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Vitis amuerensis CBF3 Gene Isolation,Sequence Analysis and Expression

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Agricultural Sciences in China 2010年第8期9卷 1127-1132页

作者： wang Zhan-bin FENG Lian-rong wang Jin-jie wang zhi-ying Northeast Forestry University/Key Labotatory of Forest Tree Genetic Improvement and Biotechnology Ministry of Agricuture Harbin 150040 P.R.China Institute of Poplar of Liaoning Province Gaizhou 115200 P.R. China

The full length cDNA sequence of CBF3 （CRT/DRE-binding factor） was cloned from Vitis amurensis by reverse transcription polymerase chain reaction （RT-PCR） using the primers designed based on CBF genes available in GenBank. Sequence analysis showed that the gene had 854 bp long and its coding sequence contained 720 bp, encoding a protein with 239 amino acids and an AP2 structural domain. The molecular mass of CBF3 was predicted to be 25.9 kDa and its theoretical isoelectric point was 7.02 and aliphatic index was 59.29. The average hydropathicity of the protein was -0.551. The tertiary structures of CBF3 were also analyzed. The prokaryotic expression vector pGEX-4T-CBF3 containing CBF3 gene was constructed and CBF3 fusion protein （52 kDa） was produced in Escherichia coli after induction with 1 mmol L-1 IPTG. Further studies are needed to evaluate its potential application for improving plant resistance to cold and other stress condition such as drought and salinity.

关键词： Vitis amurensis CBF gene cloning sequence analysis expression in prokaryote

Preparation of dry flowable formulations of Clonostachys rosea by spray drying and application for Sclerotinia sclerotiorum control

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Journal of Integrative Agriculture 2018年第3期17卷 613-620页

作者： WU Hong-qu SUN Li-li LIU Fang wang zhi-ying CAO Chuan-wang College of Forestry Northeast Forestry University Harbin 150040 P.R.China Hubei Biopesticide Engineering Research Centre Wuhan 430064 P.R.China

A dry flowable formulation of Clonostachys rosea with fungicidal activity against Sclerotinia sclerotiorum was prepared by spray drying. The formulation was optimized by a four-factor, three-level orthogonal experiment to screen inert ingredients and spray-drying conditions. The optimal dry flowable formulation of C. rosea included 30% C. rosea （ratio of conidia powder and its fermentation broth is 1：3）, 3% Morwet EFW, 4% K12, 10% Morwet D425, 9% sodium salt of polynaphthalene sulphonic acid （NNO）, 5% croscarmellose sodium, 5% （NH4）2SO4, 0.5% carboxymethyl cellulose sodium （CMC-Na）, 1% oxalic acid and palygorskite （carrier） up to 100%. The formulation exhibited good physical characteristics, such as high dispersibility, viability and a long shelf life. Plate antagonism tests and pot trials indicated that the dry flowable formulation was very effective against S. sclerotiorum, with control efficiency of up to 88.30%. This dry flowable formulation of C. rosea is a new potential commercial fungicide for spray drying to control S. sclerotiorum.

关键词： Clonostachys rosea dry flowable formulation Sclerotinia sclerofiorum spray drying

Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella

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Journal of Integrative Agriculture 2014年第4期13卷 904-910页

作者： ZHOU Zuo-yong HU Shi-jun wang zhi-ying GUO zhi-li QIN Bo NIE Kui Department of Veterinary Medicine Rongchang Campus of Southwest University College of Animal Science and Technology Southwest University

Toll-like receptors （TLRs） are a group of highly conserved molecules which initiate the innate immune response to pathogens by recognizing structural motifs of microbes. Understanding the changes in chicken Toll-like receptors （ChTLRs） and signal adaptors expression that occur with Eimeria tenella infection will help to elucidate the molecular basis of immune control of coccidiosis caused by Eimeria. The present study detected the dynamic changes in the expression of ChTLRs and associated signal adaptors in the spleen and cecum ofE. tenella-infected chickens during the early stage of infection. The results showed that the expression peak for ChTLRs, MyD88 and TRIF occurred at 12 h post-infection （hpi）, ChTLR3, ChTLRI 5 and MyD88 mRNA expression in the spleen ofE. tenella infected chickens were significantly higher （P〈0.05） than that of negative control chickens, and there were similar tendencies of these molecules expression in the cecum and spleen of E. tenella-infected chickens. The expression of MyD88 was upregnlated at four time points in the cecum of E. tenella-infected chickens. The results of this study indicate that ChTLR3, ChTLR15 and MyD88 play a role in young chickens infected with E. tenella.

关键词： Eimeria tenella signal adaptors Toll-like receptors spleen and cecum chicken

Effect of tramadol on immune responses and nociceptive thresholds in a rat model of incisional pain

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第11期9卷 895-902页

作者： Yong-min LIU Sheng-mei ZHU Kui-rong wang zhi-ying FENG Qing-lian CHEN Department of Anesthesiology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Objective： To evaluate the effects oftramadol on the proinflammatory responses in a rat model of incisional pain by investigating its effects on nociceptive thresholds and serum interleukin-6 （IL-6） and IL-2 levels. Methods： Forty-two male Sprague-Dawley （SD） rats scheduled for plantar incision were randomly divided into 7 groups 01=6 in each group）. Rats in Group 1 receiving general anesthesia with no incision were served as control; At 30 min before skin incision, Groups 2-5 were given 5 ml normal saline or 1, 10, and 20 mg/kg tramadol, respectively, intraperitoneally （i.p.）; Group 6 received 10 mg/kg tramadol after operation; Group 7 received 10 mg/kg tramadol before incision, followed by 200 μg/kg naloxone after operation. Mechanical allodynia was measured by electronic yon Frey filament to evaluate the nociceptive thresholds 1 h before incision, and 1 h and 2 h after operation. Serum IL-6 and IL-2 levels were measured by enzyme-linked immunosorbent assay （ELISA） 2 h after operation. Results： Mechanical thresholds decreased significantly and serum IL-6 level increased significantly after operation in Group 2 compared with control （P〈0.01）, and these changes were reversed respectively by tramadol in a dose-dependent manner （P〈0.05 and P〈0.01, respectively）. IL-2 level remained unchanged after operation in Group 2, but decreased in Group 3 （P〈0.05）, then gradually returned to the normal level in Groups 4 and 5. The intraperitoneally injected tramadol （10 and 20 mg/kg） produced a potent and dose-dependent antinocicptive effect on the lesioned paw. The antinocicptive effects of tramadol were partially an- tagonized by naloxone （200 μg/kg）, suggesting an additional non-opioid mechanism. Conclusion： The results suggest that tramadol could be a good choice for the treatment of pain under the conditions that immunosuppression may be particularly contraindicated.

关键词： Tramadol Pain Mechanical allodynia lnterleukin-6 (IL-6) Interleukin-2 (IL-2) Rat

Analysis of misdiagnosis of lymphomas in oral, maxillofacial and cervical regions

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中国口腔颌面外科杂志 2008年第B5期6卷 168-169页

作者： wang zhi Xing Yan ying Bin Department of Oral & Maxillofacial Surgery TianjinStomatological Hospital Tianjin 300041China

在MIMO-OFDM系统中的空频最小化发射功率算法

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上海交通大学学报 2007年第5期41卷 739-742页

作者：王智鹰何晨上海交通大学电子工程系上海200240

在多输入多输出(MIMO)系统最小化发射功率自适应调制算法的基础上,针对实际的无线宽带信道,提出了适用于MIMO-OFDM空频二维最小化发射功率的自适应调制算法,并将其与均匀分配比特、功率的方案和空间最小化发射功率自适应调制方案进行比... 详细信息

在多输入多输出(MIMO)系统最小化发射功率自适应调制算法的基础上,针对实际的无线宽带信道,提出了适用于MIMO-OFDM空频二维最小化发射功率的自适应调制算法,并将其与均匀分配比特、功率的方案和空间最小化发射功率自适应调制方案进行比较.实验结果表明,所提出的空频二维自适应调制算法可以在保证系统目标传输速率和目标误比特率的前提下,进一步减小系统所需要的平均发射功率.

关键词：多输入多输出正交频分复用发射功率自适应调制

自适应调制MIMO系统的最小化发射功率准则

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电子与信息学报 2007年第4期29卷 950-953页

作者：王智鹰何晨上海交通大学电子工程系上海200240

该文提出了一种新的适用于多输入多输出系统的最小化发射总功率自适应调制准则,给出了其在完美信道反馈条件下最佳功率/比特分配方案的理论闭式解及非完美反馈时实现算法。最小化发射功率准则的显著优点是能够在满足用户需要的传输速率... 详细信息

该文提出了一种新的适用于多输入多输出系统的最小化发射总功率自适应调制准则,给出了其在完美信道反馈条件下最佳功率/比特分配方案的理论闭式解及非完美反馈时实现算法。最小化发射功率准则的显著优点是能够在满足用户需要的传输速率和误比特率的前提下,以尽可能小的发射功率实现数据传输,使无线网络中的跨层优化成为可能。

关键词：自适应调制(AM) 多输入多输出(MIMO) 服务质量(QoS)

义齿软衬材料在口腔修复中的应用及其组织相容性

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中国组织工程研究与临床康复 2009年第21期13卷 4157-4160页

作者：屈直王稚英辽宁医学院附属第二医院口腔修复科辽宁省锦州市121000 辽宁医学院附属第二医院口腔种植中心辽宁省锦州市121000

全口义齿修复中常能遇到一些疑难问题,例如牙槽嵴高度吸收后,支持骨受力不均或集中于局部;或者牙槽嵴呈刃状,其上黏膜过薄;戴义齿后义齿组织面看似密合,但有反复压痛;软硬组织倒凹大,义齿摘戴时基托边缘压迫产生疼痛;义齿下牙槽嵴受压... 详细信息

全口义齿修复中常能遇到一些疑难问题,例如牙槽嵴高度吸收后,支持骨受力不均或集中于局部;或者牙槽嵴呈刃状,其上黏膜过薄;戴义齿后义齿组织面看似密合,但有反复压痛;软硬组织倒凹大,义齿摘戴时基托边缘压迫产生疼痛;义齿下牙槽嵴受压不均匀造成义齿性口炎;义齿不密合产生压痛或固位力减低。义齿软衬材料做为粘附在基托基托组织面,固化后有一定弹性的义齿衬垫材料,很好的解决这些问题。文章介绍了义齿软衬材料在口腔修复中的应用与相容性研究。

关键词：义齿软衬材料生物相容性修复

Enhancing code density of transport triggered architecture through dual-mode computing

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通讯和计算机（中英文版） 2007年第7期4卷 22-26页

作者： HE Lei ZHAO Xue-mi wang zhi-ying School of Computer National University of Defense Technology Changsha 410073 China