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Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens

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ANIMAL NUTRITION 2024年第3期18卷 72-83页

作者： Huang, Zhenwu Dai, Hongjian Li, Simeng Wang, Zhe wei, quanwei Ning, Zhonghua Guo, Yuming Shi, Fangxiong Lv, Zengpeng China Agr Univ Coll Anim Sci & Technol State Key Lab Anim Nutr & Feeding Beijing 100193 Peoples R China Nanjing Agr Univ Coll Anim Sci & Technol Nanjing 210095 Peoples R China Chinese Acad Agr Sci Inst Anim Sci State Key Lab Anim Nutr & Feeding Beijing 100193 Peoples R China Aksu Vocat Tech Coll Sch Bioengn Aksu 843000 Peoples R China

The development of skeletal muscle is a crucial factor in determining the meat yield and economic benefits of broiler production. Recent research has shown that mulberry leaves and their extracts can be used to significantly improve the growth performance of livestock and poultry. The present study aims to elucidate the mechanisms involved in the regulation of skeletal muscle development in broiler offspring by dietary mulberry-leaf flavonoids (MLF) supplementation from the perspective of maternal effect theory. A total of 270 Qiling broiler breeder hens were randomly assigned to 3 treatments with different doses of MLF (0, 30, 60 mg/kg) for 8 weeks before collecting their fertilized eggs. The chicken offspring at 13 and 19 d of embryonic stage, and from 1 to 28 d old after hatching were included in this study. The results showed that maternal supplementation increased the breast muscle weight and body weight of the offspring at the embryo and chick stages (P < 0.05). This was followed by increased cross-sectional area of pectoral muscle fibres at 14 d (P < 0.05). Further determination revealed a tendency towards increased serum levels of insulin-like growth factor 1 (IGF-1) (P = 0.092) and muscle fibre count (P = 0.167) at 1 d post-hatching following maternal MLF treatment, while serum uric acid (UA) was decreased at 14 d after hatching (P < 0.05). Moreover, maternal MLF supplementation significantly up-regulated the mRNA expression of the myogenic regulatory factor Myf5 in skeletal muscle at the both embryonic and growth stages (P < 0.05). The relative abundance of the downstream protein of BMPR2, Smad1 and p-Smad1/5/9 in the TGF beta signalling pathway was significantly increased by maternal MLF treatment. Meanwhile, the increased expression of the target protein p-mTOR in the breast muscle of the offspring chicks is in accordance with the improved growth rate of the breast and the body. In conclusion, maternal MLF supplementation can promote muscle protein metab

关键词： Broiler breeder hen Mulberry-leaf flavonoids Offspring Protein metabolism Skeletal muscle GROWTH-PERFORMANCE IMMUNE FUNCTION HYPERTROPHY LEAVES MECHANISMS REGULATOR PATHWAY EMBRYO DIETS BONE

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Assessment of spermatogenesis in camels via seminiferous tubule staging and testosterone profiling

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Asian pacific Journal of Reproduction 2024年第3期13卷 133-142页

作者： Saif Ullah Abdul Quddus quanwei wei Wael Ennab Sheeraz Mustafa Jiahao Sun Rahmani Mohammad Malyar Abdul Baseer Dagan Mao Fangxiong Shi College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China Faculty of Veterinary and Animal Sciences Lasbela University of Agriculture Water and Marine SciencesBalochistanPakistan Faculty of Veterinary and Animal Sciences Ziaddin University Karachi Pakistan

Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel ***:Testicular tissue samples were obtained from 12 dromedary bull camels aged 7 to 8 at a local *** histological assessment involved tissue processing,hematoxylin and eosin(H&E)staining,and examination under a *** analysis,germ cell identification,and assessment of seminiferous tubules and maturation were *** assay was performed by radioimmunoassay using blood samples collected at regular ***:The study revealed 12 phases of the dromedary camel's seminiferous epithelium cycle,highlighting distinct morphological characteristics and cellular *** migration,maturation,cap formation,and the Golgi-mediated synthesis of proacrosomal vesicles were also explained in dimension,as were the steps of acrosome *** and mature sperm cells were present when spermatogenesis phases were *** analysis of the dimensions of seminiferous tubules revealed specific measures for diameter,area,and epithelial height about luminal ***,there were noticeable variations in the serum testosterone concentrations during the study period,indicating temporal ***:This study outlines the spermatogenesis process in dromedary camels across 12 stages,emphasizing cellular dynamics and acrosomal *** also provides seminiferous tubule measurements and observes seasonal testosterone fluctuations,offering insights into reproductive regulation and potential strategies for camel breeding conservation.

关键词： Spermatogenesis Camel Testosterone profile Morphological staging

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Single channel signal component separation using Bayesian estimation

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Journal of Systems Engineering and Electronics 2007年第1期18卷 33-39页

作者： Cai quanwei wei Ping Xiao Xianci Coll. of Electronic Engineering Univ. of Electronic Science and Technology Chengdu 610054 P.R. China

A Bayesian estimation method to separate multicomponent signals with single channel observation is presented in this paper. By using the basis function projection, the component separation becomes a problem of limited parameter estimation. Then, a Bayesian model for estimating parameters is set up. The reversible jump MCMC （Monte Carlo Markov Chain） algorithmis adopted to perform the Bayesian computation. The method can jointly estimate the parameters of each component and the component number. Simulation results demonstrate that the method has low SNR threshold and better performance.

关键词： Signal component separation Single channel Bayesian estimation Reversible jump MCMC

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Maternal stevioside supplementation improves intestinal immune function of chicken offspring potentially via modulating gut microbiota and down-regulating the promoter methylation level of suppressor of cytokine signaling 1(SOCS1)

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Animal Nutrition 2022年第3期10卷 329-346页

作者： Jingle Jiang Lina Qi quanwei wei Fangxiong Shi College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China

The intestinal immune function of chickens is limited during the early growing *** nutritional intervention has been suggested to affect the innate immunity of *** present study aimed to investigate the effects of maternal stevioside supplementation on the intestinal immune function of chicken offspring.A total of 120 Jinmao yellow-feathered breeder hens were fed a basal diet or a diet supplemented with 250 mg/kg stevioside for 5 *** the last week,200 breeding eggs from each group were collected for *** hatching,80 male offspring(40 chickens from each group)were randomly selected and fed the same basal diet for 28 *** addition,90 well-shaped fertile eggs of non-treated breeder hens were incubated for the in ovo injection *** dissolved in 20%glycerol was injected at 7 d of *** results showed that maternal stevioside supplementation could improve embryonic development,jejunal integrity and proliferation in the jejunal crypt(P0.05).Furthermore,maternal stevioside supplementation could induce hypo-methylation on the promoter region of suppressor of cytokine signaling 1(SOCS1).In conclusion,maternal stevioside supplementation could improve the intestinal immune function of chicken offspring potentially via modulating the gut microbiota and downregulating the promoter meth

关键词： Maternal Stevioside Jejunum Transcriptome Offspring Immune function

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A blind separation method of overlapped multi-components based on time varying AR model

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Science in China(Series F) 2008年第1期51卷 81-92页

作者： CAI quanwei wei Ping XlAO XianCi College of Electronic Engineering University of Electronic Science and Technology of China Chengdu 610054 China

A method utilizing single channel recordings to blindly separate the multicomponents overlapped in time and frequency domains is proposed in this paper. Based on the time varying AR model, the instantaneous frequency and amplitude of each signal component are estimated respectively, thus the signal component separation is achieved. By using prolate spheroidal sequence as basis functions to expand the time varying parameters of the AR model, the method turns the problem of linear time varying parameters estimation to a linear time invariant parameter estimation problem, then the parameters are estimated by a recursive algorithm. The computation of this method is simple, and no prior knowledge of the signals is needed. Simulation results demonstrate validity and excellent performance of this method.

关键词： time varying AR model time and frequency domains overlap single channel multi-components separation recursive algorithm

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Comparability in geomorphic evolution of the three rivers in the northern Longitudinal Range-Gorge Region： Evidence of statistic relationships between geomorphic parameters and analyses of cause of formation

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Chinese Science Bulletin 2006年第B11期51卷 39-49页

作者： WANG Suiji WU Shaohong HE Daming DAI Erfu ZHOU Changhai wei quanwei Key Laboratory of Water Cycle and Related Land Surface Processes Institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China College of Life Science Yunnan University Kunming 650091 China

For discussing the evolution characteristics and correlation of the tributaries of the Nujiang, Lancang and Jinsha rivers in the northern longitudinal rang-gorge region of northwestern Yunnan, the relationships between some geomorphic parameters of the total, first- and second-order tributaries whose catchment areas are larger than 100 km(2) in the three rivers of the study area are analyzed and a series of correlation formulae are proposed. Results indicate that the corresponding order tributary systems of the three rivers have very high comparability especially for the first-order tributaries. The statistical relationships between catchment area and channel length of the corresponding order tributary systems indicate that the Nujiang and Lancang rivers have more commonalities than the Jinsha River, being very evident in the relationships between the total- and first-order tributaries. The relationships between catchment area and channel gradient also indicate that there is more high comparability between the Nujiang and Lancang rivers, being very evident in the relationships of the total-order tributaries. The causes leading to the high comparability between the three rivers are as follows. The geological tectonic comparability in the longitudinal rang-gorge region causes the comparability of landform and the comparability of land surface condition, thus causing the comparability of the geomorphic evolution in the study area. The Nujiang and Lancang rivers in the study area are located at the margin of Eurasia Plate. They are strongly controlled by the collision zone of the India and Eurasia plates. The Jinsha River in the study area is relatively far from the collision zone. It is controlled by the collision zone in relative low degree and its geomorphic evolution is relatively free. Furthermore, relative infirm runoff because of the relative arid climate in the Jinsha River reach led to the relatively low ability in alteration of the landform systems of the rive

关键词：怒江澜沧江金沙江统计关系云南西北水文学

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Single-channel blind separation of overlapped multicomponents based on energy operator

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Science China(Information Sciences) 2010年第1期53卷 147-157页

作者： CAI quanwei, wei Ping & XIAO XianCi College of Electronic Engineering, University of Electronic Science and Technology of China, Chengdu 610054, China College of Electronic Engineering University of Electronic Science and Technology of China

A method utilizing single channel recordings to blind separate the multicomponents overlapped in both time and frequency domains is proposed in this paper. The main idea of the method is based on using the energy operator and differential energy operator to estimate instantaneous frequencies and amplitudes of each component and then to reconstruct these components. This method, due to the good time resolution of the energy operator and differential energy operator, as well as the very low computational complexity, is very useful in real time processing and practical applications. Simulation results demonstrated the effciency of this method.

关键词： single channel time frequency overlap multicomponents separation energy operator differential energy operator

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新农科背景下农业高校专业课程产教融合建设实践与思考——以南京农业大学动物繁殖学课程为例

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黑龙江动物繁殖 2024年第2期32卷 50-53页

作者：茆达干王子玉魏全伟万永杰张艳丽杭苏琴王锋南京农业大学南京210095

高等学校新农科专业建设旨在培养知农爱农的新型农业技术人才,产教融合在教育教学、人才培养、产业发展和创新创业等方面发挥重要作用。动物繁殖学是动物科学专业必修课程,主要研究动物生殖规律和技术,内容更新快,实践性强,是学习动物... 详细信息

高等学校新农科专业建设旨在培养知农爱农的新型农业技术人才,产教融合在教育教学、人才培养、产业发展和创新创业等方面发挥重要作用。动物繁殖学是动物科学专业必修课程,主要研究动物生殖规律和技术,内容更新快,实践性强,是学习动物生产学各论的基础课程,在专业人才培养中占有重要地位。文章以南京农业大学国家一流课程动物繁殖学为例,探讨了产教融合建设的必要性,重设教学目标、重建教学体系、优化考核方式的实践成效,在此基础上创新课程改革思路,以期为我国高等农业院校专业课程建设提供借鉴。

关键词：新农科产教融合教学改革专业课程动物繁殖学

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A Scoping Review of Real-World Study in Vaccine Evaluation

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China CDC weekly 2024年第16期6卷 357-362页

作者： Lei Wang Hong Yang Lanfang Xia quanwei Song Na Liu Guomin Zhang Fuzhen Wang Huaqing Wang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases(NITFID) BeijingChina National Immunization Program Chinese Center for Disease Control and PreventionBeijingChina

Real-world study(RWS)gained prominence starting with a significant investigation on ramipril’s impact on hypertension in 1993(1).A key advancement for the Food and Drug Administration(FDA)occurred with the enactment of the 21st Century Cures Act in 2016,emphasizing RWS(2).The FDA’s Real-world Evidence Program establishes real-world data(RWD)as“data relating to patient health status and/or the delivery of health care routinely collected from a variety of sources”,while real-world evidence(RWE)is“clinical evidence about the usage and potential benefits or risks of a medical product derived from analysis of RWD”(3).The proliferation of big data,electronic medical records(EMR),electronic health records(EHR),and medical claims data offers vast information resources facilitating *** access to RWD allows for worldwide monitoring of the impacts of various public health initiatives like vaccination *** randomized clinical trials(RCTs)traditionally gauge vaccine efficacy and shortterm safety,RWS can assess vaccine performance and safety across larger,more diverse populations and are adept at identifying rare events not easily discernible in RCTs due to their infrequency.

关键词： routine gained traditionally

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Comparison of Statistical Signal Detection Methods in Adverse Events Following Immunization — China, 2011–2015

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China CDC weekly 2024年第16期6卷 350-356,I0001页

作者： Lanfang Xia Keli Li Yan Li Zhijie An quanwei Song Lei Wang Zundong Yin Huaqing Wang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases(NITFID) BeijingChina National Immunization Program Chinese Center for Disease Control and PreventionBeijingChina

Introduction:The current study aims to assess the performance of data mining techniques in detecting safety signals for adverse events following immunization(AEFI)using routinely obtained data in *** different methods for detecting vaccine safety signals were ***:The AEFI data from 2011 to 2015 was collected for our *** analyzed the data using four different methods to detect signals:the proportional reporting ratio(PRR),reporting odds ratio(ROR),Bayesian confidence propagation neural network(BCPNN),and multi-item gamma Poisson shrinker(MGPS).Each method was evaluated at 1–3 thresholds for *** assess the performance of these methods,we used the published signal rates as gold standards to determine the sensitivity and ***:The number of identified signals varied from 602 for PRR1(with a threshold of 1)to 127 for *** considering the common reactions as the reference standard,the sensitivity ranged from 0.9%for MGPS1/2 to 38.2%for PRR1/2,and the specificity ranged from 85.2%for PRR1 and ROR1 to 96.7%for *** considering the rare reactions as the reference standard,PRR1,PRR2,ROR1,ROR2,and BCPNN exhibited the highest sensitivity(73.3%),while MGPS1 exhibited the highest specificity(96.9%).Discussion:For common reactions,the sensitivities were modest and the specificities were *** rare reactions,both the sensitivities and specificities were *** study provides valuable insights into the selection of signal detection methods and thresholds for AEFI data in China.

关键词： specificity routine vaccine

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