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Cloning and Expression of One Fibrinolytic Enzyme from Bacillus sp. zlw-2

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Agricultural Sciences in China 2009年第5期8卷 591-596页

作者： HAN Xin-mian GUO Run-fang YU hong-wei JIA ying-min Department of Biology Engineering Agricultural University of Hebei Baoding 071000 P.R.China

The gene encoding fibrinolytic enzyme from Bacillus sp. zlw-2 was cloned and sequenced （accession no. EU734749）, which was 1146 bp, encoded 381 amino acids and had 99% homology with Nattokinase YF308 and NAT. The genes encoding pre-pro-fibrinolytic enzyme （including signal peptide, propeptide, and mature peptide） and fibrinolytic enzyme （including mature peptide） were cloned into pET28a vector respectively and then transformed into Escherichia coli BL21 （DE3）. The recombinant ofpre-pro-fibrinolytic enzyme showed enzyme activity of 183 U mL^-1, while no detectable enzyme activity could be found from the recombinant of the mature peptide.

关键词： fibrinolytic enzyme Nattokinase clone recombinant expression

Cloning and Sequence Analysis of a Novel Cold-Adapted Lipase Gene from Strain lip35 (Pseudomonas sp.)

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Agricultural Sciences in China 2008年第10期7卷 1216-1221页

作者： WANG Cai-hong GUO Run-fang YU hong-wei JIA ying-min Department of Biology Engineering Agricultural University of Hebei Baoding 071000 P.R.China

A combination method of the usual-PCR and reverse-PCR for the cloning of a novel lipase gene directly from the total genomic DNA of strain lip35 （Pseudomonas sp.） is described, whereby a lipase gene （lip） was cloned directly from genomic DNA. The sequence data have been deposited in the GenBank and EMBL data bank with the accession number EU414288. The nucleotide sequence showed a major open reading frame encoding a 59-kDa protein of 566 amino acid residues, which contained a lipase consensus sequence GXSXG. The lipase lip had 74 and 70% homologies with the lipases of an uncultured bacterium and P. fluorescens PfO-1, respectively, but it did not show any overall homology with lipases from other origins. The functional lipase was obtained when the lip gene was expressed in Pichia pastoris GS115.

关键词： PCR reverse-PCR lipase gene clone sequence analysis

Photo-promoted carbonylation of chloroalkanes with carbon monoxide by non-precious metal catalysts

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Chinese Chemical Letters 2007年第7期18卷 795-798页

作者： hong wei Wang ying Ping Jia Da Bin Gao Jing Mei Yin Guang-Yun-Zhou Shen Min Li Liaoning Key Laboratory of Bio-Organic Chemistry Dalian University Dalian 116622 China

The photo-promoted carbonylation of chloroalkanes with carbon monoxide was carried out under ambient conditions with copper and cadmium salts catalysts. The results indicated that the corresponding esters were produced with three salt catalysts, ***2, CuCl2 and CdI2. Among these catalysts, CdI2 was the most efficient in terms of ester yield and selectivity, particularly, 60% yield and 75% selectivity in the carbonylation of chlorocyclohexane were achieved. Furthermore, the yield and selectivity of the ester can be improved greatly by adding tri-n-butylamine in the CuBr2 and CuCl2 catalyst systems. On the other hand, the additive. As a result, we suggest that iodide ion plays an important role in the catalyst system of the cadmium salts.

关键词： Cadmium salts Copper salts Chloroalkanes Photo-promoted carbonylation

Research on the Blocking Mechanism of the Transformation from Group Unemployment to Mass Incidents in China

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Research on the Blocking Mechanism of the Transformation fro...

Malignant fibrous histiocytoma of right atrium

2012年公共管理国际会议

作者： wei hong-ying YANG Cong-kun LI Shi-yong Shenzhen Polytechnic

ISBN: (纸本)9787564710316

The self-burning event of a Tunisian unemployed college student incurred a political tsunami in Arabian world, which is a wake-up call to various countries’ governmental administration. The selection of this topic has big significance. Applying the theoretical framework of government risk management, and based on the forming mechanism of mass incidents, this article carries out research on the blocking mechanism of the transformation from group unemployment to mass incidents in China. This article considers that the blocking mechanism consists of three processes blocking mechanism of management, prevention and control, and also consists of key elements intervention mechanism of psychology, information and public voice aroused by unemployment. Therefore, what need to be established and completed includes the following elements. The first is mass incidents management and employment promotion mechanism. The transformation from unemployment to big mass incidents is rooted in the group unemployment. We need to ascertain the measurable standard of mass unemployment and the critical point of survival pressure of the unemployed group, and then provide our support to them. The second is the monitoring and early warning mechanism of group unemployment risk dominated by government, including risk identification, alert source, alert rank and alert. The third is organization integration mechanism and information sharing mechanism.

关键词： Group unemployment Mass incidents Risk management Processes blocking mechanism of management Elements intervention mechanism

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Chinese Medical Journal 2013年第10期126卷 1994-1995页

作者： CHEN Huai-sheng WANG wei hong Cheng-ying Intensive Care Unit Second Affiliated Hospital of Jinan UniversityShenzhen People's Hospital Shenzhen Guangdong 518020 China

Afifty-year-old male patient was admitted with dyspnoea and cough. Four days before admission, he wasepisode of dyspnoea, could not prostrate with non- productive cough when he was offered with a gastroscopy examination in a local hospital due to anepithymia. The patient had no history of medication or allergies. However, he had smoked for more than thirty years-sixty cigarettes per day. Chest distress without haemoptysis, pink frothy sputum, apsychia, amaurosis, and fever was developed. The symptoms worsened gradually. On examination at the Department of Emergency, Shenzhen People＇s Hospital, dyspnoea with jugular vein distension and oedema in his head, neck and thoracic region were discovered. The breathing sounds on both lower lungs were low. His heart rate was 100 beats/min without arrhythmia or murmur. His abdomen was soft without rebound tenderness. His liver and spleen were not palpable. Pitting oedema occurred in both low limbs. Furosemide was given, but the symptoms were not relieved.

关键词： cardiac malignant fibrous histiocytoma heart failure metastases

Evaluation of dry eye disease symptomatology and mental health status among patients with different COVID-19 statuses

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International Journal of Ophthalmology(English edition) 2024年第5期17卷 822-830页

作者： Fang Ruan Wen-Jun Kong Qian Fan hong-wei Dong wei Zhang Wen-Bin wei ying Jie Department of Ophthalmology Beijing You’an HospitalCapital Medical UniversityBeijing Infectious Eye Disease Diagnosis and Treatment CenterBeijing 100069China Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Institute of OphthalmologyBeijing Ophthalmology and Visual Sciences Key LabBeijing 100730China Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual ScienceTianjin Eye InstituteNankai University Affiliated Eye HospitalNankai University Eye InstituteNankai UniversityClinical College of OphthalmologyTianjin Medical UniversityTianjin 300020China

AIM:To evaluate dry eye disease(DED)symptomatology and mental health status in different COVID-19 ***:A cross-sectional observational design was *** 123 eligible adults(46.34%of men,age range,18-59y)with COVID-19 included in the study from August to November,*** Surface Disease Index(OSDI),Five-item Dry Eye Questionnaire(DEQ-5),Hospital Anxiety and Depression Scale(HADS),and Pittsburgh Sleep Quality Index(PSQI)were used in this ***:OSDI scores were 6.82(1.25,15.91)in asymptomatic carriers,7.35(2.50,18.38)in mild cases,and 16.67(4.43,28.04)in recurrent cases,with 30.00%,35.56%,and 57.89%,respectively evaluated as having DED symptoms(χ2=7.049,P=0.029).DEQ-5 score varied from 2.00(0,6.00)in asymptomatic carriers,3.00(0,8.00)in mild cases,and 8.00(5.00,10.00)in recurrent cases,with 27.50%,33.33%,and 55.26%,respectively assessed as having DED symptoms(χ2=8.532,P=0.014).The prevalence of clinical anxiety(50.00%)and depression(47.37%)symptoms were also significantly higher in patients with recurrent infection(χ2=24.541,P<0.001;χ2=30.871,P<0.001).Recurrent infection was a risk factor for high OSDI scores[odds ratio,2.562;95%confidence interval(CI),1.631-7.979;P=0.033]and DEQ-5 scores(odds ratio,3.353;95%CI,1.038-8.834;P=0.043),whereas having a fixed occupation was a protective factor for OSDI scores(odds ratio,0.088;95%CI,0.022-0.360;P=0.001)and DEQ-5 scores(odds ratio,0.126;95%CI,0.039-0.405;P=0.001).CONCLUSION:Patients with recurrent COVID-19 have more severe symptoms of DED,anxiety,and depression.

关键词： COVID-19 dry eye disease recurrent infection mental health

Malvidin Mitigates Sepsis-induced Cardiac Injury by Modulating the TLR4-iNOS-COX-2 Inflammatory Pathway and the Bax/Bcl-2/Cyto-C Mitochondrial Apoptosis Pathway in a p38 MAPK-dependent Manner

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Biomedical and Environmental Sciences 2024年第2期37卷 221-227页

作者： ZHANG wei YUAN Si Long QIANG Jing Chao HUANG He LI Da SUN ying ZHANG hong Gang Department of Vascular Surgery The First People’s Hospital of LianyungangLianyungang 222005JiangsuChina Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening College of PharmacyJiangsu Ocean UniversityLianyungang 222005JiangsuChina Jinzhou Medical University postgraduate training base(Lianyungang First People’s Hospital) Lianyungang 222005JiangsuChina

In critical care medicine,sepsis is a dangerous systemic condition that is highly prevalent and is associated with high morbidity and mortality rates^([1]).The high mortality rate associated with sepsis is closely related to multi-organ dysfunction,with heart injury being particularly critical and considered the starting point of multi-organ injury^([2]).

关键词： mortality Apoptosis TLR4

Nutrient transport following water transfer through the world's largest water diversion channel

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Journal of Environmental Sciences 2024年第1期135卷 703-714页

作者： Yuanhui Cheng hong Zhang wei Yin State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085 China Changjiang Water Resources Protection Institute Wuhan 430051China University of Chinese Academy of Sciences Beijing 100049China

Nutrient levels in the artificial channel constructed for the Middle Route Project are significant indicators of water quality safety and aquatic ecological integrity for this large,interbasin ***,the distribution and transport of nutrients along the channel were poorly *** on a time-series dataset as well as mass balance and material flow analysis methods,the water and nutrient transport fluxes in the Middle Route of the South-to-North Water Diversion Project were identified in this *** results indicate that the nutrient concentrations varied considerably with time,but there was no significant difference among the 30 stations of the main *** temperature difference was the major factor in the large fluctuations of water quality indicators over *** nutrient loadings varied with the water volume outputs from the main channel to the waterreceiving *** deposition was an important source of nutrients in the main channel,accounting for 9.13%,20.6%,and 0.635%of the nitrogen,phosphorus,and sulfur input from the Danjiangkou Reservoir,*** 2021,a net accumulation of 988 tons of N,29 tons of P,and 2,540 tons of S,respectively,were present in the main *** increase of these external and internal nutrient loadings would cause water quality fluctuation and deterioration in some local sections of the main *** study quantified the spatial and temporal patterns of nutrient transport in the Middle Route and revealed the ecological effects on the aquatic environment,assisting authorities on the project to develop effective water conservation strategies.

关键词： Nutrient transport Spatiotemporal patterns The Middle Route The South-to-North Diversion Project Hydro-ecological effects Water-receiving cities

The current situation of semen analysis in China,based on a survey of 296 laboratories

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Asian Journal of Andrology 2024年第2期26卷 220-221页

作者： Long-Long Fu ying Liu Xiao-wei Wang Fang Zhou ying Guo Wen-hong Lu Yi-Qun Gu National Health Commission Key Laboratory of Male Reproductive Health Reproductive Health Research CenterNational Research Institute for Family PlanningBeijing 100081China Institute of Pediatric Research Children's Hospitalof Soochow UniversitySuzhou 215025China

Dear Editor,Semen analysis is a basic test to assess male fertility and is a fundamental test in assisted reproductive *** the first publication of World Health Organization(WHO)Laboratory Manual of Human Semen and Sperm-Cervical Mucus Interaction in 1980,to the publication of the WHO Manual,6th edition in 2021,these 40 years have been recognized as providing a global standard that is widely used in research and clinical laboratories *** WHO Manual,6th edition in Chinese,which we have been authorized by the WHO to translate,will be published soon.

关键词： technology. analysis translate