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The presence of phosphorylation form of D1 protein in its cross-linked aggregates in high light treated spinach leaves in vivo

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Chinese Science Bulletin 2006年第1期51卷 69-74页

作者： wei Huimin guo Junwei zhang Shuang HUANG Bo LIU Yinqiu DU Linfang College of Life Science Sichuan University Chengdu 610064 China

In the present study, using specific antibody against D1 protein, we detected four ag- gregates of D1 protein in thylakoid membranes from spinach leaves illuminated at high light (800―2500 μmol photons·m?2·s?1) for 3 h. Their accumulations were dependent on the light intensity to which the leaves had been subjected. Further immunoblot analysis indicated that 70 kD aggregate was a prod- uct of D1 protein cross-linked with CP43, 65 and 60 kD aggregate were two cross-linked products be- tween D1 and D2 proteins, and 41 kD aggregate was one cross-linked D1 with α-subunit of cytochrome b559 (Cyt b559). This result provided the evidence for the existence of the aggregation of the D1 protein in vivo. The maximal level of D1/Cyt b559 aggregate occurred at 1000 μmol photons·m?2·s?1 but drastically decreased with further increasing light intensity. Im- munoblot analysis with phosphothreonine (Thr (P)) antibody indicated that D1/CP43 and D1/Cyt b559 aggregates contained the phosphorylated protein(s). In vitro dephosphorylation experiment also showed that D1/Cyt b559 and D1/CP43 aggregates lost the immunoreactivity with Thr (P) antibody after the phosphatase treatment of the membranes from high-light-illuminated leaves. Our results demon- strated that strong illumination of spinach leaves in- duced cross-linked products of D1 protein with its nearby polypeptides of PS Ⅱ , some of which con- tained the phosphorylated D1 protein.

关键词： D1蛋白质磷酸化菠菜强光处理叶片

Atypical presentation of shoulder brucellosis misdiagnosed as subacromial bursitis:A case report

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World Journal of Clinical Cases 2021年第4期9卷 927-934页

作者： Fu-Sheng Wang Khurram Shahzad wei-guo zhang Jie Li Kang Tian Department of Bone and Joint First Hospital of Dalian Medical UniversityDalian 116000Liaoning ProvinceChina

BACKGROUND Brucella infections in the shoulder joint are uncommon,and few have been reported in the *** SUMMARY A 26-year-old male was admitted to our hospital with complaint of recurrent pain and limited movement of the right *** patient reported the pain to have first occurred as an isolated event 6 mo previously and to have reoccurred 5 mo later,when it was accompanied by limited movement of the *** from physical examination and magnetic resonance imaging(referred to as MRI)suggested the diagnosis of subacromial bursitis,and diagnostic paracentesis and arthroscopic debridement were ***,synovial fluid culture detected brucella,and the finding was confirmed by mass spectrometry of a colony *** diagnosis was corrected to brucellosis of the shoulder joint,and antibiotic drug treatment(oral rifampicin and doxycycline)was administrated for 6 *** 4-mo postoperative follow-up examination(MRI)yielded normal *** 2-year follow-up showed no signs of *** This rare case of brucellosis infection in shoulder highlights the importance of increasing awareness to help avoid misdiagnosis.

关键词： Shoulder Misdiagnosis Brucellosis Subacromial bursitis Brucella Case report

NUMERICAL SIMULATION OF CAVITATION FLOW UNDER HIGH PRESSURE AND TEMPERATURE

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Journal of Hydrodynamics 2011年第3期23卷 289-294页

作者： ZHAO wei-guo zhang Ling-xin SHAO Xue-ming Department of Mechanics Zhejiang University Hangzhou 310027 China State Key Laboratory of Fluid Power Transmission and Control Zhejiang University Hangzhou 310027 China

The numerical simulation of cavitation flow on a 2D NACA0015 hydrofoil under high pressure and temperature is performed. The Singhal＇s cavitation model is adopted combined with an improved RNG k-ε turbulence model to study the cavitation flow. The thermal effect in the cavitation flow is taken into account by introducing the energy equation with a source term based on the latent heat transfer. The code is validated by a case of a hydrofoil under two different temperatures in a comparison between the simulation and the experiment. Computational results show that the latent heat of vaporization has a significant impact on the cavitation process in the high temperature state, and the cavity in the high temperature state is thinner and shorter than that in a normal state with the same cavitation number, due to the fact that the heat absorption in the cavitation area reduces the local temperature and the saturated vapor pressure. This numerical study provides some guidance for the design of machineries in the High Pressure and Temperature （HPT） state.

关键词： cavitation NACA0015 thermodynamic CFD high pressure and temperature

Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork

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International Journal of Ophthalmology(English edition) 2022年第8期15卷 1240-1248页

作者： Miao wei Lu-Ming Chen Ze-Yu Huang guo-wei zhang Huai-Jin Guan Min Ji Eye Institute Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Dalian Medical University Dalian 116000Liaoning ProvinceChina

AIM:To investigate potential gene changes in trabecular meshwork(TM)induced by dexamethasone(DEX)in steroidinduced glaucoma(SIG).METHODS:The expression data of 24 cases from a public functional genomics data were sorted to identify the mechanisms of action of DEX on the *** relationships of the differentially expressed genes(DEGs)were enriched using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)*** addition,the hub genes were screened by the Search Tool for the Retrieval of Interacting Genes Database(STRING)and Cytoscape ***,human TM cells(HTMCs)were treated with DEX to preliminarily explore the function of hub ***:Totally 47 DEGs,including 21 downregulated and 26 upregulated genes were *** primary enriched results of the DEGs consisted of inflammatory response,extracellular matrix(ECM),negative regulation of cell proliferation,TNF signalling pathway and the regulation of tr yptophan channels by inflammator y ***,pro-melanin-enriched hormone(PMCH)and Bradykinin B1 receptor(BDKRB1)were screened as hub *** is verified in GSE37474 data *** blot and quantitative real-time polymerase chain reaction(q PCR)results showed that protein and RNA expression levels of BDKRB1 were significantly decreased after DEX treatment,while PMCH was not significantly ***:BDKRB1 may be a key gene involved in SIG onset,providing a suitable therapeutic target for improving the prognosis of SIG patients.

关键词： dexamethasone trabecular meshwork cells steroid-induced glaucoma differentially expressed genes protein-protein interaction

Fenestrated endovascular aortic repair for juxtarenal abdominal aortic aneurysm

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Chinese Medical Journal 2013年第3期126卷 409-414页

作者： guo wei zhang Hong-peng LIU Xiao-ping JIA Xin XIONG Jiang MA Xiao-hui Department of Vascular Surgery Chinese People's Liberation Army General Hospital Beijing 100853 China

Background Endovascular stent-graft with fenestration can improve proximal sealing in patients with juxtarenal abdominal aortic aneurysm （JAAA）. The purpose of this study was to describe our primary experience and evaluate the safety and efficacy of fenestrated device for JAAA in high-risk patients. Methods Between March 2011 and May 2012, nine male patients （mean age, （79.6±8.6） years） with asymptomatic JAAAs underwent elective deployment of the Zenith fenestrated stent-grafts at a single institution. All patients were treated in the hybrid operating room under general anesthesia. Follow-up computed tomography angiography （CTA） was routinely performed before discharge, at 3, 6, and 12 months and annually thereafter. Results Procedural success was achieved in all cases. Total sixteen small fenestrations, two large fenestrations and eight scallops were used. Intra-operative complications occurred in four patients, which included one proximal type I endoleak, two type II endoleaks, and one renal artery dissection. The mean hospital stay was （8.9±1.4） days, mean blood loss was （360.5±46.8） ml, and mean iodinated contrast volume was （230.6±58.3） ml. The mean follow-up time was （7.6±4.2） months. The visceral graft patency was 100% until now. One patient had an increase of serum creatinine of more than 30%, but did not require dialysis. No patients died, no stent fractured, and migration were diagnosed during the follow-up. Conclusions The early results of fenestrated device for high-risk patients with complex JAAAs are satisfactory. However, long-term fenestrated graft durability and branch vessel patency remain to be determined.

关键词： abdominal aortic aneurysm juxtarenal aneurysm endovascular aortic repair, fenestrated stent-graft

Tunable luminescence of Bi^(3+)/Eu^(3+)-doped SiO_(2)-Al_(2)O_(3)-MCO_(3)(M=Ca,Sr,Ba) glasses for WLED

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Journal of Rare Earths 2024年第9期42卷 1652-1657,I0001页

作者： Falin zhang Xiaoying Liang Rongfei wei Hai guo Fangfang Hu Department of Physics Zhejiang Normal UniversityJinhua321004China

In this work,a group of Bi^(3+)/Eu^(3+)-doped SiO2-Al_(2)O_(3)-MCO_(3)(M=Ca,Sr,Ba) glasses were fabricated through melt-quenching *** structure and photoluminescence characters were studied via Xray diffraction(XRD),UV-Vis transmission spectra(TRS),excitation spectra,emission spectra and decay *** XRD results show that no nano-crystal phase forms in glass matrix with different doping concentrations of Bi^(3+)and Eu^(3+)*** regulating optical alkalinity value,the intensity of Bi^(3+)ions increases and the peak position shifts to red *** Bi^(3+)/Eu^(3+) co-doped glasses show a blue-white-yellow-red luminescence by changing concentration of Eu^(3+) *** transfer efficiency from Bi^(3+) to Eu^(3+) can reach as high as 10.54%.At 423 K, the emission intensity of 55SiO_(2)-10Al_(2)O_(3)-35BaO-0.25Bi_(2)O_(3)(GBE0.5) is 31.1% of that at 308 ***,the activation energy of the glass matrix is 0.251 *** results suggest that these Bi^(3+)/Eu^(3+) co-doped glass samples possess potential application in LED field.

关键词： Bi^(3+) Eu^(3+) Glass White light-emitting diode Energy transfer Rare earths

Transumbilical single-port laparoscopic cholecystectomy： a case report

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Chinese Medical Journal 2008年第23期121卷 2463-2464页

作者： guo wei zhang Zhong-tao HAN wei LI Jian-she JIN Lan LIU Jun ZHAO Xiao-mu WANG Yu Department of General Surgery Beijing Friendship Hospital Capital Medical University Beijing 100050 China

Recently, scarless surgery has become a widely used surgical technique, which has made considerable progress owing to extensive animal model studies and clinical trials. Here, we report the first case of transumbilica... 详细信息

关键词： laparoscopic cholecystectomy clinical trials mainland China animal model

Adenovirus expressing p27^(kip1) suppresses growth of established esophageal carcinoma xenografts

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World Journal of Gastroenterology 2005年第42期11卷 6582-6586页

作者： wei-guo zhang Qing-Ming Wu Jie-Ping Yu Qiang Tong guo-Jian Xie Xiao-Hu Wang Sheng-Bao Li Digestive Department TaiheHospital Yunyang Medical College Shiyan 442000 HubeiProvince China Digestive Department Renmin Hospital of WuhanUniversity Wuhan 430060 Hubei Province China

AIM： TO investigate the growth suppression of adenovirus expressing p27^kip1 on established esophageal tumors in nude mice. METHODS： Esophageal carcinoma xenografts in nude mice were established by tumor tissue mass transplantation. The successfully constructed recombinant adenoviral vectors carrying p2^kip1 gene （Ad- p27^%kip1） were directly injected into the esophageal tumors in nude mice. Compared to control group, the growth curve of tumor was drawn and the growth inhibition rate of tumor was calculated. The histology of tumors was examined by hematoxylin and eosin （H＆E） staining. The expression of p27^kip1 and survivin was detected in tumors by immunohistochemical technique. RESULTS： The growth of tumors in gene therapy group with Ad-p27^kip1 was obviously suppressed compared to control group （0.42±0.08 g vs 1.17±0.30 g, t=6.39, P〈0.01）, the inhibition rate of tumor growth reached 64.1%. Pathological detection showed that the tumors in nude mice were poorly differentiated esophageal squarnous carcinoma. In addition, the expression of p27^kip1 was increased, while the expression of survivin was decreased in tumors after being transfected with Ad-p27^kip1. CONCLUSION： p27^kip1 gene therapy mediated by adenovirus vector has a significant inhibitory effect on esophageal carcinoma in vivo. Up-regulated p27^kip1 expression and down-regulated survivin expression may be its important mechanisms.

关键词： p27^kip1 gene Esophageal carcinoma

Flexural behavior of textile reinforced mortar-autoclaved lightweight aerated concrete composite panels

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Frontiers of Structural and Civil Engineering 2024年第5期18卷 776-787页

作者： Liying guo Mingke DENG wei zhang Tong LI Yangxi zhang Mengyu CAO Xian HU Department of Civil Engineering Xi’an University of Architecture and TechnologyXi’an 710055China Key Lab of Structural Engineering and Earthquake Resistance Ministry of Education(XAUAT)Xi’an 710055China Scene engineering design institute Xi’an 710016China Dahua Group Shang hai 200062China

To improve the deficiencies of prefabricated autoclaved lightweight aerated concrete(ALC)panel such as susceptibility to cracking and low load-bearing capacity,a textile-reinforced mortar-autoclaved lightweight aerated concrete(TRM-ALC)composite panel was developed in this *** group of reference ALC panels and five groups of TRM-ALC panels were fabricated and subjected to four-point flexural *** was applied on the tensile side of the ALC panels to create *** variable parameters were the plies of textile(one or two),type of textile(basalt or carbon),and whether the matrix(without textile)was applied on the compression side of *** results showed that a bonding only 8-mm-thick TRM layer on the surface of the ALC panel could increase the cracking load by 180%−520%.The flexural capacity of the TRM-ALC panel increased as the number of textile layers *** reinforcement of the matrix on the compressive side could further enhance the stiffness and ultimate loadbearing capacity of the TRM-ALC *** panels with basalt textile failed in flexural mode,with the rupture of fabric *** with carbon textile failed in shear mode due to the ultra-high tensile strength of *** addition,analytical models related to the different failure modes were presented to estimate the ultimate load-carrying capacity of the TRM-ALC panels.

关键词： prefabricated autoclaved lightweight aerated concrete panel textile-reinforced mortar cracking load flexural capacity stiffness