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Modified vitrification method for cryopreservation of human ovarian tissues

Chinese Medical Journal 2007年第2期120卷 110-114页

作者： LI yu-bin ZHOU Can-quan YANG Guo-fen wang Qiong dong yu Center of Reproductive Medicine First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080 China

Background Vitrification is a prospective technology in ovarian tissue cryopreservation, but it is still in an initial stage. This study was conducted to investigate a modified vitrification protocol for human ovarian tissue, which can be used as an alternative to preserve fertility for young women with cancer who have to undergo cytotoxic therapy and sterilization. Methods Ovarian tissue samples were collected from 15 patients and randomly allocated to groups of fresh, vitrification, and conventional slow freezing. A modified carrierless vitrification method was applied. The proportion of morphologically intact follicles in fresh ovarian tissues was compared with that in warmed/thawed tissues. The initial growth of the follicles and the concentrations of estradiol and progesterone were detected to determine the viability and endocrine function of the cryopreserved tissues. Results The proportion of morphologically intact primordial follicles in the fresh group （97.6%） was significantly higher than that in the other two groups （vitrification group 80.3% and slow-freezing group 72.6%, P〈0.001）. In both the vitrification and slow-freezing groups, estradiol and progesterone were secreted continuously during 2-week culture in vitro, the proportion of primary follicles were both significantly increased compared to the fresh group. No statistically significant differences existed between the two groups after cryopreservation in the proportion of both primordial and primary follicles, and the concentrations of estradiol and progesterone （P〉0.05）.Conclusion The modified vitrification method for cryopreservation of human ovarian tissues is effective, simple, and inexpensive.

关键词： cryopreservation vitrification ovarian tissue

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Chinese accounting reform： It will be a continuous process

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Journal of Modern Accounting and Auditing 2008年第8期4卷 38-41页

作者： wang dong-mei yu Qin-qin School of Management Graduate University of Chinese Academy of Sciences Beijing 100190 China

Based on a thorough review, this paper points out that there is still two types of problems in the newly formulated China Accounting Standards 2006 （CAS 2006） which were enacted on Jan. 1, 2007. The first type is that some economic events have been accounted for incorrectly, and the second is confusion and misunderstanding have been caused in the practical world when some guidelines of CAS 2006 are carded out to converge to the International Financial Reporting Standards （IFRS）. In my opinion, it is a great leap to establish a set of accounting standards that harmonize with the IFRS, but China＇s accounting reform is a continuously improving process. During the process, the accounting standards setting departments shoulder heavy burden, face a dilemma, and meet some challenges. After all, China＇s accounting reform will be a long way.

关键词： accounting standards problems dilemma improvement

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GROUND BOUNCING NOISE REDUCTION TECHNIQUE CONSIDERING WAKE-UP DELAY IN MTCMOS CIRCUITS

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Journal of Electronics(China) 2011年第4期28卷 596-601页

作者： Tian Xi wang yu dong Zaiwang Department of Electronic Engineering Tsinghua University Beijing 100084 China

Multi-Threshold CMOS(MTCMOS) is an effective technique for controlling leakage power with low delay *** the large magnitude of ground bouncing noise induced by the sleep to active mode transition may cause signal integrity problem in MTCMOS *** propose a methodology for reducing ground bouncing noise under the wake-up delay *** improved two-stage parallel power gating structure that can suppress the ground bouncing noise through turn on sets of sleep transistors consecutively is *** size of each sleep transistor is optimized by a novel sizing algorithm based on a simple discharging *** results show that the proposed techniques achieve at least 23% improvement in the product of the peak amplitude of ground bouncing noise and the wake-up time when compared with other existing techniques.

关键词： Ground bouncing noise Multi-Threshold CMOS(MTCMOS) Wake-up time Low leakage

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Spatial Interpolation and Sample Size Optimization for Soil Copper(Cu) Investigation in Cropland Soil at County Scale Using Cokriging

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Agricultural Sciences in China 2009年第11期8卷 1369-1377页

作者： PANG Su,LI Ting-xuan,wang Yong-dong,yu Hai-ying and LI Xi College of Resource and Environmental Science,Sichuan Agricultural University,Ya’an 625014,P.R.China [a]College of Resource and Environmental Science Sichuan Agricultural University Ya'an 625014 P.R. China

Knowledge on spatial distribution and sampling size optimization of soil copper （Cu） could lay solid foundations for environmetal quality survey of agricultural soils at county scale. In this investigation, cokriging method was used to conduct the interpolation of Cu concentraiton in cropland soil in Shuangliu County, Sichuan Province, China. Based on the original 623 physicochmically measured soil samples, 560, 498, and 432 sub-samples were randomly selected as target variable and soil organic matter （SOM） of the whole original samples as auxiliary variable. Interpolation results using Cokriging under different sampling numbers were evaluated for their applicability in estimating the spatial distribution of soil Cu at county sacle. The results showed that the root mean square error （RMSE） produced by Cokriging decreased from 0.9 to 7.77%, correlation coefficient between the predicted values and the measured increased from 1.76 to 9.76% in comparison with the ordinary Kriging under the corresponding sample sizes. The prediction accuracy using Cokriging was still higher than original 623 data using ordinary Kriging even as sample size reduced 10%, and their interpolation maps were highly in agreement. Therefore, Cokriging was proven to be a more accurate and economic method which could provide more information and benefit for the studies on spatial distribution of soil pollutants at county scale.

关键词： cropland soil county scale copper Cokriging sampling size

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Limited Role of Promoter Methylation of MGMT and C130RF18 in Triage of Low-Grade Squamous Intraepithelial Lesion

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Chinese Medical Journal 2018年第8期131卷 939-944页

作者： Lu-Lu Sun yuan Liu Xiao Sun Lei Pan Dan Wu yu-dong wang Department of Gynecological Oncology International Peace Maternity and Child Health Hospital of China Welfare Institute Shanghai 200030 China Department of Pathology International Peace Maternity and Child Health Hospital of China Welfare Institute Shanghai 200030 China

Background：Promoter methylation of MGMT and C13ORF18 has been confirmed as a potential biomarker for early diagnosis of cervical cancer. The aim of this study was to evaluate the performance of MGMT and C13ORF18 promoter methylation for triage of cytology screening samples and explore the potential ***：Methylation-sensitive high-resolution melting was used to detect promoter methylation of MGMT and C13ORF18 in 124 cervical samples. High-risk human papillomavirus （HR-HPV） was detected by the Digene Hybrid Capture 2？. Gene methylation frequencies in relation to cervical intraepithelial neoplasia （CIN） were analyzed. Frequencies were compared by Chi-square tests. The expression of gene biomarkers and methylation regulators was analyzed by immunohistochemical staining, real-time fluorescence quantitative polymerase chain reaction, and Western ***：For triage of low-grade squamous intraepithelial lesion （LSIL）, gene methylation increased specificity from 4.0% of HR-HPV detection to 30.8% of MGMT （χ2 = 9.873, P = 0.002） and to 50.0% of C13ORF18 （χ2 = 21.814, P = 0.001）. For triage of atypical squamous cells of undetermined significance, HR-HPV detection had higher positive predictive value of 54.8%. Either MGMT or C13ORF18 methylation combined with HR-HPV increased the negative predictive value to 100.0% （χ2 = 9.757, P = 0.002）. There was no relationship between MGMT and C13ORF18 expression and DNA methylation （χ2 = 0.776, P = 0.379 and χ2 = 1.411, P = 0.235, respectively）. MBD2 protein level in cervical cancer was relatively lower than normal cervical tissue （t = 4.11, P = 0.006）.Conclusions：HR-HPV detection is the cornerstone for triage setting of CIN. Promoter methylation of MGMT and C13ORF18 plays a limited role in triage of LSIL. Promoter methylation of both genes may not be the causes of gene silence.

关键词： Cervical Cancer Cervical Intraepithelial Neoplasia DNA Methylation

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Gene mutations of esophageal squamous cell carcinoma based on next-generation sequencing

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Chinese Medical Journal 2021年第6期134卷 708-715页

作者： Long wang Yi-Meng Jia Jing Zuo yu-dong wang Zhi-Song Fan Li Feng Xue Zhang Jing Han Wen-Jing Lyu Zhi-yu Ni Department of Oncology The Fourth Hospital of Hebei Medical UniversityShijiazhuangHebei 050011China The Affiliated Hospital of Hebei University BaodingHebei 071000China School of Basic Medical Science Hebei UniversityBaodingHebei 071000China

Background: Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers without effective therapy. To explore potential molecular targets in ESCC, we quantified the mutation spectrum and explored the relationship between gene mutation and clinicopathological characteristics and programmed death-ligand 1 (PD-L1) ***: Between 2015 and 2019, 29 surgically resected ESCC tissues and adjacent normal tissues from the Fourth Hospital of Hebei Medical University were subjected to targeted next-generation sequencing. The expression levels of PD-L1 were detected by immunohistochemistry. Mutational signatures were extracted from the mutation count matrix by using non-negative matrix factorization. The relationship between detected genomic alterations and clinicopathological characteristics and PD-L1 expression was estimated by Spearman rank correlation ***: The most frequently mutated gene wasTP53 (96.6%, 28/29), followed byNOTCH1 (27.6%, 8/29),EP300 (17.2%, 5/29), andKMT2C (17.2%, 5/29). The most frequently copy number amplified and deleted genes wereCCND1/FGF3/FGF4/FGF19 (41.4%, 12/29) andCDKN2A/2B (10.3%, 3/29). By quantifying the contribution of the mutational signatures to the mutation spectrum, we found that the contribution of signature 1, signature 2, signature 10, signature 12, signature 13, and signature 17 was relatively high. Further analysis revealed genetic variants associated with cell cycle, chromatin modification, Notch, and Janus kinase-signal transducer and activator of transcription signaling pathways, which may be key pathways in the development and progression of ESCC. Evaluation of PD-L1 expression in samples showed that 13.8% (4/29) of samples had tumor proportion score ≥1%. 17.2% (5/29) of patients had tumor mutation burden (TMB) above 10 mut/Mb. All samples exhibited microsatellite stability. TMB was significantly associated with lymph node metastasis (r = 0.468,P = 0.010), but not significantly associate

关键词： Esophageal squamous cell carcinoma Next-generation sequencing Mutational signature Programmed death-ligand 1

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基于曲线坐标系浸没边界方法的折流燃烧室模拟

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推进技术 2023年第5期44卷 118-130页

作者：王煜栋王方甘甜金捷北京航空航天大学能源与动力工程学院北京100191 北京航空航天大学江西研究院江西南昌330096 北京航空航天大学成都航空动力创新研究院四川成都611930

航空发动机折流燃烧室几何结构复杂,其高保真数值模拟需要高效的网格与边界条件处理方法。采用曲线坐标系隐式浸没边界方法结合大涡模拟-概率密度函数输运方程湍流燃烧模型开发自研软件,并实现WP11中折流燃烧室的高保真模拟。流动模拟... 详细信息

航空发动机折流燃烧室几何结构复杂,其高保真数值模拟需要高效的网格与边界条件处理方法。采用曲线坐标系隐式浸没边界方法结合大涡模拟-概率密度函数输运方程湍流燃烧模型开发自研软件,并实现WP11中折流燃烧室的高保真模拟。流动模拟中准确解析了该燃烧室中的三股主要气流,且三股气流分别约占进口流量的75%,12.5%和12.5%。两相燃烧模拟中针对拉格朗日框架下的液滴运动和欧拉框架下的湍流燃烧采用不同网格标记,模拟得到的出口径向温度分布规律与实验一致,平均相对误差为17.95%,表明基于本方法开发的自研软件能准确模拟折流燃烧室中的两相湍流燃烧现象。

关键词：数值模拟浸没边界方法大涡模拟概率密度函数输运方程湍流燃烧模型折流燃烧室

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Assessment of Comparative Genomic Hybridization Experiment by an in situ Synthesized CombiMatrix Microarray with Yersinia pestis Vaccine Strain EV76 DNA

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Biomedical and Environmental Sciences 2010年第5期23卷 384-390页

作者： yuAN-HAI YOU PENG wang YAN-HUA wang HAI-BIN wang dong-ZHENG yu RONG HAI JIAN-ZHONG ZHANG National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective The quality of microarray data influences the accuracy of comparative genomic analyses to a large *** ensure that the results obtained by using an in situ synthesized microarray are accurate,data quality is to be assessed by evaluating the melting temperature (Tm) of probes,probability of false synthesis rates,and fragmentation of labeled *** DNA from the Yersinia pestis vaccine strain EV76 was used for microarray *** results were confirmed by *** and bioinformatics methods were employed to perform microarray data analyses and *** Correlation coefficients of the three datasets were above 0.95 after two-time stripping and hybridization with a labeled DNA with the size of fragmentation being 200 bp-2 kb,which showed that the hybridization results were highly *** coefficients were lower with the values ranging from 0.87 to 0.92 between the datasets generated from hybridization with different sizes of the labeled DNA *** the relationship between Tm and signal intensity,there was a different distribution of Tm in the lowest 300 or 3 000 probes with a range of 70 ℃-72 ℃ and the highest 300 or 3 000 probes with a range of 72 ℃-74 ℃.Conclusion The results of this study suggest that the initial microarray design may affect the accuracy of final analyses and that the probe Tm and the size of the labeled fragment may be the two factors of the greatest importance.

关键词： Array CGH Data quality Assessment

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Dehydroepiandrosterone indirectly inhibits human osteoclastic resorption via activating osteoblastic viability by the MAPK pathway

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Chinese Medical Journal 2012年第7期125卷 1230-1235页

作者： wang yu-dong TAO Min-fang CHENG Wei-wei LIU Xiao-hua WAN Xiao-ping KeMi Cui Department of Gynecology International Peace Maternal and Child Health HospitalShanghai Jiao Tong UniversityShanghai 200030China

Background Dehydroepiandrosterone （DHEA） is widely known for its beneficial effect on postmenopausal osteoporosis, although the underlying mechanisms remain mainly unclear. In this study, we tried to determine the activation of mitogen-activated protein kinase signal pathways during DHEA treatment and the indirect role of osteoblasts （OBs） on osteoclasts under the DHEA treatment of postmenopausal osteoporosis. Methods Primary human OBs and osteoclast-like cells were cultured and, we pretreated OBs with or without U0126 （a highly selective inhibitor of both MEK1 and MEK2）. The OBs were treated with DHEA. We then tested the effects of DHEA on human osteoblastic viability, osteoprotegerin production and the expression of phosphor-ERK1/2 （extracellular signal-regulated kinase）. In the presence or absence of OBs, the function of osteoclastic resorption upon DHEA treatment was calculated. Results DHEA promoted the human osteoblastic proliferation and inhibited the osteoblastic apoptosis within the concentration range of 108-106 mol/L （P 〈0.05, P 〈0.01, respectively）. Within the effective concentration range, the expression of phosphor-ERK1/2 and osteoprotegerin was increased by DHEA and blocked by U0126. In the presence of OBs, DHEA could significantly decrease the number and the area of bone resorption lacuna （P 〈0.05 and P 〈0.01, respectively）. Without OBs, however, the effects of DHEA on the bone resorption lacuna were almost completely abolished. Conclusions DHEA could indirectly inhibit the human osteoclastic resorption through promoting the osteoblastic viability and osteoprotegerin production, which is mediated by mitogen-activated protein kinases signal pathway involving the phosphor-ERK1/2.

关键词： dehydroepiandrosterone osteoblasts osteoclast MAP kinase signaling pathways

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详细化学反应机理对预混丙烷钝体熄火模拟影响

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推进技术 2021年第10期42卷 2295-2305页

作者：王方韩宇轩窦力王煜栋金捷北京航空航天大学能源与动力工程学院航空发动机数值仿真研究中心北京100191

航空发动机熄火预测是重要关键问题之一,湍流和化学反应的非线性相互作用使预测非常困难。本文采用大涡模拟(LES)对湍流进行高精度模拟,采用概率密度函数输运方程湍流燃烧模型(TPDF)耦合JL4,Z66和H73三种化学反应机理,对预混丙烷钝体熄... 详细信息

航空发动机熄火预测是重要关键问题之一,湍流和化学反应的非线性相互作用使预测非常困难。本文采用大涡模拟(LES)对湍流进行高精度模拟,采用概率密度函数输运方程湍流燃烧模型(TPDF)耦合JL4,Z66和H73三种化学反应机理,对预混丙烷钝体熄火现象和规律进行研究。JL4的反应机理最简单,反应释热快,局部放热高,火焰宽度大,火焰两侧温度梯度大,燃烧更加趋于稳定,无法模拟出熄火状态。H73机理绝热火焰温度低,火焰温度低,回流区中部OH含量高;在近熄火状态,大量CO被氧化,释放热量过高导致无法模拟出熄火现象。Z66机理可以模拟出火焰正常状态,在低当量比下也可以模拟出熄火状态。算例中,局部Da>1的区域超过35%则会发生熄火。

关键词：概率密度函数输运方程湍流燃烧模型(TPDF) 大涡模拟(LES) 钝体熄火反应机理 AECSC程序

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