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The role of viral protein Ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex

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Virologica Sinica 2016年第6期31卷 480-489页

作者： Jingfang Mu Yongli Zhang Yangyang hu Xue hu Yuan Zhou Xinwen Chen yun wang State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China University of Chinese Academy of Sciences Beijing 100049 China

The actin nucleator actin-related protein complex(Arp2/3) is composed of seven subunits: Arp2,Arp3, p40/ARPC1(P40), p34/ARPC2(P34), p21/ARPC3(P21), p20/ARPC4(P20), and p16/ARPC5(P16). Arp2/3 plays crucial roles in a variety of cellular activities through regulation of actin polymerization. Autographa californica multiple nucleopolyhedrovirus(Ac MNPV), one of the beststudied alphabaculoviruses, induces Arp2/3 nuclear relocation and mediates nuclear actin polymerization to assist in virus replication. We have demonstrated that Ac34, a viral late-gene product, induces translocation of the P40 subunit of Arp2/3 to the nucleus during Ac MNPV infection. However, it remains unknown whether Ac34 could relocate other Arp2/3 subunits to the nucleus. In this study, the effects of the viral protein Ac34 on the distribution of these subunits were studied by an immunofluorescence assay. Arp2, P34, P21, and P20 cloned from Spodoptera frugiperda(Sf9) cells showed mainly cytoplasmic localization and were relocated to the nucleus in the presence of Ac34. In addition, Arp3 was localized in the cytoplasm in both the presence and absence of Ac34, and P16 showed whole-cell localization. In contrast to Sf9 cells, all subunits of mammalian Arp2/3 showed no nuclear relocation in the presence of Ac34. Co-immunoprecipitation analysis of the interaction between Ac34 and Arp2/3 subunits revealed that Ac34 bound to P40,P34, and P20 of Sf9 cells. However, none of the subunits of mammalian Arp2/3 interacted with Ac34, indicating that protein-protein interaction is essential for Ac34 to relocate Arp2/3 subunits to the nucleus.

关键词： Autographa californica multiple nucleopolyhedrovirus(AcMNPV) Ac34 Arp2/3 complex nuclear relocation

Separability Criterion for Bipartite States and Its Generalization to Multipartite Systems

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Chinese Physics Letters 2014年第4期31卷 13-17页

作者： huANG Jie-hui hu Li-yun wang Lei Zhu Shi-Yao College of Physics and Communication Electronics Jiangxi Normal University Nanchang 330022 College of Physics Jilin University Changchun 130021 Beijing Computational Science Research Center Beijing 100084

A group of symmetric operators are introduced to carry out the separability criterion for bipartite and multipartite quantum states. All the symmetric operators, represented by a symmetric matrix with only two nonzero elements, and their arbitrary linear combinations are found to be entanglement witnesses. By using these symmetric operators, Wootters＇ separability criterion for two-qubit states can be generalized to bipartite and multipartite systems in arbitrary dimensions.

关键词： Systems Separability separability

Utilization of One Novel Microbial Esterase WDEst9 in the Kinetic Resolution of (5)-Methyl 2-chloropropionate and (5)-Ethyl 2-chloropropionate

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Chemical Research in Chinese Universities 2019年第5期35卷 830-836页

作者： wang Yilong XU Yongkai ZHANG yun SUN Aijun hu yunfeng CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301P.R.China Guangdong Key Laboratory of Marine Materia Medica South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301P.R.China Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan 250014P.R.China

One novel microbial esterase WDEst9 from Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085 was functionally characterized and the results demonstrated that the enantio-preference of WDEst9 was opposite to that of three other microbial esterases(BSE01701, PHE14 and Bae02030) in the kinetic resolution of racemic methyl lactate. We further investigated the potential of esterase WDEst9 in the kinetic resolution of both (±)-methyl 2-chloropropionate and (±)-ethyl 2-chioropropionate. The enantio-preference of WDEst9 was also interestingly opposite to that of esterases EST 12-7 and EstC10, and generated (S)-methyl 2-chloropropionate and (S)-ethyl 2-chloropropionate with high enantiomeric excess(both e.e.>98%) and high yield after many iterations of process optimization. Through genome mining, microbial esterase WDEst9 was characterized to be a novel esterase which may provide valuable complementary enantio-selectivity and possesses very good potential in the kinetic resolution of high value-added chiral chemicals.

关键词： Biocatalysis Novel esterase Asymmetric hydrolysis Complementary enantio-preference (5)-Methyl 2-chloropropionate (S)-Ethyl 2-chloropropionate

Numerical Study of the Intensity Correlation between Secondary Flow and Heat Transfer of Circle Tube-Finned Heat Exchanger with Vortex Generators

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Computer Modeling in Engineering & Sciences 2020年第4期123卷 237-256页

作者： Yong Guan Wanling hu yun Zhang Kewei Song Liangbi wang School of Environmental and Municipal Engineering Lanzhou Jiaotong UniversityLanzhouChina Key Laboratory of Railway Vehicle Thermal Engineering(Lanzhou Jiaotong University) Ministry of Education of ChinaLanzhouChina Department of Mechanical Engineering Lanzhou Jiaotong UniversityLanzhouChina

The application of vortex generators in tube-finned heat exchangers is very *** vortex generators can generate secondary flow,and as we all know secondary flow can obviously strengthen heat *** use vortex generators much more efficiently in the circle tube-finned heat exchangers,the intensity correlation study between secondary flow and heat transfer is needed.22 different structures of circle tube-finned heat exchangers were numerically studied,including the plain fin cases and the cases with vortex *** addition,the influence of fin spacing,transverse and longitudinal tube pitch,heights and attack angle of vortex generators,positions of vortex generators and shape of vortex generators on heat transfer and fluid flow are studied,*** non-dimensional parameter Se is applied to quantify the secondary flow *** results show that Se can describe the secondary flow intensity very *** is very close corresponding relationship between overall averaged Nu and volumetrically averaged Se for all the researched cases and the relational expression is ***,there is no one-to-one correlation not only between Re and f but also between volumetrically averaged Se and f for all the studied cases.

关键词： Enhanced heat transfer numerical analysis quantitative relationship secondary flow.

Versatile functionalization of amylopectin for effective biomedical applications

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Science China Chemistry 2015年第9期58卷 1461-1470页

作者： Ranran wang Hao hu Qin Cai Nana Zhao yun Zhu Fujian Xu State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Key Laboratory of Carbon Fiber and Functional Polymers Ministry of Education Beijing Laboratory of Biomedical Materials Chongqing Academy of Agricultural Science

Successful gene vectors should be with high transfection efficiency and minimal cytotoxicity. Natural polysaccharides, due to their good biocompatibility and biodegradability, have been widely studied and applied. Amylopectin is one of polysaccharides with dendritic structure and numerous hydroxyl groups that could be used for subsequent modification. In this work, a series of dendritic cationic gene vectors comprising amylopectin backbones and poly(2-(dimethylamino) ethyl methacrylate)(PDMAEMA) side chains with different lengths(termed as AMY-PDs) were readily prepared by atom transfer radical polymerization(ATRP). The gene condensation ability, cytotoxicity and gene transfection of AMY-PDs carriers were investigated. In comparison with "gold-standard" poly(ethyleneimine)(PEI, 25 k Da), the AMY-PDs exhibited higher transfection efficiency with lower cytotoxicity. AMY-PDs could be further modified with Au nanoparticles(termed as AMY-PD@Au). The potential of the AMY-PD@Au vectors to be utilized as a CT contrast agent for imaging of cancer cells was investigated. Such AMY-PD@Au vectors may realize gene therapy with the ability of real-time imaging.

关键词： amylopectin ATRP gene carriers CT imaging

基于SCIATRAN的临近空间氧气光谱敏感性分析

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光谱学与光谱分析 2025年第1期45卷 170-178页

作者：叶松胡双寒熊伟李树王新强王方原汪杰君桂林电子科技大学光电工程学院广西桂林541004 中国科学院安徽光学精密机械研究所中国科学院通用光学定标与表征技术重点实验室安徽合肥230031 广西光电信息处理重点实验室广西桂林541004

临近空间大气温度场的研究具有重要的科学意义,根据全时相大气温度示踪物辐射机制与分布特征研究表明,氧气作为大气的主要成分之一,广泛分布于临近空间且辐射强度明显,所以氧气探测是研究临近空间温度变化的重要基础。临近空间氧气探测... 详细信息

临近空间大气温度场的研究具有重要的科学意义,根据全时相大气温度示踪物辐射机制与分布特征研究表明,氧气作为大气的主要成分之一,广泛分布于临近空间且辐射强度明显,所以氧气探测是研究临近空间温度变化的重要基础。临近空间氧气探测主要是依据太阳辐射的短波红外部分被大气层氧气分子吸收得到的吸收谱线,该谱线携带着氧气含量等重要信息。利用探测器得到的氧气光谱进行高精度反演可以得到温度等信息。探测过程中的太阳天顶角、方位角、临边切高及气溶胶参数等因素对氧气影响不同,基于SCIATRAN模型分析氧气对不同参数的敏感性。通过对探测方式和环境因素引起的辐射亮度变化来分析计算氧气对其敏感性。结果表明:氧气的辐射亮度在天顶角为60°以下是随天顶角增大而增大的,但在超过60°后变化随之相反,一天内的太阳天顶角对于氧气的辐射亮度影响不超过0.01 W/m^(2)/nm/sr,且差值不超过7%;氧气辐亮度随方位角增大而减小;氧气辐射亮度随切线高增加而减小,且减小幅度逐渐下降;气溶胶类型对氧气辐射亮度的影响差值比例在10%以内;气溶胶季节对氧气辐射亮度影响是春夏季节要大于秋冬季节;不同气溶胶类型下的气溶胶光学厚度倍数对氧气辐射亮度影响的差值比例均在30%以内,且农村与海洋型气溶胶下光学厚度倍数与辐射亮度成正比,城市型气溶胶下与之相反。仿真数据的结果与实测数据结果对比分析验证了SCIATRAN模型仿真数据的有效性和可行性。该研究为临近空间的氧气探测提供了科学依据,为临近空间的反演等相关研究领域提供参考。

关键词：临近空间氧气辐射传输敏感性

Isomorphisms of fiber spaces over Teichmüller spaces

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Science China Mathematics 2006年第7期49卷 877-892页

作者： hu yun SHEN Yuliang Department of Mathematics Suzhou University Suzhou 215006 China Department of Mathematics Suzhou University Suzhou 215006 China

We will be mainly concerned with some important fiber spaces over Teichmuller spaces, including the Bers fiber space and Teichmuller curve, establishing an isomorphism theorem between 'punctured' Teichmuller curves an... 详细信息

关键词： Teichmuller space Bers fiber space 'punctured' fiber space Teichmuller curve 'punctured' Teichmuller curve biholomorphic isomorphism.

Numerical study on variation characteristics of the unsteady bearing forces of a propeller with an external transverse excitation

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Journal of Hydrodynamics 2019年第2期31卷 400-412页

作者： Min-hua Shu Ke Chen yun-xiang You Tian-qun hu Remfeng wang State Key Laboratory of Ocean Engineering Collaboration Innovation Center for Advanced Ship and Deep-Sea Exploitation EquipmentShanghai JiaoTong UniversityShanghai 200240China

The unsteady bearing forces of a propeller can be significantly affected by external transverse *** paper numerically studies the unsteady variation characteristics of the bearing forces of a three-blade conventional propeller with external transverse excitations or without *** dynamic mesh method combined with the RANS turbulence model is *** numerical simulation shows the frequency response in the frequency domain of KT,Kq under external transverse *** frequency of the response is found always equal to twice of the excitation *** the amplitude of the external transverse excitation keeps constant,the amplitude of the response increases with the in crease of the excitation *** the excitation frequency keeps constant,the amplitude of the response increases with the increase of the excitation amplitude,in a power-law *** pressure distribution can be significantly affected by the external transverse excitation,leading to a high irregularity of the flow field.

关键词： External transverse excitation unsteady bearing forces excitation frequency propeller dynamic mesh method

Influences of Mo on Nitrate Reductase, Glutamine Synthetase and Nitrogen Accumulation and Utilization in Mo-Efficient and Mo-Inefficient Winter Wheat Cultivars

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Agricultural Sciences in China 2010年第3期9卷 355-361页

作者： YU Min hu Cheng-xiao SUN Xue-cheng wang yun-hua Research Center of Trace Elements Huazhong Agricultural University Wuhan 430070 P.R. China Department of Horticulture Foshan University Foshan 528000 P.R. China

The objective is to study whether the accumulation and utilization of plant N are controlled by Mo status in winter wheat cultivars. Mo-efficient cultivar 97003 （eft） and Mo-inefficient cultivar 97014 （ineff） were grown in severely Mo-deficient acidic soil （Tamm-reagent-extractable Mo 0.112 mg kg^-1） with （＋Mo） and without （-Mo） the application of 0.13 mg kg^-1 Mo. The accumulation and use efficiency of plant total N were significantly higher in ＋Mo than that in -Mo and in eft than that in ineff under Mo deficiency. N use efficiency was remarkably higher in maturity but it was forwarded to jointing stage after Mo supply, thus indicating that Mo supply promoted the N use efficiency besides N uptake and eff was efficient in N uptake and utilization. The overall activity of nitrate reductase （NR, EC 1.***.6.1） was significantly higher in ＋Mo than in -Mo and ratio of ＋Mo/-Mo was even to 14.8 at filleting stage for ineff. Activity of glutamine synthetase （GS, EC 6.***.1.2） was significantly lower in ＋Mo than in -Mo. Concentration of nitrate and glutamate were also significantly lower in ＋Mo than in -Mo, thus provided evidences for enhancing N use efficiency by Mo supply. Activities of NR and GS were significantly higher and concentrations of nitrate and glutamate were significantly lower in eff than ineff under Mo deficiency, thus indicated eff was more efficient in N reduction and utilization. It is therefore concluded that Mo could promote N accumulation and utilization in winter wheat which was directly related to NR and feedback regulated by GS. Higher Mo status also results in higher accumulation and utilization of plant N in eft.

关键词： winter wheat Mo N accumulation N use efficiency nitrate reductase glutamine synthetase