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Gene characterization and phylogenetic analysis of four mitochondrial genomes in Caenogastropoda

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Acta Oceanologica Sinica 2024年第2期43卷 137-150页

作者： Jiangyong Qu Wanqi Yang Xindong Teng Li Xu Dachuan Zhang Zhikai Xing Shuang wang Xiumei Liu Lijun wang xumin wang College of Life Science Yantai UniversityYantai 264005China Qingdao International Travel Healthcare Center Qingdao 266071China Qingdao Dagang Customs Qingdao 266071China

Caenogastropoda is a highly diverse group,containing~60%of all existing *** in this subclass predominantly inhabit marine environments and have a high ecological and economic *** to the increase in relevant phylogenetic studies,our understanding of between species relatedness in Caenogastropoda has ***,the biodiversity,taxonomic status,and phylogenetic relationships of this group remain *** the present study,we performed next-generation sequencing of four complete mitochondrial genomes from three families(Buccinidae,Columbellidae,and Cypraeidae)and the four mitogenomes were classical circular structures,with a length of 16177 bp in Volutharpa ampullacea,16244 bp in Mitrella albuginosa,16926bp in Mauritia arabica asiatica and 15422 bp in Erronea *** composition analysis indicated that whole sequences were biased toward A and *** compared them with 171 complete mitochondrial genomes of *** phylogenetic relationship of Caenogastropoda derived from Maximum Likelihood(ML)and Bayesian Inference(BI)trees constructed based on CDS sequences was consistent with the results of traditional morphological analysis,with all three families showing close *** study supported Caenogastropoda at the molecular level as a separate clade of *** to our divergence time estimations,Caenogastropoda was formed during the middle Triassic period(~247.2–237 Ma).Our novel mitochondrial genomes provide evidence for the speciation of Caenogastropoda in addition to elucidating the mitochondrial genomic evolution of this subclass.

关键词： mitochondrial genome phylogenetic analysis Caenogastropoda

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天水市花椒山楂叶螨发生与药剂防治试验

林业科技通讯 2024年第6期 103-105页

作者：王旭民王喜梅天水市植物保护植物检疫站甘肃天水741020

阐述了天水花椒山楂叶螨(Tetranychus viennensis)的危害现状和发生特点,并开展了田间药效试验。结果表明:山楂叶螨的发生与温湿度密切相关,高温干旱有利于叶螨的发生;药剂防治中,20.8%阿维·四螨嗪悬浮剂、24.5%阿维·矿物油乳油、3%... 详细信息

阐述了天水花椒山楂叶螨(Tetranychus viennensis)的危害现状和发生特点,并开展了田间药效试验。结果表明:山楂叶螨的发生与温湿度密切相关,高温干旱有利于叶螨的发生;药剂防治中,20.8%阿维·四螨嗪悬浮剂、24.5%阿维·矿物油乳油、3%阿维菌素微乳剂、73%炔螨特乳油等4种药剂药后1 d的防效分别是90.83%、85.57%、87.53%、89.41%,药后7 d的防效分别是96.09%、91.11%、93.78%、94.89%,药后14 d的防效分别是93.56%、93.39%、92.22%、91.88%,上述药剂防治花椒山楂叶螨效果良好,可在生产中轮换使用。

关键词：山楂叶螨 Tetranychus viennensis 特点杀虫剂田间防效

黄河三角洲湿地生态修复区大型底栖动物群落结构与生态健康评价

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生物多样性 2024年第3期32卷 74-86页

作者：倪艳梅陈莉董志远孙德斌李宝泉王绪敏陈琳琳烟台大学生命科学学院山东烟台264003 中国科学院烟台海岸带研究所海岸带生物学与生物资源保护实验室山东烟台264003 中国科学院海岸带环境过程与生态修复重点实验室(中国科学院烟台海岸带研究所) 山东烟台264003

黄河三角洲国际重要湿地生物多样性保护工程于2019年动工,本研究基于修复区内大型底栖动物群落组成特点及其与环境因子的相关关系,分析大型底栖动物群落恢复特征,了解该地区的生态系统健康状况,评估保护工程实施三年后修复区湿地生态系... 详细信息

黄河三角洲国际重要湿地生物多样性保护工程于2019年动工,本研究基于修复区内大型底栖动物群落组成特点及其与环境因子的相关关系,分析大型底栖动物群落恢复特征,了解该地区的生态系统健康状况,评估保护工程实施三年后修复区湿地生态系统演替阶段及修复效果。结果表明:2022年春、夏、秋三季共采集到大型底栖动物16种,隶属于5门6纲15科,昆虫纲动物在物种组成中占据优势地位;大型底栖动物密度和生物多样性指数的时空差异均不显著(P>0.05);大型底栖动物生物量存在显著的季节差异,表现为秋季(2.89 g/m^(2))>夏季(1.95 g/m^(2))>春季(1.90 g/m^(2)),秋季与春季和夏季间均存在显著差异(P0.05)。与环境因子的相关性分析结果显示,蠓科一种(Ceratopogonidae sp.)、石缨虫属一种(Laonome sp.)、椭圆萝卜螺(Radix swinhoei)、尖口圆扁螺(Hippeutis cantori)等物种密度与电导率、盐度、总碳、铵盐和亚硝酸盐等指标显著相关(P<0.01)。快速生物综合评价指数评定修复区为“亚健康”状态。本研究通过对黄河三角洲湿地修复区大型底栖动物群落特征以及生态健康评价的分析,为修复区底栖动物群落的演替规律研究提供基础数据和科学依据,为后续湿地修复工程的实施和管理提供指导和参考,对于黄河三角洲生物多样性的保护具有重要意义。

关键词：黄河三角洲生态修复大型底栖动物快速生物评价群落结构

Comparison of nonlinear modeling methods for the composite rubber clamp

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Applied Mathematics and Mechanics(English Edition) 2024年第5期45卷 763-778页

作者： Yiming CAO Hui MA xumin GUO Bingfeng ZHAO Hui LI Xin wang Bing wang School of Mechanical Engineering and Automation Northeastern UniversityShenyang 110819China Key Laboratory of Vibration and Control of Aero-Propulsion Systems of Ministry of Education of China Northeastern UniversityShenyang 110819China AVIC Shenyang Engine Design Institute Shenyang 110013China Dalian Mengyu Machinery Co. Ltd.Dalian 116317Liaoning ProvinceChina

The cubic stiffness force model(CSFM)and Bouc-Wen model(BWM)are introduced and compared *** unknown coefficients of the nonlinear models are identified by the genetic algorithm combined with *** fitting the identified nonlinear coefficients under different excitation amplitudes,the nonlinear vibration responses of the system are *** results show that the accuracy of the BWM is higher than that of the CSFM,especially in the non-resonant ***,the optimization time of the BWM is longer than that of the CSFM.

关键词： pipeline system nonlinear clamp model composite rubber clamp amplitude-dependent characteristic vibration response experiment

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柳树杂种无性系苗期生长与枝条观赏特性研究

林业科技 2024年第2期49卷 1-6页

作者：崔胜佳王旭敏郭欢欢刘兴菊梁海永河北农业大学林学院河北保定071000 河北省种质资源与森林保护重点实验室河北保定071000

为了研究白柳×旱柳的杂交后代在枝条和叶片上的观赏性特点以及其呈色原理,本研究以枝条颜色差异明显的白柳和旱柳的杂交后代作为研究对象,分为R组(红枝)(R1、R2、R3)和Y组(黄枝)(Y1、Y2、Y3),‘普通旱柳’(HL,绿色)作为对照,对其枝条... 详细信息

为了研究白柳×旱柳的杂交后代在枝条和叶片上的观赏性特点以及其呈色原理,本研究以枝条颜色差异明显的白柳和旱柳的杂交后代作为研究对象,分为R组(红枝)(R1、R2、R3)和Y组(黄枝)(Y1、Y2、Y3),‘普通旱柳’(HL,绿色)作为对照,对其枝条颜色、形态、花青素含量以及叶片形态、颜色、叶绿素含量等指标进行测定和分析。结果表明:R组的枝条花青素的含量高于Y组和HL,具有极显著差异。R组、Y组的叶片叶绿素含量均与HL存在显著差异,叶绿素与类胡萝卜素的比值在R组和HL之间存在显著差异,R组的比值更高,叶绿素占比高。Y组的比值偏小,类胡萝卜素所占的比例较高。这些因素综合作用使得R组叶片和枝条颜色的a值均较高,叶片颜色更绿,枝条颜色呈现红色;Y组叶片和枝条的颜色b值均高于对照组,叶片颜色呈现黄绿色,枝条颜色为黄色。R组和Y组叶片的宽长比均和HL存在着显著差异,叶片形态大致区分阔披针形、窄披针形和披针形。综合分析得出:杂交后代的表现型与普通品种之间存在显著的差别,存在更丰富的遗传多样性,具有高于普通旱柳更多的观赏特性。

关键词：杂种柳树花青素观赏特性

宁夏畜禽粪污肥料化利用现状、存在问题及发展思路

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现代畜牧科技 2024年第5期 113-117页

作者：王明国耿荣崔勇王旭敏丁永峰李志广王晓媛张战胜宁夏农业技术推广总站宁夏银川750001 宁夏青铜峡市农业技术和农机化推广服务中心宁夏吴忠751600 宁夏固原市泾源县农业技术推广服务中心宁夏固原756400

畜禽粪污处理问题是制约宁夏乃至全国畜禽养殖业发展的瓶颈。该文在深入调查宁夏畜牧业发展及畜禽粪污肥料化利用情况的基础上,阐述了畜禽粪污资源及养殖业粪污处理现状,分析了养殖业粪污处理存在的困难与问题,以期为今后开展畜禽粪污... 详细信息

畜禽粪污处理问题是制约宁夏乃至全国畜禽养殖业发展的瓶颈。该文在深入调查宁夏畜牧业发展及畜禽粪污肥料化利用情况的基础上,阐述了畜禽粪污资源及养殖业粪污处理现状,分析了养殖业粪污处理存在的困难与问题,以期为今后开展畜禽粪污处理工作提供参考。

关键词：畜禽粪污肥料利用

基于16S rRNA测序的1型和2型糖尿病大鼠肠道菌群差异

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烟台大学学报（自然科学与工程版） 2024年第2期37卷 163-171页

作者：倪妍滕新栋徐丽王绪敏曲江勇王丽君金黎明邢志凯刘秀梅烟台大学生命科学学院山东烟台264005 青岛国际旅行卫生保健中心山东青岛266071 青岛大港海关山东青岛266071 大连民族大学生物技术与生物资源利用重点实验室辽宁大连116600

通过16S rRNA测序分析T1DM与T2DM大鼠肠道菌群的差异。Alpha多样性分析显示,T1DM组和T2DM组肠道菌群多样性和均匀度均高于对照组,Beta多样性分析显示不同组样本间存在差异且有很好的聚类。在门水平上,T1DM和T2DM大鼠肠道菌群以Firmicute... 详细信息

通过16S rRNA测序分析T1DM与T2DM大鼠肠道菌群的差异。Alpha多样性分析显示,T1DM组和T2DM组肠道菌群多样性和均匀度均高于对照组,Beta多样性分析显示不同组样本间存在差异且有很好的聚类。在门水平上,T1DM和T2DM大鼠肠道菌群以Firmicutes和Bacteroidetes为主,Firmicutes/Bacteroidetes(F/B)比例相比于对照组增加,且T2DM组高于T1DM组。与对照组相比,T1DM组Actinobacteria丰度显著增加,Anaerofustis丰度显著降低。在T2DM大鼠中,Proteobacteria、Oscillospira丰度显著增加。Oscillospira丰度在T2DM组增加,在T1DM组无明显差异。Rhodococcus为T1DM组的特有属,Coprobacillus和Roseburia为T2DM组的特有属。研究结果表明大鼠肠道菌群失调与T1DM和T2DM密切相关,可为T1DM和T2DM患者的个性化治疗提供科学依据。

关键词：肠道菌群 T1DM T2DM 16S rRNA测序

Phylogenetic analyses of the genes involved in carotenoid biosynthesis in algae

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Acta Oceanologica Sinica 2018年第4期37卷 89-101页

作者： wang Shanshan ZHANG Lei CHI Shan wang Guoliang wang xumin LIU Tao TANG Xuexi College of Marine Life Sciences Ocean University of China CAS Key Laboratory of Genome Sciences and Information/Beijing Key Laboratory of Genome and Precision Medicine Technologies Beijing Institute of GenomicsChinese Academy of Sciences

Carotenoids play a crucial role in absorbing light energy for photosynthesis,as well as in protecting chlorophyll from *** contrast to the Streptophyta,few studies have examined carotenoid biosynthetic pathways in algae,owing to a shortage of *** part of the 1000 Plants Project,we sequenced and assembled the transcriptomes of 41 marine macroalgal species,including 22 rhodophytes and 19 phaeophytes,and then combined the datasets with publicly available data from Gen Bank（National Center for Biotechnology Information） and the *** of Energy Joint Genome *** a result,we identified 68 and 79 fulllength homologs in the Rhodophyta and Phaeophyceae,respectively,of seven inferred carotenoid biosynthetic genes,including the genes for phytoene synthase（PSY）,phytoene desaturase（PDS）,ζ-carotene desaturase（ZDS）,ζ-carotene isomerase（Z-ISO）,prolycopene isomerase（crt ISO）,lycopene β-cyclase（LCYB）,and lycopene ε-cyclase（LCYE）.We found that the evolutionary history of the algal carotenoid biosynthetic pathway was more complex than that of the same pathway in the Streptophyta and,more specifically,that the evolutionary history involved endosymbiotic gene transfer,gene duplication,and gene *** all of the eukaryotic algae that we examined had inherited the seven carotenoid biosynthesis genes via endosymbiotic gene ***,PSY,crt ISO,and the ancestral lycopene cyclase gene（LCY） underwent duplication events that resulted in multiple gene copies,and the duplication and subsequent divergence of LCYB and LCYE specialized and complicated the cyclization of *** findings also verify that the loss of LCYE in both the microphytic rhodophytes and phaeophytes explains the differences in their carotenoid patterns,when compared to the macrophytic *** analyses provide a molecular basis for further biochemical and physiological validation in additional algal species and should help elucidate the origin and evolution of caro

关键词： carotenoid biosynthesis algae phylogenetic analysis

Origin and evolution of alginate-c5-mannuronan-epimerase gene based on transcriptomic analysis of brown algae

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Acta Oceanologica Sinica 2014年第2期33卷 73-85页

作者： wang Ren wang xumin ZHANG Yalan YU Jun LIU Tao CHEN Shengping CHI Shan College of Marine Life Science Ocean University of China CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome andPrecision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChineseAcademy of Sciences Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism Zhong ShanSchool of MedicineSun Yat-Sen University

The coding product of alginate-c5-mannuronan-epimerase gene （algG gene） can catalyze the conversion of mannuronate to guluronate and determine the M/G ratio of alginate. Most of the current knowledge about genes involved in the alginate biosynthesis comes from bacterial systems. In this article, based on some algal and bacterial algG genes registered on GenBank and EMBL databases, we predicted 94 algG genes open reading frame （ORF） sequences of brown algae from the 1 000 Plant Transcriptome Sequencing Project （OneKP）. By method of transcriptomic sequence analysis, gene structure and gene localization analysis, multiple sequence alignment and phylogenetic tree construction, we studied the algal algG gene family characteristics, the structure modeling and conserved motifs of AlgG protein, the origin of alginate biosyn-thesis and the variation incidents that might have happened during evolution in algae. Although there are different members in the algal algG gene family, almost all of them harbor the conserved epimerase region. Based on the phylogenetic analysis of algG genes, we proposed that brown algae acquired the alginate bio-synthesis pathway from an ancient bacterium by horizontal gene transfer （HGT）. Afterwards, followed by duplications, chromosome disorder, mutation or recombination during evolution, brown algal algG genes were divided into different types.

关键词： transcriptomic sequencing alginate-c5-mannuronan-epimerase gene gene family alginate phylogenetic analysis