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葛藤花HPLC指纹图谱建立及3种成分含量测定

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中成药 2024年第8期46卷 2511-2516页

作者：王计瑞陈继建代金鑫杨锡金蒋燕青魏江平阳勇张小梅重庆市中药研究院国家中医药管理局中药化学三级实验室重庆400065 重庆医科大学中医药学院重庆401331 遵义医科大学药学院贵州遵义563000 重庆上药慧远药业有限公司重庆401331

目的建立葛藤花HPLC指纹图谱,并测定染料木苷、鸢尾苷、染料木素的含量。方法分析采用AQ-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长260 nm。再进行聚类分析和主成分分析... 详细信息

目的建立葛藤花HPLC指纹图谱,并测定染料木苷、鸢尾苷、染料木素的含量。方法分析采用AQ-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长260 nm。再进行聚类分析和主成分分析。结果10批药材指纹图谱中有17个共有峰,相似度大于0.970。3种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率100.50%~104.30%,RSD 1.93%~5.21%。各批药材聚为2类,3种主成分累积方差贡献率为84.822%。结论该方法稳定可靠,可用于葛藤花的质量控制。

关键词：葛藤花 HPLC指纹图谱染料木苷鸢尾苷染料木素含量测定聚类分析主成分分析

Analysis of LMW-GS,α-and γ-Gliadin Gene Coding Sequences from Triticum macha

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Agricultural Sciences in China 2010年第2期9卷 163-169页

作者： XIONG Li-juan wang ji-rui ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University Ya＇an 625014 P.R.China Key Laboratory of Southwestern Crop Germplasm Utilization Ministry of Agriculture Ya＇an 625014 P.R.China

Novel LMW-GS （low molecular weight glutenin subunit）,α- and γ-gliadin from Triticum macha accessions were characterized via genomic PCR, which can do favor to improve the wheat quality. The complete coding regions of two α-gliadin, two γ-gliadin and two LMW-GS gene sequences, which designed as Gli-Mal, Gli-Ma2, Gli-Mrl, Gli-Mr2, Glu-LM1 and Glu-LM2, encoded the mature proteins with 307, 241, 348, 302, 474 and 377 amino acid residues, respectively. Gli-Mal and Gli-Ma2 were recognized as pseudogenes due to the in-frame stop codons. The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α- or γ-gliadin or LMW- m type proteins with the exception of Gli-Ma2. Phylogenetic analysis showed Gli-Mal was closely related to those from T. aestivum, whereas Gli-Ma2 seemed to be more homologous with the gene sequences from Dasypyrum breviaristatum. Gli-Mrl was closely related to those from T. turgidum ssp. dicoccoides, while Gli-Mr2 was the nearest to those from T. aestivum. Glu-LM1 was closely related to those from Aegilops tauschii, whereas GIu-LM2 seemed to be more homologous with those from T. durum.

关键词： T. macha α-gliadin γ-gliadin LMW-GS gene clone sequence analysis

小麦农家种成株期条锈病抗性QTL定位及其育种效应解析

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作物学报 2024年第9期50卷 2167-2178页

作者：黄林玉张潇月李豪邓梅康厚扬魏育明王际睿蒋云峰陈国跃四川农业大学小麦研究所四川成都611130 西南作物基因资源发掘与利用国家重点实验室四川成都611130

条锈病是世界范围内严重影响小麦产量的重要病害。遗传和育种利用效应不清,加之不良性状连锁累赘是限制绝大多数已发掘小麦条锈病抗性基因在育种及生产中难以广泛应用的关键。前期研究发现,小麦农家种红芒麦子对我国当前小麦生产上流行... 详细信息

条锈病是世界范围内严重影响小麦产量的重要病害。遗传和育种利用效应不清,加之不良性状连锁累赘是限制绝大多数已发掘小麦条锈病抗性基因在育种及生产中难以广泛应用的关键。前期研究发现,小麦农家种红芒麦子对我国当前小麦生产上流行的主要条锈菌生理小种及致病类群具有稳定的成株期抗性。本研究通过构建Avocet S×红芒麦子F1及F2和F2:3家系,利用分离群体分组分析法(bulked segregation analysis, BSA)并结合小麦55K SNP芯片及外显子测序技术,在7A和7D染色体上鉴定到2个来自红芒麦子的成株期条锈病抗性主效QTL(***-7AL和***-7DS),分别解释了11.64%~15.25%和24.33%~40.58%的表型变异。标记连锁分析、遗传和物理图谱综合分析发现,***-7DS与已知成株期条锈病抗性基因Yr18呈高度共线性,表明该位点抗性效应来源于Yr18;而***-7AL是一个控制小麦成株期条锈病抗性潜在新位点,并进一步开发了可用于跟踪选择该位点的KASP(kompetitive allele-specific PCR,竞争性等位基因特异性PCR)分子标记。利用绵麦1618×红芒麦子BC_1F_2遗传改良群体对来自红芒麦子成株期条锈病抗性主效QTL遗传效应及其与产量相关性状协同改良效应进行解析。结果发现,在绵麦1618遗传背景下,Yr18和***-7AL的转育或聚合可显著降低条锈病危害,且对小麦穗长和分蘖数呈正向效应。上述研究结果表明,在小麦产量育种中可利用农家种红芒麦子进行成株期条锈病遗传改良。

关键词：小麦农家种成株期条锈病抗性基因红芒麦子遗传效应育种效应

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Development review and the prospect of oil shale in-situ catalysis conversion technology

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Petroleum Science 2024年第2期21卷 1385-1395页

作者： Li wang Chen-Hao Gao rui-Ying Xiong Xiao-Jun Zhang ji-Xiang Guo The Unconventional Oil and Gas Institute China University of Petroleum-BeijingBeijing102200China

As an unconventional resource, oil shale possesses abundant reserves and significant potential for industrial applications. The rational and efficient development of oil shale resources holds immense importance in reducing national energy demand. In-situ catalytic technology, characterized by its high efficiency, low pollution, and minimal energy consumption, represents a key direction for future oil shale development. This paper provides a comprehensive review of research progress in in-situ oil shale mining technology, oil shale pyrolysis catalysts, the pyrolysis mechanism of kerogen, and the compatibility of different heating processes and catalysts. Furthermore, the paper proposes future research directions and prospects for oil shale in-situ catalytic technology, including reservoir modification, highefficiency catalyst synthesis, injection processes, and high-efficiency heating technology. These insights serve as valuable technical references for the advancement of oil shale in-situ catalytic technology.

关键词： Oil shale In-situ catalytic technology Pyrolysis catalyst Kerogen pyrolysis mechanism

In situ modification of heavy oil catalyzed by nanosized metal-organic framework at mild temperature and its mechanism

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Chinese Journal of Chemical Engineering 2024年第3期67卷 166-173页

作者： Li wang ji-Xiang Guo rui-Ying Xiong Chen-Hao Gao Xiao-Jun Zhang Dan Luo The Unconventional Oil and Gas Institute China University of Petroleum-BeijingBeijing 102200China Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 101400China

Two catalysts, nano-sized cobalt-metal-organic framework(Co-MOF) and nickel(Ni)-MOF, were successfully prepared by the modification method. Tetralin(C10H12) was used as the hydrogen donor for the catalytic cracking and hydrogenation modification study of the dehydrated crude oil from the Shengli Oilfield. The optimal reaction conditions were determined through orthogonal experiments, and the components of the crude oil and modified oil samples were analyzed. The results revealed that the nanoMOF catalysts were successfully prepared and exhibited high catalytic activity. They could catalyze the cracking of large molecules in heavy oil at mild temperatures(<300°C), leading to the decomposition of the hydrogen donor. When the mass fraction of the catalyst was 0.2%, the mass fraction of the hydrogen donor was 1%, and the reaction temperature was 280°C, the Ni-MOF showed the best catalytic viscosity reduction effect. It could reduce the viscosity of heavy oil at 50°C from 15761.9 m Pa.s to 1266.2 m Pa.s,with a viscosity reduction rate of 91.97%. The modification effect of Co-MOF was the next best, which could reduce the viscosity of heavy oil to 2500.1 m Pa.s with a viscosity reduction rate of 84.14%. Molecular dynamics simulations revealed a strong interaction force between the MOF surface and asphaltene molecules. In the process of heavy-oil catalytic hydrogenation, the nano-MOF catalyst exhibited high catalytic activity. On the one hand, the empty d orbitals outside the metal atoms in the catalyst could polarize the carbon atoms in the organic matter, accelerating the breaking of long chains. On the other hand, the metal atoms in the catalyst could bond with the carbon σ bonds, breaking the carbon-carbon bonds. This disrupted the structure of the recombined components in the crude oil, irreversibly reducing the viscosity of the heavy oil and improving its fluidity.

关键词： Nano-sized MOF Petroleum Viscosity reduction Catalyst Nanomaterials

Light People: Professor Fanglin Bao and his cover story

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Light(Science & Applications) 2024年第6期13卷 1015-1020页

作者： ji wang School of Science Westlake UniversityHangzhouZhejiangPR China

Qu Yuan,a renowned ancient Chinese poet,once pondered in his work Heavenly Inquiry:“If the sun’s light is absent,from where does the radiance of the Hero Flower emanate?”The ability to see clearly in the darkest of nights has long been a sought-after magical power by *** holds not only immense technological significance for computer vision and remote sensing but also profound implications for transcending the dichotomy between day and night in our daily *** Fanglin Bao from Westlake University has made significant breakthroughs in this field,bringing us closer to a world where we can transform night into *** groundbreaking research on Heat-Assisted Detection and Ranging(HADAR)and night vision was featured as the cover story in Nature on July 26,2023.

关键词： bringing breakthrough holds

Origin and tectonic setting of low-Si alkaline magma

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Science China Earth Sciences 2024年第5期67卷 1417-1435页

作者： Qiushi ZHOU rui wang State Key Laboratory of Geological Processes and Mineral Resources and Institute of Earth SciencesChina University of GeosciencesBeijing 100083China Frontiers Science Center for Deep-time Digital Earth China University of Geosciences(Beijing)Beijing 100083China

Despite of the relatively rare occurrence of alkaline igneous rocks on Earth, they are the most diverse group of igneous rocks due to variations in their mineral assemblages and geochemical compositions. Alkaline igneous rocks are generally characterized by Si undersaturation, and K-Na(and in some cases C) enrichment. Although there is no unified alkaline igneous rock classification scheme, diagrams such as total alkalis-SiO_(2) or K_(2)O-SiO_(2) can be used to classify alkaline igneous rocks. The origins of silica-saturated alkaline rocks(SiO_(2)>52 wt.%) are relatively complex because multiple episodes of magmatism are often involved, therefore most studies have focused on the origins of silica-undersaturated alkaline *** experimental petrological studies have been conducted in the past few decades. Initially, it was considered that enrichment of trace elements was only achieved by low-degree partial melting of mantle peridotite, but the experimental melts could not reproduce the geochemical composition of natural alkaline igneous rocks. Subsequent studies have focused on carbonate-bearing eclogites that represent the average component of subducted oceanic crust. Although experimental studies indicate that silica-undersaturated and alkali-oversaturated melts can be generated from eclogites, some studies have considered that natural silica-undersaturated alkaline igneous rocks are the result of multi-stage source enrichment by incompatible elements. Low-degree partial melts of the mantle can be emplaced in the lithospheric mantle, forming veins consisting of phlogopite, amphibole, and pyroxene. Melting experiments including these components show that the produced melts better reproduce the naturally occurring silica-undersaturated alkaline magmas. Silica-deficient alkaline igneous rocks occur in various tectonic settings. Those in intraplate and divergent settings(i.e., mainly continental rifts) normally involve contributions from recycled components su

关键词： Alkaline igneous rock Tectonic setting Petrogenesis Big data

Effects of organic fertilizers on organic carbon accumulation in alkalized saline soil and silage maize yield

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International Journal of Agricultural and Biological Engineering 2024年第2期17卷 159-168页

作者： Lidong ji Lei Li rui wang Xing Xu Fengju Zhang Guilian Mao Teng wang School of Forestry and Prataculture Ningxia UniversityYinchuan750021China School of Agriculture Ningxia UniversityYinchuan750021China School of Ecological Environment Ningxia UniversityYinchuan 750021China School of Life Sciences Ningxia UniversityYinchuan 750021China

To understand the combined effect of organic and chemical fertilizers on soil carbon emissions and carbon balance of a farmland ecosystem,this study investigated the organic fertilizer nitrogen replacing different proportions of chemical fertilizer *** results showed that,compared to F_(100),the O_(15)F_(85) treatment increased the yield and net ecosystem productivity carbon sequestration of silage maize under mild,moderate,and severe salinization levels,as well as the contents of soil organic carbon,microbial carbon,and humin carbon,while reducing plant carbon *** O_(15)F_(85) treatment did not significantly increase soil carbon emissions(CEC),but O_(30)F_(70),O_(45)F_(55) and O_(100) treatments significantly increased *** soil carbon balance analysis showed that the farmland ecosystem was a“sink”for atmospheric CO_(2) under each *** O_(15)F_(85) treatment produced an“excitation effect”to enhance the carbon sink effect of silage maize farmland under mild,moderate and severe salinization levels while maintaining stable production and *** the O_(100) treatment increased the carbon sink of farmland under different salinization levels,the yield was significantly reduced and did not represent practical production *** analysis showed that soil organic carbon components and ecosystem carbon balance were closely related to soil total salt,pH and bulk density,while soil dissolved organic carbon,humus carbon components and carbon emissions were closely related to soil moisture and ***,the purpose of improving the carbon sink of saline-alkali land can be achieved through soil salt inhibition,soil structure remodeling and water supplement and warming regulation,which provides technical and theoretical support for reducing carbon emissions,achieving carbon neutrality and alleviating global warming.

关键词： carbon balance carbon component organic carbon organic substitution saline-alkali land

Investigation on combustion and emission characteristics of diesel polyoxymethylene dimethyl ethers blend fuels with exhaust gas recirculation and double injection strategy

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Journal of Traffic and Transportation Engineering(English Edition) 2024年第4期11卷 614-630页

作者： Xin Su rui Su Nan Gao Hao Chen Zhenhua ji Hongming Xu Biao wang Shaanxi Key Laboratory of New Transportation Energy and Automotive Energy Saving Chang'an UniversityXi'an 710064China School of Vechicle Engineering Xi'an Aeronautical UniversityXi'an 710077China Department of Mechanical Engineering University of BirminghamBirmingham B152TTUK

As a kind of renewable and high oxygen content fuel,polyoxymethylene dimethyl ether(PODE)can be added in diesel to realize energy saving and emissions *** evaluate the combustion and emission characteristics of a diesel engine fueled with diesel and diesel/PODE mixtures,exhaust gas recirculation(EGR)and main-pilot injection strategies with various injection timings were *** was blended with diesel by volume to form mixtures which were marked as D100(pure diesel),D90P10(90%diesel+10%PODE),and D80P20(80%diesel+20%PODE).The results showed that the ignition delay(ID)and combustion duration(CD)of D80P20 were the shortest because of the highest cetane number(CN)and high oxygen content of PODE,indicating more concentrated heat *** low and medium loads,D80P20 achieved the highest peak heat release ratio(PHRR)and peak combustion temperature(PCT)among the three fuels,and it was 14.3%and 3.6%higher than those of *** blending with diesel can significantly reduce particulate matter(PM)and D80P20 has the lowest PM emissions at all *** with D100,both PM and nitrogen oxide(NO_(x))emissions of PODE blends decreased simultaneously with 20%EGR at all *** the increase of pilot-main interval,the ID and CD of all test fuels increased,while the NO_(x)and PM emissions *** conclusions of the present research provide a state of the application in light-duty engines fueled with diesel/PODE blends in future work.

关键词： Polyoxymethylene dimethyl ethers Diesel engine NOx Particulate number Pilot plus main injections