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Stress Responsive Zinc-finger Protein Gene of Populus euphratica in Tobacco Enhances Salt Tolerance

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Journal of Integrative Plant Biology 2008年第1期50卷 56-61页

作者： Jun-ying Wang Xin-Li Xia Jun-Ping Wang wei-lun yin Biotechnology Research Institute of Chinese Academy of Agncultural Sciences Beijing 100081 China Beijing Forestry University Beijing 100083 China Tianjin University of Science and Technology Tianjin 300222 China

The Populus euphratica stress responsive zinc-finger protein gene PSTZ, which encodes a protein including typical Cys2/His2 zinc finger structure, was isolated by reverse transcription-polymerase chain reaction from P. euphratica. Northern hybridization revealed that its expression was induced under drought and salt stress conditions. To examine its function, cDNA of the PSTZ gene, driven by the cauliflower mosaic virus 35S promoter, was cloned into a plant expression vector pBin438 and introduced into tobacco plants. Transgenic tobacco showed an enhanced salt tolerance, suggesting that PSTZ may play a role in plant responsiveness to salt stress.

关键词： enhance Populus euphratica, salt tolerance stress responsive zinc-finger protein.

Growth, Gas Exchange, Abscisic Acid, and Calmodulin Response to Salt Stress in Three Poplars

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Journal of Integrative Plant Biology 2006年第3期48卷 286-293页

作者： Yu Chang Shao-Liang Chen wei-lun yin Rui-Gang Wang Yan-Feng Liu Yong Shi Yuan-Yuan Shen Yue Li Jie Jiang Yue Liu College of Biological Science and Technology Beijing Forestry University Beijing 100083 China

In the present study, we Investigated the effects of Increaslng sallnlty on growth, gas exchange, absclslc acld （ABA）, calmodulln （CAM）, and the relevance to salt tolerance In seedllngs of Populus euphratlca Ollv. and cutUngs of P. ＂pupularls 35-44＂（P. popularls） and P. x euramerlcana cv. 1-214 （P. cv. Itallca）. The relatlve growth rates of shoot helght （RGR,） for P. cv. Itallca and P. popularls were severely reduced by Increaslng salt stress, whereas the growth reductlon was relatlvely less in P. euphratica. Slmllarly, P. euphratlca malntalned hlgher net photosynthetlc rates （Pn） and unlt transplration rotes （TRN） than P. cv. Itallca and P. popularls under condltlons of hlgher sallnlty. Sallnity caused a slgnlficant increase In leaf ABA and CaM In the three genotypes after the onset of stress, but NaCl-induced ABA and CaM accumulatlon was more pronounced In P. euphratlca, suggeeUng that P. euphratlca plants are more sensitlve in sensing soil salinlty than the other two poplars. Furthermore, P. euphratica maintained relatively higher ABA and CaM concentrations under conditions of high salinity. The higher capacity to synthesize stress signals, namely ABA and CaM, In P. euphratica and the contrlbuUon of this to the salt resistance of P. euphratica are discussed.

关键词： abscisic acid calmodulin gas exchange growth NaCl Populus euphratica.

Physiological characterization,transcriptomic profiling,and microsatellite marker mining of Lycium ruthenicum

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第11期18卷 1002-1021页

作者： Jin-huan CHEN Dong-zhi ZHANG Chong ZHANG Mei-long XU wei-lun yin College of Biological Sciences and Technology Beijing Forestry UniversityBeijing 100083China National Engineering Laboratory for Tree Breeding Beijing Forestry UniversityBeijing 100083China State Key Laboratory of Seedling Bioengineering Yinchuan 750004China

Lycium ruthenicum is a perennial shrub species that has attracted considerable interest in recent years owing to its nutritional value and ability to thrive in a harsh environment. However, only extremely limited transcriptomic and genomic data related to this species can be found in public databases, thereby limiting breeding research and molecular function analysis. In this study, we characterized the physiological and biochemical responses to saline- alkaline mixed stress by measuring photochemical efficiency, chlorophyll content, and protective enzyme activity. We performed global transcriptomic profiling analysis using the IIlumina platform. After optimizing the assembly, a total of 68063 unique transcript sequences with an average length of 877 bp were obtained. Among these sequences, 4096 unigenes were upregulated and 4381 unigenes were down-regulated after saline-alkaline mixed treatment. The most abundant transcripts and over-represented items were assigned to gene ontology （GO） terms or Kyoto Encyclopedia of Genes and the Genomes （KEGG） categories for overall unigenes, and differentially expressed unigenes were analyzed in detail. Based on this set of RNA-sequencing data, a total of 9216 perfect potential simple sequence re- peats （SSRs） were identified within 7940 unigenes with a frequency of 1/6.48 kb. A total of 77 primer pairs were synthesized and examined in wet-laboratory experiments, of which 68 loci （88.3%） were successfully amplified with specific products. Eleven pairs of polymorphic primers were verified in 225 individuals from nine populations. The inbreeding coefficient and the polymorphism information content value ranged from 0.011 to 0.179 and from 0.1112 to 0.6750, respectively. The observed and expected heterozygosities ranged from 0.064 to 0.840 and from 0.115 to 0.726, respectively. Nine populations were clustered into three groups based on a genetic diversity study using these novel markers. Our data will be useful for functional genom

关键词： De novo Genetic diversity Lycium ruthenicum Molecular marker Saline-alkaline mixed stress

Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchid evolution

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Horticulture Research 2021年第1期8卷 2684-2697页

作者： Yongxia Zhang Guo-Qiang Zhang Diyang Zhang Xue-Die Liu Xin-Yu Xu wei-Hong Sun Xia Yu Xiaoen Zhu Zhi-Wen Wang Xiang Zhao Wen-ying Zhong Hongfeng Chen wei-lun yin Tengbo Huang Shan-Ce Niu Zhong-Jian Liu Guangdong Provincial Key Laboratory for Plant Epigenetics College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518071China Laboratory for Orchid Conservation and Utilization Orchid Conservation and Research CenterThe National Orchid Conservation CenterShenzhen 518114China School of Food Science and Technology Foshan UniversityFoshan 528225China Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou 350002China College of Forestry Fujian Agriculture and Forestry UniversityFuzhou 350002China PubBio-Tech Wuhan 430070China Key Laboratory of Plant Resources Conservation Sustainable Utilization South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China College of Biological Sciences and Technology Beijing Forestry UniversityBeijing 100083China College of Horticulture Hebei Agricultural UniversityBaoding 071000China

As one of the largest families of angiosperms,the Orchidaceae family is *** represents the second largest genus of the ***,an assembled high-quality genome of species in this genus is ***,we report a chromosome-scale reference genome of Dendrobium chrysotoxum,an important ornamental and medicinal orchid *** assembled genome size of *** was 1.37 Gb,with a contig N50 value of 1.54 *** the sequences,95.75%were anchored to 19 *** were 30,044 genes predicted in the *** *** whole-genome polyploidization events occurred in *** terms of the second event,whole-genome duplication(WGD)was also found to have occurred in other Orchidaceae members,which diverged mainly via gene loss immediately after the WGD event occurred;the first duplication was found to have occurred in most monocots(tau event).We identified sugar transporter(SWEET)gene family expansion,which might be related to the abundant medicinal compounds and fleshy stems of ***-box genes were identified in ***,as well as members of TPS and Hsp90 gene families,which are associated with resistance,which may contribute to the adaptive evolution of *** also investigated the interplay among carotenoid,ABA,and ethylene biosynthesis in *** to elucidate the regulatory mechanisms of the short flowering period of orchids with yellow *** reference *** genome will provide important insights for further research on medicinal active ingredients and breeding and enhances the understanding of orchid evolution.

关键词： evolution flowers breeding

Research on Stock Price Prediction Method Based on the GAN-LSTM-Attention Model

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Computers, Materials & Continua 2025年第1期82卷 609-625页

作者： Peng Li Yanrui wei Lili yin College of Computer Science and Technology Harbin University of Science and TechnologyHarbin150006China

Stock price prediction is a typical complex time series prediction problem characterized by dynamics,nonlinearity,and *** paper introduces a generative adversarial network model that incorporates an attention mechanism(GAN-LSTM-Attention)to improve the accuracy of stock price ***,the generator of this model combines the Long and Short-Term Memory Network(LSTM),the Attention Mechanism and,the Fully-Connected Layer,focusing on generating the predicted stock *** discriminator combines the Convolutional Neural Network(CNN)and the Fully-Connected Layer to discriminate between real stock prices and generated stock ***,to evaluate the practical application ability and generalization ability of the GAN-LSTM-Attention model,four representative stocks in the United States of America(USA)stock market,namely,Standard&Poor’s 500 Index stock,Apple Incorporatedstock,AdvancedMicroDevices Incorporatedstock,and Google Incorporated stock were selected for prediction experiments,and the prediction performance was comprehensively evaluated by using the three evaluation metrics,namely,mean absolute error(MAE),root mean square error(RMSE),and coefficient of determination(R2).Finally,the specific effects of the attention mechanism,convolutional layer,and fully-connected layer on the prediction performance of the model are systematically analyzed through ablation *** results of experiment show that the GAN-LSTM-Attention model exhibits excellent performance and robustness in stock price prediction.

关键词： Stock price prediction generative adversarial network attention mechanism time-series prediction

Flow field distribution and overpressure characteristics inside the crew compartment of a truck-mounted howitzer under the effect of muzzle blast

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Defence Technology(防务技术) 2025年第2期44卷 190-205页

作者： Shengcheng wei Linfang Qian Yadong Xu Qiang yin Xinyu Xiong School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing210094China Northwest Institute of Mechanical and Electrical Engineering Xianyang712099China

The muzzle blast overpressure induces disturbances in the flow field inside the crew compartment(FFICC)of a truck-mounted howitzer during the artillery *** overpressure is the primary factor preventing personnel from firing artillery within the *** investigate the overpressure characteristics of the FFICC,a foreign trade equipment model was used as the research object,and a numerical model was established to analyze the propagation of muzzle blast from the muzzle to the interior of the crew compartment under extreme firing *** comparative verification,the muzzle blast experiment included overpressure data from both the flow field outside the crew compartment(FFOCC)and the FFICC,as well as the acceleration data of the crew compartment structure(Str-CC).The research findings demonstrate that the overpressure-time curves of the FFICC exhibit multi-peak characteristics,while the pressure wave shows no significant *** enclosed nature of the cab hinders the dissipation of pressure wave energy within the FFICC,leading to sustained high-amplitude *** frameskin structure helps attenuate the impact of muzzle blast on the ***,local high overpressure caused by the convex or concave features of the cab's exterior significantly amplifies the overpressure amplitude within the FFICC.

关键词： Truck-mounted howitzer Muzzle blast Flow field inside the crew compartment(FFICC) Overpressure characteristics Flow-structure interaction

Prospects on mangrove knowledge services in the smart era:From plant atlas to knowledge graphs

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Science China Earth Sciences 2025年第1期68卷 111-127页

作者： Zhi-wei HOU Wenlong JING Cheng-Zhi QIN Ji YANG Qing XIA Xiaoling yin Key Laboratory of Guangdong for Utilization of Remote Sensing and Geographical Information System Guangdong Open Laboratory of Geospatial Information Technology and ApplicationGuangzhou Institute of GeographyGuangdong Academy of SciencesGuangzhou510070China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou511458China State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing100049China School of Traffic&Transportation Engineering Changsha University of Science and TechnologyChangsha410114China

Mangroves are crucial to the ecological security of the Earth and human *** management,conservation,and restoration are of great importance and necessitate the support of spatio-temporal information and multidisciplinary knowledge such as biology and *** knowledge services such as plant atlas provide illustrated textual knowledge of ***,this kind of service is oriented to information retrieval and is incapable of effectively mining and utilizing fragmented knowledge from multi-source heterogeneous data,facing the problem of“massive data,rare knowledge”.Knowledge graphs are capable of extracting,organizing,and fusing the knowledge contained in massive data into semantic networks that can be understood and computed by *** provide a solution for the realization of intelligent knowledge *** on the urgent need for mangrove knowledge acquisition,formal representation,and intelligent services,this paper proposes a research prospect on mangrove knowledge graphs and knowledge *** first analyze the similarities and differences between various domain-specific concepts of *** this basis,we define the mangrove knowledge graph as a large-scale knowledge base that integrates multi-disciplinary knowledge and spatio-temporal information with mangrove ecosystems as the ***,we propose a research framework for mangrove knowledge services that can realize the transformation from multi-modal data to intelligent knowledge services,including multiple research levels such as ubiquitous data sensing and aggregation,knowledge organization and graph construction,and intelligent mangrove knowledge ***,the methods and workflow for constructing mangrove knowledge graphs are ***,we discuss the challenges and possible future directions of mangrove knowledge services in the smart era,including the construction of a mangrove knowledge system that integrates the domain-specific charac

关键词： Mangrove Tupu Knowledge graph Multi-modal Knowledge service

Discovery of a new family member of arsenic–vanadium polyoxometalates with high catalytic performance for one-pot coupling of CO2, epoxides and amines

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Tungsten 2025年第1期7卷 80-87页

作者： Fang-Qian Wang yin Zhang wei-wei Wang Mei-Qian Fu Hong-Bing Mo wei-Dong Yu Chao Liu Jun Yan College of Chemistry and Chemical Engineering Central South University Junior Education Department Changsha Normal University School of Science Hunan University of Technology and Business

Arsenic-vanadium polyoxometalates（POMs） represent a family of tremendous potential catalysts owing to their peculiar physical and chemical *** in CO2conversion,using POMs to catalyze the conversion of CO2and epoxide into chemical intermediates is the research ***,A new family number of arsenic-vanadium POMs,[N（CH3）4]4[As8V12O38（H2O）]·2H2O({As8V12O38}),was reported and characterized by single-crystal X-ray diffraction,fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,*** from other allotropicity,{As8V12O38}shows a different structure pattern with an I-43d *** obtained {As8V12O38} was employed as a catalyst for the *** initial catalytic reaction confirmed that {As8V12O38} can catalyze the formation of epoxy carbonate by using 1.01 kPa CO2and epoxide at 70℃.Furtherly,when added amine in the catalytic system,oxazolidinone could be obtained by high yields,which provides an efficient way to convert CO2into profitable ***,a possible catalytic reaction mechanism was proposed by using different characterization analyses.{As8V12O38} first catalyzes the epoxide and CO2to form an epoxy carbonate and then catalyzes epoxy carbonate and benzylamine into oxazolidinone.

关键词： Arsenic CO2 conversion Oxazolidinone Polyoxometalates Vanadium

Vibration safety assessment and parameter analysis of buried oil pipelines based on vibration isolation holes under strong surface impact

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Earthquake Engineering and Engineering Vibration 2025年第1期24卷 69-82页

作者： Wang Guobo Mei Hua Wang Jianning He wei yin Yao Zhai Yuxin Zuo Pengfei College of Civil Engineering and Architecture Wenzhou UniversityWenzhou 325035China Zhejiang Engineering Research Center of Disaster Prevention and Mitigation for Coastal Soft Soil Foundation WenZhou 325035China China National Machinery Industry Co. Ltd.Beijing 100080China Faculty of Engineering China University of GeosciencesWuhan 430074China China Railway Construction Group Co. Ltd.Beijing 100072China School of Civil Engineering and Architecture Wuhan University of TechnologyWuhan 430070China

Strong surface impact will produce strong vibration,which will pose a threat to the safety of nearby buried pipelines and other important lifeline *** on the verified numerical method,a comprehensive numerical parameter analysis is conducted on the key influencing factors of the vibration isolation hole(VIH),which include hole diameter,hole net spacing,hole depth,hole number,hole arrangement,and soil *** results indicate that a smaller ratio of net spacing to hole diameter,the deeper the hole,the multi-row hole,the hole adoption of staggered arrangements,and better site soil conditions can enhance the efficiency of the VIH *** average maximum vibration reduction efficiency within the vibration isolation area can reach 42.2%.The vibration safety of adjacent oil pipelines during a dynamic compaction projection was evaluated according to existing standards,and the measurement of the VIH was recommended to reduce excessive *** single-row vibration isolation scheme and three-row staggered arrangement with the same hole parameters are suggested according to different *** research findings can serve as a reference for the vibration safety analysis,assessment,and control of adjacent underground facilities under the influence of strong surface impact loads.

关键词： vibration isolation hole buried oil pipeline strong surface impact vibration velocity vibration safety assessment