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Photocatalytic degradation of L- acid by TiO_2 supported on the activated carbon

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Journal of Environmental Sciences 2006年第3期18卷 562-566页

作者： wang yu-ping wang Lian-jun PENG Pan-ying School of Chemical Engineering Nanjing University of Science and Technology Nanjing 210094China School of Chemistry and Environmental Science Nanjing Normal University Nanjing 210097 China

TiO2 sol was prepared by sol-gel technique with tetrabutyl titanate as precursor. Supported TiO2 catalysts on activated carbon were prepared by soak and sintering method. The aggregation of nano-TiO2 particles can be effectively suppressed by added polyethylene glycol （PEG） as a surface modifier. The average particle diameter of TiO2, specific surface area and absorbability of catalyst can be modified. Based on characteristics of the TiO2 photocatalyst with XRD, specific surface area, adsorption valves of methylene blue and the amount of TiO2 supported on the activated carbon, the photocatalytic degradation of L-acid was studied. The effect of the factors, such as pH of the solution, the initial concentration of L-acid on the photocatalytic degradation of L-acid, were studied also. It was found that when the pH of the solution is 1.95, the amount of photocatalyst is 0.5 g, the concentration of the L-acid solution is 1.34×10^3 mol/L and the illumination time is 7 h, the photocatalytic degradation efficiency of L-acid can reach 89,88%, The catalyst was reused 6 times and its degradation efficiency hardly changed.

关键词： L-acid activated carbon supported TiO2 photodegradation

ShRNA-mediated gene silencing of β-catenin inhibits growth of human colon cancer cells

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World Journal of Gastroenterology 2007年第48期13卷 6581-6587页

作者： Wen-Sheng Huang Jian-ping wang Ting wang Jie-yu Fang ping Lan Jin-ping Ma Surgery Department The First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 Guangdong Province China

AIM: To observe the gene silencing mediated by the specific shRNA targeted against β-catenin and its effect on cell proliferation and cycle distribution in the human colon cancer cell line Colo205. METHODS: Two shRNA plasmid vectors against β-catenin were constructed and transfected into Colo205 cells with LipofectamineTM2000. The down-regulations of β-catenin, c-myc and cyclinD1 expressions were detected by RT-PCR and western blot analysis. The cell proliferation inhibitions were determined by MTT assay and soft agar colony formation assay. The effect of these two β-catenin shRNAs on cell cycle distribution and apoptosis was examined by flow cytometry. RESULTS: These two shRNA vectors targeted against β-catenin efficiently suppressed the expression of β-catenin and its down stream genes, c-myc and cyclinD1. The expression inhibition rates were around 40%-50% either at the mRNA or at the protein level. The shRNA-mediated gene silencing of β-catenin resulted in significant inhibition of cell growth both on the culture plates and in the soft agar. Moreover, the cancer cells showed significant G0/G1 arrest and increased apoptosis at 72 h post transfection due to gene silencing. CONCLUSION: These specific shRNAs targeted against β-catenin could have a gene silencing effect and block the WNT signaling pathway. They could inhibit cell growth, increase apoptosis, and induce cell cycle arrest in Colo205 cells. ShRNA interference against β-catenin is of potential value in gene therapy of colon cancer.

关键词： β-catenin RNA interference Apoptosis Colon cancer WNT

Effective fraction from Simiao Wan prevents hepatic insulin resistant by inhibition of lipolysis via AMPK activation

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Chinese Journal of Natural Medicines 2022年第3期20卷 161-176页

作者： JIANG Qi-Xin CHEN Yi-Meng MA Jing-Jie wang yu-ping LI ping WEN Xiao-Dong YANG Jie State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China

Simiao Wan(SMW)is a traditional Chinese formula,including Atractylodis Rhizoma,Achyranthis Bidentatae Radix,Phellodendri Chinensis Cortex and Coicis Semen at the ratio of 1:1:2:*** can be used to the treatment of ***,its bioactive compounds and underlying mechanism are *** study was designed to screen the antilipolytic fraction from SMW and investigate its therapeutic mechanisms on hepatic insulin *** fractions of SMW were prepared by membrane separation combined with macroporous resin and their antilipolytic activity was screened in fasted *** effects of 60%ethanol elution(ESMW)on lipolysis were investigated in 3T3-L1 adipocytes stimulated by palmitic acid(PA)and high fat diet(HFD)-fed *** our study,ESMW was the bioactive fraction responsible for the antilipolytic activity of SMW and 13 compounds were characterized from ESMW by UHPLC-QTOF-MS/*** suppressed protein kinase A(PKA)-hormone-sensitive lipase(HSL)related lipolysis and increased AMP-activated protein kinase(AMPK)phosphorylation in PA challenged 3T3-L1 *** knockdown abolished the inhibitory effects of ESMW on IL-6 and HSL pSer-660,revealing that the antilipolytic and anti-inflammatory activities of ESMW are AMPK ***,ESMW ameliorated insulin resistance and suppressed lipolysis in HFD-fed *** inhibited diacylglycerol accumulation in the liver and inhibited hepatic *** medium collected from ESMW-treated 3T3-L1 cells ameliorated insulin action on hepatic gluconeogenesis in liver cells,demonstrating that the antilipolytic activity contributed to ESMW beneficial effects on hepatic glucose *** conclusion,ESMW,as the antilipolytic fraction of SMW,inhibited PKA-HSL related lipolysis by activating AMPK,inhibiting diacylglycerol(DAG)accumulation in the liver and thereby improving insulin resistance and hepatic gluconeogenesis.

关键词： Simiao Wan Insulin resistance Lipolysis AMPK

Effects of blueberry on hepatic fibrosis and transcription factor Nrf2 in rats

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World Journal of Gastroenterology 2010年第21期16卷 2657-2663页

作者： wang, yu-ping Cheng, Ming-Liang Zhang, Bao-Fang Mu, Mao Wu, Jun Guiyang Med Coll Dept Infect Dis Guiyang 550004 Guizhou Prov Peoples R China

AIM:To investigate the effects of blueberry on hepatic fibrosis and NF-E2-related factor 2(Nrf2) transcription factor in ***:Forty-five male Sprague-Dawley rats were randomly divided into control group(A);CCl4-induced hepatic fibrosis group(B);blueberry prevention group(C);Dan-shao-hua-xian capsule(DSHX) prevention group(D);and blueberry + DSHX prevention group(E).Liver fibrosis was induced in rats by subcutaneous injection of CCl4 and a high-lipid/low-protein diet for 8 wk(except the control group).The level of hyaluronic acid(HA) and alanine aminotransferase(ALT) in serum was *** activity of superoxide dismutase(SOD),glutathione-S-transferase(GST) and malondialdehyde(MDA) in liver homogenates was *** degree of hepatic fibrosis was evaluated by hematoxylin and eosin and Masson *** of Nrf2 and NADPH quinone oxidoreductase 1(Nqo1) was detected by real-time reversed transcribed-polymerase chain reaction,immunohistochemical techniques,and western ***:Compared with group B,liver indices,levels of serum HA and ALT of groups C,D and E were reduced(liver indices:0.038 ± 0.008,0.036 ± 0.007,0.036 ± 0.005 vs 0.054 ± 0.009,P<0.05;HA:502.33 ± 110.57 ng/mL,524.25 ± 255.42 ng/mL,499.25 ± 198.10 ng/mL vs 828.50 ± 237.83 ng/mL,P<0.05;ALT:149.44 ± 16.51 U/L,136.88 ± 10.07 U/L,127.38 ± 11.03 U/L vs 203.25 ± 31.62 U/L,P<0.05),and SOD level was significantly higher,but MDA level was lower,in liver homogenates(SOD:1.36 ± 0.09 U/mg,1.42 ± 0.13 U/mg,1.50 ± 0.15 U/mg vs 1.08 ± 0.19 U/mg,P<0.05;MDA:0.294 ± 0.026 nmol/mg,0.285 ± 0.025 nmol/mg,0.284 ± 0.028 nmol/mg vs 0.335 ± 0.056 nmol/mg,P<0.05).Meanwhile,the stage of hepatic fibrosis was significantly weakened(P<0.05).Compared with group A,the activity of GST liver homogenates and expression levels of Nrf2 and Nqo1 in group B were elevated(P<0.05).The expression level of Nrf2 and Nqo1 in groups C,D,and E were increased as compared with group B,but the difference was not significant

关键词： Blueberry Hepatic fibrosis NF-E2-related factor 2 NADPH quinone oxidoreductase 1 GlutathioneS-transferase

EXPERIMENTAL RESEARCH ON THE SHAPE CHARACTERS OF NATURAL AND VENTILATED SUPERCAVITATION

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Journal of Hydrodynamics 2007年第5期19卷 564-571页

作者： ZHANG Xue-wei WEI Ying-jie ZHANG Jia-zhong wang Cong yu Kai-ping School of Astronautics Harbin Institute of Technology Harbin 150001 China

A series of projectile and water-tunnel experiments were conducted to investigate the shape characters of natural and ventilated supercavitation. It was found that the shape and dimensions of both the natural supercavitation and the ventilated supercavitation are similar through the comparison of them when the cavitation number is small and equal, and the contour of both the natural supercavitation and the ventilated supercavitation can be calculated effectively with the Savchenko formula. The gravity effect can induce the asymmetry of ventilated supercavitation, and the asymmetry is more apparent as the Froude number is smaller. The empirical formula for calculating the axial deformation of ventilated supercavitation was corrected and expanded based on experiment data. The evolution rules of both the natural supercavitation and the ventilated supercavitation were described, and the vortex frequency of natural cavitation was obtained. In addition, the hysteretic aspect was observed between the development and the fall process of ventilated cavitation.

关键词： natural cavitation ventilated cavitation shapecharacters water tunnel experiment projectile experiment

Gut Microbiota-brain Axis

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Chinese Medical Journal 2016年第19期129卷 2373-2380页

作者： Hong-Xing wang yu-ping wang Department of Neurology Xuanwu Hospital Capital Medical University Beijing 100053 China Center of Epilepsy Beijing Institute for Brain Disorders Laboratory of Brain Disorders Capital Medical University Beijing 100069 China

Objective：To systematically review the updated information about the gut microbiota-brain *** Sources：All articles about gut microbiota-brain axis published up to July 18,2016,were identified through a literature search on PubMed,ScienceDirect,and Web of Science,with the keywords of＂gut microbiota＂,＂gut-brain axis＂,and ＂neuroscience＂.Study Selection：All relevant articles on gut microbiota and gut-brain axis were included and carefully reviewed,with no limitation of study ***：It is well-recognized that gut microbiota affects the brain＆#39;s physiological,behavioral,and cognitive functions although its precise mechanism has not yet been fully *** microbiota-brain axis may include gut microbiota and their metabolic products,enteric nervous system,sympathetic and parasympathetic branches within the autonomic nervous system,neural-immune system,neuroendocrine system,and central nervous ***,there may be five communication routes between gut microbiota and brain,including the gut-brain＆#39;s neural network,neuroendocrine-hypothalamic-pituitary-adrenal axis,gut immune system,some neurotransmitters and neural regulators synthesized by gut bacteria,and barrier paths including intestinal mucosal barrier and blood-brain *** microbiome is used to define the composition and functional characteristics of gut microbiota,and metagenomics is an appropriate technique to characterize gut ***：Gut microbiota-brain axis refers to a bidirectional information network between the gut microbiota and the brain,which may provide a new way to protect the brain in the near future.

关键词： Gut Microbiota Gut Microbiota-brain Axis Metagenomics Microbiome

Model and characteristic of a new underactuated manipulator and its simulation

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重庆邮电大学学报（自然科学版） 2009年第2期21卷 239-245页

作者： TAN yue-gang wang yu-ping ALL-BAIL Mohammed School of Mechanical and Electronic Engineering Wuhan University of TechnologyWuhan 430070 P. R. China

The virtual prototype of the type of under-actuated manipulator was established under the Solidworks. It is proved that the manipulator has the nonholonomic characteristic from the theoretical analysis to this model. The digital model was imported into ADAMS and the kinematics simulation and analysis were accomplished. The research conclusion shows that underactuated manipulator is controllable from the simulation analysis perspectives. It also provides theoretical foundation for prototype’s debugging and control.

关键词：运动学仿真数字模型欠驱动机械臂 Solidworks 特征 ADAMS 虚拟样机

Status of Noninvasive Brain Stimulation in the Therapy of Alzheimer's Disease

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Chinese Medical Journal 2018年第24期131卷 2899-2903页

作者： Yi-Cong Lin yu-ping wang Department of Neurology Xuanwu HospitalCapital Medical UniversityBeijing 100053China Beijing Key Laboratory of Neuromodulation Beijing 100053China Center of Epilepsy Beijing Institute for Brain DisordersCapital Medical UniversityBeijing 100069China

Alzheimer's disease (AD),characterized by progressive memory impairment and dementia leading to severe deterioration of daily living,has been a public health crisis that currently lacks effective *** reported prevalence of AD was 3.21% in people aged ≥65 years,More than 7 million people live with AD in China today and the number continues to *** imposes a heavy financial burden on the society and it is becoming *** total socioeconomic costs of Chinese patients with AD was estimated to be US $167.74 billion in 2015 and is predicted to reach US $1.89 trillion by 2050.

关键词： Alzheimer's Disease Noninvasive Brain Stimulation Repetitive Transeranial Magnetic Stimulation Transcranial Direct Current Stimulation

Effect of blueberry on hepatic and immunological functions in mice

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Hepatobiliary & Pancreatic Diseases International 2010年第2期9卷 164-168页

作者： wang, yu-ping Cheng, Ming-Liang Zhang, Bao-Fang Mu, Mao Zhou, Ming-yu Wu, Jun Li, Cheng-Xiu Guiyang Med Coll Dept Infect Dis Guiyang 550004 Peoples R China Guiyang Med Coll Dept Pharmacol Guiyang 550004 Peoples R China

BACKGROUND: Conventional drugs used in the treatment and prevention of liver diseases often have side effects, therefore research into natural substances are of significance. This study examined the effects of blueberry on liver protection and cellular immune functions. METHODS: To determine the effects of blueberry on liver protective function, male mice were orally administered blueberry (0.6 g/10 g) or normal saline for 21 days. Hepatic RNA was extracted by Trizol reagent, and the expression of Nrf2, HO-1, and Nqol was determined by real-time RTPCR. Superoxide dismutase (SOD) and malondialdehyde (MDA) in liver homogenate were determined, and liver index was measured. To assess the effects of blueberry on cellular immune function, male mice received blueberry (0.4, 0.6, or 0.8 g/10 g) for 35 days, and the percentages of CD3(+), CD41(+), and CDS(+) T lymphocyte subgroups in peripheral blood were detected by flow cytometry, the index of the thymus and spleen was measured, and lymphocyte proliferation in the spleen was determined by MTT assay. RESULTS: Blueberry treatment significantly increased the expression of Nrf2, HO-1, and Nqol, the important antioxidant components in the liver. Hepatic SOD in the blueberry group was higher and MDA was lower than that in the control group (P<0.05). Blueberry also increased the index of the spleen and enhanced the proliferation of lymphocytes of the spleen (P<0.05). The percentages of the CD3(+) and CDA(+) T lymphocyte subsets and the CD4(+)/CD8(+) ratio were also increased by blueberry (P<0.05). CONCLUSIONS: Blueberry induces expression of Nrf2, HO-1, and Nqol, which can protect hepatocytes from oxidative stress. In addition, blueberry can modulate T-cell function in mice. (Hepatobiliary Pancreat Dis Int 2010; 9: 164-168)

关键词： blueberry Nrf2 HO-1 Nqol T-cell function