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Chinese Journal of Chemical Engineering 2015年第5期23卷 835-841页

作者： Qilong Ge Xiuping Yue guoying wang School of Environmental Science and Engineering Taiyuan University of Technology

A strain capable of phenol degradation, hetemtrophic nitrification and aerobic denitrification was isolated from activated sludge of coking-plant wastewater ponds under aerobic condition. Based on its morphology, physiology, biochemical analysis and phylogenetic characteristics, the isolate was identified as Diaphorobacter sp. PD-7. Biodegradation tests of phenol showed that the maximum phenol degradation occurred at the late phase of exponential growth stages, with 1400 mg·L^-1 phenol completely degraded within 85 h. Diaphorobacter sp. PD-7 accumulated a vast quantity of phenol hydroxylase in this physiological phase, ensuring that the cells quickly utilize phenol as a sole carbon and energy source. The kinetic behavior ofDiaphorobacter sp. PD-7 in batch cultures was investigated over a wide range of initial phenol concentrations （0-1400mg·L^-1） by using the Haldane model, which adequately describes the dynamic behavior of phenol biodegradation by strain Diaphombacter sp. PD-7. At initial phenol concentration of 1400mg· L^-l, batch experiments （0.25 L flask） of nitrogen removal under aerobic condition gave almost entirely removal of 120.69mg· L^- 1 ammonium nitrogen within 75 h, while nitrate nitrogen removal reached 91% within 65 h. Moreover, hydroxylamine oxidase, periplasmic nitrate reductase and nitrite reductase were successfully expressed in the isolate.

关键词： Diaphorobacter sp.Phenol biodegradation Kinetics Heterotrophic nitrification Aerobic denitrification Enzyme activity

Hydroxyl,hydroperoxyl free radicals determination methods in atmosphere and troposphere

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Journal of Environmental Sciences 2021年第1期33卷 324-335页

作者： guoying wang Yves Iradukunda Gaofeng Shi Pascaline Sanga Xiuli Niu Zhijun Wu School of Petrochemical Engineering Lanzhou University of TechnologyLanzhou 730050China Gansu Food Inspection and Research Institute Lanzhou 730050China State Key Joint Laboratory of Environment Simulation and Pollution Control(Peking University) College of Enuironmental Sciences and EngineeringPeking UniversityBeijing 100871China

The hydroxyl radical(·OH)has a crucial function in the oxidation and removal of many atmospheric compounds that are harmful to ***,high reactivity,low atmospheric abundance,determination of hydroxyl,and hydroperoxyl radical’s quantity is very *** the atmosphere and troposphere,hydroperoxyl radicals(HO_(2))are closely demanded in the chemical oxidation of the *** advances in technology have allowed researchers to improve the determination methods on the research of free radicals through some spectroscopic *** far,several methods such as laser-induced fluorescence(LIF),high-performance liquid chromatography(HPLC),and chemical ionization mass spectroscopy have been identified and mostly used in determining the quantity of hydroxyl and hydroperoxyl *** this systematic review,we have advised the use of scavenger as an advance for further researchers to circumvent some of these problems caused by free *** primary goal of this review is to deepen our understanding of the functions of the most critical free radical(·OH,HO_(2))and also understand the currently used methods to quantify them in the atmosphere and troposphere.

关键词： Atmosphere Determination method Hydroxyl radical Hydroperoxyl radical Troposphere

CD59 Silencing via Retrovirus-Mediated RNA Interference Enhanced Complement-Mediated Cell Damage in Ovary Cancer

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Cellular & Molecular Immunology 2009年第1期6卷 61-66页

作者： Xuexiang Shi Bei Zhang Jinlin Zang guoying wang Meihua Gao Department of Immunology Medical College of Qingdao University Qingdao 266071 China Department of Immunization Programme Qingdao Municipal Center for Disease Control and Prevention Qingdao 266033 China Department of Surgery Qingdao Municipal Hospital Qingdao 266021 China

CD59, belonging to membrane complement regulatory proteins （mCRPs）, inhibits the cytolytic activity of complement and is over-expressed in solid cancers, including ovary cancer. The aim of the present study was to construct recombinant retrovirus encoding shRNA targeted human CD59 and infect A2780 cells in order to investigate the relationship between decreased CD59 expression and tumorigenesis of ovary cancer, siCD59 and siCD59-C were successfully constructed and identified by PCR, restriction endonuclease analyses and DNA sequencing, respectively. The siCD59 was able to efficiently infect A2780 cells, which was confirmed by Western blotting. When incubated with fresh normal human serum （8%, v/v） for 1 h at 37℃, the cell viability was decreased and cell damage was increased in siCD59 infected A2780 cells compared to siCD59-C infected cells. This led to the activation of caspase-3. The apoptosis in siCD59 infected cells was shown with hypercondensed nuclei using Hoechst staining. Meanwhile, the weight of ovary tumor graft in nude mice was significantly decreased in siCD59 group compared to that of siCD59-C group. And the expression of CD59 protein in tumor tissue in siCD59 group was significantly decreased. These results suggested that CD59 silencing in ovary cancer cells v/a retrovirusmediated RNAi can enhance complement-mediated cell damage, inhibiting growth of ovary cancer. CD59 might be a potential target for gene therapy in ovary cancer. Cellular ＆ Molecular Immunology.

关键词： CD59 complement RNA interference gene therapy ovary cancer

Virus-induced silencing of a tobacco deoxyhypusine synthase gene

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Chinese Science Bulletin 2005年第23期50卷 2707-2713页

作者： wang Hongzhi MA Rongcai LI Ruifen wang guoying WEI Jianhua State Key Laboratory for Agrobiotechnology China Agricultural University Beijng 100094 China Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100089 China

A cDNA fragment corresponding to deoxyhy-pusine synthase gene NbDHS was isolated and cloned into potato virus X (PVX) vector for functional analysis in Nico-tiana benthamiana by using virus-induced gene silencing (VIGS). Plants agroinfected with recombinant virus vector PVX-NbDHS exhibited an increase in leaf biomass, delay in natural leaf senescence and flowering time, and decrease in leaf chlorophyll content. Semi-quantitative RT-PCR and Northern analysis showed that the transcript level of DHS was significantly lower in PVX-NbDHS infected plants. At the same time, the expression for eIF-5A, the target proteins of DHS in N. benthamiana, was concomitantly suppressed by semi-quantitative RT-PCR and Western analysis. From the phenotypic feature of the infected plants and the reduced expression abundance of DHS and eIF-5A, we concluded that NbDHS plays important roles in plant growth, development and senescence. The possible application of DHS gene in ge-netic modification of crops and horticultural plants was dis-cussed.

关键词：烟草脱氧羟腐胺缩赖氨酸合酶基因基因克隆生长促进作用

Effect of long reaction distance on gas composition from organic-rich shale pyrolysis under high-temperature steam environment

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International Journal of Coal Science & Technology 2024年第3期11卷 102-119页

作者： Lei wang Rui Zhang guoying wang Jing Zhao Dong Yang Zhiqin Kang Yangsheng Zhao Key Laboratory of In-Situ Property Improving Mining of Ministry of Education Taiyuan University of TechnologyTaiyuan030024China The In-Situ Steam Injection Branch of State Center for Research and Development of Organic-Rich Shale Exploitation Taiyuan University of TechnologyTaiyuan030024China College of Mining Engineering Taiyuan University of TechnologyTaiyuan030024China School of Energy Science and Engineering Henan Polytechnic UniversityJiaozuo454002China

When high-temperature steam is used as a medium to pyrolyze organic-rich shale,water steam not only acts as heat transfer but also participates in the chemical reaction of organic matter pyrolysis,thus affecting the generation law and release characteristics of gas *** this study,based on a long-distance reaction system of organic-rich shale pyrolysis via steam injection,the effects of steam temperature and reaction distance on gas product composition are analyzed in depth and compared with other pyrolysis *** advantages of organic-rich shale pyrolysis via steam injection are then *** volume concentration of hydrogen in the gas product obtained via the steam injection pyrolysis of organic-rich shale is the highest,which is more than 60%.The hydrogen content increases as the reaction distance is extended;however,the rate of increase changes *** the reaction distance from 800 to 4000 mm increases the hydrogen content from 34.91%to 69.68%and from 63.13%to 78.61%when the steam temperature is 500℃ and 555℃,***,the higher the heat injection temperature,the smaller the reaction distance required to form a high concentration hydrogen pyrolysis environment(hydrogen concentration>60%).When the steam pyrolysis temperature is increased from 500℃ to 555℃,the reaction distance required to form a high concentration of hydrogen is reduced from 3800 to 800 *** with the direct retorting process,the volume concentration of hydrogen obtained from high-temperature steam pyrolysis of organic-rich shale is 8.82 and 10.72 times that of the commonly used Fushun and Kivite furnaces,*** pyrolysis of organic-rich shale via steam injection is a pyrolysis process in a hydrogen-rich environment.

关键词： Steam temperature Pyrolysis gas Hydrogen-rich Reaction distance Direct retorting

Using Interactome Big Data to Crack Genetic Mysteries and Enhance Future Crop Breeding

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Molecular Plant 2021年第1期14卷 77-94页

作者： Leiming Wu Linqian Han Qing Li guoying wang Hongwei Zhang Lin Li National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China lnstitute of Crop Science Chinese Academy of Agricultural SciencesBeijing 100081China

The functional genes underlying phenotypic variation and their interactions represent“genetic mysteries”.Understanding and utilizing these genetic mysteries are key solutions for mitigating the current threats to agriculture posed by population growth and individual food *** to advances in highthroughput multi-omics technologies,we are stepping into an Interactome Big Data era that is certain to revolutionize genetic *** this article,we provide a brief overview of current strategies to explore genetic *** then introduce the methods for constructing and analyzing the Interactome Big Data and summarize currently available interactome ***,we discuss how Interactome Big Data can be used as a versatile tool to dissect genetic *** propose an integrated strategy that could revolutionize genetic research by combining Interactome Big Data with machine learning,which involves mining information hidden in Big Data to identify the genetic models or networks that control various traits,and also provide a detailed procedure for systematic dissection of genetic mysteries,Finally,we discuss three promising future breeding strategies utilizing the Interactome Big Data to improve crop yields and quality.

关键词： genetic mystery interactome big data machine learning crop breeding

Seasonal variation characteristics of hydroxyl radical pollution and its potential formation mechanism during the daytime in Lanzhou

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Journal of Environmental Sciences 2020年第9期32卷 58-64页

作者： guoying wang Shiming Jia Ruihong Li Shangrong Ma Xuefu Chen Zhijun Wu Gaofeng Shi Xiuli Niu School of Petrochemical Engineering Lanzhou University of TechnologyLanzhou 730050China State Key Joint Laboratory of Environment Simulation and Pollution Control(Peking University) College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China Gansu Food Inspection and Research Institute Lanzhou 730030China

Hydroxyl free radicals(OH radicals) play the main role in atmospheric chemistry and their involving reactions are the dominant rate determining step in the formation of secondary fine particulate matter and in the removal of air pollutants from the *** this paper,we studied the seasonal variation characteristics of OH radicals during the daytime in Lanzhou and explored the potential formation mechanism of high concentration OH *** found that the OH radicals in four seasons was 2.7×106,2.6×106,3.1×106, and2.2×106 cm-3,*** the rainfall was concentrated in summer,the wet deposition had a significant effect on removing OH *** the four pollutants(including ozone(O3),volatile organic compounds(VOCs),nitrogen dioxide(NO2) and fine particulate matter(PM2.5)),the variation of OH radicals were closely related to ozone concentration especially in spring and *** autumn,the correlation between PM2.5 and OH radicals were the closest among the observing pollutants and its formation mechanism was different conventional regeneration *** Event 1,high concentration of ozone was the main source of OH radicals;under the high humidity condition,except for ozone,the multiple factors including VOCs,NO2 and PM2.5 interplayed and leaded to the Event 2.

关键词： pollution ozone radicals

Transgenic tobacco plants expressing synthetic Cry1Ac and Cry1Ie genes are more toxic to cotton bollworm than those containing one gene

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Chinese Science Bulletin 2008年第9期53卷 1381-1387页

作者： LIAN Yun JIA ZhiWei HE KangLai LIU Yundun SONG FuPing wang BaoMin wang guoying State Key Laboratory of Agrobiotechnology China Agricultural University Beijing 100094 China State Key Laboratory for Biology of Plant Diseases and Insect Pests institute of Plant Protection Chinese Academy of Agriculture Sciences Beijing 100094 China Institute of Crop Sciences Chinese Academy of Agricalture Sciences Beijing 100081 China

Transgenic tobacco plants carrying Cry1Ac, Cry1Ie or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1Ie proteins were 0.173% and 0.131% of the total proteins, respectively. Cry1Ac protein content was 0.182% and Cry1Ie protein con- tent was 0.124% of the total proteins in the leaves of transgenic plants containing only one Bt gene. Fresh leaves of transgenic tobacco and wild-type plants were used for the insect bioassay against wild-type and Cry1Ac-resistant cotton bollworm (Helicoverpa armigera). The bioassay results showed that transgenic plants carrying both genes were significantly more toxic to wild-type and Cry1Ac-resistant cotton bollworm than those carrying Cry1Ac or Cry1Ie alone. This study indicates that the higher toxicity of transgenic tobacco plants carrying both genes is caused by the cooperative function of both Bt proteins, thus providing a potential way to delay the development of insect resis- tance to transgenic crops.

关键词：锕烟草中毒棉花螟蛉

Construction of a genetic map and location of quantitative trait loci for dwarf trait in maize by RFLP markers

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Chinese Science Bulletin 2000年第3期45卷 247-250页

作者： CAO Yongguo wang guoying wang Shoucai WEI Yanling LU Jiang XIE Youju DAI Jingrui College of Plant Science and Technology Chinese Agricultural UniversityBeijing 100094China National Laboratories for Agrobiotechnology Chinese Agricultural UniverysityBeijing 100094China Florida A&M University TallahasseeFL 32307USA

Using F2 population derived from the cross of tall inbred 7922 by dwarf inbred 5003, an RFLP linkage map of maize has been constructed, on which 85 markers are distributed among 10 linkage groups and span maize genome about 1827.8 cM with an average distance (24.4 cM) between markers. 106 F2:3 lines of the population were grown in a 10×11 simple rectangular lattice design of one-raw plots with two replications and evaluated for plant height (PH). With interval mapping procedure, 5 QTLs controlling plant height have been identified and their genetic effects and gene action determined. 2 major OTLs with opposite effect have been discovered. One for increasing plant height is ph 1 which is located at chromosome 2 and accounts for 51.8% of the total phenotypic variation; the other for decreasing plant height is ph3 which is located at chromosome 5 and accounts for 38.6% of the total phenotypic variation. The chromosomal location of ph3 might be the same as or close to the position of bv1, a dwarf mutant

关键词： Zea mays L. RFLP genetic map dwarf trait major QTL.

Size-transformable nanoparticles with sequentially triggered drug release and enhanced penetration for anticancer therapy

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Nano Research 2023年第8期16卷 11186-11196页

作者： Yulin Li Liudi wang Guoqiang Zhong guoying wang Yanzhao Zhu Jian Li Lan Xiao Yanhui Chu Yan Wu Kaichun Li Jie Gao Engineering Research Centre for Biomedical Materials of Ministry of Education The Key Laboratory for Ultrafine Materials of Ministry of EducationFrontiers Science Center for Materiobiology and Dynamic ChemistrySchool of Material Science and EngineeringEast China University of Science and TechnologyShanghai 200237China Department of Oncology Shanghai Fourth People’s Hospital School of MedicineTongji UniversityShanghai 200434China Changhai Clinical Research Unit Shanghai Changhai HospitalNaval Medical UniversityShanghai 200433China College of Life Sciences Mudanjiang Medical University Mudanjiang 157011China Wenzhou Institute of Shanghai University Wenzhou 325000China Centre for Biomedical Technologies Queensland University of TechnologyBrisbaneQLD 4059Australia

There are several limitations to the application of nanoparticles in the treatment of cancer,including their low drug loading,poor colloidal stability,insufficient tumor penetration,and uncontrolled release of the ***,gelatin/laponite(LP)/doxorubicin(GLD)nanoparticles are developed by crosslinking LP with gelatin for doxorubicin *** shows high doxorubicin encapsulation efficacy(99%)and strong colloidal stability,as seen from the unchanged size over the past 21 days and reduced protein absorption by 48-fold compared with unmodified laponite/doxorubicin *** gelatin from 115 nm GLD reaches the tumor site,matrix metallopeptidase-2(MMP-2)from the tumor environment breaks it down to release smaller 40 nm LP nanoparticles for effective tumor cell *** demonstrated by superior penetration in both in vitro three-dimensional(3D)tumor spheroids(138-fold increase compared to the free drug)and in vivo tumor *** intracellular low pH and MMP-2 further cause doxorubicin release after endocytosis by tumor cells,leading to a higher inhibitory potential against cancer *** improved anticancer effectiveness and strong in vivo biocompatibility of GLD have been confirmed using a mouse tumor-bearing ***-2/pH sequentially triggered anticancer drug delivery is made possible by the logical design of tumor-penetrating GLD,offering a useful method for anticancer therapy.

关键词： sequentially triggered drug release size-transformable nanoparticles tumor penetration anticancer therapy drug delivery