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Tissue Culture System for Different Hybrid of Indica Rice

Journal of Northeast Agricultural University(English Edition) 2011年第2期18卷 13-17页

作者： wang li lin gang zhao deming wang feng chen jiabin Institute of Rice Research Yibin Academy of Agricultural Sciences Yibin 644000 Sichuan China

The effect of varieties and media compositions on callus induction from rice anther and subsequent plant regeneration was studied. The results showed that the callus induction was not significantly different in different media, but mostly depended on genotypes; mean frequency of callus induction on F1 hybrid varieties showed that the medium supplemented with 100 mg·L^-1 concentration of the proline was useful for callus induction; when the anther derived callus sub-cultured on MS medium supplemented with BAP （0.5 mg·L^-1）, KT （1.0 mg·L^-1）, IAA （0.25mg·L^-1） and NAA （0.5 mg·L^-1） for plant regeneration, the frequency of regeneration ranged from 0 to 54.17%, and the highest differentiation index reached 30.5 after sub-cultured for three times; the plants were rooted after transferred to 1/2MS medium with NAA （0.5 mg·L^-1） and IAA （0.5mg·L^-1）.

关键词： anther culture callus induction regeneration sub-cultured

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国审宜香系列杂交稻特征特性分析

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种子 2019年第6期38卷 135-138页

作者：陈家彬林纲赵德明江青山贺兵姜方洪韩冬王丽宜宾市农业科学院

水稻是我国重要粮食作物,而杂交水稻的发现和利用,有效增加了我国粮食产量,保证了我国的粮食安全。宜香系列杂交稻是以水稻三系不育系宜香1 A为母本并通过审定的三系杂交稻组合,本文以国家水稻数据中心通过国审的16个宜香系列杂交稻的... 详细信息

水稻是我国重要粮食作物,而杂交水稻的发现和利用,有效增加了我国粮食产量,保证了我国的粮食安全。宜香系列杂交稻是以水稻三系不育系宜香1 A为母本并通过审定的三系杂交稻组合,本文以国家水稻数据中心通过国审的16个宜香系列杂交稻的各项性状数据为材料,对国审宜香系列杂交稻的特性性状、产量、抗性和米质进行了分析。

关键词：宜香杂交稻分析

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A draft sequence of the rice (Oryza sativa ssp. indica) genome

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Chinese Science Bulletin 2001年第23期46卷 1937-1942页

作者： YU Jun, HU Songnian, wang Jun,li Songgang WONG Ka-Shu Gane, liU Bin,DENG Yajun, DAI li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, liU Jing,SUN Jiandong , TANG jiabin, chen Yanjiong,HUANG Xiaobing, lin Wei, YE chen, TONG Wei,CONG lijuan, GENG Jianing, HAN Yujun, li lin,li Wei, HU Guangqiang, HUANG Xiangang,li Wenjie, li Jian, liU Zhanwei, li Long,liU Jianping, Ql Qiuhui, liU Jinsong, li li,wang Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, feng Xiaoli, GUI Peng,li Xianran, wang Hao, XU Xin, ZHAI Wenxue,XU zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO lin, chen Xuewei, HE Jun, liU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,li gang, GAO Hui, li Ping, chen Wei ,wang Xudong, ZHANG Yong, HU Jianfei,wang Jing, liU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, li Zhijie,MAO Long, ZHOU chengshu, ZHU Zhen,chen Runsheng, HAO Bailin,ZHENG Weimou, chen Shouyi, QUO Wei,li Guojie, liU Siqi, HUANG Guyang,TAO Ming, wang Jian, ZHU lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden Beijing Genomics Institute/Center of Genomics & Bioinformatics Chinese Academy of Sciences Beijing China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province Hangzhou China College of life Sciences Peking University Beijing China Medical College Xi’an Jiaotong University Xi’an China Institute of Genetics Chinese Academy of Sciences Beijing China Laboratory of Bioinformatics Institute of Biophysics Chinese Academy of Sciences Beijing China National Hybrid Rice R & D Center Changsha China Institute of Theoretical Physics Chinese Academy of Sciences Beijing China Digital China Ltd. Beijing China Institute of Computing Technology Chinese Academy of Sciences Beijing China

The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package

关键词： rice genome draft sequences.

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