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穿心莲内酯生物合成与调控的研究进展

广东农业科学 2024年第5期51卷 69-80页

作者：张龙顾艳李静宇徐世强孙铭阳胡学博王继华华中农业大学植物科学技术学院湖北武汉430070 广东省农作物遗传改良重点实验室/广东省农业科学院作物研究所广东广州510640

穿心莲内酯是中药材穿心莲的主要活性成分,具有清热解毒、抗菌消炎、保肝利胆等功效,临床上主要用于治疗小儿肺炎和上呼吸道感染等疾病。近年来,由于环境因素长期影响、品种纯度不高以及优良品种选育工作滞后等原因,很大程度上限制了穿... 详细信息

穿心莲内酯是中药材穿心莲的主要活性成分,具有清热解毒、抗菌消炎、保肝利胆等功效,临床上主要用于治疗小儿肺炎和上呼吸道感染等疾病。近年来,由于环境因素长期影响、品种纯度不高以及优良品种选育工作滞后等原因,很大程度上限制了穿心莲的品质提升。在穿心莲药材质量评价中,穿心莲内酯的含量是一项非常重要的指标,通过解析穿心莲内酯生物合成的分子机制及积累规律,有助于提升穿心莲药材质量。该文综述了穿心莲内酯的生物合成途径与分子调控机制,穿心莲内酯的生物合成主要分为两大阶段,即穿心莲内酯前体的合成阶段和穿心莲内酯合成的修饰阶段,其中前体合成研究已经较为清楚,但有关穿心莲内酯合成修饰阶段的途径和关键酶的挖掘还处于空白水平,需进一步研究。通过从环境因素和栽培措施两个层面对穿心莲内酯合成积累的关键影响因子进行挖掘,有利于建立完善的高品质穿心莲种植体系。此外,对调控穿心莲内酯生物合成的研究进展进行总结和展望,可为穿心莲药材品质提升提供参考。

关键词：穿心莲穿心莲内酯生物合成调控环境因素栽培措施

植皮术后施予环形加压包扎或负压封闭引流的研究

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云南医药 2024年第5期45卷 20-23页

作者：傅听雨王继华何永静朱礼昆杨蔚琪徐路娟昆明医科大学第二附属医院整形外科云南昆明650101

目的探讨四肢皮肤缺损植皮术后分别应用环形加压包扎与VSD的临床效果。方法选取2016年9月-2021年9月昆明医科大学第二附属医院整形外科收治的四肢皮肤缺损患者103例,按照植皮术后固定方式不同分为环形加压包扎组56例和VSD组47例,对比分... 详细信息

目的探讨四肢皮肤缺损植皮术后分别应用环形加压包扎与VSD的临床效果。方法选取2016年9月-2021年9月昆明医科大学第二附属医院整形外科收治的四肢皮肤缺损患者103例,按照植皮术后固定方式不同分为环形加压包扎组56例和VSD组47例,对比分析2组皮片存活率、并发症发生率、住院时间及植皮区周围正常皮肤不良反应发生率。结果在皮片存活率、住院时间、并发症发生率上2组相比差异无统计学意义(P>0.05),环形加压包扎组患者植皮区周围正常皮肤不良反应发生率低于VSD组差异有统计学意义(P<0.05)。结论四肢植皮术后采用环形加压包扎方式对患者固定,患者植皮区周围正常皮肤较少发生不良反应。

关键词：加压包扎负压封闭引流皮肤缺损植皮术

Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis

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Science Bulletin 2024年第13期69卷 2099-2113页

作者： Jianfei Liang Jing wang Bingdong Sui Yibo Tong jihua Chai Qin Zhou Chenxi Zheng Hao wang Liang Kong Haojian Zhang Yi Bai State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration Key Laboratory of Oral Biomedicine Ministry of EducationHubei Key Laboratory of StomatologySchool&Hospital of StomatologyTaikang Center for Life and Medical SciencesWuhan UniversityWuhan 430079China Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi’an Jiaotong UniversityXi’an 710004China Department of Implant Dentistry College of StomatologyXi’an Jiaotong UniversityXi’an 710004China State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration National Clinical Research Center for Oral DiseasesShaanxi Key Laboratory of StomatologyShaanxi International Joint Research Center for Oral DiseasesCenter for Tissue EngineeringSchool of StomatologyThe Fourth Military Medical UniversityXi’an 710032China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration National Clinical Research Center for Oral DiseasesShaanxi Key Laboratory of StomatologyDepartment of Oral and Maxillofacial SurgerySchool of StomatologyThe Fourth Military Medical UniversityXi’an 710032China Department of Hematology Zhongnan HospitalWuhan UniversityWuhan 430079China Frontier Science Center for Immunology and Metabolism Medical Research InstituteWuhan UniversityWuhan 430079China Taikang Center for Life and Medical Sciences Wuhan UniversityWuhan 430079China

Stem cells remain in a quiescent state for long-term maintenance and preservation of potency;this process requires fine-tuning regulatory *** this study,we identified the epigenetic landscape along the developmental trajectory of skeletal stem cells(SSCs)in skeletogenesis governed by a key regulator,Ptip(also known as Paxip1,Pax interaction with transcription-activation domain protein-1).Our results showed that Ptip is required for maintaining the quiescence and potency of SSCs,and loss of Ptip in type II collagen(Col2)^(+)progenitors causes abnormal activation and differentiation of SSCs,impaired growth plate morphogenesis,and long bone *** also found that Ptip suppressed the glycolysis of SSCs through downregulation of phosphoglycerate kinase 1(Pgk1)by repressing histone H3 lysine 27 acetylation(H3K27ac)at the promoter ***,inhibition of glycolysis improved the function of SSCs despite Ptip *** the best of our knowledge,this is the first study to establish an epigenetic framework based on Ptip,which safeguards skeletal stem cell quiescence and potency through metabolic *** framework is expected to improve SSC-based treatments of bone developmental disorders.

关键词： Skeletal stem cells Bone development Epigenetics Ptip Glycolysis

Forecasting S&P 500 Stock Index Using Statistical Learning Models

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Open Journal of Statistics 2016年第6期6卷 1067-1075页

作者： Chongda Liu jihua wang Di Xiao Qi Liang Department of Industrial Engineering University of Illinois at Urbana-Champaign Urbana IL USA

Forecasting the movement of stock market is a long-time attractive topic. This paper implements different statistical learning models to predict the movement of S&P 500 index. The S&P 500 index is influenced by other important financial indexes across the world such as commodity price and financial technical indicators. This paper systematically investigated four supervised learning models, including Logistic Regression, Gaussian Discriminant Analysis (GDA), Naive Bayes and Support Vector Machine (SVM) in the forecast of S&P 500 index. After several experiments of optimization in features and models, especially the SVM kernel selection and feature selection for different models, this paper concludes that a SVM model with a Radial Basis Function (RBF) kernel can achieve an accuracy rate of 62.51% for the future market trend of the S&P 500 index.

关键词： Statistical Learning Models S&P 500 Index Feature Selection SVM RBF Kernel

Effect of Different Pesticides on Lipase Production by Isaria fumosorosea

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Agricultural Science & Technology 2017年第12期18卷 2503-2506,2523页

作者： zeqing wang jihua wang zhenfang shao sujuan yan guangzhou harit bioscience co. ltdGuangzhou 510507China guangdong engineering research center of microbe pesticides guangdong newscene biological engineering co.ltdYangjiang 529932China crop research institute guangdong academy of agriculture sciencesGuangzhou 510640China conghua entry-exit inspection and quarantine bureau Guangzhou 510900China

The influence of twelve different pesticides(six insecticides and six fungicides) on lipase production by Isaria fumosorosea was investigated under laboratory conditions. Imidacloprid caused higher reduction in enzyme activities at all three concentrations whereas very low reduction in enzyme activities was caused by azadrichtin and Indoxacarb when used at 10μg/ml. In case of fungicides, propiconazole and pyraclostrobin caused higher reduction in enzyme activities at all three concentrations whereas low reduction in enzyme activities was caused by chlorothalonil and mancozeb when used at 10 μg/ml. Different insecticides significantly affected the toxicity of I. fumosorosea lipase against second instar Dysmicoccus neobrevipesnymphs. *** lipase combined with azadrichtin was proved to be the most aggressive, and those from control were the least virulent with a mean ST50 value of 6.34 ± 0.41 d.

关键词： Isaria fumosorosea Lipase production Insecticides Fungicides

Iteration Linear Programming Method and Livestock Ration Optimization

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Feed & Livestock 2013年第4期6卷 26-32页

作者： Zhang Yongying wang jihua wang Suihua Liu Bo Zhang Weifeng Department Hebei University of Engineering Handan~ 056002. P.R.China.

Iterative linear programming methods are proposed for optimum balanced animal diet in this paper. According to ＂wooden bucket theory＂ of the nutritional balance, each nutrient in the feeding standard has equal importance. It＇s unreasonable to use common goal programming to attach different weighted value to different nutritional parameters. This paper introduces an effective algorithm to deal with this kind of problem. When the permitting cost of livestock ration is given, we can design a ration formula with linear program-this is the first round. Then, according to the differences between the permitting cost and the formula cost gained in the first round, adjust the feeding standard and the feeding raw materials, and conduct the second round of linear programming for ration formula. If there is still a very big difference between the formula cost and the permitting cost, the third round will be taken, and so on. In this iteration course the formula cost gradually approaches the permitting cost. It is the key that the feeding standard and feeding raw materials are modified in each round. This method ensured the nutritive equilibrium with the formulation of least-cost ration. This is an especially important method when the primary goal of the optimization tool is to improve economic and nutritive efficiency.

关键词： ration formulation nutrient requirements objective programming linear programming feeding standard raw material nutritive equilibrium

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A View of Renqiu

Women of China 1999年第8期 27-29页

作者： wang GUANMIN,ZHAO jihua,SUN CHANGYUAN JIN JIE

Hatching and development of maize cyst nematode Heterodera zeae infecting different plant hosts

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Journal of Integrative Agriculture 2024年第5期23卷 1593-1603页

作者： Jiangkuan Cui Haohao Ren Bowang Fujie Chang Xuehai Zhang Haoguang Meng Shijun Jiang jihua Tang College of Plant Protection National Key Laboratory of Wheat and Maize Crop ScienceHenan Agricultural UniversityZhengzhou 450002China

The occurrence, distribution, and rapid molecular detection technology of Heterodera zeae Koshy et al. 1971, have been reported in China. We explored the biological characteristics of H. zeae sampled in Henan Province, China to understand its interaction with plants. Cysts and second-stage juveniles(J2s) were identified under an optical and scanning electron microscope, internal transcribed spacer(ITS) phylogenetic tree, and sequence characterized amplified region(SCAR)-PCR analyses. The optimum hatching temperatures of H. zeae were 30°C and 28°C, with cumulative hatching rates of 16.5 and 16.1%, respectively, at 30 days post-hatching(dph). The hatching rate of H. zeae eggs was improved by 20-and 50-time maize soil leachate and root juice, and 10-time root exudates. The hatching rate in 10-time root exudates was the highest(25.9%). The 10-time root exudates of maize and millet produced the highest hatching rate at 30 dph(25.9 and 22.9%, respectively), followed by wheat(19.9%), barley(18.3%), and rice(17.6%). Heterodera zeae developed faster in maize than in other crops. Fourth-stage juveniles(J4s) were detected in maize roots 8 days post-inoculation(dpi) at 28°C but not in other crops. Combined with hatching tests, the Huang–Huai–Hai summer maize region and the south and central-southwest mountainous maize areas are highly suitable for H. zeae in China. This is the first systematically study of the hatching and infection characteristics on different plant hosts of corn cyst nematode H. zeae in temperate regions. This study laid a theoretical foundation for the rapid spread and high environmental adaptability of corn cyst nematode.

关键词： crop host resistance Heterodera zeae incubation fluid maize cyst nematode nematode development nematodehatching

The impact of ^(60)Co γ-ray on apoptosis to Hep-2 human laryngeal cancer cell in the condition of reoxygenation after hypoxia

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The Chinese-German Journal of Clinical Oncology 2010年第2期9卷 93-96页

作者： Baoshan wang jihua Zhang Dongmei Song Ying Zhang Department of Otolaryngology-Head and Neck Surgery The First Hospital Hebei Medical University Shijiazhuang 050031 China

Objective： The aim of the study was to establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying, trying to investigate the impact of normoxia, hypoxia, reoxygenation after hypoxia on apoptosis and expression of proteins HIF-1α and p53 to Hep-2 human laryngeal cancer cell line induced by ^60Co γ-ray. Methods： Human laryngeal cancer Hep-2 cells were divided into 3 groups： group A （normoxia）, group B （hypoxia）, and group C （reoxygenation after hypoxia）. All of the cells were exposed to 5 Gy dosage of γ-ray. Flow cytometry （FCM） was used tomeasure the protein levels of HIF-1α and p53 and to detect cell apoptosis. The protein levels of HIF-la and p53 were also determined by immunohistochemistry and Western blotting. The expression of HIF-la mRNA was determined by RT-PCR. Results： The protein levels of HIF-1α and p53 were evidently increased in group B compared to group A. The protein levels of HIF-1α and p53 in group C were lower compared to group B; the rate of apoptosis in group C was higher than that in group B. Conclusion： Hypoxia decreased the effect of apoptosis induced by ^60Co γ-ray in Hep-2 human laryngeal cancer cell line. The apoptosis pathway maybe related to some other genes or proteins but not p53 in the conditions of hypoxia and reoxygenation after hypoxia.

关键词： γ-ray hypoxia apoptosis p53 Hep-2 cell