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The Cloning and Fluorescence In situ HybridizationAnalysis of Cotton Telomere Sequence

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Journal of Integrative Agriculture 2012年第9期11卷 1417-1423页

作者： LING Jian CHENG Hua LIU Fang SONG Guo-li wang Chun-ying LI Shao-hui ZHANG Xiang-di wang Yu-hong wang kun-bo State Key Laboratory of Cotton Biology~Cotton Research Institute Chinese Academy of Agricultural Sciences Anyang 455000 P.R.China Anyang Institute of Technology Anyang 455000 P.R.China

Telomeres form the ends of eukaryotic chromosomes and serve as protective caps that keep chromosomes structure independency and completeness. The first plant telomere DNA was isolated from Arabidopsis thaliana and was shown to have tandemly repeated sequence 5-TTTAGGG-3： The Arabidopsis-type telomere has been found in many plants, but several reports indicate that this sequence is absent in some plants. Up to now, no research has been conducted on the telomere of cotton. In this paper, the Arabidopsis-type telomere sequence was amplified and cloned using the primers designed based on the fragment containing telomere sequence in an Arabidopsis bacterial artificial chromosome （BAC）. Fluorescence in situ hybridization （FISH） with cotton metaphase chromosomes using the Arabidopsis-type telomere sequence as probes indicated that the signals were located at all chromosome ends of seven diploid and two tetraploid cotton species with different signal intensities among chromosome complements of different cotton species, even between long and short arms of the same chromosome. To identify the signals of FISH, the genome DNA of Xinhai 7, a cultivar of Gossypium barbadense, digested by BAL-31 nuclease was introduced in this study. The result of BAL-31 digestion indicated that the hybridization signals of FISH represent the outermost DNA sequence of each cotton chromosomes. So we first proved that the telomeric repeats of cotton cross-hybridize with that of Arabidopsis. The results of terminal restriction fragment （TRF） showed significant variation in telomere length among cotton species. The telomere length of cultivated cotton was close to 20 kb and was larger than those of wild cotton species whose telomere length rahged from 6 to 20 kb.

关键词： cotton fluorescent in situ hybridization (FISH) telomere terminal restriction fragment (TRF)

Genome-wide assessment of genetic diversity and fiber quality traits characterization in Gossypium hirsutum races

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Journal of Integrative Agriculture 2017年第11期16卷 2402-2412页

作者： Kiflom Weldu Okubazghi LI Xiao-na CAI Xiao-yan wang Xing-xing CHEN Hao-dong ZHOU Zhong-li wang Chun-ying wang Yu-hong LIU Fang wang kun-bo State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences Anyang 455000 P.R.China Hamelmalo Agricultural College Keren P. O. Box 397 Eritrea Cotton Sciences Research Institute of Hunan/National Hybrid Cotton Research Promotion Center Changde 415101 P.R. China

Gossypium hirsutum races are believed to be potential reservoirs of desirable traits, which can play crucial roles to overcome the existing narrow genetic base of modern Upland cotton cultivars. However, prior to utilizing the races in cotton improve- ment programs, understanding their genetic constitutions is needed. Thus, this study used molecular and morphological techniques to characterize 110 G. hirsutum germplasm including 109 semi-wild accessions and one Upland cotton cultivar, CRI12. In the study, 104 SSR markers detected 795 alleles, with an average of 7.64 alleles per marker, ranging from 3 to 14, and average polymorphism information content （PIC） value of 0.71. And 96 of the markers were found to be highly informative, with PIC value〉0.50. Pairwise genetic similarity coefficient across the accessions ranged from 0.19 to 1.00, with an average value of 0.46. Morphological characterization was done using fiber length, fiber strength, micronaire, fiber uniformity index, and fiber elongation. Pairwise taxonomic distance within the accessions ranged from 0.17 to 3.41, with a mean of 1.33. The SSR and fiber quality traits data set based unweighted pair group method of arithmetic mean （UPGMA） analysis grouped the accessions into 7 and 12 distinct clusters, respectively, that corresponds well with the results of prin- cipal component analysis （PCA）. Our study revealed the existence of vast molecular and morphological diversities within the accessions and provided valuable information on each semi-wild accession for quick and better informed germplasm utilization in cotton breeding programs.

关键词： semi-wild accessions Gossypium hirsutum SSR markers genetic similarity taxonomic distance

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野生棉的收集与保存

棉花学报 2007年第5期19卷 354-361页

作者：王坤波农业部棉花遗传改良重点开放实验室中国农业科学院棉花研究所河南安阳455000

从种质资源角度,对我国野生棉的引种和保存工作做了较全面的总结,肯定了许多专业研究机构和工作者的历史贡献。作者认为,全部收集并保存到所有野生棉种的工作难度是很大的,但在材料类型方面还有很大的潜力。今后收集的重点是我国现在所... 详细信息

从种质资源角度,对我国野生棉的引种和保存工作做了较全面的总结,肯定了许多专业研究机构和工作者的历史贡献。作者认为,全部收集并保存到所有野生棉种的工作难度是很大的,但在材料类型方面还有很大的潜力。今后收集的重点是我国现在所缺乏的黄褐棉、二倍体栽培种的多年生类型等,考察收集重点地区是澳大利亚、巴西、印度、巴基斯坦、非洲部分地区等。从综合背景来看,美国仍然是当今补充收集最有效的对象。作者认为,我国进行种间杂交转育野生棉有益种质有巨大的潜在优势,而且我国已经到了发挥这个巨大优势的时候了。

关键词：野生棉资源收集保存

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论棉花永久性群体种类特色与应用价值

棉花学报 2000年第1期12卷 40-44页

作者：王坤波中国农科院棉花所

本文依据棉花多年生和半配生殖的属性和特性 ,以 H群体和细胞学标记群体为重点 ,较全面地论述了棉花永久性群体的种类特色、应用价值和构建途径 ,结合我国棉花资源与海南气候特点 ,讨论了我国在棉花永久性群体方面的工作优势。

关键词：棉花永久性群体 H群体细胞学标记群体

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论二元经济与区域共同富裕

特区经济 2011年第4期 144-145页

作者：王子坤孙波青岛理工大学山东青岛266033

本文梳理回顾了邓小平区域经济发展战略思想及其研究现状,阐述了二元经济理论的一般观点,试图以邓小平区域经济发展战略为基点,探讨二元经济理论之于解决区域经济共同富裕问题的损益。

关键词：邓小平二元经济共同富裕

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光收发组件驱动电路集成化技术研究

导航与控制 2011年第2期10卷 26-29页

作者：王坤博北京航空航天大学微纳测控与低维物理教育部重点实验室(筹)

本文对干涉式光纤陀螺用光收发组件的驱动电路集成化技术进行了研究。分析了光源输出光功率稳定性对光纤陀螺精度的影响。建立了光源驱动电路的理论模型。设计了相对简单、集成化程度高的光源驱动电路。对所研制的电路进行了全温测试。... 详细信息

本文对干涉式光纤陀螺用光收发组件的驱动电路集成化技术进行了研究。分析了光源输出光功率稳定性对光纤陀螺精度的影响。建立了光源驱动电路的理论模型。设计了相对简单、集成化程度高的光源驱动电路。对所研制的电路进行了全温测试。实现了光源驱动电路的高精度、集成化和小型化。

关键词：光纤陀螺光收发组件驱动电路集成化技术

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液化天然气罐箱的修理改造

石油化工设备 2009年第B08期38卷 23-24页

作者：王琨博新疆维吾尔自治区特种设备检验研究院新疆乌鲁木齐830000

介绍了对某液化天然气罐箱受损外壳进行的修复处理情况。

关键词：罐箱 LNG 维修改造

Loss-of-function mutations of microRNA-142-3p promote ASH1L expression to induce immune evasion and hepatocellular carcinoma progression

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World Journal of Gastroenterology 2025年第1期31卷 126-145页

作者： Xing-Hui Yu Yan Xie Jian Yu kun-Ning Zhang Zhou-bo Guo Di wang Zhao-Xian Li Wei-Qi Zhang Yu-Ying Tan Li Zhang Wen-Tao Jiang School of Medicine Nankai UniversityTianjin 300192China Tianjin Key Laboratory of Molecular Diagnosis and Treatment of Liver Cancer Tianjin First Center HospitalTianjin 300192China Department of Liver Transplantation Tianjin First Center HospitalTianjin 300192China First Central Clinical School Tianjin Medical UniversityTianjin 300192China

BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated *** therapeutic targets are beneficial to treat and predict the ***,the exact molecular mechanisms leading to the progression of HCC are still *** has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor *** this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between *** To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC *** In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC ***,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,*** statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank *** In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival ***1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated ***,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via *** the exploration mechanism,we found ASH1L may promote an immunosuppressive mi

关键词： Hepatocellular carcinoma MicroRNA-142-3p ASH1L Immune evasion Tumor immune microenvironment Apoptosis

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从美国棉花带会议谈中国棉花学会年会

中国棉花 2013年第4期40卷 1-5页

作者：王坤波中国农业科学院棉花研究所/棉花生物学国家重点实验室河南安阳455000

作者参加了美国2013年棉花带会议,对该年会的简历、主办机构、参会代表、会议内容、开会形式和会议费用等,作了较全面的了解。简单介绍了中国棉花学会的年会,比较了2个年会的异同点。从会议组织者的角度,对如何进一步办好中国棉花学会... 详细信息

作者参加了美国2013年棉花带会议,对该年会的简历、主办机构、参会代表、会议内容、开会形式和会议费用等,作了较全面的了解。简单介绍了中国棉花学会的年会,比较了2个年会的异同点。从会议组织者的角度,对如何进一步办好中国棉花学会的年会提出了建议。

关键词：美国棉花带会议中国棉花学会年会异同点