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Arbuscular mycorrhizal fungi ameliorate the chemical properties and enzyme activities of rhizosphere soil in reclaimed mining subsidence in northwestern China

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Journal of Arid Land 2019年第1期11卷 135-147页

作者： QIU Lang BI Yinli JIANG Bin wang zhigang ZHANG Yanxu Yryszhan ZHAKYPBEK State Key Laboratory of Coal Resources and Safe Mining China University of Mining and Technology (Beijing) College of Geoscience and Surveying Engineering China University of Mining and Technology (Beijing) Satbayev University

In semi-arid region of northwestern China, underground mining subsidence often results in decreased vegetation coverage, impoverishment of soil fertility and water stress. In addition, the physical-chemical and biological properties of soil also change, resulting in more susceptible to degradation. In particular, subsidence causes disturbance of the symbioses of plant and microbe that can play a beneficial role in the establishment of vegetation communities in degraded ecosystems. The objective of this study was to evaluate the effects of revegetation with exotic arbuscular mycorrhizal fungi(AMF) inoculum on the chemical and biological properties of soil over time in mining subsidence areas. Soils were sampled at a depth up to 30 cm in the adjacent rhizosphere of Amorpha fruticose Linn. from five reclaimed vegetation communities in northwestern China. In August 2015, a field trial was set up with five historical revegetation experiments established in 2008(7-year), 2011(4-year), 2012(3-year), 2013(2-year) and 2014(1-year), respectively. Each reclamation experiment included two treatments, i.e., revegetation with exotic AMF inoculum(AMF) and non-AMF inoculum(the control). Root mycorrhizal colonization, glomalin-related soil protein(GRSP), soil organic carbon(SOC), soil nutrients, and enzyme activities were also assessed. The results showed that mycorrhizal colonization of inoculated plants increased by 33.3%–163.0% compared to that of non-inoculated plants(P<0.05). Revegetation with exotic AMF inoculum also significantly improved total GRSR(T-GRSP) and easily extracted GRSP(EE-GRSP) concentrations compared to control, besides the T-GRSP in 1-year experiment and the EE-GRSP in 2-year experiment. A significant increase in SOC content was only observed in 7-year AMF reclaimed soils compared to non-AMF reclaimed soils. Soil total N(TN), Olsen phosphorus(P) and available potassium(K) were significantly higher in inoculated soil after 1–7 years of reclamation(except for in

关键词： revegetation mycorrhizal colonization glomalin-related soil proteins arbuscular mycorrhizal fungi coal mining Amorpha fruticose

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Fate of secondary effluent dissolved organic matter during soil-aquifer treatment

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Chinese Science Bulletin 2007年第18期52卷 2496-2505页

作者： XUE Shuang ZHAO QingLiang WEI LiangLiang wang LiNa LIU zhigang School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin 150090 China

The reduction of mass and trihalomethane formation potential (THMFP) of dissolved organic matter (DOM) and its fractions from secondary effluent during laboratory-scale soil-aquifer treatment (SAT) soil columns were studied. Reduction in dissolved organic carbon (DOC), absorbance of ultraviolet light at 254 nm (UV-254), biodegradable dissolved organic carbon (BDOC) and nonbiodegradable dis- solved organic carbon (NBDOC) for the bulk DOM averaged 72.35%, 53.98%, 97.49% and 35.33% across the soil columns, respectively. Using XAD-8 and XAD-4 resins, DOM was fractionated into 3 fractions: hydrophobic acid (HPO-A), transphilic acid (TPI-A) and hydrophilic fraction (HPI). HPO-A was removed by 61.06%, TPI-A by 54.86% and HPI by 74.95% as DOC as a consequence of the laboratory-scale SAT, respectively. The reduction of THMFP from HPO-A, TPI-A and HPI was 27.24%, 26.24% and 36.08%, respectively. Proton nuclear magnetic resonance (1H-NMR) spectra revealed that the HPO-A isolated from the secondary effluent contained more aromatic functional groups than the corresponding TPI-A. Fourier-transform infrared (FT-IR) spectrum analysis illustrated that TPI-A had decreased hydrocarbon and increased aromatics content in the SAT columns. Specific ultraviolet light absorbance (SUVA) and specific THMFP for each DOM fraction increased across the soil columns and HPI exhibited greater increase in both than HPO-A and TPI-A. The most problematic THM precursor was found to be HPO-A with its high quantity present in recharged water and high chlorine reactivity.

关键词：溶解有机物生物降解能力三卤代甲烷封款式污染

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Microstructure, mechanical and thermo-physical properties of hot-pressed Al2O3-GdAlO3-ZrO2 ceramics with eutectic composition

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Progress in Natural Science:Materials International 2017年第4期27卷 491-497页

作者： Abdelkhalek Henniche Jiahu Ouyang Yonghui Ma zhigang wang Zhanguo Liu Yujin wang Mehdi Derradji Xinyu Liu School of Materials Science and Engineering Harbin Institute of Technology Laboratoire Génie des Matériaux Ecole Militaire Polytechnique Institute of Composite Materials Key Laboratory of Superlight Material and Surface Technology of Ministry of Education College of Materials Science and Chemical Engineering Harbin Engineering University

A low cost chemical co-precipitation method was employed to fabricate nanoscale Al2O3-GdAlO3-ZrO2powder with eutectic composition. A careful control of reaction conditions was required during the preparation. The synthesized nanopowders exhibited a particle size of 20-200 nm, and were highly dispersive and uniform. The results showed that calcination temperature had an important influence on the phase constituents of the nanopowders. With increasing the calcination temperature, a phase transformation from θ-Al2O3to α-Al2O3and a thermal decomposition from Gd3Al5O12(GdAG) to GdAlO3and α-Al2O3occurred in sequence. A calcination temperature of 1300 ℃ was needed for the crystallization of α-Al2O3. These nanosized powders were consolidated via hot pressing to produce a fully densified ceramic composite with eutectic composition. The Al2O3-GdAlO3-ZrO2ceramic hot-pressed at 1500 ℃ exhibited a relative density of 99.4%, a flexural strength of 485 MPa and a fracture toughness of 6.5 MPa m1/2. The ceramic had a thermal conductivity of 1.9 W m K-1at 1200 ℃ and a thermal expansion coefficient of 9.49 ×10-6K-1at 1100 ℃.

关键词： Al2O3-GdAlO3-ZrO2 system Co-precipitation hot pressing Flexural strength thermal conductivity

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Ilizarov骨搬移联合抗生素骨水泥促进胫骨大段骨缺损的对接点愈合

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中国组织工程研究 2025年第10期29卷 2038-2043页

作者：张治博王兆林王志刚李朋姜建浩张锴杨淑野杜刚强滨州医学院附属医院创伤骨科山东省滨州市256603

背景:Ilizarov骨搬移治疗开放性胫骨大段骨缺损十分有效,但仍有并发症,其中对接点愈合困难是治疗难点之一。目的:探讨Ilizarov骨搬移联合抗生素骨水泥对开放性胫骨大段骨缺损术后对接点愈合的影响。方法:选择2010年8月至2022年1月滨州... 详细信息

背景:Ilizarov骨搬移治疗开放性胫骨大段骨缺损十分有效,但仍有并发症,其中对接点愈合困难是治疗难点之一。目的:探讨Ilizarov骨搬移联合抗生素骨水泥对开放性胫骨大段骨缺损术后对接点愈合的影响。方法:选择2010年8月至2022年1月滨州医学院附属医院收治的开放性胫骨大段(骨缺损> 4 cm)骨缺损患者51例,其中28例接受单纯Ilizarov骨搬移治疗(对照组),23例接受Ilizarov骨搬移联合抗生素骨水泥治疗(试验组)。统计比较两组患者外固定时间、骨愈合时间、骨愈合指数、骨搬移过程中的目测类比评分、末次随访时的骨缺损肢体功能、对接点愈合及并发症发生情况。结果与结论:(1)51例患者均获得完整随访,平均随访(22.53±5.77)个月,试验组患者外固定时间、骨愈合时间、骨愈合指数、术后感染率及对接点未愈合率均少于对照组(P 0.05);(2)结果表明,相较于单纯的Ilizarov骨搬移治疗,Ilizarov骨搬移联合抗生素骨水泥治疗可促进开放性胫骨骨折对接点的愈合,提高骨愈合率。

关键词：膜诱导成骨技术骨搬移对接点骨愈合胫骨开放性骨折大段骨缺损

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改进型止浆塞在上斜钻孔带压封堵地质异常区中的应用研究

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煤炭技术 2025年第1期44卷 167-170页

作者：王志刚马丁杨震贾帆帆山西天润煤化集团德通煤业有限公司山西临汾041000 中煤科工西安研究院(集团)有限公司西安710077

为解决煤矿井下地质探查上斜钻孔带压封堵异常区时,漏浆、跑浆及加压管磨损破裂等问题,设计出带有锥形头、单向阀和内置导流管的改进型止浆塞,并在山西某矿进行了现场试验。试验对7个钻孔下入孔底止浆塞,均达到设计下入深度,平均下深191... 详细信息

为解决煤矿井下地质探查上斜钻孔带压封堵异常区时,漏浆、跑浆及加压管磨损破裂等问题,设计出带有锥形头、单向阀和内置导流管的改进型止浆塞,并在山西某矿进行了现场试验。试验对7个钻孔下入孔底止浆塞,均达到设计下入深度,平均下深191 m,平均膨胀压力5.1 MPa,合格率100%,下入过程中未出现加压管磨损或断裂事故。对孔内有毒有害气体含量偏高的2^(#)、6^(#)、7^(#)钻孔进行持续观测,发现在下入止浆塞后,孔口有毒有害气体含量均呈不同程度衰减趋势,其中H_(2)S气体含量分别衰减40%、33%、13%;CO气体含量分别衰减20%、70%、25%;CH_(4)气体含量分别衰减0.3%、4.4%、10%。试验结果表明,改进型止浆塞结构设计合理,能够按要求下入指定孔深,且封堵效果良好,孔内有毒有害气体得到有效隔绝,保证了巷道掘进及采面回采安全高效进行。

关键词：止浆塞上斜钻孔封堵地质异常区应用

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Effect of Nozzle Inclination Angle on Fuel-Air Mixing and Combustion in a Heavy Fuel Engine

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Fluid Dynamics & Materials Processing 2024年第2期20卷 365-382页

作者： zhigang wang Bin Zheng Peidong Zhao Baoli wang Fanyan Meng Wenke Xu Jian Meng School of Transportation and Vehicle Engineering Shandong University of TechnologyZibo255049China

Heavy-fuel engines are widely used in UAVs(Unmanned Autonomous Vehicles)because of their reliability and high-power *** this study,a combustion model for an in-cylinder direct injection engine has been imple-mented using the AVL FIRE *** effects of the angle of nozzle inclination on fuel evaporation,mixture distribution,and combustion in the engine cylinder have been systematically studied at 5500 r/min and consider-ing full load cruise *** to the results,as the angle of nozzle inclination increases,the maximum combustion explosion pressure in the cylinderﬁrst increases and then it *** the angle of nozzle incli-nation is less than 45°,the quality of the mixture in the cylinder and the combustion performance can be improved by increasing the *** the angle of nozzle inclination is greater than 45°,however,the mixture unevenness increases slightly with the angle,leading to a deterioration of the combustion *** the angle of nozzle inclination is between 35°and 55°,the overall combustion performance of the engine is rela-tively *** the angle of nozzle inclination is 45°,the combustion chamber’s geometry and the cylinder’s airﬂow are well matched with the fuel spray,and the mixture quality is the *** with 25°,the peak heat release rate increases by 20%,and the maximum combustion burst pressure increases by 5.5%.

关键词： Aviation heavy fuel piston engine nozzle inclination angle combustion fuel-air mixing

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Efficient synthesis of functionalized 2-pyridones by ytterbium chloride catalyzed tandem condensation

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Chinese Science Bulletin 2012年第14期57卷 1612-1615页

作者： wang QianNan YAO zhigang XU Fan SHEN Qi Key Laboratory of Organic Synthesis College of Chemistry Chemical Engineering and Materials Science Soochow University Suzhou 215123China

An efficient method was developed for the synthesis of arylidene heterobicyclic 3-cyano-2-pyridones via ytterbium chloride catalyzed tandem condensation of aromatic aldehydes,cyclic ketones,and *** conditions and scop... 详细信息

关键词：高效合成氯化物吡啶串联催化冷凝镱反应机制

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In vivo transfection of enhanced green fluorescent protein in rat retinal ganglion cells mediated by ultrasound-induced microbubbles

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Neural Regeneration Research 2009年第6期4卷 413-417页

作者： Hong Su Su Liu zhigang wang Wenyue Xie Bing Jiang Haibo Xiong Department of Ophthalmology Second Hospital of Chongqing Medical University Chongqing 400010 China Institute of Ultrasonic Imaging Chongqing Medical University Chongqing 400010 China

BACKGROUND： Studies have demonstrated that ultrasound-mediated microbubble destruction significantly improves transfection efficiency of enhanced green fluorescent protein （EGFP） in in vitro cultured retinal ganglial cells （RGCs）. OBJECTIVE： To investigate the feasibility of ultrasound-mediated microbubble destruction for EGFP transfection in rat RGCs, and to compare efficiency and cell damage with traditional transfection methods. DESIGN, TIME AND SETTING： In vivo, gene engineering experiment. The study was performed at the Central Laboratory, Institute of Ultrasonic Imaging, Chongqing Medical University from March to July 2008. MATERIALS： Eukaryotic expression vector plasmid EGFP and microbubbles were prepared by the Institute of Ultrasonic Imaging, Chongqing Medical University. The microbubbles were produced at a concentration of 8.7 × 10^11/L, with a 2-4 μm diameter, and 10-hour half-life in vitro. METHODS： A total of 50 Sprague Dawley rats were randomly assigned to four groups. Normal controls （n = 5） were infused with 5 μL normal saline to the vitreous cavity; the naked plasmid group （n = 15） was infused with 5 pL EGFP plasmid to the vitreous cavity; in the plasmid with ultrasound group （n = 15）, the eyes were irradiated with low-energy ultrasound wave （0.5 W/cm^2） for a total of 60 seconds （irradiated for 5 seconds, at 10-second intervals） immediately following infusion of EGFP plasmids to the vitreous cavities. In the microbubble-ultrasound group （n = 15）, the eyes were irradiated with the same power of ultrasonic wave immediately following infusion of microbubbles containing EGFP plasmids to the vitreous cavities. MAIN OUTCOME MEASURES： After 7 days, retinal preparations and EGFP expression in RGCs were observed by fluorescence microscopy. RGC quantification in the retinal ganglion cell layer was performed. In addition, EGFP mRNA expression was semi-quantitatively determined by RT-PCR. RESULTS： The transfection efficiency of EGFP to RGCs by microbubbles wit

关键词： ultrasound contrast agent microbubble retinal ganglion cells in vivo gene therapy enhanced green fluorescent protein

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Tumor necrosis factor-alpha(TNF-α) in spotted halibut Verasper variegatus at the embryonic and metamorphic stages

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Journal of Oceanology and Limnology 2020年第2期38卷 454-466页

作者： LI Zan LIU Xiumei DU Xinxin ZHANG Kai CHEN Yan wang Xubo wang zhigang YU Haiyang ZHANG Quanqi School of Agriculture Ludong UniversityYantai 264025China College of Life Sciences Yantai UniversityYantai 264005China Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life ScienceOcean University of ChinaQingdao 266003China

As an aquatic fish,the spotted halibut Verasper variegatus is highly susceptible to bacterial and virus *** necrosis factor-alpha(TNF-α)as a cytokine could control the inflammatory *** functions of TNF-αin many species have been widely studied,particularly in ***,little is known about the TNF-αfunctions in *** first cloned and sequenced the TNF-αgene in ***(VvTNF-α).The two conserved cysteine residues,transmembrane sequence,Thr-Leu motif,and TNF family signature,as well as the TA-rich motifs of its proteins related to inflammatory responses had high similarity to those of the other teleost and mammalian TNF-α.The phylogenetic analysis showed that VvTNF-αwas consistent with TNF-αgenes of other *** VvTNF-αtranscripts were extensively distributed in the peripheral blood leukocytes(PBLs),spleen,and gill,indicating that the VvTNF-αhad a role in immune ***,treatment with pathogen-associated molecular patterns(PAMPs)could induce a rapid and significant increase of VvTNF-αin the PBLs,which reveals that VvTNF-αdoes participate in the host immune responses against bacterial and viral *** found that VvTNF-αhad an interesting expression pattern during metamorphosis,showing that the flatfish TNF-αmay have some novel functions during specific developmental *** addition,the 3 D structure prediction of VvTNF-αprovided an indication of how it is likely to interact with other ***,VvTNF-αhas multiple functions,and provides valuable information to explore novel functions of TNF-α.

关键词： tumor necrosis factor-alpha(TNF-α) Verasper variegatus metamorphosis peripheral blood leukocytes(PBLs) 3D modeling pathogen-associated molecular patterns(PAMPs)

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Fuzzy Assessment Method and Its Application to Selecting Project Managers

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Petroleum Science 2006年第4期3卷 63-66页

作者： Sun Renjin wang zhigang wang Li School of Business Administration China University of Petroleum Beijing 102249 China

Open competition is a new form of the assessment of candidates and selection of project managers. This has many merits compared to the traditional administrative method of appointment. This article introduces a method of fuzzy assessment of project manager candidates. Fuzzy assessment unifies objective qualitative and quantitative appraisal and can be used for improving decision-making in the selection process.

关键词： Fuzzy assessment project manager selection process

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