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Lead isotopic study of Shang Dynasty bronzes (13-11 B.C.)and their ore sources

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Nuclear Science and Techniques 2000年第2期11卷 65-71页

作者： PEng Zi-Cheng ZHAng zhao-Feng HE Jian-Feng wang zhao-Rong(Department of Earth and Space Science, Univcrsity of Sciencc and Technology of china,Hefei 230026)liU Shi-Zhong(Jiangxi Historical Relic Archaeology Rescarch institute, Nanchang 330003)HUA Jue-Mi Department of Earth and Space Science University of Science and Technology of ChinaHefei 230026 Jiangxi Historical Relic Archaeology Research institute Nanchang 330003 Natural Science Historical institute the Chinese Academy of Sciences Beijing 100010

The Shang Dynasty civilization in china is based on the development of the bronze techniques. A large amount of Shang Dynasty bronzes, excavated from Jiangxi, Hubei and Henan Provinces, have become a focus of world attention. However,the Sha-ng Dynasty center was located at Zhenzhou and Allyang city areas, Henan Province, where no large copper ores have been found so far. Therefore, where did the huge ore material for casting the bronzes come from? It is an unsettled question paid attention by the archaeologists and scicntists. 35 Shang Dynasty bronzes and 21 copper and lead ore materials aged in the Shang-Zhou Dynasty, the Spring-Autumn Period and the modern time, have been measured by the use of mass *** upon lead isotopic ratios, the ore material for casting the bronzes with the middle isotopic ratios of 207Pb/206Pb ranged in 0.8～0.9 could come from the ancient copper mine of Tongling, Jiangxi Province and Tong Lushan, Hubei Province and that with the high isotopic ratios (＞0.9) could be from the northern part of the Shang Empire, called "gongfang" in the historical records, e.g. today’s Hebei and liaoningProvinces. The others with the low isotopic ratios (＜0.8) might originate from poly-metalic deposits, with the high isotopic ratios of 238U/204 Pb in the ore flux or in the magma.

关键词：商代青铜器铜铜矿石材料同位素比率考古商代文明来源

液相色谱-质谱联用测定乌头碱血药浓度及其药代动力学参数的方法学研究

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分析测试学报 2004年第Z1期23卷 51-53,56页

作者：王朝虹文蛟何毅公安部物证鉴定中心北京100038

　　乌头碱(aconitine)是存在于乌头属多种植物中的一种双酯二萜生物碱,具有镇[1]、抗炎[2]、抗癫痫[3]、抗肿瘤[4]、提高免疫功能[5]等多种生理活性,但因其具有强烈的心脏毒性和神经毒性,安全系数小,治疗剂量与中毒剂量接近(小鼠LD501.... 详细信息

　　乌头碱(aconitine)是存在于乌头属多种植物中的一种双酯二萜生物碱,具有镇[1]、抗炎[2]、抗癫痫[3]、抗肿瘤[4]、提高免疫功能[5]等多种生理活性,但因其具有强烈的心脏毒性和神经毒性,安全系数小,治疗剂量与中毒剂量接近(小鼠LD501.8mg/kg,op;人绝对致死量为2～5mg)[7],限制了对该化合物作为药用的进一步研究,目前仅将其作为致心率失常模型的工具药使用,尚未见其药代动力学的报道.在本文中首次应用液相色谱-质谱联用法对乌头碱在大鼠体内的药代动力学进行了研究.……

关键词： LC - MS Solid phase extraction Aconitine Pharmacokinetics

Development and optimization of an antibody array method for potential cancer biomarker detection

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The Journal of Biomedical Research 2011年第1期25卷 63-70页

作者： Shuangshuang wang Ping zhao Brian Cao Key Laboratory of Antibody Technology of Ministry of Health Nanjing Medical University Nanjing Jiangsu Province 210029 China Antibody Technology Laboratory Van Andel Research Institute Grand Rapids MI 49503 USA

Biomarkers play an important role in the detection at an early stage of pancreatic cancer. The aim of the present study was to optimize the conditions of antibody arrays for detecting Hippocalcin-like 1 （HPCAL1）, phosphatidylethanolamine binding protein 1 （PEBP1）, lectin galactoside-binding soluble 7 （LgALS7）, and serpin peptidase inhibitor clade E member 2 （SERPINE2） as biomarkers for pancreatic cancer detection in a single assay and to investigate antibodies’ specificity and cross-reactivity. Capture antibodies against HPCAL1, PEBP1, LgALS7 and SERPINE2 were printed on nitrocellulose coated glass slides. HPCAL1, PEBP1, LgALS7 and SERPINE2 proteins with different concentrations were incubated with the capture antibodies at different temperatures for different time periods. Biotinylated detection antibodies recognizing a different epitope on the captured proteins and a secondary detection molecule （Streptavidin-PE） were used to detect fluorescent signals. The arrays showed the strongest signals when the concentration of the capture antibodies was at 500 μg/mL in PBST0.05 （PBS with 0.05% Tween-20）, and the slides were incubated overnight at 4°C. The lowest protein concentration for detection was 2 ng/mL. Each antibody demonstrated high specificity to the corresponding antigen in detecting a mixture of 4 proteins without significant cross-reactivity. The fluorescence and biomarker concentration displayed a linear correlation. The antibody microarray system could be a useful tool for potential biomarker detection for pancreatic cancer.

关键词： cancer biomarker HPCAL1 PEBP1 LgALS7 SERPINE2 antibody microarray

Transcriptome and physiological analyses provide insights into the leaf epicuticular wax accumulation mechanism in yellowhorn

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Horticulture Research 2021年第1期8卷 1851-1863页

作者： Yang zhao Xiaojuan liu Mengke wang Quanxin Bi Yifan Cui libing wang State Key Laboratory of Tree genetics and Breeding Research Institute of ForestryChinese Academy of Forestry100091 BeijingChina State Key Laboratory of Plant genomics Institute of Genetics and Developmental BiologyChinese Academy of Sciences100101 BeijingChina University of chinese academy of sciences 100039 BeijingChina

Plantations and production of yellowhorn,one of the most important woody oil and urban greening trees widely cultivated in northern china,have gradually become limited by drought *** epicuticular wax layer plays a key role in the protection of yellowhorn trees from drought and other ***,there is no research on the mechanism of wax loading in yellowhorn *** this study,we investigated the anatomical and physiological characteristics of leaves from different germplasm resources and different parts of the same tree and compared their cuticle *** addition,the different expression patterns of genes involved in wax accumulation were analyzed,and a coexpression network was built based on transcriptome sequencing *** and physiological comparisons found that the sun leaves from the outer part of the crown had thicker epicuticular wax,which altered the permeability and improved the drought resistance of leaves,than did shade *** on transcriptome data,a total of 3008 and 1324 differentially expressed genes(DEgs)were identified between the sun leaves and shade leaves in glossy-and non-glossy-type germplasm resources,*** identified 138 DEgs involved in wax biosynthesis and transport,including structural genes(such as LACS8,ECH1,and ns-LTP)and transcription factors(such as MYB,WRKY,and bHLH transcription factor family proteins).The coexpression network showed a strong correlation between these *** differences in gene expression patterns between g-and ng-type germplasm resources under different light conditions were very *** results not only provide a theoretical basis for screening and developing drought-resistant yellowhorn germplasm resources but also provide a data platform to reveal the wax accumulation process of yellowhorn leaves.

关键词： properties. yellow drought

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济源冬季VOCs污染特征、来源和SOAP

环境科学 2022年第7期43卷 3451-3462页

作者：王芳李利霞王红果赵宗生张建平张栋河南省济源生态环境监测中心济源459000 郑州大学化学学院郑州450001 郑州大学环境科学研究院郑州450001

重工业城市济源经常发生雾-霾污染事件.挥发性有机化合物(VOCs)是二次有机气溶胶(SOA)生成的前体物,SOA对细颗粒物(PM_(2.5))贡献约15%~20%.于2019年12月1日至12月31日在济源进行PM_(2.5)、O_(3)、VOCs和其他微量气体在线监测,并分析VOC... 详细信息

重工业城市济源经常发生雾-霾污染事件.挥发性有机化合物(VOCs)是二次有机气溶胶(SOA)生成的前体物,SOA对细颗粒物(PM_(2.5))贡献约15%~20%.于2019年12月1日至12月31日在济源进行PM_(2.5)、O_(3)、VOCs和其他微量气体在线监测,并分析VOCs污染特征、来源和SOA生成潜势(SOAP).济源观测到φ(TVOC)平均值为(54.3±27.5)×10^(-9).烷烃、卤代烃和炔烃是主要组分.运用正交矩阵因子分解模型(PMF)识别并分配VOCs来源.确定8个主要VOCs来源:液化石油气/天然气(LPg/ng)、聚氯乙烯(PVC)工业、机动车、焦化工业、溶剂使用、工业、工艺过程和油气挥发.二次有机气溶胶生成潜势分析发现芳香烃对SOAP的贡献最大,其中苯系物(BTEX)对SOAP贡献最大.

关键词：雾-霾济源正交矩阵模型(PMF) 二次有机气溶胶生成潜势(SOAP) 臭氧生成潜势(OFP)

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天师栗叶片DNA提取方法的建立

湖北林业科技 2024年第2期53卷 13-17,56页

作者：吴消潇赵千里王美娟杨思婷廖妮燕江艺蕾杜克兵华中农业大学园艺林学学院武汉430070 神农架林区林业科学研究院神农架442499

提取高质量的基因组DNA是开展分子生物学研究的重要前提之一。天师栗由于叶片含有较多的多糖、多酚类物质,常常导致DNA的提取质量不高,严重影响后续的分子生物学操作。本研究以树龄分别为600、50、4 a的天师栗植株的成熟叶片为材料,建... 详细信息

提取高质量的基因组DNA是开展分子生物学研究的重要前提之一。天师栗由于叶片含有较多的多糖、多酚类物质,常常导致DNA的提取质量不高,严重影响后续的分子生物学操作。本研究以树龄分别为600、50、4 a的天师栗植株的成熟叶片为材料,建立了适合于天师栗叶片DNA的提取方法(M1),并将其与3种常见植物DNA提取方法(M2~M4)进行了比较。结果表明:M1提取DNA的浓度范围介于207.19~488.98 ng·μL^(-1),平均值为308.42 ng·μL^(-1);纯度A_(260)/A_(280)比值介于1.90~1.98,平均值为1.93;A_(260)/A_(230)比值介于1.72~1.95,平均值为1.86;DNA电泳条带清晰,亮度适中,点样孔干净无污染;ISSR-PCR能扩增出条带清晰、稳定性好、多态性高的PCR产物。M1提取出的DNA在浓度、纯度、DNA条带、ISSR-PCR扩增产物方面均明显优于其它3种方法(M2~M4),适合用于天师栗叶片DNA的提取。

关键词：天师栗 DNA提取 DNA质量

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基于国产微滴数字PCR的SNP快速检测技术研究

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生物化学与生物物理进展 2022年第6期49卷 1123-1134页

作者：林培双王寒祝令香贝蕾黄江赵雯婷李彩霞贵州医科大学法医学院贵阳550004 公安部物证鉴定中心法医遗传学公安部重点实验室现场物证溯源技术国家工程实验室北京100038 新羿制造科技有限公司北京100038

目的在法医学领域,现有的SNP检测主要依赖进口,检测工作量大、耗时长且成本较高。微滴数字PCR (droplet digital PCR,ddPCR)作为新一代的PCR技术,可以快速检测低浓度样本DNA,且有较强的抗干扰能力。本研究旨在国产ddPCR平台建立SNP分... 详细信息

目的在法医学领域,现有的SNP检测主要依赖进口,检测工作量大、耗时长且成本较高。微滴数字PCR (droplet digital PCR,ddPCR)作为新一代的PCR技术,可以快速检测低浓度样本DNA,且有较强的抗干扰能力。本研究旨在国产ddPCR平台建立SNP分型检测体系并对其性能进行评估,以探讨ddPCR技术在法医学检验领域的应用价值。方法在ddPCR平台建立高原适应性EPAS1单倍型(rs115321619、rs73926263、rs73926264、rs73926265和rs55981512)检测体系,测试各位点引物探针特异性,对体系的准确性、稳定性、灵敏度、检材适应性进行评估,并比较了ddPCR和SNaPshot微测序检测体系的抗抑制性,最后对样本地区来源进行测试。结果 ddPCR在2.5 h内即可快速获取检测结果,体系准确性和稳定性好,检测灵敏度为0.312 5 ng,且抗抑制性能力突出。70份测试样本检测结果与背景信息一致。结论基于ddPCR的SNP检测体系具有准确可靠、简便快速、抗抑制能力强等优势,在法医学快速检验领域有较强的应用潜力,适合法医现场检验需求。

关键词：微滴数字PCR 法医学检验 SNP分型

Protective effect of nitric oxide on hepatopulmonary syndrome from ischemia-reperfusion injury

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World Journal of gastroenterology 2012年第25期18卷 3310-3316页

作者： Tong-Jin Diao, Xin Chen li-Hua Deng, Han-Xiang ChenI Yan liang Xiao-Dong zhao Qing-Hua wang, Wei-Sheng Yuan Bai-Chun gao Yong Ye Department of Hepatobiliary Surgery and liverTransplant Center Jinan Military Region The Chinese PLA401 st Hospital Qingdao 266071 Shandong Province China Department of Emergency The People's Hospital ofChengyang District Qingdao 266109 Shandong Province ChinaI_i-Hua Deng Central Injection Room Qingdao Municipal Hos-pital Qingdao 266011 Shandong Province China Central Injection Room Qingdao Municipal Hos-pital Qingdao 266011 Shandong Province China institute of-Pathogenic Biology School ofMedicine Shandong University Jinan 250012 Shandong Prov-ince China College of Marine life sciences Ocean Universityof China Qingdao 266003 Shandong Province China Department of general Surgery Jinan MilitaryRegion the Chinese PLA 401st Hospital Qingdao 266071 Shan-dong Province China

AIM： To evaluate immunological protection of nitric ox- ide （NO） in hepatopulmonary syndrome and probable mechanisms of ischemia-reperfusion （IR） injury in rat liver transplantation, METHODS： Sixty-six healthy male Wistar rats were randomly divided into three groups （11 donor/recipi-ent pairs）. In group 11, organ preservation solution was lactated Ringer＇s solution with heparin 10 000/μL at 4℃. In groups I and 111, the pLeservation solution added, respectively, L-arginine or ng-L-arginine methyl ester （L-NAME）（1 mmol/L） based on group 11, and recipients were injected with L-arginine or L-NAME （50 mg/kg） in the anhepatic phase. grafted livers in each group were stored for 6 h and implanted into recipi- ents. Five rats were used for observation of postopera- tive survival in each group. The other six rats in each group were used to obtain tissue samples, and execut- ed at 3 h and 24 h after transplantation. The levels of alanine aminotransferase （ALT）, tumor necrosis factor （TNF）-a and NO metabolites （NOx） were detected, and expression of NO synthase, TNF-α and intercellular adhesion molecule 1 （ICAM-1） was examined by tri- phosphopyridine nucleotide diaphorase histochemical and immunohistochemical staining. RESULTS： By supplementing L-arginine to strengthen the NO pathway, a high survival rate was achieved and hepatic function was improved. One-week sur- viral rate of grafted liver recipients in group I was significantly increased （28.8 4±36.6 d ys 4 4±1.7 d, P 〈 0.01） as compared with groups 11 and Ill. Serum levels of ALT in group ] were 2-7 times less than those in groups 11 and 11I （P 〈 0.01）. The cyclic guanosine monophosphate （cgMP） levels in liver tissue and NOx in group I were 3-4 times higher than those of group 11 after 3 h and 24 h reperfusion, while in group ]]], they were significantly reduced as compared with those in group ]1 （P 〈 0.01）. The levels of TNF-（z in group I were significantly lower than in group Ⅱ after 3 h and 24 h reperfusion （P 〈 0.01）,

关键词： Nitric oxide Nitric oxide synthase Immu-noregulatory Hepatopulmonary syndrome Ischemia-reperfusion injury Orthotopic liver transplantation

gastric cancer cells induce human CD4^+ Foxp3^+ regulatory T cells through the production of TgF-β1

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World Journal of gastroenterology 2011年第15期17卷 2019-2027页

作者： Xiang-liang Yuan Lei Chen Tong-Tong Zhang Yan-Hui Ma Yun-Lan Zhou Yan zhao Wei-Wei wang Ping Dong liang Yu Yan-Yun Zhang li-Song Shen Department of Laboratory Medicine Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai 000 China Department of general Surgery Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200092 China Shanghai institute of Immunology Shanghai Jiao Tong University School of Medicine (SJTUSM) and Institute of Health Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences and SJTUSM Shanghai 200025 China Center for Cancer Research Monash Institute for Medical Research Monash University Melbourne VIC 3168 Australia

AIM: To elucidate the molecular and cellular features responsible for the increase of regulatory T cells (Tregs) in gastric cancer. METHODS: The frequencies of CD4 + Foxp3 + Tregs and the level of transforming growth factor-β1 (TgF-β1) were analyzed from 56 patients with gastric cancer byflow cytometry and enzyme-linked immunosorbent assay respectively. Foxp3.g.ne expression was analyzed by real-time polymerase chain reaction. The gastric cancer microenvironment was modeled by establishing the coculture of gastric cancer cell line, MgC-803, with sorting CD4 + T cells. The normal gastric mucosa cell line, gES-1, was used as the control. The production of TgF-β1 was detected in supernatant of MgC and gES-1. The carboxyfluorescein diacetatesuccinimidyl ester (CFSE) dilution assay was performed to evaluate the proliferation characteristics of induced Tregs. Neutralizing anti-TgF-β1 antibody was added to the co-culture system for neutralization experiments. RESULTS: The level of serum TgF-β1 in gastric cancer patients (15.1 ± 5.5 ng/mL) was significantly higher than that of the genderand age-matched healthy controls (10.3 ± 3.4 ng/mL) (P gher TgF-β1 level correlated with the increased population of CD4 + Foxp3 + Tregs in advanced gastric cancer (r = 0.576, P gnificant higher frequency of CD4 + Foxp3 + Tregs was observed in PBMCs cultured with the supernatant of MgC than gES-1 (10.6% ± 0.6% vs 8.7% ± 0.7%, P ng the purified CD4 + CD25 T cells, we confirmed that the increased Tregs were mainly induced from the conversation of CD4 + CD25 naive T cells, and induced Tregs were functional and able to suppress the proliferation of effector T cells. Finally, we demonstrated that gastric cancer cells induced the increased CD4 + Foxp3 + Tregs via producing TgF-β1. gastric cancer cells upregulated the production of TgF-β1 and blockade of TgF-β1 partly abrogated Tregs phenotype. CONCLUSION: gastric cancer cell can induce

关键词： Transforming growth factor-β1 Regulatory T cells gastric cancer Immune suppression