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Amino acid substitutions of GLY98, LEU245 and GLU543 in COIl distinctively affect jasmonate-regulated male fertility in Arabidopsis

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Science China(Life Sciences) 2014年第1期57卷 145-154页

作者： HUANG Huang wang cuili TIAN HaiXia SUN Yu XIE DaoXin SONG SuSheng Tsinghua-Peking Center for Life Sciences MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University Beijing 100084 China

Jasmonate （JA） regulates various plant defense and developmental processes. The F-box protein CORONATINE INSENSITIVE 1 （COIl） perceives JA signals to mediate diverse plant responses including male fertility, root growth, antho- cyanin accumulation, and defense against abiotic and biotic stresses. In this study, we carried out genetic, physiological and biochemical analysis on a series of coil mutant alleles, and found that different amino acid mutations in COIl distinctively af- fect JA-regulated male fertility in Arabidopsis. All the JA responses are disrupted by the COIl mutations W467＂ in coil-l, Q343＂（coil-6）, G369E （coil-4）, G98D （coil-5）, G155E （coil-7）, D452A （coil-9） and L490A （coil-10）, though the coil-5 mutant （COIlG98D） contains adequate COIl protein （~60% of wild-type）. Interestingly, the low basal level of COIlE543K in the coil-8 mutant （-10% of wild-type COIl level） is sufficient for maintaining male fertility （-50% of wild-type fertility）; the coil-2 mutant with low level of COIlLz45F （-10% of wild-type） is male sterile under normal growth condition （22℃） but male fertile （~80% of wild-type fertility） at low temperature （16℃）; however, both coil-2 and coil-8 are defective in the other JA responses （root growth, anthocyanin accumulation, and plant response to the pathogen Pst DC3000 infection）.

关键词： COIl coil-2 coil-5 coil-8 jasmonate male fertility

Lipopolysaccharide-induced podocyte injury is regulated by calcineurin/NFAT and TLR4/MyD88/NF-κB signaling pathways through angiopoietin-like protein 4

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Genes & Diseases 2022年第6期9卷 443-455页

作者： Xiujin Shen Chunhua Weng Yucheng wang cuili wang Shi Feng Xiayu Li Heng Li Hong Jiang Haibing wang Jianghua Chen Kidney Disease Center the First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003PR China Key Laboratory of Kidney Disease Prevention and Control Technology Zhejiang ProvinceHangzhou 310063PR China National Key Clinical Department of Kidney Diseases Hangzhou 310005PR China Institute of Nephrology Zhejiang UniversityHangzhou 310020PR China The Third Grade Laboratory under the National State Administration of Traditional Chinese MedicineHangzhou 310005PR China Central Laboratory the First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006PR China

Podocyte injury is an important cause of ***-like protein 4(Angptl4)is a secreted glycoprotein and has a role in ***,the exact role of Angptl4 in podocyte injury and its upstream regulators has not been *** this study,we used lipopolysaccharide(LPS)-induced mice and cultured podocytes as podocyte injury *** results indicated that LPS increased the expression of podocyte Angptl4 in vivo and in ***,we showed that Angptl4 overexpression deteriorated LPS-induced podocyte injury by inducing podocyte cytoskeleton rearrangement,reducing the expression of synaptopodin while Angptl4 knockdown alleviated LPS-induced podocyte *** addition,we found that inhibitors and siRNA targeting TLR4/MyD88/NF-κB signaling inhibited the upregulation of Angptl4 in LPS-induced ***,inhibitors and siRNA targeting calcineurin/NFAT signaling also relieved LPS-induced Angptl4 expression and podocyte injury in vivo and in *** together,our study has elucidated that both of the TLR4/MyD88/NF-κB and calcineurin/NFAT signaling mediate the upregulation of Angptl4 in LPS-induced podocytes,which has important implications for further understanding the molecular mechanism of LPS-induced podocyte injury.

关键词： Angiopoietin-like protein 4 Calcineurin/NFAT LPS

The multifaceted role of placental growth factor in the pathogenesis and progression of bronchial asthma and pulmonary fibrosis:Therapeutic implications

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Genes & Diseases 2023年第4期10卷 1537-1551页

作者： Dan Huang Gege Liu Zhiyi Xu Shushu Chen cuili wang Dewei Liu Jiahao Cao Junfen Cheng Bin Wu Dong Wu Department of Respiratory and Critical Care Medicine Affiliated Hospital of Guangdong Medical UniversityZhanjiangGuangdong 524000China State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and TechnologyAvenida Wai LongTaipaMacao SAR 999078China Department of Respiratory and Critical Care Medicine The Second Affiliated Hospital of Guangdong Medical UniversityZhanjiangGuangdong 524000China

Placental growth factor(PlGF)is a glycosylated dimeric protein that is homologous to vascular endothelial growth factor(VEGF).PlGF expression is upregulated in patients with bronchial asthma,suggesting that it plays a role in the pathogenesis of *** asthma is characterized by chronic airway inflammation and airway hyperresponsiveness(AHR).After recurrent asthma attacks,pulmonary fibrosis develops and leads to airway remo-deling and a further decline in lung *** this review,we focused on the pivotal role of PlGF in chronic airway inflammation,AHR,and airway remodeling during bronchial ***,we summarized data showing that PlGF may be a potential therapeutic target in bronchial asthma.

关键词： AHR Airway remodeling Bronchial asthma Chronic airway inflammation PlGF

Telomere shortening in patients on long-term hemodialysis

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Chronic Diseases and Translational Medicine 2021年第4期7卷 266-275页

作者： Yucheng wang Siyu Chen Shi Feng cuili wang Hong Jiang Song Rong Haller Hermann Jianghua Chen Ping Zhang Kidney Disease Center The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang 310003China Key Laboratory of Nephropathy HangzhouZhejiang 310003China Kidney Disease Immunology Laboratory The Third-Grade LaboratoryState Administration of Traditional Chinese Medicine of ChinaHangzhouZhejiang 310003China Key Laboratory of Multiple Organ Transplantation Ministry of Health of ChinaHangzhouZhejiang 310003China Institute of Nephropathy Zhejiang UniversityHangzhouZhejiang 310003China Clinic for Kidney and Hypertension Diseases Hannover Medical SchoolLower Saxony30625Germany

Background:Leukocyte telomere length shortening is a characteristic of premature senescence,a process that can be accelerated by oxidative *** general,patients with end-stage renal disease undergoing regular hemodialysis(HD)are repeatedly exposed to oxidative *** undergoing HD tend to have cardiovascular diseases associated with oxidative stress and ***,we assumed that telomere length is associated with HD vintage and the degree of vascular ***:A total of 144 patients undergoing regular HD before kidney transplantation and 62 patients on hemodialysis,but not undergoing kidney transplantation,were *** measured common laboratory values,such as calcium,phosphate,and hemoglobin levels,and assessed the degree of vascular calcification in the *** leukocyte telomere length was measured using reverse transcription polymerase chain reaction,and Spearman correlation was used for correlation ***:The leukocyte telomere length was negatively associated with age(rho=-0.306,P<0.01);it was shorter in middle-aged patients than in young patients(13.48±4.80 vs.15.86±4.51,P<0.01).The telomere length was significantly different among patients aged 52-74 years in groups with different HD ***,the telomere length was positively associated with serum hemoglobin(Hb)levels in all patients(rho=0.290,P<0.01).There was a significant difference among patients divided into three groups according to the degree of anemia(17.09±5.64 vs.14.40±4.07 vs.13.99±3.95,P<0.01).Further,a significant difference was observed in the telomere length among patients with different degrees of vascular calcification(16.79±4.91 vs.13.61±2.82 vs.14.62±3.63 vs.10.71±3.74,P<0.01).The telomere length was shorter in the patients on hemodialysis who did not receive a kidney transplant than in the surgical patients(8.12±1.83 vs.14.33±4.63,P<0.01).Conclusion:This study demonstrated that the telomere length was significantly c

关键词： Telomere length Hemodialysis Chronic kidney disease Cardiovascular disease Hemoglobin

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西伯利亚红松球蚜药剂防治

东北林业大学学报 2018年第11期46卷 80-81,86页

作者：王翠丽内蒙古阿里河林业局呼伦贝尔165450

随着西伯利亚红松(Pinus sibirica)引种规模的扩大,过去危害红松(P. koraiensis)及偃松(P. pumila)的球蚜(Pineus cembrae pinikoreanus)也开始危害西伯利亚红松,已严重影响苗木的生长发育,确定经济有效的防治技术是生产急需解决的问题... 详细信息

随着西伯利亚红松(Pinus sibirica)引种规模的扩大,过去危害红松(P. koraiensis)及偃松(P. pumila)的球蚜(Pineus cembrae pinikoreanus)也开始危害西伯利亚红松,已严重影响苗木的生长发育,确定经济有效的防治技术是生产急需解决的问题。为此开展了吡虫啉、氧化乐果、敌敌畏、溴氰菊酯4种药剂3种稀释倍数的喷雾防治试验及成本分析。结果表明:采用8 000倍液的吡虫啉35.0%悬浮剂喷施幼苗及嫩稍防治效果最好,其杀虫率接近90%。此外,375倍液的溴氰菊酯(2.5%乳油)及氧化乐果(40.0%乳油)的杀虫率也较好,接近或超过85%,敌敌畏的防治效果较差,杀虫率55%～71%。4种杀虫剂中防治成本最高的是溴氰菊酯,达到2 070.0元·hm^(-2)·次^(-1),最低的是吡虫啉,仅为175.5元·hm^(-2)·次^(-1)。根据杀虫效果及防治成本,确认吡虫啉为最佳杀虫剂。

关键词：西伯利亚红松球蚜化学防治

河道堤岸防护施工技术在水利工程中的应用研究

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中国厨卫 2024年第5期23卷 173-175页

作者：王翠莉莘县水利局山东聊城252400

在水利工程中,河道堤岸防护施工是确保水资源管理、防洪和生态保护的关键。文章旨在通过研究水利工程中河道堤岸防护施工技术的应用,为相关工程的顺利实施提供参考。首先,分别从堤防土料填筑施工技术和河道护岸施工技术要点两方面探讨... 详细信息

在水利工程中,河道堤岸防护施工是确保水资源管理、防洪和生态保护的关键。文章旨在通过研究水利工程中河道堤岸防护施工技术的应用,为相关工程的顺利实施提供参考。首先,分别从堤防土料填筑施工技术和河道护岸施工技术要点两方面探讨了河道堤岸防护施工技术,其次,详细分析了河道堤岸防护工程施工存在的问题,特别是施工期间的生态保护、施工和质量控制以及管理手段和体制等,并提出了相应的对策,进而为河道堤岸防护施工的顺利进行提供参考。

关键词：水利工程河道堤岸防护

基于长标距FBG传感器的混凝土梁监测技术研究

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山西建筑 2018年第28期44卷 142-143页

作者：王淬砺福州市公路局长乐分局福建福州350200

为了在桥梁健康监测中准确获取结构变形,对基于长标距光纤光栅(FBG)传感器的变形监测技术进行了研究。以一简支混凝土梁为研究对象进行试验验证,结果表明长标距FBG传感技术能够准确获得结构变形。

关键词：桥梁结构光纤光栅长标距健康监测

高温胁迫对近江牡蛎Hsp90表达及CpG甲基化的影响

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广西科学 2019年第4期26卷 424-429页

作者：王翠丽南宁师范大学地理与海洋研究院南宁师范大学北部湾环境演变与资源利用教育部重点实验室

为了丰富广温性潮间带底栖生物的温度调控机制研究,本研究采用荧光定量PCR法、亚硫酸氢盐处理基因组DNA结合甲基化特异性PCR(Methylation specific PCR,MSP)法及染色质免疫共沉淀法,对40℃热胁迫24 h后,近江牡蛎(Crassostrea rivularis... 详细信息

为了丰富广温性潮间带底栖生物的温度调控机制研究,本研究采用荧光定量PCR法、亚硫酸氢盐处理基因组DNA结合甲基化特异性PCR(Methylation specific PCR,MSP)法及染色质免疫共沉淀法,对40℃热胁迫24 h后,近江牡蛎(Crassostrea rivularis)热休克蛋白90(Heat shock protein 90,Hsp90)的mRNA表达及其核心启动子甲基化的调控模式进行研究。结果显示:近江牡蛎成贝在40℃热胁迫24 h后,其消化腺、腮和心脏组织中Hsp90 mRNA表达显著升高(P0.05),灰度分析显示消化腺和心脏组织中的Hsp90核心启动子与RNA聚合酶Ⅱ(RNA polymeraseⅡ)结合增强,腮组织中的Hsp90核心启动子与RNA polymeraseⅡ出现弱结合。表明Hsp90是近江牡蛎抵御高温胁迫的主要表达基因。

关键词：高温胁迫近江牡蛎热休克蛋白90 核心启动子甲基化

菌胁迫对近江牡蛎子代母源C型凝集素mRNA表达及抗菌影响

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工业微生物 2023年第1期53卷 89-94页

作者：王翠丽南宁师范大学地理与海洋研究院广西南宁530000 南宁师范大学北部湾环境演变与资源利用教育部重点实验室广西南宁530000

为了揭示近江牡蛎(Crassostrea rivularis)C型凝集素(C-type lectin,CTL)母源免疫在子代胚胎发育早期过程中的免疫防御影响,文章运用荧光定量PCR、人工促排、免疫相关酶活测定等实验,开展了菌胁迫近江牡蛎子代母源CTL m RNA表达及抗菌... 详细信息

为了揭示近江牡蛎(Crassostrea rivularis)C型凝集素(C-type lectin,CTL)母源免疫在子代胚胎发育早期过程中的免疫防御影响,文章运用荧光定量PCR、人工促排、免疫相关酶活测定等实验,开展了菌胁迫近江牡蛎子代母源CTL m RNA表达及抗菌影响。结果表明:近江牡蛎子代胚胎发育早期可检测到母源CTL转录本,母贝分别在不同菌刺激后,其子代在受精卵、32细胞期、桑葚期、囊胚期CTL m RNA相对表达均出现不同程度的升高,与相应空白组比较差异显著(P<0.05);其子代在受精卵期、32细胞期的Cu/Zn-SOD活力和抑菌活性显著高于相应空白组(P<0.05);其子代在原肠胚期、担轮幼虫期累计死亡率均显著低于空白组(P<0.05)。结论:该近江牡蛎CTL具有母源免疫传代和子代免疫时效,当母源受到适当的免疫刺激可以增加其子代胚胎发育早期对相同病原感染的免疫防御能力。

关键词：菌胁迫近江牡蛎子代 C型凝集素抗菌活力