检索结果-南通市图书馆

A bivalent recombinant vaccine:a promising strategy against both SARS-CoV-2 variants and wild type of the virus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第8期6卷 2266-2267页

作者： junzhi wang Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products National Institutes for Food and Drug ControlBeijingChina

A recent study published in Medcomm by He et al.1 introduced protein subunit vaccines with wild-type and mutant S1 subunit(S1-WT and S1-Mut)of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)to evaluate their immunoprotective *** team further analyzed the neutralization activity of bivalent vaccine composed of S1-WT and S1-Mut for SARS-CoV-2 and mutant strains to explore a universal protective vaccine.

关键词： protective vaccine

CO observation of SNR IC 443

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2010年第7期53卷 1357-1369页

作者： ZHANG ZhiYu GAO Yu wang junzhi Purple Mountain Observatory Chinese Academy of SciencesNanjing 210008China Graduate University of Chinese Academy of Sciences Beijing 100080China Astronomy Department Nanjing UniversityNanjing 210093China

We present our 12CO and 13CO mapping observations of SNR IC 443 interacting with molecular *** is the first largescale high-resolution 13CO mapping observation in the surrounding *** morphologies of IC 443 in 12CO and 13 CO are compared with the optical,infrared,Spitzer far-infrared,X-ray,and neutral atomic gas(HI).We also make comparison and analysis in the kinematics,using the date-cubes of 12CO,13CO and HI,to help distinguish the complicated gas motions in the shocked *** on the work of wang & Scoville(1992),we present a new model to explain the coexistence of multiple shocks with different speeds in a pc-scale region at the central clump *** test this new model by analyzing the HI and CO distribution in both velocity and space *** also establish the relationship between the dissociation rate of shocked molecular gas and the shock velocity in this ***,we derive the optical depth of 12CO with the 13CO spectra in clump B,and discuss the validity of the assumption of optically thin emission for the shocked 12CO.

关键词： supernova remnant shock molecular clouds molecular spectrum IC 443

Review on Drug Regulatory Science Promoting COVID-19 Vaccine Development in China

在线全文

学校读者我要写书评

暂无评论

Engineering 2022年第3期8卷 127-132页

作者： Zhiming Huang Zhihao Fu junzhi wang National Medical Products Administration Beijing 100053China National Institutes for Food and Drug Control Beijing 102629China

Regulatory science is a discipline that uses comprehensive methods of natural science,social science,and humanities to provide support for administrative decision-making through the development of new tools,standards,and approaches to assess the safety,efficacy,quality,and performance of regulated *** the pandemics induced by infectious diseases,such as H1N1 flu,severe acute respiratory syndrome(SARS),and Middle East respiratory syndrome(MERS),regulatory science strongly supported the development of drugs and vaccines to respond to the *** particular,with the support of research on drug regulatory science,vaccines have played a major role in the prevention and control of coronavirus disease 2019(COVID-19).This review summarizes the overall state of the vaccine industry,research and development(R&D)of COVID-19 vaccines in China,and the general state of regulatory science and supervision for vaccines in ***,this review highlights how regulatory science has promoted the R&D of Chinese COVID-19 vaccines,with analyses from the aspects of nationallevel planning,relevant laws and regulations,technical guidelines,quality control platforms,and postmarketing ***,this review provides a reference for the formulation of a vaccine development strategy in response to the current pandemic and the field of vaccine development in the post-pandemic era,as well as guidance on how to better respond to emerging and recurring infectious diseases that may occur in the future.

关键词： Regulatory science COVID-19 vaccine Vaccine industry

Optimized functional and structural design of dual-target LMRAP,a bifunctional fusion protein with a 25-amino-acid antitumor peptide and GnRH Fc fragment

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2020年第2期10卷 262-275页

作者： Meng Li Hanmei Xu junzhi wang Shenyang Pharmaceutical University Shenyang 110016China Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products National Institutes for Food and Drug ControlBeijing 102629China State Key Laboratory of Natural Medicines Ministry of Educationthe Engineering Research Center of Synthetic Polypeptide Drug Discovery and Evaluation of Jiangsu ProvinceDepartment of Marine PharmacyChina Pharmaceutical UniversityNanjing 211198China

To develop fusion protein of a GnRH Fc fragment and the integrin targeting AP25 antitumor peptide for GnRH receptor-expressing cancer *** LMRAP fusion protein was constructed.A transwell invasion assay was *** gene mRNA and protein levels of GnRHR-I,α5β1,andαvβ3 in different cancer cell lines were *** proliferation was measured using a cell counting *** antagonist assay was performed on GnRH ***-tumor activity was evaluated with a mouse xenograft tumor ***(IHC)was applied to detect CD31 and CD34 *** characteristics were determined with an indirect competition *** developed bifunctional fusion protein LMRAP not only inhibited HUVEC invasion,but also inhibited proliferation of GnRHR-I,α5β1,and high expression cancer *** IC50 for LMRAP in the GnRH receptor was 6.235 x 10-4 mol/*** significantly inhibited human prostate cancer cell line22 RV1 proliferation in vivo and in *** significantly inhibited CD31 and CD34 *** elimination half-life of the fusion protein LMRAP was 33 h in *** fusion protein made of a GnRH Fc fragment and the integrin targeting AP25 peptide retained the bifunctional biological activity of GnRHR blocking,angiogenesis inhibition,prolonged half-life and good tolerance.

关键词： Fusion protein GnRH Integrin Angiogenesis Prostate cancer

Density Functional Theory Calculations on the Interstellar Formation of Biomolecules

在线全文

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2023年第12期23卷 7-18页

作者： Qingli Liao junzhi wang Peng Xie Enwei Liang Zhao wang Laboratory for Relativistic Astrophysics Department of PhysicsGuangxi UniversityNanning 530004China School of Chemistry and Chemical Engineering Guangxi UniversityNanning 530004China

Density functional theory(DFT)is the most versatile electronic structure method used in quantum chemical calculations,and is increasingly applied in astrochemical *** mini-review provides an overview of the applications of DFT calculations in understanding the chemistry that occurs in star-forming *** survey investigations into the formation of biologically relevant compounds such as nucleobases in the interstellar medium,and also cover the formation of both achiral and chiral amino acids,as well as biologically relevant molecules such as sugars,and nitrogen-containing polycyclic aromatic ***,DFT calculations are used to estimate the potential barriers for chemical reactions in astronomical *** conclude by noting several areas that require more research,such as the formation pathways of chiral amino acids,complex sugars,and other biologically important molecules,and the role of environmental factors in the formation of interstellar biomolecules.

关键词： astrochemistry-molecular processes-methods laboratory molecular-astrobiology

Quality Control and Nonclinical Research on CAR-T Cell Products: General Principles and Key Issues

在线全文

学校读者我要写书评

暂无评论

Engineering 2019年第1期5卷 122-131页

作者： Yonghong Li Yan Huo Lei Yu junzhi wang National Institutes for Food and Drug Control Beijing 100050 China

Adoptive cell therapy using chimeric antigen receptor T (CAR-T) cells, which is a promising cancer immunotherapy strategy, has been developing very rapidly in recent years. CAR-T cells are genetically modified T cells that can specifically recognize tumor specific antigens on the surface of tumor cells, and then effectively kill tumor cells. At present, exciting results are being achieved in clinical applications of CAR-T cells for patients with hematological malignancies. The research and development of CAR-T cells for various targets and for the treatment of solid tumors have become a hot topic worldwide, so an increasing number of investigational new drug applications (INDAs) and new drug applications (NDAs) of CAR-T cell products are expected to be submitted in future. The quality control and nonclinical research of these products are of great significance in ensuring the safety and effectiveness of these products;however, they also present great challenges and difficulties. This article discusses the general principles of and key issues regarding the quality control and nonclinical research of CAR-T cell products based on their product characteristics and on relevant guidelines for gene and cell therapy products.

关键词： Chimeric antigen receptor T cells Quality control Nonclinical research Safety Efficacy Clinical trials Cancer immunotherapy

A reporter gene assay for determining the biological activity of therapeutic antibodies targeting TIGIT

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第12期11卷 3925-3934页

作者： Zhihao Fu Hongchuan Liu Lan wang Chuanfei Yu Yalan Yang Meiqing Feng junzhi wang Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products National Institutes for Food and Drug ControlBeijing 102629China Department of Biological Medicines&Shanghai Engineering Research Center of Immunotherapeutics School of PharmacyFudan UniversityShanghai 201203China

T cell immunoglobulin and ITIM domain(TIGIT)is a novel immune checkpoint that has been considered as a target in cancer *** available bioassays for measuring the biological activity of therapeutic antibodies targeting TIGIT are restricted to mechanistic investigations because donor primary T cells are highly ***,we designed a reporter gene assay comprising two cell lines,namely,CHO-CD112-CD3 scFv,which stably expresses CD 112(PVRL2,nectin-2)and a membranebound anti-CD3 single-chain fragment variable(scFv)as the target cell,and Jurkat-NFAT-TIGIT,which stably expresses TIGIT as well as the nuclear factor of activated T-cells(NFAT)response element-controlled luciferase gene,as the effector *** anti-CD3 scFv situated on the target cells activates Jurkat-NFATTIGIT cells through binding and crosslinking CD3 molecules of the effector cell,whereas interactions between CD 112 and TIGIT prevent *** presence of anti-TIGIT mAbs disrupts their interaction,which in turn reverses the inactivation and luciferase *** and validation studies have demonstrated that this assay is superior in terms of specificity,accuracy,linearity,and *** summary,this reliable and effective reporter gene assay may potentially be utilized in lot release control,stability assays,screening,and development of novel TIGIT-targeted therapeutic antibodies.

关键词： TIGIT Therapeutic antibodies Bioactivity determination Reporter gene assay Method validation

A rapid reporter assay for recombinant human brain natriuretic peptide(rhBNP)by GloSensor technology

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2018年第5期8卷 297-301页

作者： Lei Yu Xinchang Shi Chunmei Han Chunming Rao junzhi wang National Institutes for Food and Drug Control Beijing 100050China WHO CoUahoration Centre for Biologicals Standardization and Evaluation Beijing 100050China

Accurate determination of biological activity is essential in quality control of recombinant human brain natriuretic peptide （rhBNP）. In previous study, we successfully developed a genetically modified cell line 293GCAC3-based ELISA assay for rhBNP. But ELISA procedure is still tedious, so this study was aimed to develop a rapid and simple bioassay for rhBNP using GloSensor technology, which provides a platform of flexible luciferase-based biosensors for real-time detection of signaling events in live cells, including cGMP production. A reporter cell line 293GCAGIo-G1 was constructed by transfecting pGloSensorTM 40 F plasmid into 293GCAC3. The reporter assay based on 293GCAGIo-G1 showed high precision with intraassay CV being 8.3% and inter-assay CV being 14.1%; high accuracy with 80%, 100% and 120% recovery rate being 99.2%, 102.4% and 99.0% respectively; and great linearity with R^2 of linear fitting equation being 0.99. Besides, no significant difference was found in test results of reporter assay and 293GCAC3-based ELISA assay （paired t test,p = 0.630）. All these results suggested that the reporter assay was a viable assay for biological determination of rhBNP.

关键词： RhBNP cGMP GloSensor technology Reporter assay

Synthesis, Structure and H＋/K＋-ATPase Inhibitory Activity of Novel Triazolyl Substituted Tetrahydrobenzofuran Derivatives via One-pot Three-component Click Reaction

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2013年第6期31卷 831-838页

作者： Hubiao Fang Lei Jin Nianyu Huang junzhi wang Kun Zou Zaigang Luo Hubei Key Laboratory of Natural Products Research and Development College of Chemistry and Life Sciences China Three Gorges University Yichang Hubei 443002 China Hubei Tujia Institute of Medicine China Three Gorges University Yichang Hubei 443002 China College of Chemical Engineering Anhui University of Science & Technology Huainan Anhui 232001 China

Eleven triazolyl substituted tetrahydrobenzofuran derivatives were synthesized in high yields as novel H＋/K＋- ATPase inhibitor via one-pot Cul-catalyzed three-component click reaction of azide, secondary amine and 3-bromopropyne under mild conditions in water. Their structures were characterized by NMR, IR, ESI-MS, ele- mental analysis and single-crystal X-ray diffraction analysis. Most of the target compounds exhibited better H＋/K＋- ATPase inhibitory activity than commercial omeprazole with IC50 values less than 15 gmol＇L-~. The initial struc- ture-activity analysis suggested that the triazole substituted by cycloalkyl, aromatic ring or O-containing side-chain seemed to be beneficial for enhancing the activity.

关键词： tetrahydrobenzofuran three-component click reaction H+/K+-ATPase inhibitor X-ray diffractionanalysis synthesis