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Oral attenuated Salmonella typhimurium vaccine against MG7-Ag mimotope of gastric cancer

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World Journal of Gastroenterology 2005年第12期11卷 1833-1836页

作者： Fan-PingMeng JieDing Zhao-CaiYu Quan-lihan Chang-CunGuo NaLiu Dai-MingFan InstituteofDigestiveDisease XijingHospitalFourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina

AIM: To develop an oral attenuated Salmonella typhimurium vaccine against gastric cancer and to evaluate its efficacy in mice. METHODS: A complementary sequence of Nco Ⅰ site and a sequence coding for MG7-Ag mimotope were designed at the 5'terminus of forward primer. Using p1.2 Ⅱ-HBCAg plasmid as template, PCR was performed to get a fusion gene of the mimotope and a HBcAg gene. The fusion gene was then subcloned into the plasmid pYA3341 complementary to Salmonella typhimurium X4550, and the recombinant plasmid was then transformed into attenuated Salmonella typhimurium X4550. Balb/c mice were orally immunized with the recombinant Salmonella typhimurium X4550. The mice were immunized every 2 wk to reinforce the immunity. At the 6th wk, serum titer of antibody was detected by ELISA, and at the 8th wk, cellular immunity was detected by 51Cr release test. Ehrlich ascites carcinoma cells expressing MG7-Ag were used in tumor challenge assay as a model to evaluate the protective effect of the vaccine. RESULTS: Serum titer of antibody against MG7-Ag was significantly higher in mice immunized with the vaccine than in control groups (0.9538±0.043 vs 0.6531±0.018, P<0.01; 0.9538±0.043 vs 0.6915±0.012, P<0.01), while in vitro 51Cr release assay of the splenocytes showed no statistical difference in the three groups. Two weeks after tumor challenge, 1 in 5 immunized mice was tumor free, while all the mice in the control group presented tumor. CONCLUSION: Oral attenuated Salmonella typhimurium vaccine against the MG7-Ag mimotope of gastric cancer is immunogenic. It can induce significant humoral immunity against tumors in mice, and has some protective effects.

关键词： Salmonella typhimuriunr Gastric cancer

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飞轮锂电池混合储能系统的VSG控制策略

黑龙江科技大学学报 2024年第1期34卷 132-137页

作者：薛易李天意吴立涵龙腾飞黑龙江科技大学电气与控制工程学院哈尔滨150022

为提升电网电能质量,抑制电网在运行过程的频率和电压超波动,采用飞轮锂电池混合储能系统进行波动平抑,考虑飞轮作用时间短,蓄电池作用时间长,在VSG控制中引入一阶高通滤波器,通过蓄电池参与一次调频,飞轮参与调压。结果表明,该策略能... 详细信息

为提升电网电能质量,抑制电网在运行过程的频率和电压超波动,采用飞轮锂电池混合储能系统进行波动平抑,考虑飞轮作用时间短,蓄电池作用时间长,在VSG控制中引入一阶高通滤波器,通过蓄电池参与一次调频,飞轮参与调压。结果表明,该策略能够实现电压维持在690 V,频率维持在50 Hz,实现了对频率和电压良好平抑,具有很好的鲁棒性。

关键词：混合储能系统虚拟同步发电机波动平抑

Long-tailed object detection of kitchen waste with class-instance balanced detector

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Science China(Technological Sciences) 2023年第8期66卷 2361-2372页

作者： FANG LeYuan TANG Qi OUYANG lihan YU JunWu LIN JiaXing DING ShuaiYu TANG Lin College of Electrical and Information Engineering Hunan UniversityChangsha 410082China College of Environmental Science and Engineering Hunan UniversityChangsha 410082China

Intelligent detection and classification of kitchen waste can promote ecological sustainability by replacing inefficient manual ***,the presence of non-degradable waste mixed in kitchen waste often follows a long-tailed distribution,making it challenging to train convolutional neural network-based object detectors,which results in the unsatisfactory detection of tailclass *** address this challenge,we propose a class-instance balanced detector(CIB-Det) for intelligent detection and classification of kitchen ***-Det implements two strategies for the loss function:the class-balanced strategy(CBS)and the instance-balanced strategy(IBS).The CBS focuses more on tail classes,and the IBS concentrates on hard-to-classify instances adaptively during ***,CIB-Det comprehensively and adaptively addresses the long-tailed *** experiments on a real dataset of kitchen waste images support the effectiveness of CIB-Det for kitchen waste detection.

关键词： kitchen waste detection and classification object detection long-tailed distribution convolutional neural networks

A combination of hierarchical pore and buffering layer construction for ultrastable nanocluster Si/SiO_(x)anode

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Nano Research 2020年第11期13卷 2987-2993页

作者： Kun Zeng Tong Li Xianying Qin Gemeng Liang lihan Zhang Qi Liu Baohua Li Feiyu Kang Shenzhen Key Laboratory of Power Battery Safety Research and Shenzhen Geim Graphene Center Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen518055China Tsinghua-Berkeley Shenzhen Institute(TBSI) Tsinghua UniversityShenzhen518055China School of Materials Science and Engineering Tsinghua UniversityBeijing100084China

Porous Si can be synthesized from diverse silica(SiO_(2))via magnesiothermic reduction technology and widely employed as potential anode material in lithium ion ***,concerns regarding the influence of residual silicon oxide(SiO_(x))component on resulted Si anode after reduction are still *** this work,we intentionally fabricate a cauliflower-like silicon/silicon oxide(CF-Si/SiO_(x))particles from highly porous SiO_(2)spheres through insufficient magnesiothermic reduction,where residual SiO_(x)component and internal space play an important role in preventing the structural deformation of secondary bulk and restraining the expansion of Si ***,the hierarchically structured CF-Si/SiO_(x)exhibits uniformly-dispersed channels,which can improve ion transport and accommodate large volume expansion,*** a result,the CF-Si/SiO_(x)-700 anode shows excellent electrochemical performance with a specific capacity of^1,400 mA·h·g^(−1)and a capacity retention of 98%after 100 cycles at the current of 0.2 A·g^(−1).

关键词： highly-porous Si/SiO_(x)anodes magnesiothermic reduction lithium ion battery

直驱风机并入交流电网引发的次同步振荡分析与抑制

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黑龙江电力 2024年第2期46卷 146-150页

作者：薛易吴立涵龙腾飞李天意黑龙江科技大学电气与控制工程学院哈尔滨150022

通过对直驱风机并网模型及短路比原理分析,阐述次同步振荡的发生机理。提出在高压母线侧加装静止无功补偿器去抑制次同步振荡的发生,并对静止无功补偿器的控制策略进行分析。结果表明,该控制策略能有效抑制次同步振荡现象,保障电力系统... 详细信息

通过对直驱风机并网模型及短路比原理分析,阐述次同步振荡的发生机理。提出在高压母线侧加装静止无功补偿器去抑制次同步振荡的发生,并对静止无功补偿器的控制策略进行分析。结果表明,该控制策略能有效抑制次同步振荡现象,保障电力系统的安全与稳定。

关键词：直驱风机次同步振荡静止无功补偿器短路比

Effect of environmental stresses on the survival and cytotoxicity of Shiga toxin-producing Escherichia coli

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Food Quality and Safety 2017年第2期1卷 139-146页

作者： Brian Byongkwon Yoo Yanhong Liu Vijay Juneja lihan Huang Cheng-An Hwang Residue Chemistry and Predictive Microbiology Research Unit and Molecular Characterization of Foodborne Pathogens Research Unit Eastern Regional Research CenterAgricultural Research ServiceUnited States Department of AgricultureWyndmoorPennsylvaniaUSA Present address:Clinical and Environmental Microbiology Branch Division of Healthcare Quality and PromotionNational Center for Emerging and Zoonotic Infectious DiseasesCenters for Disease Control and Prevention1600 Clifton Rd.NEAtlantaGA 30329USA

The objective of this study was to determine the effect of environmental stresses on the cytotoxicity of Shiga toxin-producing Escherichia coli (STEC). STEC O157:H7 and six non-O157 STEC strains (O26:H11, O103:H1, O104:H4, O111:NM, O121:NM, and O145:NM) were subjected to osmotic (aw 0.95-0.98), acid (pH 4-7), and chlorine (1-5 ppm) stresses. After stress treatments, bacterial populations, expression of virulence-associated genes, and Vero-cytotoxicity were determined. Among the strains, O145:NM survived at aw 0.97 longer than other serotypes, while O111:NM was significantly more sensitive to osmotic stress. At pH 4, O103:H1 was more resistant to the stress, while O26:H11 and O111:NM had significantly less growth. For 2 ppm chlorine stress, O26:H11, O103:H1, and O145:NM had higher populations (>3 log) than other strains. Stressed strains showed a significant increase in relative gene expression levels of stx1, stx2, and eae in O103:H1, O104:H4, and O145:NM than non-stressed control. Additionally, significantly higher Vero-cytotoxicity, as indicated by lactate dehydrogenase assay, of stressed O26:H11, O103:H1, O104:H4, and O145:NM was observed. The results suggest that the growth and cytotoxicity of selected pathogenic E. coli may be enhanced after being exposed to environmental stresses.

关键词： Pathogenic Escherichia coli stress growth virulence genes Vero-cytotoxicity.

冲突归因与调节焦点对工程冲突解决行为的影响研究

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工程管理学报 2024年第4期38卷 113-117页

作者：张丽晗娄建民郭文倩徐振洋河北工业大学经济管理学院天津300130 北京交通大学经济管理学院北京100044 天津大学管理与经济学部天津300072

冲突在工程项目中难以避免,从心理角度分析冲突归因及冲突方的调节焦点如何影响其冲突解决行为。应用多元层次回归分析法分析了368份由工程项目从业人员填写的有效问卷,以检验提出的研究假设。结果表明,对冲突的内在归因促进冲突方采取... 详细信息

冲突在工程项目中难以避免,从心理角度分析冲突归因及冲突方的调节焦点如何影响其冲突解决行为。应用多元层次回归分析法分析了368份由工程项目从业人员填写的有效问卷,以检验提出的研究假设。结果表明,对冲突的内在归因促进冲突方采取合作式冲突解决行为,而稳定性归因会抑制冲突方采取合作式冲突解决行为;对冲突的稳定性归因或可控性归因均会促使冲突方采取竞争式冲突解决行为;促进调节焦点对采取合作式、竞争式冲突解决行为有促进作用,防御调节焦点对采取合作式冲突解决行为有促进作用。研究结果有助于工程项目管理者基于冲突归因和调节焦点选择恰当的冲突解决行为,以及向有利方向影响对方的冲突归因。

关键词：工程冲突冲突解决行为冲突归因调节焦点

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油菜籽双螺旋压榨出油率的影响因素分析

武汉轻工大学学报 2024年第2期43卷 85-90页

作者：郑立焓宋少云黄涛甘维睿胡志刚武汉轻工大学机械工程学院武汉430023 武汉粮农机械制造有限公司武汉430070

为探究榨油生产线上影响油菜籽出油率的关键因素,对油菜籽的双螺旋压榨进行了实验分析,探索了油菜籽种类、物料含水率、主轴转速和轧胚机间隙大小对出油率的影响,并对结果进行了方差分析。研究结果表明:轧胚机间隙大小对出油率的影响显... 详细信息

为探究榨油生产线上影响油菜籽出油率的关键因素,对油菜籽的双螺旋压榨进行了实验分析,探索了油菜籽种类、物料含水率、主轴转速和轧胚机间隙大小对出油率的影响,并对结果进行了方差分析。研究结果表明:轧胚机间隙大小对出油率的影响显著,产地和含水率以及主轴转速的影响不显著,其因素主次顺序为轧胚机间隙大小>油菜籽产地>油菜籽含水率>主轴转速;对SSYZ120/12双螺旋榨油机而言,最佳的物料含水率为6%,轧胚机间隙为3 mm,最佳主轴转速为16 r/min。

关键词：油菜籽双螺旋榨油机实验研究出油率

精准对靶喷洒背景下作业小区地理坐标的计算方法

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江西农业大学学报 2024年第3期46卷 656-662页

作者：李志勇邓继忠赵高源张子超林芳源刘理涵马俊杰黄旭华南农业大学工程学院广东广州510642 国家精准农业航空施药技术国际联合研究中心广东广州510642 广东省江门市新会区农业农村局广东江门529100

【目的】确定作业小区的地理坐标是植保无人机实施精准对靶喷洒的关键问题之一。假如需要喷洒的作业小区数量众多或者需要喷洒的作业小区为水田等类型,可采用传统的人工在田间逐点测量的方法,则存在着工作量大、测量步骤繁琐、测量区域... 详细信息

【目的】确定作业小区的地理坐标是植保无人机实施精准对靶喷洒的关键问题之一。假如需要喷洒的作业小区数量众多或者需要喷洒的作业小区为水田等类型,可采用传统的人工在田间逐点测量的方法,则存在着工作量大、测量步骤繁琐、测量区域不易进入等缺点。因此,利用测量位于作业区域边界处或边界外有限数量位置点的地理坐标,然后按照一定算法计算出各作业小区的地理坐标就将克服前述的缺点,有着很大的实际应用价值,将极大提高精准对靶喷洒的效率和应用。【方法】根据实施精准对靶喷洒作业小区的地理坐标的求解需求,在测量学中找到一种地面点相对位置求取方法,同时将该方法引入并加以改进,分别提出了基于假定地球为正球体情况下的几何法和基于假定地球为椭球体情况下的投影法通过计算得到作业小区的地理坐标的2种算法,并且通过试验检验和比较2种算法的精度、求解速度、求解复杂度。【结果】通过试验可知,2种算法计算得到作业小区的地理坐标与实测值相比,误差在厘米级范围内,均符合实施精准对靶喷洒对精度的要求;在求解速度方面,几何法比投影法更加快速;在求解复杂度方面,几何法优于投影法。【结论】求解作业小区的地理坐标的2种算法-几何法和投影法在精度方面均可以满足实施精准对靶喷洒时对精度的需求。但是,在使用2种算法分别求解作业小区的地理坐标的过程中发现,在计算复杂度方面和计算速度方面,几何法相对于投影法更优、更简便、更适合实施精准对靶喷洒时对作业小区地理坐标的求解。

关键词：植保无人机作业小区地理信息坐标几何高斯-吕克投影精准对靶喷洒

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