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Science china(Life sciences) 2010年第3期53卷 342-347页

作者： wang yizheng & xu tianle institute of neuroscience and state key laboratory of neuroscience,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china institute of neuroscience and state key laboratory of neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

The study of ion channels represents one of the most active fields in neuroscience research in *** the last 10 years,active research in various chinese neuroscience institutions has sought to understand the mechanisms responsible for sensory processing,neural development and neurogenesis,neural plasticity,as well as *** addition,extensive studies have been directed to measure ion channel activity,structure-function relationships,as well as many other biophysical and biochemical *** review focuses on the progress achieved in the investigation of ion channels in neuronal survival during the past 10 years in china.

关键词： ion channels neuronal survival and ischemia.

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Acute modulation of ASIC3 channels by serotonin contributes to peripheral hyperalgesia

Acute modulation of ASIC3 channels by serotonin contributes ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xiang wang,Tian-Le xu~* institute of neuroscience and state key laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china

＜正＞Acid-sensing ion channels（ASICs） are proton-gated cation channels and are emerging as key sensors for extracellular protons in central and peripheral *** currents mediated by ASICs inactivate rapidly even in the continuous presence of agonist（protons）.Remarkably,type 3 ASIC（ASIC3）,which is predominantly distributed in sensory neurons,responds to mild acidosis（pH 6.8-7.2） with a sustained *** characteristics has been proposed to mediate persistent acidic pain associated with arthritis,muscular ischemia,and *** the present study,we investigated acute modulation of ASIC3-containing channels by various inflammatory mediators in both dorsal root ganglia（DRG） neurons and CHO cells expressing ASIC *** found that serotonin（5-HT）,a classical proinflammatory mediator,specifically enhanced the sustained currents of homomeric ASIC3 and heteromeric ASIC3/1b ***,5-HT directly acts on ASIC3 channels rather than through 5-HT receptors, because specific receptor antagonists did not affect 5-HT *** importantly,mutations at ASIC3（E79A, E79S,E423A and Q269A） completely abolished 5-HT-induced ***,co-application of 5-HT and acetic acid significantly increased pain-related behaviors as assayed in the paw-licking test in mice,which was largely attenuated in ASIC3-deletion mice and inhibited by the nonselective ASICs inhibitor amiloride but not by 5-HT receptor antagonists methysergide and *** together,these data identify ASIC3 channels as a molecular target for rapid actions of 5-HT and reinforce the importance of ASIC3 channels in regulating inflammatory pain.

关键词： acid-sensing ion channel 3 5-HT hyperalgesia

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The upregulation of TRPA1 following the development of diabetic peripheral neuropathy

The upregulation of TRPA1 following the development of diabe...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Sheng-Lan wang xu ZHANG~* institute of neuroscience and state key laboratory of neuroscience shanghai institutes for biological sciences chinese academy of sciences shanghai 200031 china

＜正＞Objective Diabetic neuropathy is manifested either by loss of nociception（painless syndrome） or by hyperalgesia and *** mechanism underlying neuronal injury in diabetic sensory neuropathy remains poorly understood. TRPA1,a member of the transient receptor potential（TRP） family of ion channels,is expressed by a subset of small-sized DRG or trigeminal ganglia neurons,which increased after inflammation and nerve *** try to test whether the activation of TRPA1 contributes to the diabetic peripheral *** Diabetes mellitus was induced by streptozotocin（STZ）.Diabetic hyperglycemia is defined as a nonfasting plasma glucose concentration ＞ 13 mmol/L. Mechanical and thermal behaviors were assessed by the von frey filament and heat *** expression of TRPA1 was tested by ***（1） The rat mechanical hyperalgesia was induced in 50-60%of diabetic rats at 3-4 weeks after induction of diabetic hyperglycemia,while the threshold of heat was increased in 70-80%of diabetic rats at 6-8 weeks after induction of diabetic hyperglycemia.（2） The expression of TRPA1 mRNA in DRG neuron was increased after heat insensitivity induced in diabetic *** We hypothesized that hyperglycemia-induced endogenous products acting on the TRPA1 ion channel contributes to development and maintenance of diabetic peripheral neuropathy.

关键词： diabetic peripheral neuropathy TRPAl mechanical hyperalgesia heat insensitivity

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FXYD domain-containing ion transport regulator 2 in the small-diameter neurons of dorsal root ganglion regulates nociception

FXYD domain-containing ion transport regulator 2 in the smal...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Feng wang,xu ZHANG~* institute of neuroscience and state key laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai,china

Objective FXYD domain-containing ion transport regulator 2(FXYD2) is a member of FXYD protein family which shares a signature sequence of six conserved amino acids comprising the FXYD motif in the N-terminus,and two glycine and one serine residues in the transmembrane ***2 was originally characterized as a small acidic proteolipid that co-purified with renal Na,K-ATPase and which,similar to the a subunit of Na,K-ATPase,covalently bound to photoaffinity derivatives of *** FXYD2 was cloned,FXYD2 was confirmed to be indeed associated specifically with Na,*** measurement of Na,K-ATPase transport in Xenopus oocytes revealed that FXYD2 decreases the apparent affinity of the Na,K-ATPase for intracellular *** the cortical collecting duct of kidney,which lacks FXYD2,the constant for half-maximal activation of Na,K-ATPase by Na is lower than that in the thick ascending limb(TAL),where FXYD2 is abundant,consistent with the result from ***2 was expressed mostly in the kidney in ***,in the present study we found that FXYD2 was also highly expressed in the dorsal root ganglion(DRG).The expression pattern of FXYD2 in the DRG and the regulation of nociception by FXYD2 were further *** Microarray and RT-PCR were carried out to study the tissue distribution of ***,we used in situ hybridization to investigate the expression profile of FXYD2 in rat *** decrease of expression in the axotomy model was also confirmed by in situ ***2 knockout mice were used to study the regulation of pain by FXYD2,with the behavior test of tailflick,hot plate,von Frey,and ***(1) FX YD2 was highly expressed in DRG compared to other tissues,besides *** rat DRG,FXYD2 was exclusively expressed in the small-diameter neurons.(2) The expression of FXYD2 was down-regulated in the ipsilateral DRG in the neuropathic model. (3) The locomotor function of FXYD2 knockout mice was normal as checked

关键词： FXYD domain-containing ion transport regulator 2/FXYD2 nociception Na+,K+-ATPase

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Membrane insertion of plasmalemmal precursor vesicles（PPV） is mediated by RablO interaction with MARCKS during axonal growth

Membrane insertion of plasmalemmal precursor vesicles（PPV）...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Cai-Yun DENG~(**),Xiao-Hui xu~(**),Yang LIU,Tong wang,Zhen-Ge LUO~* institute of neuroscience and state key laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china

＜正＞The formation of axons and dendrites and maintenance of the neuron’s vastly expanded surface require the continuous addition of new *** axon extension,the coordination of membrane traffic,adhesion to extracellular substrate,and reorganization of the cytoskeleton at the peripheral region of the growth cone mediate the protrusive activity of the axon growth *** we have found the involvement of Rab10 in directional membrane insertion of plasmalemmal precursor vesicles（PPV） during neuronal *** the mechanism of Rab10 action remains *** we report that the myristoylated alanine-rich C kinase substrate（MARCKS） interacts with Rab10 and mediates fusion of PPV to the plasma ***-regulation of MARCKs using siRNA against MARCKS abrogated polarized distribution of Rab10 and prevented neuronal ***, blockade of the interaction between Rab10 and MARCKS by over-expressing the MARCKS domain that mediated the RablO-MARCKS binding abolished membrane fusion of PPVs,which were labeled with BODIPY-ceramide. Membrane fusion of PPVs was also measured by analyzing the TIRF（Internal Reflection Fluorescence） signals of pHluorin-tagged receptors of axon growth promoting factors - TrkB for brain-derived neurotrophic factor（BDNF） and IGFR for insulin like factor 1（IGF-1）.We found that down-regulation of MARCKS or blocking its interaction with Rab10 inhibited the TIRF ***,blocking the interaction between Rab10 and MARCKS inhibited axon growth in vitro and in ***,Rab10 interaction with MARCKS mediates fusion of PPV with the plasma membrane,a process required for axonal growth.

关键词： MARCKS Rab10 plasmalemmal precursor vesicle axonal growth fusion membrane insertion

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Membrane insertion of plasmalemmal precursor vesicles（PPV） is mediated by RablO interaction with MARCKS during axonal growth

Membrane insertion of plasmalemmal precursor vesicles（PPV）...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xiao-Hui xu~#,Cai-Yun DENG~#,Yang LIU,Tong wang,Zhen-Ge LUO~* institute of neuroscience and state key laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china

＜正＞The formation of axons and dendrites and maintenance of the neuron’s vastly expanded surface require the continuous addition of new *** axon extension,the coordination of membrane traffic,adhesion to extracellular substrate,and reorganization of the cytoskeleton at the peripheral region of the growth cone mediate the protrusive activity of the axon growth *** we have found the involvement of Rab10 in directional membrane insertion of plasmalemmal precursor vesicles（PPV） during neuronal *** the mechanism of Rab10 action remains *** we report that the myristoylated alanine-rich C kinase substrate（MARCKS） interacts with Rab10 and mediates fusion of PPV to the plasma ***-regulation of MARCKs using siRNA against MARCKS abrogated polarized distribution of Rab10 and prevented neuronal ***, blockade of the interaction between Rab10 and MARCKS by over-expressing the MARCKS domain that mediated the Rab10-MARCKS binding abolished membrane fusion of PPVs,which were labeled with BODIPY-ceramide. Membrane fusion of PPVs was also measured by analyzing the TIRF（Internal Reflection Fluorescence） signals of pHluorin-tagged receptors of axon growth promoting factors - TrkB for brain-derived neurotrophic factor（BDNF） and IGFR for insulin like factor 1（IGF-1）.We found that down-regulation of MARCKS or blocking its interaction with Rab10 inhibited the TIRF ***,blocking the interaction between Rab10 and MARCKS inhibited axon growth in vitro and in ***,Rab10 interaction with MARCKS mediates fusion of PPV with the plasma membrane,a process required for axonal growth.

关键词： MARCKS RablO plasmalemmal precursor vesicle axon growth docking fusion

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Fibroblast growth factor 7 acts as a modulator of nociception

Fibroblast growth factor 7 acts as a modulator of nociceptio...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hui LIU~1,Qing-Feng WU~1,Qiong wang~2,Lan BAO~2,xu ZHANG~1 1 institute of neuroscience and state key laboratory of neuroscience,2 institute of Biochemistry and Cell Biology and laboratory of Molecular Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences, shanghai 200031,china

＜正＞Fibroblast growth factor（FGF）7,which is also known as keratinocyte growth factor（KGF）,is a member of the FGF *** studies regard it as a locally acting epithelial mitogen that is produced by cells of mesenchymal origin and has an important role in protecting and repairing epithelial ***,its potential functions in the nervous system are poorly *** found that FGF7,together with its high-affinity receptor FGFR2b,was expressed in both dorsal root ganglion（DRG）and spinal cord ***7 mRNA was consistently increased in DRGs after peripheral nerve injury and transiently up-regulated after intraplantar injection of complete Freund’s *** injection of recombinant human FGF7 exacerbated the formalin-induced hyperalgesia, while application with either the antibodies against FGF7 or recombinant FGF R2β（Ⅲb）Fc chimera alleviated inflammatory nociceptive ***,behavior tests and wide-dynamic range neuron recordings of FGF7 knockout mice showed a defect in nociceptive *** together,these results suggest that FGF7 may contribute to inflammatory pain sensation and play an important role in nociceptive transmission.

关键词： fibroblast growth factor 7 inflammatory pain nociceptive transmission

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Anticonvulsant activity of BmK AS,a sodium channel site 4-specific modulator in rats

Anticonvulsant activity of BmK AS,a sodium channel site 4-sp...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Chun-Chun WENG~1 Rong ZHAO~2 Qi FENG~1 xu-Ying ZHANG~2 Yun wang~3 Yong-Hua JI~(1 *) 1 The laboratory of Neuropharmacology and Neurotoxicology shanghai University shanghai 200444 china 2 Graduate School of the chinese academy of sciences institute of Physiology shanghai institutes for biological sciences chinese academy of sciences shanghai 200031 china 3 institutes of Brain Science and state key laboratory of Medical Neurobiology Fudan University shanghai 200032 china

＜正＞Objective To investigate the anticonvulsant activity of BmK AS,a sodium channel site 4-specific modulator purified from scorpion Buthus martensi Karsch（BmK） in rat models of acute seizures induced by pentylenetetrazole（PTZ） or *** In the present study,freely moving rats were randomized to receive either saline or BmK AS （0.05-1μg in 1μl） by microinjection into the CAl area.30min after BmK AS pre-treatment,these rats were made epileptic by administration of either PTZ or *** assessments of BmK AS were based on PTZ-induced seizurelike behaviour and cortex epileptiform EEG,as well as pilocarpine-induced seizure-like behaviour and c-Fos expression. Results Intrahippocampal CA1 injection of BmK AS has shown a dose-dependent anticonvulsant effect on both models of chemically-induced *** PTZ model,BmK AS suppressed seizure behavior and also inhibited epileptiform EEG component in both numbers and duration of high amplitude and frequency discharges（HAFDs）.In pilocarpine model, BmK AS also increased the latency to status epilepticus（SE） onset,reduced seizure score and suppressed the expression of *** The results demonstrated that the sodium channel modulator BmK AS is an effective inhibitor for acute seizure,which may be used as a novel tool to explore the molecular and pathological mechanism of epilepsy related to sodium channels.

关键词： BmK AS intrahippocampal injection status epilepticus sodium channels

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Lipid rafts mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X3 receptor

Lipid rafts mediates ATP-induced endocytosis,signal transduc...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： xu-Qiao CHEN~(1,#),Bin wang~(1,#),Chengbiao WU~2,Jin PAN~1,Bo YUAN~3,Yuan-Yuan SU~1,Xing-Yu JIANG~3,xu ZHANG~4 Lan BAO~(1,*) 1 laboratory of Molecular Cell Biology,institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 2 Department of neurosciences,School of Medicine,University of California,San Diego,La Jolla,California,USA 3National Center of Nanoscience and Technology,Beijing 100190,china 4 institute of neuroscience and state key laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china

P2X receptors are a family of cation channels expressed in neurons and nonneuronal *** play a variety of roles including synaptic transmission,muscle contraction,neuronal secretion and inflammation response. Among P2X receptors,the P2X subtype is selectively expressed in dorsal root ganglion *** raft is a specialized membrane microdomain and participate many signal transduction *** report showed that P2X receptor is localized in the lipid rafts of DRG *** have found P2X receptor engaged in retrograde transport in the form of signaling *** we investigated the role of lipid rafts in a,B-MeATP-dependent endocytosis and signal transduction,and retrograde transport of P2X *** of lipid raft with MBCD or nystatin abolished ATP-dependent endocytosis of P2X receptor and ATP-induced ERK activation in P2X receptor-expressing HEK293 *** vivo sucrose gradient fractionation with vesicular pellets prepared from sciatic nerve axoplasm showed that abundant P2X receptor was localized in lipid raft *** transported P2X receptors were colocalized with the lipid raft marker caveolinla in sciatic *** terminal treatment with MPCD attenuated retrograde transport of P2X receptor in axon of dorsal root ganglion ***,lipid raft mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X receptor.

关键词： P2X3 receptor lipid raft endocytosis ERK retrograde transport

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ATP-dependent retrograde axonal transport of P2X3 receptor in primary sensory neurons

ATP-dependent retrograde axonal transport of P2X3 receptor i...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Bin wang~1,xu-Qiao CHEN~1,Cheng-Biao WU~2,Jin PAN~1,Bo YUAN~3,Yuan-Yuan SU~1,Xing-Yu JIANG~3, xu ZHANG~4,Lan BAO~(1,*) 1 laboratory of Molecular Cell Biology,institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 2 Department of neurosciences,School of Medicine,University of California,San Diego,La Jolla,California 92093- 0662,USA 3 National Center for Nanoscience and Technology,Beijing 100080,china 4 institute of neuroscience and state key laboratory of neuroscience,institutes for biological sciences,chinese academy of sciences,shanghai 200031,china

Objective P2X receptors are ATP-gated cation channels and play variable roles in pathophysiological *** P2X receptors,P2X

关键词： primary sensory neuron P2X3 receptor axonal retrograde transport

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