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Agricultural Sciences in China 2011年第4期10卷 619-630页

作者： wan zhong-ying tong hui-li li qing-zhang gao xue-jun Key Laboratory of Dairy Science Ministry of Education~Northeast Agricultural University Harbin 150030 P.R.China

The galactopoietic mechanism of Vaccaria segetalis is still unknown. Understanding dibutyl phthalate （DBP） separated from Vaccaria segetalis on the expression of lactation signal transduction genes of mammary gland epithelial cells, including prlr, erα, akt1, socs2, pparγ and elf5, will be helpful to reveal the molecular mechanism. Western blot and qRT- PCR were used to study the change of prlr, erα, akt, socs2, pparγ, and elf5 expression at mRNA and protein level. Co- localization expression of prolactin receptor （PRLR） and estrogen receptor α （ERα） was observed by immunofluorescence; the expression changes of miRNAs （21, 125b, 143, and 195） and the secretion of β-casein and lactose were detected by qRT-PCR and RP-HPLC. The results showed that Vaccaria segetalis active compound had similar fuctions as estrogen and/or prolactin （PRL） in dairy cow mammary gland epithelial cells （DCMECs）, increased the expressions of prlr, erα, akt1, and elf5 genes, while repressed pparγ expressions. DBP promoted socs2 mRNA expression, but its protein expressions were repressed. Furthermore, both DBP and PRL could repress the expressions of miRNA-125b, miRNA-143 and miRNA- 195 in DCMECs. DBP could repress the expression of miRNA-21, while the influence of PRL on miRNA-21 was not certain. DBP could promote the lactation ability of DCMECs by regulating the ER and PRLR cellular signal transduction pathway.

关键词： Vaccaria segetalis DBP dairy cow mammary gland epithelial cells signal transduction miRNAs

Metabolic Regulation of Mammary Gland Epithelial Cells of Dairy Cow by Galactopoietic Compound Isolated from Vaccariae segetalis

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Agricultural Sciences in China 2011年第7期10卷 1106-1116页

作者： tong hui-li gao xue-jun li qing-zhang liU Jie li Nan wan zhong-ying Key Laboratory of Dairy Science Ministry of Education/Life College Northeast Agricultural University Harbin 150030 P.R.China

In previous experiment, we isolated a compound dibutyl phthalate （DBP） from Vaccaria segetalis which had galactopoietic function on mammary gland epithelial cells of dairy cow （DCMECs）. In this experiment, we ascertained the metabolic regulation function of DBP on DCMECs. Many genes related to lactation including Stat5, AMPK, b-casein, Glut1, SREBP-1, PEPCK, and ACC were detected by real-time PCR. Furthermore, Stat5 and AMPK were detected by Western blot and immunofluorescence co-localization, respectively. The results showed that DBP stimulates the expression of Stat5 and p-Stat5, thus activates Stat5 cell signal transduction pathway and stimulates b-casein synthesis. DBP also raises the activities of Glut1 and AMPK to stimulate glucose uptake and glycometabolism and activates the expression of AMPK downstream target genes PEPCK and ACC and expression of SREBP-1 to stimulate milk fat synthesis. In addition, the activities of HK, G-6-PDH, ICDH, ATPase, and energy charges were stimulated by DBP to increase the energy metabolism level of DCMECs. The results showed DBP stimulates energy metabolism related to galactopoietic function in DCMECs.

关键词： Vaccaria segetalis DBP dairy cow mammary gland epithelial cell metabolic regulation

Effect of Semen vaccariae and Taraxacu mogono on Cell Adhesion of Bovine Mammary Epithelial Cells

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Journal of Integrative Agriculture 2012年第12期11卷 2043-2050页

作者： tong Jin-jin li Ye liU Rong gao xue-jun li qing-zhang Key Laboratory of Dairy Science Ministry of Education/College of Life Science Northeast Agricultural University

The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and β-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, β-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferfated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher （P〈0.05） in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly （P〈0.05）. In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, β-catenin and CycinD1 in the Wnt signaling pathway.

关键词： E-cadherin β-catenin Semen vaccariae Taraxacu mogono bovine mammary epithelial cells

The Effects of Insulin and Prolactin on an Epithelial Cell line from Mammary Gland of Dairy Goat

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畜牧兽医学报 2009年第S1期40卷 47-50页

作者： tong hui-li gao xue-jun li qing-zhang YAN Yun-qin Northeast Agricultural University

Mammary epithelial cells with lactational function can be a valuable cellular model for research of the development and regulation of the mammary *** paper describes some aspects of function of an epithelial cell line from the mammary gland of the dairy ***-PAGE,triglyceride and lactose content of cultured cells were used to assess synthetic function of cells and the effects of exposure to insulin and *** show that goat mammary epithelial cells can synthesize fat,proteins and lactose when they were cultured in DMEM-F12 medium with added EGF,IGF-1,ITS and *** were no obvious changes after 48h treatment with additional *** added to the basal medium significantly increased synthesis of proteins and lactose.A mammary gland epithelial cell line from goats which has lactational function has been *** outcome provides a valuable and convenient model system.

关键词： insulin prolactin dairy goat mammary gland epithelial cell lactation function

Population genomics reveals that natural variation in PRDM16 contributes to cold tolerance in domestic cattle

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Zoological Research 2022年第2期43卷 275-284页

作者： Chun-Long Yan jun lin Yuan-Yuan Huang qing-Shan gao Zheng-Yu Piao Shou-li Yuan li Chen xue Ren Rong-Cai Ye Meng Dong Han-lin zhang hui-Qiao Zhou Xiao-Xiao Jiang wan-Zhu Jin Xu-Ming Zhou Chang-Guo Yan College of Agriculture Yanbian UniversityYanjiJilin 133000China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China North-East Cold Region Beef Cattle Science&Technology Innovation Ministry of Education Engineering Research Center Yanbian UniversityYanjiJilin 133000China Annoroad Gene Technology Co.Ltd Beijing 100176China

Environmental temperature serves as a major driver of adaptive changes in wild *** discover the mechanisms underpinning cold tolerance in domestic animals,we sequenced the genomes of 28 cattle from warm and cold areas across *** characterizing the population structure and demographic history,we identified two genetic clusters,i.e.,northern and southern groups,as well as a common historic population peak at 30 kilo years *** scan of cold-tolerant breeds determined potential candidate genes in the thermogenesis-related pathways that were under ***,functional analysis identified a substitution of PRDM16(p.P779 L)in northern cattle,which maintains brown adipocyte formation by boosting thermogenesis-related gene expression,indicating a vital role of this gene in cold *** findings provide a basis for genetic variation in domestic cattle shaped by environmental temperature and highlight the role of reverse mutation in livestock species.

关键词： Population genomics Cattle Cold tolerance PRDM16 Brown adipose tissue

TLR8 in the Trigeminal Ganglion Contributes to the Maintenance of Trigeminal Neuropathic Pain in Mice

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Neuroscience Bulletin 2021年第4期37卷 550-562页

作者： lin-Xia Zhao Ming Jiang xue-Qiang Bai De-li Cao Xiao-Bo Wu Jing zhang Jian-Shuang Guo tong-tong Chen Juan wang Hao Wu Yong-Jing gao Zhi-jun zhang Department of Human Anatomy School of MedicineNantong UniversityNantong 226001China Institute of Pain Medicine Institute of Nautical MedicineNantong UniversityNantong 226019China Department of Otolaryngology Head Neck Surgery The Affiliated Hospital of Nantong UniversityNantong 226001China Co-innovation Center of Neuroregeneration Nantong UniversityNantong 226001China

Trigeminal neuropathic pain(TNP)is a significant health problem but the involved mechanism has not been completely ***-like receptors(TLRs)have recently been demonstrated to be expressed in the dorsal root ganglion and involved in chronic ***,we show that TLR8 was persistently increased in the trigeminal ganglion(TG)neurons in model of TNP induced by partial infraorbital nerve ligation(pIONL).In addition,deletion or knockdown of Tlr8 in the TG attenuated pIONL-induced mechanical allodynia,reduced the activation of ERK and p38-MAPK,and decreased the expression of pro-inflammatory cytokines in the ***,intra-TG injection of the TLR8 agonist VTX-2337 induced pain ***-2337 also increased the intracellular Ca^(2+)concentration,induced the activation of ERK and p38,and increased the expression of pro-inflammatory cytokines in the *** data indicate that TLR8 contributes to the maintenance of TNP through increasing MAPK-mediated *** TLR8 signaling may be effective for the treatment of TNP.

关键词： TLR8 ERK p38 Pro-inflammatory cytokine Trigeminal ganglion Trigeminal neuropathic pain Mouse

Analysis of electromagnetic pulses generated from ultrashort laser irradiation of solid targets at CLAPA

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Chinese Physics B 2022年第2期31卷 489-495页

作者： Yi-lin Xu Dong-Yu li Ya-Dong Xia Si-Yuan zhang Min-Jian Wu tong Yang jun-gao Zhu Hao Cheng Chuan-Ke wang Chen lin Ting-Shuai li xue-qing Yan School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China State Key Laboratory of Nuclear Physics and Technology and Key Laboratory of HEDP of the Ministry of EducationCAPTPeking UniversityBeijing 100871China Research Center of Laser Fusion China Academy of Engineering Physics(CAEP)Mianyang 621900China

Electromagnetic pulses(EMPs)produced by the interaction of a TW femtosecond laser with solid targets at the Compact Laser Plasma Accelerator(CLAPA)are measured and *** statistical results confirm that the intensities of the EMPs are closely related to both target material and *** signal of the titanium target is more abundant than that of the copper target with the same thickness,and the intensity of EMP is positively correlated with the target thickness for aluminium *** the boosted EMP radiations,the energy of accelerated protons is also simultaneously *** addition,EMPs emitted from the front of the target exceed those from the rear,which are also pertinent to the specific target *** resonant waveforms in the target chamber are analyzed using the fast Fourier transform,and the local resonance and the attenuation lead to changes of the frequency spectra of EMPs with variation of detecting positions,which is well supported by the modeling *** findings are beneficial to gaining insight into the mechanism of EMP propagation in a typical target chamber and providing more information for EMP shielding design.

关键词： laser driven proton accleration electromagnetic pulses laser target

Galactopoietic Activity of Dibutyl Phthalate Isolated from Vaccaria segetalis

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Journal of Northeast Agricultural University(English Edition) 2013年第4期20卷 28-33页

作者： tong hui-li gao xue-jun Sheng Zun-lai li qing-zhang li Shu-feng li Nan liu Jie Yan Yun-qin College of life Sciences Northeast Agricultural University Key Laboratory of Dairy Science of Education Ministry Northeast Agricultural University College of Veterinary Medicine Northeast Agricultural University

A galactopoietic compound, identified as dibutyl phthalate（DBP）, was isolated from Vaccaria segetalis. The activity of DBP on lactation ability of dairy cow mammary gland epithelial cells（DCMECs） cultured in vitro and dairy cow was evaluated. Results showed that DBP could promote cell viability, proliferation ability, lactose and β-casein secretion of DCMECs, which could also raise the milk yields of dairy cows significantly.

关键词： Vaccaria segetalis DBP galactopoietic activity dairy cow mammary gland epithelial cell

Porous organic polymers: a progress report in China

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Science China Chemistry 2023年第3期66卷 620-682页

作者： qing Hao You Tao xuesong Ding Yajie Yang Jie Feng Rui-Lei wang xue-Ming Chen Guan-Le Chen Xiaomeng li Huang OuYang Xunliang Hu Jia Tian Bao-Hang Han Guangshan Zhu Wei wang Fan zhang Bien Tan Zhan-Ting li Dong wang li-jun wan Key Laboratory of Molecular Nanostructure and Nanotechnology Beijing National Laboratory for Molecular SciencesCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education and Faculty of Chemistry Northeast Normal UniversityChangchun 130024China State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou Magnetic Resonance CenterLanzhou UniversityLanzhou 730000China School of Chemistry and Chemical Engineering State Key Laboratory of Metal Matrix CompositesShanghai Electrochemical Energy Devices Research CenterShanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Material Chemistry for Energy Conversion and Storage Ministry of EducationSchool of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhan 430074China Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China Department of Chemistry Fudan UniversityShanghai 200438China University of Chinese Academy of Sciences Beijing 100049China

Porous organic polymers(POPs) are porous materials composed of light elements such as C, H, N, and O. The benign characters,including large surface area, good physical and chemical stability, well-defined chemical composition, wide ranges of monomer selection, and strong designability, have made POPs one of the frontiers in materials research. In this review, we discussed the design and synthesis of various POP materials that mainly led by Chinese scientists, including conjugated microporous polymers(CMPs), porous aromatic frameworks(PAFs), and hypercrosslinked porous polymers(HCPs), as well as crystalline POPs comprised of covalent organic frameworks(COFs) and a special class of COFs with triazine rings, covalent triazine frameworks(CTFs), and supramolecular organic frameworks(SOFs), and sorted out their main applications in adsorption, separation,catalysis, and electrochemistry fields.

关键词： Porous porous frontier

A herbal pair of Scutellaria barbata D. Don and Scleromitrion diffusum(Willd.) R.J. wang induced ferroptosis in ovarian cancer A2780 cells via inducing heme catabolism and ferritinophagy

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Journal of Integrative Medicine 2024年第6期22卷 665-682页

作者： Zhen wang Min liu Guang-xing li liu zhang Kai-yue Ding Si-qi li Bing-qing gao Peng Chen Hyok-Chol Choe Lun-yue Xia Yu-tong Yang Yi liu xue Sui jun-nan Ma lin zhang Institute (College) of Integrative Medicine Dalian Medical University Department of General Surgery the First Affiliated Hospital of Dalian Medical University Advanced Institute of Medical Sciences Dalian Medical University Department of Clinical Medicine Sinuiju Medical University

Objective: Despite the combination of Scutellaria barbata D. Don and Scleromitrion diffusum(Willd.) ***(SB-SD) being a recognized Chinese medicinal herbal pair that is commonly used in the treatment of ovarian cancer, there is a poor understanding of their pharmacological mechanisms. This study examines the antitumor properties and potential mechanisms of SB-SD on human ovarian cancer A2780 cells through a multi-omics approach, establishing a pharmacological basis for clinical ***: A range of mass ratios and reagents were used in the hot reflux extraction of SB-SD. The inhibitory effect of the SB-SD extracts on A2780 cell proliferation was assessed using the cell-counting kit 8assay. A zebrafish tumor implantation model was used to evaluate the effects of SB-SD extracts on tumor growth and metastasis in vivo. Transcriptomics and proteomics were used to investigate alterations in biological pathways in A2780 cells after treatment with different concentrations of SB-SD extract. Cell cycle,cell apoptosis, intracellular free iron concentration, intracellular reactive oxygen species(ROS) concentration, malondialdehyde(MDA), and mitochondrial membrane potential were measured. Real-time quantitative reverse transcription polymerase chain reaction and Western blotting were utilized to investigate the effects of heme catabolism and ferritinophagy on ferroptosis induced by SB-SD extract in A2780 ***: The 70% ethanol extract of SB-SD(a mass ratio of 4:1) inhibited A2780 cell proliferation significantly with a half maximal inhibitory concentration of 660 μg/m L in a concentration-and timedependent manner. Moreover, it effectively suppressed tumor growth and metastasis in a zebrafish tumor implantation model. SB-SD extract induced the accumulation of free iron, ROS, MDA, and mitochondrial damage in A2780 cells. The mechanisms might involve the upregulated expression of ferritinophagyrelated genes microtubule-associated protein 1 light chain 3, auto

关键词： Ferritinophagy Ferroptosis Heme catabolism Ovarian cancer Proteomic Scleromitrion diffusum (Willd.) R.J. wang Scutellaria barbata D. Don Transcriptomic