检索结果-南通市图书馆

lie-Form Invariance of a Type of Non-holonomic Singular Systems

在线全文

学校读者我要写书评

暂无评论

Communications in Theoretical Physics 2007年第3期47卷 409-412页

作者： wang Jing li yuan-Cheng HOU Qi-Bao XIA li-li College of Physical Science and Technology China University of Petroleum Dongying 257061 China

In this paper, the lie-form invariance of a type of non-holonomic singular systems is studied. The differential equations of motion of the systems are given. The definition and the criterions of the lie-form invariance for the systems are presented. The Hojman conserved quantity and the Mei conserved quantity are obtained. An example is given to illustrate the application of the results.

关键词： non-holonomic constraint singular system symmetry conserved quantity

Form Invariance and Conserved Quantity for Non-holonomic Systems with Variable Mass and Unilateral Constraints

在线全文

学校读者我要写书评

暂无评论

Communications in Theoretical Physics 2007年第2期47卷 213-216页

作者： wang Jing li yuan-Cheng HOU Qi-Bao XIA li-li College of Physical Science and Technology University of Petroleum China Dongying 257061 China

The paper studies the form invariance and a type of non-Noether conserved quantity called Mei conserved quantity for non-holonomic systems with variable mass and unilateral constraints. Acoording to the invariance of the form of differential equations of motion under infinitesimal transformations, this paper gives the definition and criterion of the form invariance for non-holonomic systems with variable mass and unilateral constraints. The condition under which a form invariance can lead to Mei conservation quantity and the form of the conservation quantity are deduced. An example is given to illustrate the application of the results.

关键词： variable mass unilateral constraint non-holonomic system form invaxiance conserved quantity

Protein-protein Interaction Between Domains of PDZ and BAR from PICK1

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2007年第2期23卷 191-195页

作者： XIAO Hong SHI Ya-wei wang li-li yuan Jing-ming Institute of Biotechnology Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education Shanxi University Taiyuan 030006 P. R. China

Two DNA fragments encoding PDZ domain （21-110 residues） and BAR domain （ 150-360 residues） from PICK1 （1-416 residues） were amplified by PCR and then introduced into vectors, pET-32M and pMAL-e2X respectively to generate recombinant plasmids, pE-pdz and pM-bar. Having been separately transferred into the hosts E. coli BL21 and E. coli JM109, these two strains can express fusion proteins： His-tagged PDZ（PDZ domain） and maltose binding protein-BAR（ MBP-BAR domain） respectively, as confirmed by both SDS-PAGE and Wostem blotting. The interaction between these two domains is dose-dependence, as identified by a pull-down test. Moreover, it has been shown from the EliSA analysis that the actual amount of PDZ bound to MBP-BAR-amylose beads reaches （ 16 ± 0. 5）%, as calculated by the molar ratio of PDZ to MBP-BAR. In addition, the interaction between BAR（bait） and PDZ（prey） in vivo was also examined with a yeast two-hybrid system.

关键词： BAR domain PDZ domain PICK1 Protein-protein interaction Pull-down test Yeast two-hybrid

Copper(Ⅰ)-catalyzed Synthesis of Aryl, Heteroaryl and Vinyl Sulfides in Aqueous Two-phase System

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2011年第3期27卷 417-421页

作者： wang li-jin yuan Yan-qin GUO Sheng-rong College of Chemistry and life Science Lishui University Lishui 323000 P. R. China

A fast and convenient C―S bond formation reaction was achieved catalyzed by CuI/L-proline in aqueous two-phase system providing a simple method for synthesis of aryl sulfides in good yields in short time.

关键词： C―S cross-coupling reaction CuI Thiol Aryl halide Aqueous two-phase system

Endogenous nitric oxide and hydrogen peroxide detection in indole-3-butyric acid-induced adventitious root formation in Camellia sinensis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第10期17卷 2273-2280页

作者： WEI Kang wang li-yuan RUAN li ZHANG Cheng-cai WU li-yun li Hai-lin CHENG Hao Key Laboratory of Tea Biology and Resources Utilization Ministry of Agriculture National Center for Tea Improvement Tea Research Institute Chinese Academy of Agricultural Sciences(TRICAAS)

Nitric oxide（NO）and hydrogen peroxide（H2O2）are essential signaling molecules with key roles in auxin induced adventitious root formation in many ***,whether they are the sole determinants for adventitious root formation is worth further *** this study,endogenous NO and H2O2 were monitored in tea cutting with or without indole-3-butyric acid（IBA）treatment by using the fluorescent probes diaminofluorescein diacetate（DAF-2DA）and 2＇,7＇-dichlorodihydrofluorescein diacetate（DCF-DA）,*** overproduction of NO and H2O2 was detected in the rooting parts of tea cuttings treated with or without *** little NO and H2O2 was detected before the initiation phase of tea cuttings even with IBA treatment indicating that they might be not directly induced by *** carbon and nitrogen analysis found that the overproduction of NO and H2O2 were coincident with the consumption of soluble sugars and the assimilation of *** results suggest that rooting phases should be taken into consideration with the hypothesis that auxin induces adventitious root formation via NO-and H2O2-dependent pathways and sink establishment might be a prerequisite for NO and H2O2 mediated adventitious root formation.

关键词： nitric oxide hydrogen peroxide indole-3-butyric acid tea cuttings

Synthesis and Pharmacological Properties of 5-Alkyl Substituted Nicotine Analogs

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2012年第12期30卷 2813-2818页

作者： wang Jing li Xi yuan Qianjia Ren Jiangmeng Huang Jin Zeng Bubing Shanghai Key Laboratory of Chemical Biology School of Pharmacy East China University of Science and Technology Shanghai 200237 China

This paper describes a concise and practical route to enantiomerically enriched 5-alkyl substituted nicotine analogs. The Vilsmeier reaction was used to construct the nicotinaldehydes ring followed by the introduction of the chiral homoallylic alcohol by organic boron reagent and the cyclization of the pyrrolidine ring through the reduction of a chiral azide. 17 analogs have been synthesized and their corresponding biological activities were tested, in which compounds 10d and 10g exhibit excellent ICs0 values against RD and SY-SY5Y.

关键词： nicotine analog asymmetric synthesis Vilsmeier reaction biological activities

MUC1-positive circulating tumor cells and MUC1 protein predict chemotherapeutic efficacy in the treatment of metastatic breast cancer

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer 2011年第1期30卷 54-61页

作者： Jian-Ping Cheng Ying Yan Xiang-Yi wang yuan-li Lu Yan-Hua yuan Jun Jia Jun Ren Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Peking University School of Oncology Department of Medical Oncology Beijing Cancer Hospital & Institute Beijing 100142 P. R. China.

Chemotherapy plays an important role in the treatment of metastatic breast cancer. It is important to monitor chemotherapeutic efficacy, to find a simple and efficient tool to guide treatment, and to predict the efficacy of treatment in a timely and accurate manner. This study aimed to detect mucin-1 (MUC1) - positive circulating tumor cells and MUC1 protein in the peripheral blood of patients with metastatic breast cancer and to investigate their relationship to chemotherapeutic efficacy. MUC1 mRNA was detected in the peripheral blood of 34 patients with newly diagnosed metastatic breast cancer by reverse transcription- polymerase chain reaction. The positive rates of MUC1 mRNA were 88.2% before chemotherapy and 70.6% after chemotherapy, without a significant difference (P = 0.564); MUC1 mRNA expression before chemotherapy had no correlation with treatment effectiveness (P = 0.281). The response rate of MUC1 mRNA -negative patients after first-cycle chemotherapy was significantly higher (P = 0.009) and the progression-free survival (PFS) was clearly longer than those of MUC1 mRNA-positive patients (P = 0.095). MUC1 protein in peripheral blood plasma was detected by an EliSA competitive inhibition assay. The patients with decreased MUC1 protein after chemotherapy had a significantly longer PFS than those with elevated MUC1 protein (P = 0.044). These results indicate that the outcomes of MUC1 mRNA - negative patients after chemotherapy are better than those of MUC1 mRNA-positive patients. In addition, patients with decreased expression of MUC1 protein have a better PFS.

关键词： MUC1 治疗效果肿瘤细胞阳性率乳腺癌转移性粘蛋白化疗

Expression of gastric cancer-associated MG7 antigen in gastric cancer,precancerous lesions and ***-associated gastric diseases

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2002年第6期8卷 1009-1013页

作者： Dong-li Guo Ming Dong Lan wang li-Ping Sun yuan yuan,Cancer Institute,First Affiliated Hospital,China Medical University,Shenyang,110001,liaoning Province,China Cancer Institute First Affiliated Hospital China Medical University Shenyang 110001 Liaoning Province China.

AIM: To investigate the relationship between the antigen MG7 antigen expression and gastric cancer as well as precancerous condition; to study the relationship between the MG7 antigen expression and H. pyloriinfection in benign gastric lesions in order to find out the effect of H. pylori infection on the process of gastric cancer ***: The level of MG7 antigen expression was determined by immunohistochemical method in 383 gastric biopsied materials. The intestinal metaplasia was determined by histochemistry method. The H. pyloriinfection was determined by HE stain, PCR and EliSA in 291 specimens, among which only 34 cases of H. pylori-associated gastric lesions were followed ***: The positive rate of MG7 expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric cancer increased gradually in ascending order (P＜0.01). The positive rate of MG7 antigen expression in type Ⅲ intestinal metaplasia of gastric mucosa was higher than that of type Ⅰand Ⅱ intestinal metaplasia, being highly significant (P＜0.05).The positive rate of MG7 antigen expression in superficial gastritis, atrophic gastritis and gastric cancer increased gradually (11.9 %, 64.8 %, 91.2 %, P＜0.01). There was no significant difference between ***-negative and H. pyloripositive intestinal metaplasia, atrophic gastritis and dysplasia of gastric epithelium in the positive rate of MG7 antigen expression. There was no expression of MG7 antigen in H. pylori-negative superficial gastritis. The positive rate of MG7 expression in H. pylori-positive superficial gastritis was 20.5 %, and the difference between them was significant (P＜0.05). During following up, one of the three H. pylori negative cases turned positive again, and its MG7 antigen expression turned to be stronger correspondingly. 3 of 31 H. pyloripositive cases were detected as early gastric cancer, among which one with '+++' MG7 antigen expression was diminished after H. pylori ***: M

关键词：胃癌 MG7抗原基因表达癌前病变幽门螺杆菌相关胃疾病

Optimization of Three-Dimensional Culture Conditions of HepG2 Cells with Response Surface Methodology Based on the VitroGel System

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第8期35卷 688-698页

作者： wang Jing Bo QIN Wen YANG Zhuo SHEN Shi MA Yan wang li yuan ZHUO Qin GONG Zhao Long HUO Jun Sheng CHEN Chen National Institute for Nutrition and Health Chinese Centre for Disease Control and PreventionBeijing 100050China

Objective This study optimizes three-dimensional(3D) culture conditions of HepG2 using response surface methodology(RSM) based on the VitroGel system to facilitate the cell model in vitro for liver *** HepG2 cell was 3D cultured on the VitroGel *** viability was detected using Cell Counting Kit-8(CCK-8) assay of HepG2 lived cell *** proliferation of HepG2 cell and clustering performance was measured via fluorescence staining *** concentration in the culture medium supernatant as an index of HepG2 cell biological function was measured with EliSA *** factor tests were conducted with three key factors:inoculated cell concentration,cultured time,and dilution degree of the *** preliminary results of independent factor tests were used to determine the levels of factors for *** The selected optimal culture conditions are as follows:concentration of inoculated cells was4.44 × 10^(5)/mL,culture time was 4.86 days,and hydrogel dilution degree was 1:*** result shows that under optimal conditions,the predicted optical density(OD) value of cell viability was 3.10 and measured 2.978 with a relative error of 3.94%.Conclusion This study serves as a reference for the 3D HepG2 culture and constructs liver tissues in ***,it provides the foundation for repeated dose high-throughput toxicity studies and other scientific research work.

关键词： Response surface methodology The three-dimensional culture Hydrogel