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10B-doped MCP detector developed for neutron resonance imaging at Back-n white neutron source

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Nuclear Science and Techniques 2024年第8期35卷 58-68页

作者： Qiang Li Li‑Jiao wang Jing‑Yu Tang Xiang‑Biao Qiu Zhen chen Mao‑Yuan zhao Chang‑Jun Ning Kai Pan wei Xu Tao Li Su‑Peng Lu Han Yi Rui‑Rui Fan Chang‑qing Feng Rong zhang xiao‑Yang Sun Qi An Hao‑Fan Bai Jiang‑Bo Bai Jie Bao ping Cao Qi‑ping chen Yong‑Hao chen Zeng‑Qi Cui An‑Chuan Fan Fan‑Zhen Feng Min‑Hao Gu Chang‑Cai Han Zi‑Jie Han Guo‑Zhu He Yong‑cheng He Yang Hong Yi‑wei Hu Han‑Xiong Huang wei Jiang Zhi‑Jie Jiang Zheng‑Yao Jin Ling Kang Bo Li Gong Li xiao Li Yang Li Jie Liu Rong Liu Shu‑Bin Liu Yi‑Na Liu Guang‑Yuan Luan Jie Ren Zhi‑Zhou Ren Xi‑Chao Ruan zhao‑Hui Song Kang Sun Zhi‑Xin Tan Sheng‑Da Tang Jin‑cheng wang Peng‑cheng wang zhao‑Hui wang Zhong‑wei Wen xiao‑Guang Wu Xuan Wu Cong Xia Yong‑Ji Yu Guo‑Hui zhang Hang‑Chang zhang Lin‑Hao zhang Qi‑wei zhang Xian‑Peng zhang Yu‑Liang zhang Yue zhang Zhi‑Yong zhang Zhi‑Hao Zhou Ke‑Jun Zhu Chong zou Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China State Key Laboratory of Particle Detection and Electronics Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Dongguan Songshan Lake Future School Dongguan 523808China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China North Night Vision Science and Technology(Nanjing)Research Institute Co.Ltd Nanjing 211106China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China Institute of Modern Physics Fudan UniversityShanghai 200433China Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China China Academy of Engineering Physics Institute of Nuclear Physics and ChemistryMianyang 621900China USTC archaeometry lab University of Science and Technology of ChinaHefei 230026China Northwest Institute of Nuclear Technology Xi’an 710024China University of Chinese Academy of Sciences Beijing 100049China China Shipbuilding Trading Co. LtdBeijing 100044China

Neutron resonance imaging(NRI)has recently emerged as an appealing technique for neutron *** complexity surpasses that of conventional transmission imaging,as it requires a high demand for both a neutron source and ***,the progression of NRI technology has been sluggish since its inception in the 1980s,particularly considering the limited studies analyzing the neutron energy range above *** white neutron source(Back-n)at the China Spallation Neutron Source(CSNS)provides favorable beam conditions for the development of the NRI technique over a wide neutron energy range from eV to ***-sensitive microchannel plates(MCP)have emerged as a cutting-edge tool in the field of neutron detection owing to their high temporal and spatial resolutions,high detection efficiency,and low *** this study,we report the development of a 10B-doped MCP detector,along with its associated electronics,data processing system,and NRI experiments at the *** heavy elements such as gold,silver,tungsten,and indium can be easily identified in the transmission images by their characteristic resonance peaks in the 1–100 eV energy range;the more difficult medium-weight elements such as iron,copper,and aluminum with resonance peaks in the 1–100 keV energy range can also be *** particular,results in the neutron energy range of dozens of keV(Aluminum)are reported here for the first time.

关键词： Neutron resonance imaging 10B doped MCP detector White neutron source Sample nuclide identification

咳喘停袋泡颗粒抑制IL-33/ILC2s和致病性T细胞干预过敏性气道炎症的作用机制

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中国药理学通报 2024年第7期40卷 1350-1357页

作者：邹楠婷吴招闫晓东张春菲张浩洪莫庆艳巨鸣谦徐金柱万春平第一临床医学院和中药学院云南中医药大学云南昆明650500 玉溪市中医医院呼吸内科云南玉溪653100 镇雄县中医医院药剂科云南昭通657200

目的研究咳喘停袋泡颗粒(Kechuanting,KCT)抑制IL-33/ILC2s和致病性T细胞干预过敏性气道炎症的作用机制。方法网络药理学分析KCT干预哮喘的潜在靶点和机制。以OVA诱导过敏性哮喘模型小鼠。组织病理染色观察肺损伤变化;ELISA和荧光定量PC... 详细信息

目的研究咳喘停袋泡颗粒(Kechuanting,KCT)抑制IL-33/ILC2s和致病性T细胞干预过敏性气道炎症的作用机制。方法网络药理学分析KCT干预哮喘的潜在靶点和机制。以OVA诱导过敏性哮喘模型小鼠。组织病理染色观察肺损伤变化;ELISA和荧光定量PCR分别检测Th2型哮喘中关键炎症因子及其mRNA表达水平;Western blot检测MAPK通路中相关蛋白磷酸化水平;流式细胞术检测ILC2s、Th1、Th17、Th2和Treg细胞的比例。结果网络药理学鉴定出KCT治疗哮喘的227个主要活性成分和143个共同靶点,主要富集到MAPK和IL-17等信号通路。进一步验证实验显示,KCT干预明显减轻了哮喘小鼠肺部炎症损伤程度,减少B细胞数量及IL-4、TNF-α、TGF-β的产生,下调肺组织中JNK磷酸化水平以及Il-33、Bcl11b、Rorα、Tcf-7、Jun、MAPK3、MAPK14 mRNA表达。KCT干预减少了哮喘小鼠肺和脾中ILC2s和Th17数量,抑制肺中Th2细胞浸润。结论KCT对过敏性哮喘气道炎症有较好的治疗作用,其机制与抑制IL-33/ILC2s通路、致病性T细胞亚群和JNK-MAPK信号通路相关。

关键词：咳喘停袋泡颗粒过敏性哮喘气道炎症 ILC2s Th2细胞 CD4^(+)T细胞

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Monitoring the little fire ant,Wasmannia auropunctata(Roger 1863),in the early stage of its invasion in China:Predicting its geographical distribution pattern under climate change

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农业科学学报:英文版 2023年第9期22卷 2783-2795页

作者： zhao Hao-xiang XIAN xiao-qing GUO Jian-yang YANG Nian-wan zhang Yan-ping chen Bao-xiong HUANG Hong-kun LIU wan-xue State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural ScienceBeijing 100193P.R.China Western Agricultural Research Center Chinese Academy of Agricultural SciencesChangji 831100P.R.China Rural Energy and Environment Agency Ministry of Agriculture and Rural AffairsBeijing 100193P.R.China

Invasive alien ants(IAAs)are among the most aggressive,competitive,and widespread invasive alien species(IAS)*** auropunctata,the greatest IAAs threat in the Pacific region and listed in“100 of the world’s worst IAS”,has established itself in many countries and on islands *** populations of *** were recently reported in southeastern China,representing a tremendous potential threat to China’s agricultural,economic,environmental,public health,and social *** the potential geographical distribution(PGD)of *** in China can illustrate areas that may potentially face invasion ***,based on the global distribution records of *** and bioclimatic variables,we predicted the geographical distribution pattern of *** in China under the effects of climate change using an ensemble model(EM).Our findings showed that artificial neural network(ANN),flexible discriminant analysis(FDA),gradient boosting model(GBM),Random Forest(RF)were more accurate than categorical regression tree analysis(CTA),generalized linear model(GLM),maximum entropy model(MaxEnt)and surface distance envelope(SRE).The mean TSS values of ANN,FDA,GBM,and RF were 0.820,0.810,0.843,and 0.857,respectively,and the mean AUC values were 0.946,0.954,0.968,and 0.979,*** mean TSS and AUC values of EM were 0.882 and 0.972,respectively,indicating that the prediction results with EM were more reliable than those with the single *** PGD of *** in China is mainly located in southern China under current and future climate *** climate change,the PGD of *** in China will expand to higher-latitude *** annual temperature range(bio7)and mean temperature of the warmest quarter(bio10)were the most significant variables affecting the PGD of *** in *** PGD of *** in China was mainly attributed to temperature variables,such as the annual temperature range(bio7

关键词： invasive alien ants potential geographical distribution Wasmannia auropunctata climate change Ensemble model

CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin chen Danping chen Fuqing chen Fusan chen Gang chen Guoming chen Hua-Xing chen Huirun chen Jinhui chen Ji-Yuan chen Kai chen Mali chen Mingjun chen Mingshui chen Ning chen Shanhong chen Shanzhen chen Shao-Long chen Shaomin chen Shiqiang chen Tianlu chen wei chen Xiang chen xiaoyu chen Xin chen Xun chen Xurong chen Ye chen Ying chen Yukai chen Zelin chen Zilin chen Gang chen Boping chen Chunhui chen Hok Chuen cheng Huajie cheng Shan cheng Tongguang cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui xiaohao Cui zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De sio Elisa Del Core Shuangxue Deng wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding siqin Dingl Yadong Ding Haiyi Dong Jianin AGH University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa chengdu Kaitengsifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China University of Geosciences Beijing China University of Geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China University Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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实施理论——常态化过程理论的解读

护理学报 2024年第5期31卷 39-43页

作者：李春萍张兰萍王晓辉钟乐欣杨薇赵青吕森森李嘉琪谢昀筠黄婉晴陈晓珊徐东南方医科大学公共卫生学院广东广州510515 南方医科大学卫生管理学院卫生管理系Acacia实施科学实验室广东广州510515 南方医科大学世界卫生组织研究中心广东广州510515 兰州大学公共卫生学院甘肃兰州730000 贵州医科大学公共卫生与健康学院环境污染与疾病监控教育部重点实验室贵州贵阳561113 南方医科大学皮肤病医院全球健康研究中心广东广州510515

目的常态化过程理论(Normalization Process Theory,NPT)是实施研究中常用的理论之一,但较难理解。本文将详细介绍NPT,为研究者理解NPT提供指引。方法通过检索与NPT理论相关的文献并结合团队实际使用NPT的体验,描述NPT的起源、开发与... 详细信息

目的常态化过程理论(Normalization Process Theory,NPT)是实施研究中常用的理论之一,但较难理解。本文将详细介绍NPT,为研究者理解NPT提供指引。方法通过检索与NPT理论相关的文献并结合团队实际使用NPT的体验,描述NPT的起源、开发与演变、核心要素解读、运用并讨论其特点。结果 NPT以新技术在日常工作中的嵌入(即常态化)为实施结局,指出这个结局是实施者、实施对象和实施环境相互作用的过程。经过20余年的演变,NPT最终包含4个维度和16个构念。结论 NPT逐步完善,为实施科学提供了重要的理论基础;但NPT比较抽象,且存在逻辑和概念的不足,研究者需要在透彻了解其含义的基础上灵活使用。

关键词：常态化过程理论(NPT) 实施科学实施研究循证实践

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2004-2005年中国肺癌死亡情况分析

中华预防医学杂志 2010年第5期44卷 378-382页

作者：陈万青张思维邹小农赵平中国医学科学院肿瘤医院肿瘤研究所全国肿瘤防治研究办公室北京100021

目的分析我国2004--2005年肺癌死亡的分布特点及流行趋势。方法2004--2005年全国第3次死因回顾抽样调查共调查142660482人年，其中男性72970241人年，女性69690241人年。从中抽取气管、支气管和肺恶性肿瘤死亡记录。根据不同性别，城乡... 详细信息

目的分析我国2004--2005年肺癌死亡的分布特点及流行趋势。方法2004--2005年全国第3次死因回顾抽样调查共调查142660482人年，其中男性72970241人年，女性69690241人年。从中抽取气管、支气管和肺恶性肿瘤死亡记录。根据不同性别，城乡和东、中、西部地区的划分，分别计算粗死亡率、年龄别死亡率、地区别死亡率、肿瘤死因构成比、中国人口标化死亡率（简称中标率）、世界人口标化死亡率（简称世标率）及死因顺位，并与中国前2次死因回顾调查（1973—1975年和1990--1992年）的肺癌死亡统计指标进行比较。结果2004--2005年全国死因抽样调查肺癌共死亡43993例，粗死亡率为30．84／10万，中标率为20．24／10万，世标率为27．62／10万，占被调查全部恶性肿瘤死亡（193841例）的22．70％；男性死亡30167例，粗死亡率为41．34／10万；女性死亡13826例，粗死亡率为19．84／10万。肺癌年龄别死亡率随年龄的增加而增加。城市中肺癌粗死亡率为40．98／10万（19628／47899806），在城市肿瘤死因顺位中排第1位，占城市全部恶性肿瘤死亡（71936例）的27．29％；农村粗死亡率为25．71／10万（24365／94760676），仅次于肝癌排第2位，占农村全部恶性肿瘤死亡（121905例）的19．99％。全国东、中、西部不同地区肺癌的死亡率也明显不同，粗死亡率最高的是东部地区，为37．85／10万（19893／52556694）；西部地区最低为21．76／10万（8774／40322563）。肺癌粗死亡率与1990--1992年第2次死因调查（粗死亡率为17．54／10万）相比上升了75．83％，与1973--1975年第1次调查（粗死亡率为5．46／10万）比较，上升了464．84％，呈明显上升趋势。结论肺癌是影响我国城市居民健康的主要恶性肿瘤，其死亡水平呈上升趋势。

关键词：肺肿瘤死亡率人口统计学

基于白光中子源的^(197)Au中子辐射俘获截面测量及共振参数分析

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物理学报 2024年第7期73卷 104-113页

作者：罗淏天张奇玮栾广源王晓宇邹翀任杰阮锡超贺国珠鲍杰孙琪黄翰雄王朝辉吴鸿毅顾旻皓余滔解立坤陈永浩安琪白怀勇鲍煜曹平陈昊磊陈琪萍陈裕凯陈朕崔增琪樊瑞睿封常青高可庆韩长材韩子杰何泳成洪杨黄蔚玲黄锡汝季筱璐吉旭阳蒋伟江浩雨姜智杰敬罕涛康玲康明涛李波李超李嘉雯李论李强李晓李样刘荣刘树彬刘星言穆奇丽宁常军齐斌斌任智洲宋英鹏宋朝晖孙虹孙康孙晓阳孙志嘉谭志新唐洪庆唐靖宇唐新懿田斌斌王丽娇王鹏程王琦王涛峰文杰温中伟吴青彪吴晓光吴煊羊奕伟易晗于莉于永积张国辉张林浩张显鹏张玉亮张志永赵豫斌周路平周祖英朱丹阳朱科军朱鹏朱兴华中国原子能科学研究院核数据重点实验室北京102413 中国科学院高能物理研究所北京100049 散裂中子源科学中心东莞523803 核探测与核电子学国家重点实验室北京100049 中国科学技术大学近代物理系核探测与核电子学国家重点实验室合肥230026 北京大学物理学院核物理与核技术国家重点实验室北京100871 中国工程物理研究院核物理与化学研究所绵阳621900 西北核技术研究院西安710024 中国科学院大学北京100049 中国科学技术大学工程与应用物理系合肥230026 北京航空航天大学物理学院北京100083 华能山东石岛湾核电有限公司荣成264312

中子辐射俘获反应在反应堆运行、核装置设计及核天体物理研究中起重要的作用.4πBaF_(2)探测装置有着高时间分辨能力、低中子灵敏度、高探测效率等优点,适合开展中子辐射俘获反应截面数据的测量.中国原子能科学研究院核数据重点实验室... 详细信息

中子辐射俘获反应在反应堆运行、核装置设计及核天体物理研究中起重要的作用.4πBaF_(2)探测装置有着高时间分辨能力、低中子灵敏度、高探测效率等优点,适合开展中子辐射俘获反应截面数据的测量.中国原子能科学研究院核数据重点实验室建立了伽马全吸收装置(Gamma total absorption facility,GTAF),该装置用28块六棱BaF_(2)晶体和12块五棱BaF_(2)晶体构成了外径25 cm,内径10 cm的球壳,覆盖了95.2%的立体角.利用GTAF在中国散裂中子源Back-n束线上,测量了197Au(n,γ)的反应截面数据.测量数据通过能量筛选、PSD方法、晶体多重性筛选进行了初步本底扣除,随后结合对^(nat)C及空样品的测量数据对本底进行了分析及扣除,获得了197Au俘获反应的产额,利用SAMMY程序拟合得到了^(197)Au在1—100 e V的共振能量、中子共振宽度和伽马共振宽度参数.实验测量结果与ENDF/B-VIII.0数据库符合良好,其共振参数存在一定差异,分析原因可能与GTAF能量分辨率、Back-n的中子能谱测量精度、以及实验本底扣除方法相关,这也是下一步工作的重点.

关键词：中子辐射俘获截面共振参数白光中子源伽马全吸收装置

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基于TMT蛋白质组学研究黄芪甲苷干预脓毒症肺损伤作用靶点的研究

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云南中医中药杂志 2024年第2期45卷 83-89页

作者：张春菲张浩洪李晓红吴招邹楠婷杨海浩巨鸣谦莫庆艳万春平云南中医药大学云南昆明650500 楚雄州中医医院云南楚雄675000

目的采用TMT蛋白质组学研究黄芪甲苷对脓毒症小鼠肺组织蛋白组学的影响,为其临床应用治疗脓毒症提供科学依据。方法采用盲肠结扎穿刺术(Cecal Ligation-Peferation,CLP)复制脓毒症小鼠模型,随机分为假手术组、模型组、黄芪甲苷组。H&E... 详细信息

目的采用TMT蛋白质组学研究黄芪甲苷对脓毒症小鼠肺组织蛋白组学的影响,为其临床应用治疗脓毒症提供科学依据。方法采用盲肠结扎穿刺术(Cecal Ligation-Peferation,CLP)复制脓毒症小鼠模型,随机分为假手术组、模型组、黄芪甲苷组。H&E染色检测肺组织病理损伤;流式细胞术检测B细胞表达;提取肺组织蛋白,运用TMT蛋白质组学技术比较组间差异蛋白的表达,并进行生物信息学分析。结果与假手术组比较,模型组肺组织炎症细胞浸润严重,结构塌陷;黄芪甲苷干预显著减轻脓毒症模型小鼠肺组织病理损伤。与模型组比较,黄芪甲苷药物干预组小鼠脾淋巴细胞B细胞(B220+)表达无明显影响(P>0.05)。TMT蛋白质组学结果分析肺组织蛋白差异成分,结果共鉴定出33个显著性差异蛋白,黄芪甲苷干预上调17个蛋白,下调16个蛋白。GO富集分析显示,黄芪甲苷主要干预生物功能过程,其中与脓毒症肺损伤密切相关差异主要蛋白是介导黏膜免疫的IgA产生通路。结论黄芪甲苷对脓毒症诱导的肺损伤具有显著作用,其效应机制主要与干预B细胞分泌的IgA介导的黏膜免疫有关,该研究为黄芪临床治疗脓毒症奠定了基础。

关键词：蛋白质组学脓毒症黄芪甲苷免疫球蛋白A

新型查尔酮衍生物MW-9抑制MAPK信号通路治疗小鼠溃疡性结肠炎

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中国药理学通报 2024年第3期40卷 514-520页

作者：吴招邹楠婷张春菲张浩洪莫庆艳巨鸣谦胥兴彩毛泽伟万春平云南中医药大学中药学院云南昆明650500 云南中医药大学基础医学院云南昆明650500 云南中医药大学第三附属医院脾胃肝病科云南昆明650500

目的揭示新型查耳酮衍生物MW-9抑制MAPK通路治疗溃疡性结肠炎的效应机制,为查尔酮衍生物MW-9治疗溃疡性结肠炎提供科学依据。方法建立细菌脂多糖诱导的小鼠巨噬细胞RAW264.7炎症模型,MTT法检测MW-9对细胞活度的影响;Griess法检测细胞NO... 详细信息

目的揭示新型查耳酮衍生物MW-9抑制MAPK通路治疗溃疡性结肠炎的效应机制,为查尔酮衍生物MW-9治疗溃疡性结肠炎提供科学依据。方法建立细菌脂多糖诱导的小鼠巨噬细胞RAW264.7炎症模型,MTT法检测MW-9对细胞活度的影响;Griess法检测细胞NO含量;ELISA法检测细胞培养上清及血清中炎症因子IL-6、IL-1β和TNF-α的水平。构建葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠模型,记录体质量;HE染色检测结肠病理损伤程度;Western blot测定结肠组织MAPK通路关键蛋白p38、ERK1/2、JNK的磷酸化水平。结果MW-9明显抑制RAW264.7细胞NO的产生,其IC50为20.47 mg·L^(-1),抑制炎症因子IL-6、IL-1β和TNF-α的高表达(P<0.05)。MW9在低于6 mg·L^(-1)的浓度下无明显细胞毒性。MW-9治疗后,小鼠体质量下降减缓,可降低血清中促炎因子IL-6、IL-1β和TNF-α水平;与模型组比较,MW-9明显降低MAPK通路中磷酸化p38和ERK1/2蛋白的表达水平。结论新型查耳酮衍生物MW-9在体内外具有明显的抗炎活性,其治疗溃疡性结肠炎作用机制可能与抑制MAPK信号通路有关。

关键词：查尔酮 MW-9 炎症溃疡性结肠炎 RAW264.7细胞 MAPK信号通路