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Chain Elongation Using Native Soil Inocula:Exceptional n-Caproate Biosynthesis Performance and Microbial Mechanisms

Engineering 2024年第8期39卷 262-272页

作者： Lin Deng Yang Lv Tian Lan Qing-Lian Wu wei-tong ren Hua-Zhe Wang Bing-Jie Ni Wan-Qian Guo State Key Laboratory of Urban Water Resource and the Environment Harbin Institute of TechnologyHarbin 150090China Centre for Technology in Water and Wastewater&School of Civil and Environmental Engineering University of Technology SydneySydneyNSW 2007Australia

This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation(CE)platform,offering new insights into anaerobic soil *** results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate,highlighting the suitability of soil as an ideal source for n-caproate *** with anaerobic sludge and pit mud,the native soil CE system exhibited higher selectivity(60.53%),specificity(82.32%),carbon distribution(60.00%),electron transfer efficiency(165.00%),and conductivity(0.59 ms∙cm^(-1)).Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield.A microbial community analysis indicated a positive correlation between the relative abundances of Pseudomonas,Azotobacter,and Clostridium and n-caproate ***,metagenomics analysis revealed a higher abundance of functional genes in key microbial species,providing direct insights into the pathways involved in n-caproate formation,including in situ CO_(2)utilization,ethanol oxidation,fatty acid biosynthesis(FAB),and reverse beta-oxidation(RBO).The numerous functions in FAB and RBO are primarily associated with Pseudomonas,Clostridium,Rhodococcus,Stenotrophomonas,and Geobacter,suggesting that these genera may play roles that are involved or associated with the CE ***,this innovative inoculation strategy offers an efficient microbial source for n-caproate production,underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration.

关键词： Soil Chain elongation n-caproate Reverse beta-oxidation Fatty acid biosynthesis Metagenomics

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揭示氨对正己酸生产影响的机制——影响途径、关键酶和微生物

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Engineering 2024年第4期35卷 180-190页

作者： Qing-Lian Wu Ke-Xin Yuan wei-tong ren Lin Deng Hua-Zhe Wang Xiao-Chi Feng He-Shan Zheng Nan-Qi ren Wan-Qian Guo State Key Laboratory of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin 150090China College of Architecture and Environment Sichuan UniversityChengdu 610065China State Key Laboratory of Urban Water Resource and Environment School of Civil and Environmental EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China College of Chemistry and Chemical Engineering Qiqihar UniversityQiqihar 161006China

n-Caproate,which is produced via chain elongation(CE)using waste biomass,can supply various fossilderived products,thus advancing the realization of carbon *** released from the degradation of nitrogen-rich waste biomass can act as a nutrient or an inhibitor in anaerobic bioprocesses,including CE,with the distinction being primarily dependent on its ***,the optimal concentration of ammonia and the threshold of toxicity for open-culture n-caproate production using ethanol as an electron donor,along with the underlying mechanisms,remain *** study revealed that the optimal concentration of ammonia for n-caproate production was 2.0 g∙L^(-1),whereas concentrations exceeding this threshold markedly suppressed the CE *** of the mechanism revealed the involvement of two forms of ammonia(i.e.,ammonium ions and free ammonia)in this inhibitory *** ammonia levels(5.0 g∙L^(-1))induced excessive ethanol oxidation and suppressed the reverse β-oxidation(RBO)process,directly leading to the enhanced activities of enzymes(phosphotransacetylase and acetate kinase)responsible for acetate formation and diminished activities of butyryl-coenzyme A(CoA):acetyl-CoA transferase,caproyl-CoA:butyryl-CoA transferase,and caproyl-CoA:acetyl-CoA transferase that are involved in the syntheses of n-butyrate and ***,the composition of the microbial community shifted from Paraclostridium dominance(at 0.1 g∙L^(-1)ammonia)to a co-dominance of Fermentimonas,Clostridium sensu stricto 12,and Clostridium sensu stricto 15 at 2.0 g∙L^(-1)***,these CE-functional bacteria were mostly absent in the presence of excessive ammonia(5.0 g∙L^(-1)ammonia).Metagenomic analysis revealed the upregulation of functions such as RBO,fatty acid synthesis,K^(+)efflux,adenosine triphosphatase(ATPase)metabolism,and metal cation export in the presence of 2.0 g∙L^(-1)ammonia,collectively contributing to enhanced n-caproate produc

关键词： Ammonia inhibition Medium chain fatty acids n-Caproate Enzyme Metagenomics

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Inter-well internal resonance analysis of rectangular asymmetric cross-ply bistable composite laminated cantilever shell under transverse foundation excitation

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Applied Mathematics and Mechanics(English Edition) 2024年第8期45卷 1353-1370页

作者： Lele ren wei ZHANG Yufei ZHANG State Key Laboratory of Mechanical System and Vibration Shanghai Jiao Tong UniversityShanghai 200240China Department of Mechanics Guangxi UniversityNanning 530004China Beijing Key Laboratory of Nonlinear Vibrations and Strength of Mechanical Structures College of Mechanical EngineeringBeijing University of TechnologyBeijing 100124China

The chaotic dynamic snap-through and complex nonlinear vibrations are investigated in a rectangular asymmetric cross-ply bistable composite laminated cantilever shell,in cases of 1:2 inter-well internal resonance and primary *** transverse foundation excitation is applied to the fixed end of the structure,and the other end is in a free *** first-order approximate multiple scales method is employed to perform the perturbation analysis on the dimensionless two-degree-of-freedom ordinary differential motion control *** four-dimensional averaged equations are derived in both polar and rectangular coordinate *** from the obtained frequency-amplitude and force-amplitude response curves,a detailed analysis is conducted to examine the impacts of excitation amplitude,damping coefficient,and tuning parameter on the nonlinear internal resonance characteristics of the *** nonlinear softening characteristic is exhibited in the upper stable-state,while the lower stable-state demonstrates the softening and linearity *** simulation is carried out using the fourth-order Runge-Kutta method,and a series of nonlinear response curves are *** the excitation amplitude further elucidates the global bifurcation and chaotic dynamic snap-through characteristics of the bistable cantilever shell.

关键词： bistable composite laminated cantilever shell inter-well internal resonance primary resonance chaotic dynamic snap-through complex nonlinear vibration

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Apically guiding electron/mass transfer reaction induced by Ag/FeN_(x)Mott-Schottky effect within a hollow star reactor toward high performance zinc-air batteries

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Journal of Energy Chemistry 2024年第8期95卷 106-116,I0004页

作者： Kaixiang Shi Kaixin Wang tong Li Junhao Li Jie ren Xu Li Yonggang Min Zhouguang Lu wei Tan Quanbing Liu Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center(Rongjiang Laboratory) Guangdong University of TechnologyJieyang 515200GuangdongChina Guangzhou Key Laboratory of Clean Transportation Energy Chemistry Guangdong Provincial Key Laboratory of Plant Resources BiorefinerySchool of Chemical Engineering and Light IndustryGuangdong University of TechnologyGuangzhou 510006GuangdongChina Key Laboratory of Electrochemical Energy Storage and Energy Conversion of Hainan Province School of Chemistry and Chemical EngineeringHainan Normal UniversityHaikou 571158HainanChina School of Materials and Energy Guangdong University of TechnologyGuangzhou 510006GuangdongChina Department of Materials Science and Engineering Southern University of Science and TechnologyShenzhen 518055GuangdongChina

The disparity in the transfer of carriers(electrons/mass)during the reaction in zinc-air batteries(ZABs)results in sluggish kinetics of the oxygen reduction reaction(ORR)and oxygen evolution reaction(OER),along with elevated overpotentials,thereby imposing additional constraints on its ***,the pre-design and target-development of inexpensive,high-performance,and long-term stable bifunctional catalysts are urgently *** this work,an apically guiding dual-functional electrocatalyst(Ag-FeN_(x)-N-C)was prepared,in which a hierarchical porous nitrogen-doped carbon with three-dimensional(3D)hollow star-shaped structure is used as a substrate and high-conductivity Ag nanoparticles are coupled with iron nitride(FeN_(x))*** calculations indicate that the Mott-Schottky heterojunction as an inherent electric field comes from the two-phase bound of Ag and FeN_(x),of which electron accumulation in the FeN_(x)phase region and electron depletion in the Ag phase region promote orientated-guiding charge *** effective modulation of local electronic structures felicitously reforms the d-band electron-group distribution,and intellectually tunes the masstransfer reaction energy barriers for both ORR/***,the hollow star-s haped hierarchical porous structure provides an apical region for fast mass *** results show that the halfwave potential for ORR is 0.914 V,and the overpotential for OER is only 327 mV at 10 mA cm^(-2).A rechargeable ZAB with Ag-FeN_(x)-N-C as the air cathode demonstrates long-term cycling performance exceeding 1500 cycles(500 h),with a power density of 180 mW cm^(-2).Moreover,when employing AgFeN_(x)-N-C as the air cathode,flexible ZABs demonstrate a notable open-circuit voltage of 1.42 V and achieve a maximum power density of 65.6 mW cm^(-2).Ag-FeN_(x)-N-C shows guiding electron/mass transfer route and apical reaction microenvironment for the electrocatalyst architecture in the explo

关键词： Hollow star structure Mott-Schottky effect Apically guiding effect Bifunctional catalysis Zinc-air batteries

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A Hybrid Feature Fusion Traffic Sign Detection Algorithm Based on YOLOv7

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Computers, Materials & Continua 2024年第7期80卷 1425-1440页

作者： Bingyi ren Juwei Zhang tong Wang School of Mechanical Engineering Henan University of Science and TechnologyLuoyang471000China School of Information Engineering Henan University of Science and TechnologyLuoyang471000China School of Electronic Information Zhengzhou Institute of Aeronautical Industry ManagementZhengzhou450046China Henan Province New Energy Vehicle Power Electronics and Power Transmission Engineering Research Center Luoyang471000China

Autonomous driving technology has entered a period of rapid development,and traffic sign detection is one of the important *** target detection networks are difficult to adapt to scenarios where target sizes are seriously imbalanced,and traffic sign targets are small and have unclear features,which makes detection more ***,we propose aHybrid Feature Fusion Traffic Sign detection algorithmbased onYOLOv7(HFFTYOLO).First,a self-attention mechanism is incorporated at the end of the backbone network to calculate feature interactions within scales;Secondly,the cross-scale fusion part of the neck introduces a bottom-up multi-path fusion *** reuse paths at the end of the neck,paying particular attention to cross-scale fusion of highlevel *** addition,we found the appropriate channel width through a lot of experiments and reduced the superfluous *** terms of training,a newregression lossCMPDIoUis proposed,which not only considers the problem of loss degradation when the aspect ratio is the same but the width and height are different,but also enables the penalty term to dynamically change at different ***,our proposed improved method shows excellent results on the TT100K *** with the baseline model,without increasing the number of parameters and computational complexity,AP0.5 and AP increased by 2.2%and 2.7%,respectively,reaching 92.9%and 58.1%.

关键词： Small target detection YOLOv7 traffic sign detection regression loss

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An automatic Q-factor matching method for eliminating 77%of the ZRO of a MEMS vibratory gyroscope in rate mode

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Microsystems & Nanoengineering 2024年第3期10卷 35-48页

作者： Jingbo ren tong Zhou Yi Zhou Yixuan Li Yan Su School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing 210000China

Mismatching quality factors(Q-factors)is one of the main factors causing zero-rate output(ZRO)in degenerate(DE)Micro-Electro-Mechanical Systems(MEMS)vibratory *** eliminate the ZRO of the DE MEMS gyroscope,this study introduces a method for real-time identification and automatic matching of Q-factors in rate *** leveraging the vibration characteristics of the DE MEMS vibratory gyroscope in rate mode,dedicated online test methods are designed to determine the Q-factors for both the drive and sense modes,enabling online identification of the Q-factor ***,an automatic Q-factor matching system is designed utilizing the mechanical-thermal dissipation mechanism of the resistive *** effectiveness of this proposed method is validated through simulations and experiments conducted on a MEMS disk resonator gyroscope(DRG).The results show a measurement error within 4%for Q-factor identification,and automatic Q-factor matching effectively reduces the ZRO by 77%.Employing this automatic Q-factor matching method successfully reduces the ZRO that is caused by the mismatching of Q-factors in the MEMS DRG from 0.11°/s to 0.025°/s and improves the bias instability(BI)from 0.40°/s to 0.19°/s.

关键词： matching mode method

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Image attention transformer network for indoor 3D object detection

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Science China(Technological Sciences) 2024年第7期67卷 2176-2190页

作者： ren KeYan YAN tong HU ZhaoXin HAN HongGui ZHANG YunLu Faculty of Information Technology Beijing University of TechnologyBeijing 100124China

Point clouds and RGB images are both critical data for 3D object detection. While recent multi-modal methods combine them directly and show remarkable performances, they ignore the distinct forms of these two types of data. For mitigating the influence of this intrinsic difference on performance, we propose a novel but effective fusion model named LI-Attention model, which takes both RGB features and point cloud features into consideration and assigns a weight to each RGB feature by attention ***, based on the LI-Attention model, we propose a 3D object detection method called image attention transformer network(IAT-Net) specialized for indoor RGB-D scene. Compared with previous work on multi-modal detection, IAT-Net fuses elaborate RGB features from 2D detection results with point cloud features in attention mechanism, meanwhile generates and refines 3D detection results with transformer model. Extensive experiments demonstrate that our approach outperforms stateof-the-art performance on two widely used benchmarks of indoor 3D object detection, SUN RGB-D and NYU Depth V2, while ablation studies have been provided to analyze the effect of each module. And the source code for the proposed IAT-Net is publicly available at https://***/wisper181/IAT-Net.

关键词： 3D object detection transformer attention mechanism

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Finite-size-induced non-Hermitian phase transitions in real space

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Science China(Physics,Mechanics & Astronomy) 2024年第11期Mechanics & Astronomy) .卷 109-115页

作者： Hongfei Wang Biye Xie wei ren Department of Physics International Center for Quantum and Molecular Structures Shanghai University School of Science and Engineering The Chinese University of Hong Kong

While non-Hermiticity provokes intriguing phenomena without Hermitian counterparts, e.g., the skin effect and the breakdown of bulk-boundary correspondence, attracting extensive attention both in fundamental physics and device engineering, the role of finite sizes therein remains elusive. Here, we propose a class of finite-size-induced non-Hermitian phase transitions, relying upon higher-order topological invariants associated with real-space wave functions. The phase diagrams for general non-Hermitian chiral models are further acquired to demonstrate our topological definition. Such phase transitions are elucidated qualitatively by an effective intercell coupling alteration that depends on finite sizes in respective directions. Besides, we mimic these phenomena by analogizing the circuit Laplacian in finite-size electric circuits with nonreciprocal couplings. The resultant admittance spectra agree with our theoretical predictions. Our findings shed light on the finite-size mechanism of non-Hermitian topological phase transitions and pave the way for applications in switching and sensing.

关键词： non-Hermitian phase transitions finite-size effects higher-order topological invariants

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Snap-through behaviors and nonlinear vibrations of a bistable composite laminated cantilever shell:an experimental and numerical study

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Applied Mathematics and Mechanics(English Edition) 2024年第5期45卷 779-794页

作者： Lele ren wei ZHANG Ting DONG Yufei ZHANG State Key Laboratory of Mechanical System and Vibration Shanghai Jiao Tong UniversityShanghai 200240China Department of Mechanics Guangxi UniversityNanning 530004China Beijing Key Laboratory of Nonlinear Vibrations and Strength of Mechanical Structures College of Mechanical EngineeringBeijing University of TechnologyBeijing 100124China School of Mechanical Engineering Beijing Institute of TechnologyBeijing 100081China

The snap-through behaviors and nonlinear vibrations are investigated for a bistable composite laminated cantilever shell subjected to transversal foundation excitation based on experimental and theoretical *** improved experimental specimen is designed in order to satisfy the cantilever support boundary condition,which is composed of an asymmetric region and a symmetric *** symmetric region of the experimental specimen is entirely clamped,which is rigidly connected to an electromagnetic shaker,while the asymmetric region remains free of *** motion paths are realized for the bistable cantilever shell by changing the input signal levels of the electromagnetic shaker,and the displacement responses of the shell are collected by the laser displacement *** numerical simulation is conducted based on the established theoretical model of the bistable composite laminated cantilever shell,and an off-axis three-dimensional dynamic snap-through domain is *** numerical solutions are in good agreement with the experimental *** nonlinear stiffness characteristics,dynamic snap-through domain,and chaos and bifurcation behaviors of the shell are quantitatively *** to the asymmetry of the boundary condition and the shell,the upper stable-state of the shell exhibits an obvious soft spring stiffness characteristic,and the lower stable-state shows a linear stiffness characteristic of the shell.

关键词： bistable composite laminated cantilever shell snap-through behavior nonlinear vibration nonlinear stiffness characteristic chaos and bifurcation

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A Feature Selection Method Based on Hybrid Dung Beetle Optimization Algorithm and Slap Swarm Algorithm

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Computers, Materials & Continua 2024年第8期80卷 2979-3000页

作者： wei Liu Tengteng ren School of Information Science and Engineering Shenyang Ligong UniversityShenyang110158China

Feature Selection(FS)is a key pre-processing step in pattern recognition and data mining tasks,which can effectively avoid the impact of irrelevant and redundant features on the performance of classification *** recent years,meta-heuristic algorithms have been widely used in FS problems,so a Hybrid Binary Chaotic Salp Swarm Dung Beetle Optimization(HBCSSDBO)algorithm is proposed in this paper to improve the effect of *** this hybrid algorithm,the original continuous optimization algorithm is converted into binary form by the S-type transfer function and applied to the FS *** combining the K nearest neighbor(KNN)classifier,the comparative experiments for FS are carried out between the proposed method and four advanced meta-heuristic algorithms on 16 UCI(University of California,Irvine)*** evaluation metrics such as average adaptation,average prediction accuracy,and average running time are chosen to judge and compare the *** selected dataset is also discussed by categorizing it into three dimensions:high,medium,and low *** results show that the HBCSSDBO feature selection method has the ability to obtain a good subset of features while maintaining high classification accuracy,shows better optimization *** addition,the results of statistical tests confirm the significant validity of the method.

关键词： Feature selection dung beetle optimization KNN transfer function HBCSSDBO

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