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The photodamage process of pigments and proteins of PSI complexes from Spinacia Oleracea L.

Chinese Science Bulletin 2001年第21期46卷 1812-1816页

作者： WEI Jie YU Hui LI Liangbi KUANG Tingyun wang jushuo GONG Yandao ZHAO Nanming Laboratory of Photosynthesis Basic Research Institute of BotanyChinese Academy of SciencesBeijing 100093China Department of Biological Science and Biotechnology Tsinghua UniversityBeijing 100084 China

Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal ab-sorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals con-tributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reaction

关键词： PSI pigment-protein complex photodamage LHCI absorption spectra CD spectra.

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The excitation energy transfer between β-Car and Chla molecules in PS Ⅱ core antenna complexes CP43 and CP47

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Chinese Science Bulletin 2000年第17期45卷 1579-1583页

作者： SHAN Jixiu, wang jushuo, LI Liangbi,ZHAO Nanming & KUANG Tingyun1. Laboratory of Photosynthesis Basic Research, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2. Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, ChinaCorrespondence should be addressed to Kuang Tingyun Laboratory of Photosynthesis Basic Research Institute of Botany Chinese Academy of Sciences Beijing China Department of Biological Sciences and Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The excitation energy transfer efficiency between p-Car and Chla molecules in purified CP43 and CP47 was calculated by comparing absorption and fluorescence excitation after normalization at 550 nm, CP43 had an energy transfer efficiency of 29.1% while the CP47 had an energy transfer efficiency of 62.8%, proving that excitation energy was transferred between p-Car and Chla molecules in CP43 and CP47 at normal conditions. The excitation energy transfer between p-Car and Chla molecules in CP43 and CP47 may occur through the "Dexter" mechanism and the distance between these two kinds of pigments should be less than 1 nm. In addition, the results were also used to discuss the conformational relationship between p-Car and Chla molecules in CP43 and CP47.

关键词： CP43 CP47 β-Car Chla excitation energy transfer.

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海安方言“说的”表义功能研究

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中国语文 2018年第3期 323-329,383页

作者：汪如东上海财经大学国际文化交流学院

海安方言中"说的_1"表言说,与"说"在使用时有交叉,也有结构、意义上的不同。"说的_2"表传闻,相当于"据说",与"说的_1"存在功能上的差异;"说的_3"表传情,分别表示对既成事实或行为表示吃惊和不满、确认或申明原有观点、恍然大悟等多种语... 详细信息

海安方言中"说的_1"表言说,与"说"在使用时有交叉,也有结构、意义上的不同。"说的_2"表传闻,相当于"据说",与"说的_1"存在功能上的差异;"说的_3"表传情,分别表示对既成事实或行为表示吃惊和不满、确认或申明原有观点、恍然大悟等多种语气;"说的_4"表假设,主要充当假设/转折及引入话题的功能,"说的_3""说的_4"可归为表"惊异"义的功能范畴。

关键词：海安方言 “说的” 言说传闻惊异

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