decreased inhibition of the brain,attentions should be paid to the molecular bases of different epilepsies,which are important in serving as indicators of prognosis and for clinical management of epilepsy.

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Privacy-Preserving Consensus-Based Distributed Economic Dispatch of Smart Grids via State Decomposition

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1250-1261页

作者： wei Chen Guo-ping Liu The authors are with the Center for Control Science and Technology Southern University of Science and TechnologyShenzhen 518055China

This paper studies the privacy-preserving distributed economic dispatch(DED)problem of smart *** autonomous consensus-based algorithm is developed via local data exchange with neighboring nodes,which covers both the islanded mode and the grid-connected mode of smart *** prevent power-sensitive information from being disclosed,a privacy-preserving mechanism is integrated into the proposed DED algorithm by randomly decomposing the state into two parts,where only partial data is *** objective is to develop a privacy-preserving DED algorithm to achieve optimal power dispatch with the lowest generation cost under physical constraints while preventing sensitive information from being *** this end,a comprehensive analysis framework is established to ensure that the proposed algorithm can converge to the optimal solution of the concerned optimization problem by means of the consensus theory and the eigenvalue perturbation *** particular,the proposed autonomous algorithm can achieve a smooth transition between the islanded mode and the grid-connected ***,rigorous analysis is given to show privacy-preserving performance against internal and external ***,case studies illustrate the feasibility and validity of the developed algorithm.

关键词： preserving autonomous mode

Antibacterial properties of cerium oxide nanoparticles: Recent progresses and future challenges

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PARTICUOLOGY 2024年第10期93卷 264-283页

作者： Zheng, Heishu Tang, Jiajing wei, Yanhua Deng, Xi Zhang, Yingjuan Ma, Xiangyu Jiang, Xudong Xu, Zhi ping liao, Hongbing Guangxi Med Univ Coll & Hosp Stomatol Nanning 530021 Peoples R China Guangxi Med Univ Guangxi Key Lab Oral & Maxillofacial Rehabil & Rec Nanning 530021 Peoples R China Guangxi Med Univ Affiliated Hosp 1 Nanning 530021 Peoples R China Guangxi Univ Sci & Technol Sch Med Liuzhou 545005 Peoples R China Shenzhen Bay Lab Inst Biomed Hlth Technol & Engn Shenzhen 518107 Peoples R China Shenzhen Bay Lab Inst Syst & Phys Biol Shenzhen 518107 Peoples R China Univ Queensland Australian Inst Bioengn & Nanotechnol Bld 75 St Lucia Qld 4074 Australia

Use of cerium oxide nanoparticles (CeO2 2 NPs) to optimize management of resistant microorganisms has received increasing attention due to non-specific activity of inorganic antibacterial agents. Understanding the mechanism of action is essential to elucidating the antibacterial activity of CeO2 2 NPs against bacteria. Therefore, this review aims to summarize the antibacterial mechanisms of CeO2 2 NPs and correlate the structural and physicochemical properties of CeO2 2 NPs to their antibacterial activity. We further summarize the strategies for the improvement of the antibacterial performance of CeO2 2 NPs and provide our opinions for future challenges as a conclusion. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences.

关键词： Nanoparticles Cerium oxide Antibacterial activity Nanozyme Fenton reaction CEO2 NANOPARTICLES GREEN SYNTHESIS BIOFILM FORMATION ESCHERICHIA-COLI OXIDATIVE STRESS LEAF EXTRACT GROWTH TOXICITY MECHANISMS NANOMATERIALS